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![Western Blot: NCOA4 Antibody - BSA Free [NBP3-18136] - Western Blot Validation in Rat Tissues. Loading: 15 ug of lysates per lane. Antibodies: NCOA4 NBP3-18136, 1 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.](http://images.novusbio.com/fullsize/NCOA4-Antibody---BSA-Free-Western-Blot-NBP3-18136-img0003.jpg "Western Blot: NCOA4 Antibody - BSA Free [NBP3-18136] - Western Blot Validation in Rat Tissues. Loading: 15 ug of lysates per lane. Antibodies: NCOA4 NBP3-18136, 1 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.")
![Immunocytochemistry/Immunofluorescence: NCOA4 Antibody - BSA Free [NBP3-18136] - Immunofluorescent analysis of 4% paraformaldehyde-fixed Daudi cells labeling NCOA4 with NBP3-18136 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/NCOA4-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NBP3-18136-img0004.jpg)
![Immunohistochemistry-Paraffin: NCOA4 Antibody - BSA Free [NBP3-18136] - Immunohistochemical analysis of paraffin-embedded mouse tissue using anti-NCOA4 antibody (NBP3-18136) at 1 ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/NCOA4-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NBP3-18136-img0006.jpg)
![Western Blot: NCOA4 Antibody - BSA Free [NBP3-18136] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: NCOA4 NBP3-18136, 0.5 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.](http://images.novusbio.com/fullsize/NCOA4-Antibody---BSA-Free-Western-Blot-NBP3-18136-img0001.jpg)
![Western Blot: NCOA4 Antibody - BSA Free [NBP3-18136] - Western Blot Validation in Mouse Tissues. Loading: 15 ug of lysates per lane. Antibodies: NCOA4 NBP3-18136, 1 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.](http://images.novusbio.com/fullsize/NCOA4-Antibody---BSA-Free-Western-Blot-NBP3-18136-img0002.jpg)
![Immunohistochemistry-Paraffin: NCOA4 Antibody - BSA Free [NBP3-18136] - Immunohistochemical analysis of paraffin-embedded human mouse tissue using anti-NCOA4 antibody (NBP3-18136) at 1 ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/NCOA4-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NBP3-18136-img0005.jpg)
![Immunohistochemistry-Paraffin: NCOA4 Antibody - BSA Free [NBP3-18136] - Immunohistochemical analysis of paraffin-embedded rat tissue using anti-NCOA4 antibody (NBP3-18136) at 1 ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/NCOA4 Antibody - BSA Free-Immunohistochemistry-Paraffin-NBP3-18136-img0007.jpg)
![Western Blot: NCOA4 Antibody (1F11) [H00008031-M05] - Analysis of NCOA4 expression in Hela S3 NE (Cat # L013V3).](http://images.novusbio.com/fullsize/NCOA4-Antibody-1F11-Western-Blot-H00008031-M05-img0007.jpg "Western Blot: NCOA4 Antibody (1F11) [H00008031-M05] - Analysis of NCOA4 expression in Hela S3 NE (Cat # L013V3).")
![Immunocytochemistry/Immunofluorescence: NCOA4 Antibody (1F11) [H00008031-M05] - Analysis of monoclonal antibody to NCOA4 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/NCOA4-Antibody-1F11-Immunocytochemistry-Immunofluorescence-H00008031-M05-img0005.jpg)
![Immunohistochemistry-Paraffin: NCOA4 Antibody (1F11) [H00008031-M05] - Analysis of monoclonal antibody to NCOA4 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/NCOA4-Antibody-1F11-Immunohistochemistry-Paraffin-H00008031-M05-img0006.jpg)
![ELISA: NCOA4 Antibody (1F11) [H00008031-M05] - Detection limit for recombinant GST tagged NCOA4 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/NCOA4-Antibody-1F11-ELISA-H00008031-M05-img0004.jpg)
![Western Blot: NCOA4 Antibody (1B7) [H00008031-M04] - Analysis of NCOA4 expression in NIH/3T3 (Cat # L018V1).](http://images.novusbio.com/fullsize/NCOA4-Antibody-1B7-Western-Blot-H00008031-M04-img0005.jpg "Western Blot: NCOA4 Antibody (1B7) [H00008031-M04] - Analysis of NCOA4 expression in NIH/3T3 (Cat # L018V1).")
![Immunocytochemistry/Immunofluorescence: NCOA4 Antibody (1B7) [H00008031-M04] - Analysis of monoclonal antibody to NCOA4 on NIH/3T3 cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/NCOA4-Antibody-1B7-Immunocytochemistry-Immunofluorescence-H00008031-M04-img0004.jpg)
![ELISA: NCOA4 Antibody (1B7) [H00008031-M04] - Detection limit for recombinant GST tagged NCOA4 is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/NCOA4-Antibody-1B7-ELISA-H00008031-M04-img0003.jpg)
![Western Blot: NCOA4 Antibody (1E5) [H00008031-M03] - Analysis of NCOA4 expression in Hela S3 NE (Cat # L013V3).](http://images.novusbio.com/fullsize/NCOA4-Antibody-1E5-Western-Blot-H00008031-M03-img0005.jpg "Western Blot: NCOA4 Antibody (1E5) [H00008031-M03] - Analysis of NCOA4 expression in Hela S3 NE (Cat # L013V3).")
![Immunohistochemistry-Paraffin: NCOA4 Antibody (1E5) [H00008031-M03] - Analysis of monoclonal antibody to NCOA4 on formalin-fixed paraffin-embedded human pancreas. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/NCOA4-Antibody-1E5-Immunohistochemistry-Paraffin-H00008031-M03-img0004.jpg)
![ELISA: NCOA4 Antibody (1E5) [H00008031-M03] - Detection limit for recombinant GST tagged NCOA4 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/NCOA4-Antibody-1E5-ELISA-H00008031-M03-img0003.jpg)
![Immunohistochemistry: NCOA4 Antibody [NBP1-28681] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ARA70 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit .](http://images.novusbio.com/fullsize/NCOA4-Antibody-Immunohistochemistry-NBP1-28681-img0004.jpg "Immunohistochemistry: NCOA4 Antibody [NBP1-28681] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ARA70 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit .")
![Immunohistochemistry-Paraffin: NCOA4 Antibody [NBP1-28681] - FFPE section of human prostate carcinoma. Antibody used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit.](http://images.novusbio.com/fullsize/NCOA4-Antibody-Immunohistochemistry-Paraffin-NBP1-28681-img0003.jpg)
![Immunoprecipitation: NCOA4 Antibody [NBP1-28681] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ARA70 antibody NBP1-28681 used for IP at 10 ug/mg lysate. ARA70 was also immunoprecipitated by rabbit anti-ARA70 antibody NBP1-28720, which recognizes a downstream epitope. For blotting immunoprecipitated ARA70, NBP1-28720 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/NCOA4-Antibody-Immunoprecipitation-NBP1-28681-img0005.jpg)
![Western Blot: NCOA4 Antibody [NBP1-28720] - Detection of human ARA70 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ARA70 antibody NBP1-28720 used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/NCOA4-Antibody-Western-Blot-NBP1-28720-img0004.jpg "Western Blot: NCOA4 Antibody [NBP1-28720] - Detection of human ARA70 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ARA70 antibody NBP1-28720 used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: NCOA4 Antibody [NBP1-28720] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NCOA4 antibody NBP1-28720 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. NCOA4 was also immunoprecipitated by rabbit anti-NCOA4 antibody NBP1-28681, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).](http://images.novusbio.com/fullsize/NCOA4-Antibody-Western-Blot-NBP1-28720-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: NCOA4 Antibody (2H8) [H00008031-M01] - Analysis of monoclonal antibody to NCOA4 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/NCOA4-Antibody-2H8-Immunocytochemistry-Immunofluorescence-H00008031-M01-img0003.jpg "Immunocytochemistry/Immunofluorescence: NCOA4 Antibody (2H8) [H00008031-M01] - Analysis of monoclonal antibody to NCOA4 on HeLa cell. Antibody concentration 10 ug/ml.")
![Immunocytochemistry/Immunofluorescence: NCOA4 Antibody [NBP2-56237] - Staining of human cell line HeLa shows localization to nucleoli, cytosol & the Golgi apparatus.](http://images.novusbio.com/fullsize/NCOA4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56237-img0001.jpg "Immunocytochemistry/Immunofluorescence: NCOA4 Antibody [NBP2-56237] - Staining of human cell line HeLa shows localization to nucleoli, cytosol & the Golgi apparatus.")
![Immunohistochemistry: NCOA4 Antibody [NBP2-39048] - Staining of human lung shows strong cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/NCOA4-Antibody-Immunohistochemistry-NBP2-39048-img0001.jpg "Immunohistochemistry: NCOA4 Antibody [NBP2-39048] - Staining of human lung shows strong cytoplasmic positivity in macrophages.")
![Western Blot: NCOA4 Overexpression Lysate (Adult Normal) [NBL1-13518] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NCOA4.](http://images.novusbio.com/fullsize/NCOA4-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13518-img0002.jpg "Western Blot: NCOA4 Overexpression Lysate (Adult Normal) [NBL1-13518] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NCOA4.")