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![Western Blot: METTL1 Antibody [NBP2-99811] - Anti-METTL1 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lane B: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 31 kDa. Observed band size: 34 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/METTL1-Antibody-Western-Blot-NBP2-99811-img0002.jpg "Western Blot: METTL1 Antibody [NBP2-99811] - Anti-METTL1 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lane B: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 31 kDa. Observed band size: 34 kDa (We are unsure as to the identity of these extra bands).")
![Immunohistochemistry-Paraffin: METTL1 Antibody [NBP2-99811] - Immunochemical staining of human METTL1 in human bladder carcinoma with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated wit](http://images.novusbio.com/fullsize/METTL1-Antibody-Immunohistochemistry-Paraffin-NBP2-99811-img0003.jpg)
![Immunoprecipitation: METTL1 Antibody [NBP2-99811] - METTL1 was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. Lane B: 0.5 mg 293T Whole Cell Lysate0.5 ul anti-METTL1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-METTL1 rabbit polyclonal antibody, at 1:1000 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 34 kDa. Observed band size: 34 kDa](http://images.novusbio.com/fullsize/METTL1-Antibody-Immunoprecipitation-NBP2-99811-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: METTL1 Antibody [NBP1-88386] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/METTL1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88386-img0006.jpg "Immunocytochemistry/Immunofluorescence: METTL1 Antibody [NBP1-88386] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.")
![Immunohistochemistry-Paraffin: METTL1 Antibody [NBP1-88386] - Staining of human urinary bladder shows strong nuclear positivity in urothelial cells.](http://images.novusbio.com/fullsize/METTL1-Antibody-Immunohistochemistry-Paraffin-NBP1-88386-img0002.jpg)
![Western Blot: METTL1 Antibody [NBP2-58787] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize/METTL1-Antibody-Western-Blot-NBP2-58787-img0002.jpg "Western Blot: METTL1 Antibody [NBP2-58787] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.")
![Immunocytochemistry/Immunofluorescence: METTL1 Antibody [NBP2-58787] - Staining of human cell line A-431 shows localization to nucleus.](http://images.novusbio.com/fullsize/METTL1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58787-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: METTL1 Antibody (HL2041) - Azide and BSA Free [NBP3-25561] - METTL1 antibody [HL2041] detects METTL1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: RMS-13 cells were fixed in ice-cold MeOH for 5 min. Green: METTL1 stained by METTL1 antibody [HL2041] (NBP3-25561) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25561_rabbit-mettl1-mab-hl2041-azide-and-bsa-free-2120249342721.jpg "Immunocytochemistry/Immunofluorescence: METTL1 Antibody (HL2041) - Azide and BSA Free [NBP3-25561] - METTL1 antibody [HL2041] detects METTL1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: RMS-13 cells were fixed in ice-cold MeOH for 5 min. Green: METTL1 stained by METTL1 antibody [HL2041] (NBP3-25561) diluted at 1:500. Blue: Fluoroshield with DAPI .")
![<b>Orthogonal Strategies Validation. </b>Western Blot: METTL1 Antibody (HL2041) - Azide and BSA Free [NBP3-25561] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with METTL1 antibody [HL2041] (NBP3-25561) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25561_rabbit-mettl1-mab-hl2041-azide-and-bsa-free-2120249393577.jpg)
![Western Blot: METTL1 Antibody (9C2F2) [NBP3-06651] - NBP3-06651 at 1:500 dilution. Lane A: 293T Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. Developed using the Odyssey technique and performed under reducing conditions. A band appears at an observed molecular weight of 31 kDa.](http://images.novusbio.com/fullsize/METTL1-Antibody-9C2F2-Western-Blot-NBP3-06651-img0001.jpg "Western Blot: METTL1 Antibody (9C2F2) [NBP3-06651] - NBP3-06651 at 1:500 dilution. Lane A: 293T Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. Developed using the Odyssey technique and performed under reducing conditions. A band appears at an observed molecular weight of 31 kDa.")
![Western Blot: METTL1 Antibody (9C2F2) [NBP3-06651] - METTL1 was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate. Lane B:0.5 mg Jurkat Whole Cell Lysate. 2 uL NBP3-06651 and 15 ul of 50 % Protein G agarose.Primary antibody: NBP3-06651 at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution. Developed using the odssey technique and performed under reducing conditions. A band appears at an observed molecular weight of 34 kDa.](http://images.novusbio.com/fullsize/METTL1-Antibody-9C2F2-Western-Blot-NBP3-06651-img0002.jpg)
![Western Blot: METTL1 Antibody [NBP2-22316] - Whole cell lysate (50 ug) from 293T, HeLa, Jurkat, and mouse TCMK-1 cells. NBP2-22316 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/METTL1-Antibody-Western-Blot-NBP2-22316-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: METTL1 Antibody [NBP2-22316] - Whole cell lysate (50 ug) from 293T, HeLa, Jurkat, and mouse TCMK-1 cells. NBP2-22316 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Western Blot: METTL1 Antibody [NBP1-54928] - HepG2 cell lysate, concentration 2.5 ug/ml.](http://images.novusbio.com/fullsize/METTL1-Antibody-Western-Blot-NBP1-54928-img0002.jpg "Western Blot: METTL1 Antibody [NBP1-54928] - HepG2 cell lysate, concentration 2.5 ug/ml.")
![Western Blot: METTL1 Overexpression Lysate (Adult Normal) [NBP2-07758] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for METTL1.](http://images.novusbio.com/fullsize/METTL1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-07758-img0001.jpg "Western Blot: METTL1 Overexpression Lysate (Adult Normal) [NBP2-07758] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for METTL1.")
![Western Blot: METTL1 Antibody [NBP3-04621] - Analysis of extracts of various cell lines, using METTL1 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit](http://images.novusbio.com/fullsize/METTL1-Antibody-Western-Blot-NBP3-04621-img0001.jpg "Western Blot: METTL1 Antibody [NBP3-04621] - Analysis of extracts of various cell lines, using METTL1 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit")
![SDS-Page: Recombinant Human METTL1 His Protein [NBP1-50953] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.](http://images.novusbio.com/fullsize/Recombinant Human METTL1 His Protein-SDS-Page-NBP1-50953-img0002.jpg "SDS-Page: Recombinant Human METTL1 His Protein [NBP1-50953] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.")
![Sandwich ELISA: METTL1 Antibody Pair [HRP] [NBP2-79646] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.](http://images.novusbio.com/fullsize/METTL1-Antibody-Pair-[HRP]-Sandwich-ELISA-NBP2-79646-img0001.jpg "Sandwich ELISA: METTL1 Antibody Pair [HRP] [NBP2-79646] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.")