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![Western Blot: TAK1 Antibody [NB100-56363] - Western blot analysis of TAK1 in cell lysates from NIH 3T3 cells using NB100-56363 at 1 ug/ml dilution.](http://images.novusbio.com/fullsize/TAK1-Antibody-Western-Blot-NB100-56363-img0003.jpg "Western Blot: TAK1 Antibody [NB100-56363] - Western blot analysis of TAK1 in cell lysates from NIH 3T3 cells using NB100-56363 at 1 ug/ml dilution.")
![Immunocytochemistry/Immunofluorescence: TAK1 Antibody [NB100-56363] - RH30 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-TAK1 Antibody NB100-56363 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/TAK1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56363-img0006.jpg)
![Immunohistochemistry-Paraffin: TAK1 Antibody [NB100-56363] - Analysis of a FFPE tissue section of human stomach using 1:200 dilution of TAK1 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/TAK1-Antibody-Immunohistochemistry-Paraffin-NB100-56363-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: TAK1 Antibody [NB100-56363] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-TAK1 Antibody NB100-56363 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/TAK1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56363-img0005.jpg)
![Western Blot: TAK1 Antibody [NBP3-13401] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TAK1 antibody (NBP3-13401) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/TAK1-Antibody-Western-Blot-NBP3-13401-img0004.jpg "Western Blot: TAK1 Antibody [NBP3-13401] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TAK1 antibody (NBP3-13401) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: TAK1 Antibody [NBP3-13401] - TAK1 antibody detects TAK1 protein at cell membrane and cytoplasm by immunofluorescent analysis. Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TAK1 stained by TAK1 antibody (NBP3-13401) diluted at 1:500. Blue: Fluoroshield with DAPI. Scale bar= 10 um.](http://images.novusbio.com/fullsize/TAK1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13401-img0005.jpg)
![Western Blot: TAK1 Antibody [NBP3-13401] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TAK1 antibody (NBP3-13401) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/TAK1-Antibody-Western-Blot-NBP3-13401-img0001.jpg)
![Western Blot: TAK1 Antibody [NBP3-13401] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TAK1 antibody (NBP3-13401) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/TAK1-Antibody-Western-Blot-NBP3-13401-img0002.jpg)
![Western Blot: TAK1 Antibody [NBP3-13401] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TAK1 antibody (NBP3-13401) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/TAK1-Antibody-Western-Blot-NBP3-13401-img0003.jpg)
![Western Blot: TAK1 Antibody [NBP1-76441] - Rat thymus cell lysate with TAK1 antibody at (A) 1, (B) 2, and (C) 4 ug/ml.](http://images.novusbio.com/fullsize/TAK1-Antibody-Western-Blot-NBP1-76441-img0001.jpg "Western Blot: TAK1 Antibody [NBP1-76441] - Rat thymus cell lysate with TAK1 antibody at (A) 1, (B) 2, and (C) 4 ug/ml.")
![Immunocytochemistry/Immunofluorescence: TAK1 Antibody [NBP1-76441] - Rat Thymus cells TAK1 antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/TAK1-Antibody-Immunofluorescence-NBP1-76441-img0002.jpg)
![Western Blot: TAK1 [p Thr187] Antibody [NBP3-16950] - Analysis of extracts of 293T cells, using TAK1 antibody (NBP3-16950) at 1:1000 dilution.293T cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/TAK1-[p-Thr187]-Antibody-Western-Blot-NBP3-16950-img0001.jpg "Western Blot: TAK1 [p Thr187] Antibody [NBP3-16950] - Analysis of extracts of 293T cells, using TAK1 antibody (NBP3-16950) at 1:1000 dilution.293T cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Western Blot: TAK1 [p Thr187] Antibody [NBP3-16950] - Analysis of extracts of NIH/3T3 cells, using TAK1 antibody (NBP3-16950) at 1:1000 dilution.NIH/3T3 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/TAK1-[p-Thr187]-Antibody-Western-Blot-NBP3-16950-img0002.jpg)
![Immunohistochemistry-Paraffin: TAK1 Antibody [NBP1-87819] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/TAK1-Antibody-Immunohistochemistry-Paraffin-NBP1-87819-img0005.jpg "Immunohistochemistry-Paraffin: TAK1 Antibody [NBP1-87819] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.")
![Western Blot: TAK1 [p Ser 439] Antibody (SR2176) [NBP3-21597] - Western blot analysis using NBP3-21597 in (1) HeLa treated with Calyculin A + IL-1 beta cell lysate; (2) Untreated.](http://images.novusbio.com/fullsize/nbp3-21597_rabbit-tak1-p-ser-439-mab-sr2176-6720239533717.jpg "Western Blot: TAK1 [p Ser 439] Antibody (SR2176) [NBP3-21597] - Western blot analysis using NBP3-21597 in (1) HeLa treated with Calyculin A + IL-1 beta cell lysate; (2) Untreated.")
![Western Blot: TAK1 Antibody (1W2B10) [NBP3-15650] - Western blot analysis of extracts of various cell lines, using TAK1 antibody (NBP3-15650) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/TAK1-Antibody-1W2B10-Western-Blot-NBP3-15650-img0001.jpg "Western Blot: TAK1 Antibody (1W2B10) [NBP3-15650] - Western blot analysis of extracts of various cell lines, using TAK1 antibody (NBP3-15650) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Western Blot: TAK1 [p Ser439] Antibody (2F4V1) [NBP3-16811] - Analysis of extracts of various cell lines, using (NBP3-16811) at 1:1000 dilution.C2C12 cells were treated by TGF-beta (10 ng/ml) at 37C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/TAK1-p-Ser439-Antibody-2F4V1-Western-Blot-NBP3-16811-img0001.jpg "Western Blot: TAK1 [p Ser439] Antibody (2F4V1) [NBP3-16811] - Analysis of extracts of various cell lines, using (NBP3-16811) at 1:1000 dilution.C2C12 cells were treated by TGF-beta (10 ng/ml) at 37C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")