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![Western blot analysis of whole brain tissue lysates using chicken pAb to microtubule associated protein 2 (MAP2), NB300-213, dilution 1:50,000 in green: [1] protein standard (red), [2] adult rat brain, [3] embryonic E20 rat brain, [4] adult mouse brain. Strong band observed at ~280 kDa mark corresponds to two major isoforms of MAP2 protein referred to as MAP2A and MAP2B. Smaller fragments of these isoforms are also detected if the antibody is used at higher concentrations.](http://images.novusbio.com/fullsize/MAP2-Antibody-Western-Blot-NB300-213-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: MAP2 Antibody (4H5) [NBP2-25156] - Mixed neuron and glia cultures stained with NBP2-25156 (green), and NB300-135 rabbit antibody to NF-H (red) and DNA (blue). NBP2-25156 reveals strong cytoplasmic staining for of dendrites and perikarya, which does not overlap with the NF-H antibody, which primarily binds to axons.](http://images.novusbio.com/fullsize/MAP2-Antibody-4H5-Immunocytochemistry-Immunofluorescence-NBP2-25156-img0003.jpg "Immunocytochemistry/Immunofluorescence: MAP2 Antibody (4H5) [NBP2-25156] - Mixed neuron and glia cultures stained with NBP2-25156 (green), and NB300-135 rabbit antibody to NF-H (red) and DNA (blue). NBP2-25156 reveals strong cytoplasmic staining for of dendrites and perikarya, which does not overlap with the NF-H antibody, which primarily binds to axons.")
![Immunohistochemistry Free-Floating: MAP2 Antibody (4H5) [NBP2-25156] - Analysis of a rat hippocampus section stained with mouse mAb to MAP2, NBP2-25156, dilution 1:2,000 in green, and costained with rabbit pAb to FOX3/NeuN, dilution 1:2,000 in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The NBP2-25156 antibody labels MAP2 protein in the perikarya and dendrites of most neurons while the FOX3/NeuN antibody selectively stains nuclei and proximal soma of neuronal cells.](http://images.novusbio.com/fullsize/MAP2-Antibody-4H5-Immunocytochemistry-Immunofluorescence-NBP2-25156-img0005.jpg)
![Western Blot: MAP2 Antibody (4H5) [NBP2-25156] - Analysis of tissue and cell lysates using mouse mAb to MAP2, NBP2-25156, dilution 1:10,000 in green: [1] protein standard (red), [2] rat brain, [3] mouse brain, and [4] embryonic rat cortical neuron-glial cell lysate. A band at about 280 kDa corresponds to the MAP2A and MAP2B proteins.](http://images.novusbio.com/fullsize/MAP2-Antibody-4H5-Western-Blot-NBP2-25156-img0006.jpg)
![Immunohistochemistry-Paraffin: MAP2 Antibody (4H5) [NBP2-25156] - Analysis of MAP2 in a 1% PFA fixed mouse brain section using anti-MAP2 (red) and NeuN (green) antibodies. Image from verified customer review.](http://images.novusbio.com/fullsize/MAP2-Antibody-4H5-Immunohistochemistry-Paraffin-NBP2-25156-img0004.jpg)
![Immunohistochemistry-Paraffin: MAP2 Antibody (4H5) [NBP2-25156] - Staining in mouse brain. Image from a verified customer review.](http://images.novusbio.com/fullsize/MAP2-Antibody-4H5-Immunohistochemistry-Paraffin-NBP2-25156-img0008.jpg)
![Immunohistochemistry-Paraffin: MAP2 Antibody (4H5) [NBP2-25156] - Staining of a 1% PFA fixed mouse cortical region section with anti-MAP 2 (dilution 1:300; Alexa 568 red). Image from a verified customer review.](http://images.novusbio.com/fullsize/MAP2-Antibody-4H5-Immunohistochemistry-Paraffin-NBP2-25156-img0007.jpg)
![Immunohistochemistry Free-Floating: MAP2 Antibody (5H11) [NBP1-92711] - Analysis of rat hippocampus section stained with mouse mAb to MAP2, NBP1-92711, dilution 1:5,000 in green, and costained with rabbit pAb to alpha-internexin, dilution 1:2,000 in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The NBP1-92711 antibody labels MAP2 protein in the perikarya and dendrites of the most neurons, and the alpha-internexin antibody selectively stains axons and dendrites of neuronal cells.](http://images.novusbio.com/fullsize/MAP2-Antibody-5H11-Immunocytochemistry-Immunofluorescence-NBP1-92711-img0001.jpg "Immunohistochemistry Free-Floating: MAP2 Antibody (5H11) [NBP1-92711] - Analysis of rat hippocampus section stained with mouse mAb to MAP2, NBP1-92711, dilution 1:5,000 in green, and costained with rabbit pAb to alpha-internexin, dilution 1:2,000 in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The NBP1-92711 antibody labels MAP2 protein in the perikarya and dendrites of the most neurons, and the alpha-internexin antibody selectively stains axons and dendrites of neuronal cells.")
![Western Blot: MAP2 Antibody (5H11) [NBP1-92711] - Analysis of different tissue lysates using mouse mAb to MAP2A/B (5H11), NBP1-92711, dilution 1:10,000 in green: [1] protein standard (red), [2] adult rat whole brain, [2] embryonic (E20) rat brain, [4] adult rat spinal cord, and [5] adult mouse brain lysate. A band observed at about 280 kDa corresponds to full length MAP2a and MAP2B protein. MAP2A/B is expressed heavily in brain particularly in cortical regions, but is a more minor component of spinal cord. Note that the epitope for this antibody in within the projection domain, and so the antibody does not bind to the lower molecular weight MAP2C and MAP2D isoforms which lack this region.](http://images.novusbio.com/fullsize/MAP2-Antibody-5H11-Western-Blot-NBP1-92711-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: MAP2 Antibody (5H11) [NBP1-92711] - Single cultured astrocytes stained with MAP2 (red) and Myelin (green). Image from a verified customer review.](http://images.novusbio.com/fullsize/MAP2-Antibody-5H11-Immunocytochemistry-Immunofluorescence-NBP1-92711-img0003.jpg)
![Western Blot: MAP2 Antibody (CL5420) [NBP2-61417] - Analysis in human cell line SH-SY5Y. Observed molecular weight 89 and 280 kDA.](http://images.novusbio.com/fullsize/MAP2-Antibody-CL5420-Western-Blot-NBP2-61417-img0007.jpg "Western Blot: MAP2 Antibody (CL5420) [NBP2-61417] - Analysis in human cell line SH-SY5Y. Observed molecular weight 89 and 280 kDA.")
![Immunocytochemistry/Immunofluorescence: MAP2 Antibody (CL5420) [NBP2-61417] - Staining of SH-SY5Y cells using the Anti-MAP2 monoclonal antibody, showing specific staining in the cytosol and in microtubules in green. Microtubule and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/MAP2-Antibody-CL5420-Immunocytochemistry-Immunofluorescence-NBP2-61417-img0002.jpg)
![Immunohistochemistry-Paraffin: MAP2 Antibody (CL5420) [NBP2-61417] - Staining of human tonsil shows absence of positivity in lymphoid cells as expected (negative control).](http://images.novusbio.com/fullsize/MAP2-Antibody-CL5420-Immunohistochemistry-Paraffin-NBP2-61417-img0006.jpg)
![Immunohistochemistry-Paraffin: MAP2 Antibody (CL5420) [NBP2-61417] - Staining of human cerebral cortex shows cytoplasmic immunoreactivity in neurons, as well as positivity in neuropil.](http://images.novusbio.com/fullsize/MAP2-Antibody-CL5420-Immunohistochemistry-Paraffin-NBP2-61417-img0003.jpg)
![Immunohistochemistry-Paraffin: MAP2 Antibody (CL5420) [NBP2-61417] - Staining of human cerebellum shows positivity in a subset of neurons.](http://images.novusbio.com/fullsize/MAP2-Antibody-CL5420-Immunohistochemistry-Paraffin-NBP2-61417-img0004.jpg)
![Immunohistochemistry-Paraffin: MAP2 Antibody (CL5420) [NBP2-61417] - Staining of human pancreas shows cytoplasmic immunoreactivity in pancreatic islets.](http://images.novusbio.com/fullsize/MAP2-Antibody-CL5420-Immunohistochemistry-Paraffin-NBP2-61417-img0005.jpg)
![Western Blot: MAP2 Antibody (HM-2) [NB120-11267] - Rat brain extract (adult and newborn) was separated on SDS-PAGE and probed with Monoclonal PA To MAP2 (MO) CL:HM-2 . The antibody was developed with AlkPhos APA Mouse Fab ads HIgG and a NBTBCIP substrate. Observed molecular weight is 280 kDA.](http://images.novusbio.com/fullsize/MAP2-Antibody-HM-2-Western-Blot-NB120-11267-img0003.jpg "Western Blot: MAP2 Antibody (HM-2) [NB120-11267] - Rat brain extract (adult and newborn) was separated on SDS-PAGE and probed with Monoclonal PA To MAP2 (MO) CL:HM-2 . The antibody was developed with AlkPhos APA Mouse Fab ads HIgG and a NBTBCIP substrate. Observed molecular weight is 280 kDA.")
![Immunocytochemistry/Immunofluorescence: MAP2 Antibody (HM-2) [NB120-11267] - B35 cells.](http://images.novusbio.com/fullsize/MAP2-Antibody-HM-2-Immunocytochemistry-Immunofluorescence-NB120-11267-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: MAP2 Antibody (2C4) [NBP2-50032] - Mixed neuron and glia cultures stained with NBP2-50032 (red) and chicken antibody to Vimentin (green) and DNA (blue). NBP2-50032 reveals strong cytoplasmic staining of dendrites and perikarya of neuronal cells, while Vimentin was visualized in astrocytes and fibroblasts.](http://images.novusbio.com/fullsize/MAP2-Antibody-2C4-Immunocytochemistry-Immunofluorescence-NBP2-50032-img0004.jpg "Immunocytochemistry/Immunofluorescence: MAP2 Antibody (2C4) [NBP2-50032] - Mixed neuron and glia cultures stained with NBP2-50032 (red) and chicken antibody to Vimentin (green) and DNA (blue). NBP2-50032 reveals strong cytoplasmic staining of dendrites and perikarya of neuronal cells, while Vimentin was visualized in astrocytes and fibroblasts.")
![Immunocytochemistry/Immunofluorescence: MAP2 Antibody (2C4) [NBP2-50032] - Analysis of adult rat hippocampus section stained with mouse mAb to MAP2A/B/C/D, NBP2-50032, dilution 1:5,000 in green, and chicken pAb to FOX2, dilution 1:2,000, in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 uM, and free-floating sections were stained with above antibodies. NBP2-50032 antibody labels all isotypes of the MAP2 protein which are found in the perikarya and dendrites of neurons. The FOX2 antibody stains the nuclei of a subset of neuronal cells.](http://images.novusbio.com/fullsize/MAP2-Antibody-2C4-Immunocytochemistry-Immunofluorescence-NBP2-50032-img0006.jpg)
![Western Blot: MAP2 Antibody (2C4) [NBP2-50032] - Analysis of tissue and cell lysates using mouse mAb to MAP2C/D, NBP2-50032, dilution 1:5,000 in green, and chicken pAb to Vimentin, dilution 1:5,000 in red. [1] protein standard (red), [2] rat whole brain lysate, [3] HeLa, [4] SH-SY5Y, [5] HEK293, and [6] NIH/3T3 cell lysates. A band at about 280 kDa corresponds to full length intact MAP2A/2B isotypes. The bands that appear at about 70kDa represent MAP2C/D isotypes. Multiple bands in between are likely to be in vivo fragments of MAP2A/B. Only the SH-SY5Y cells, which have neuronal properties express MAP2 protein. Vimentin antibody binds to vimentin protein showing a single band at ~50 kDa, which is present in all preparations.](http://images.novusbio.com/fullsize/MAP2-Antibody-2C4-Western-Blot-NBP2-50032-img0005.jpg)
![Western Blot: MAP2 Antibody [NBP3-05552] - Western blot analysis of whole brain lysates using MAP2 Antibody at a dilution of 1:5,000 in green: [1] protein standard (red), [2] rat, and [3] mouse brain lysate. A band at about 280kDa corresponds to the MAP2A and MAP2B proteins.](http://images.novusbio.com/fullsize/MAP2-Antibody-Western-Blot-NBP3-05552-img0002.jpg "Western Blot: MAP2 Antibody [NBP3-05552] - Western blot analysis of whole brain lysates using MAP2 Antibody at a dilution of 1:5,000 in green: [1] protein standard (red), [2] rat, and [3] mouse brain lysate. A band at about 280kDa corresponds to the MAP2A and MAP2B proteins.")
![Immunocytochemistry/Immunofluorescence: MAP2 Antibody [NBP3-05552] - Immunofluorescent analysis of rat brain stem section stained with goat pAb to MAP2 at a dilution of 1:2,000 in red, and costained with mouse mAb to MBP at a dilution of 1:5,000, in green. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. MAP2 Antibody labels MAP2 protein in the perikarya and dendrites of the most neurons, notably motorneurons in the brain stem, and the MBP antibody stains myelin sheath around axons.](http://images.novusbio.com/fullsize/MAP2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05552-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: MAP2 Antibody (HL1655) - Azide and BSA Free [NBP3-25554] - MAP2 antibody [HL1655] detects MAP2 protein in dendrite/axon, by immunofluorescent analysis. Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MAP2 stained by MAP2 antibody [HL1655] (NBP3-25554) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25554_rabbit-map2-mab-hl1655-azide-and-bsa-free-2120249403852.jpg "Immunocytochemistry/Immunofluorescence: MAP2 Antibody (HL1655) - Azide and BSA Free [NBP3-25554] - MAP2 antibody [HL1655] detects MAP2 protein in dendrite/axon, by immunofluorescent analysis. Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MAP2 stained by MAP2 antibody [HL1655] (NBP3-25554) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI .")
![Immunohistochemistry-Paraffin: MAP2 Antibody (HL1655) - Azide and BSA Free [NBP3-25554] - MAP2 antibody [HL1655] detects MAP2 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. MAP2 stained by MAP2 antibody [HL1655] (NBP3-25554) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25554_rabbit-map2-mab-hl1655-azide-and-bsa-free-2120249362750.jpg)
![Immunohistochemistry-Paraffin: MAP2 Antibody (HL1655) - Azide and BSA Free [NBP3-25554] - MAP2 antibody [HL1655] detects MAP2 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. MAP2 stained by MAP2 antibody [HL1655] (NBP3-25554) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25554_rabbit-map2-mab-hl1655-azide-and-bsa-free-212024939118.jpg)
![Immunohistochemistry-Paraffin: MAP2 Antibody (HL1655) - Azide and BSA Free [NBP3-25554] - MAP2 antibody [HL1655] detects MAP2 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. Green: MAP2 stained by MAP2 antibody [HL1655] (NBP3-25554) diluted at 1:100. Red: beta Tubulin 3/ Tuj1, a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25554_rabbit-map2-mab-hl1655-azide-and-bsa-free-2120249351398.jpg)
![Western Blot: MAP2 Antibody (5B7) [NBP2-37734] - Western blot analysis using MAP2 mAb against human MAP2 recombinant protein. (Observed MW is 36.1 kDa)](http://images.novusbio.com/fullsize/MAP2-Antibody-5B7-Western-Blot-NBP2-37734-img0002.jpg "Western Blot: MAP2 Antibody (5B7) [NBP2-37734] - Western blot analysis using MAP2 mAb against human MAP2 recombinant protein. (Observed MW is 36.1 kDa)")
![Flow Cytometry: MAP2 Antibody (5B7) [NBP2-37734] - Flow cytometric analysis of HeLa cells using MAP2 Antibody (5B7) (green) and negative control (red).](http://images.novusbio.com/fullsize/MAP2-Antibody-5B7-Flow-Cytometry-NBP2-37734-img0003.jpg)
![ELISA: MAP2 Antibody (5B7) [NBP2-37734] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/MAP2-Antibody-5B7-ELISA-NBP2-37734-img0001.jpg)
![Immunohistochemistry-Paraffin: MAP2 Antibody (HL1656) - Azide and BSA Free [NBP3-25555] - MAP2 antibody [HL1656] detects MAP2 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. MAP2 stained by MAP2 antibody [HL1656] (NBP3-25555) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25555_rabbit-map2-mab-hl1656-azide-and-bsa-free-212024939165.jpg "Immunohistochemistry-Paraffin: MAP2 Antibody (HL1656) - Azide and BSA Free [NBP3-25555] - MAP2 antibody [HL1656] detects MAP2 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. MAP2 stained by MAP2 antibody [HL1656] (NBP3-25555) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunocytochemistry/Immunofluorescence: MAP2 Antibody (HL1656) - Azide and BSA Free [NBP3-25555] - MAP2 antibody [HL1656] detects MAP2 protein in dendrites, but not in axons, by immunofluorescent analysis. Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MAP2 stained by MAP2 antibody [HL1656] (NBP3-25555) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25555_rabbit-map2-mab-hl1656-azide-and-bsa-free-212024941460.jpg)
![Immunohistochemistry-Paraffin: MAP2 Antibody (HL1656) - Azide and BSA Free [NBP3-25555] - MAP2 antibody [HL1656] detects MAP2 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. MAP2 stained by MAP2 antibody [HL1656] (NBP3-25555) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25555_rabbit-map2-mab-hl1656-azide-and-bsa-free-2120249413331.jpg)
![Immunocytochemistry/Immunofluorescence: MAP2 Antibody [NB100-98717] - Rat brain at 1:500 dilution using Sheep antibody to rat, mouse Mtap2 whole serum DAPI counterstained appearing in blue.](http://images.novusbio.com/fullsize/MAP2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-98717-img0003.jpg "Immunocytochemistry/Immunofluorescence: MAP2 Antibody [NB100-98717] - Rat brain at 1:500 dilution using Sheep antibody to rat, mouse Mtap2 whole serum DAPI counterstained appearing in blue.")