| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF244-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF774-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB244-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | 803-I2-200 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-66624-0.025mg | |||
![ICAM-2 WT and variants co-precipitated with alpha -actinin. A) IP/IB experiments with control cell lines demonstrate the expected association of ICAM-2, alpha -actinin and actin in lysates from SK-N-ASpIRES.ICAM-2 cells (labeled as WT), but not SK-N-ASpIRESneo cells (neo). Results for these control cell lines were published previously [13]. B) ICAM-2 glycosylation site variants associated simultaneously with alpha -actinin and actin. Immunoprecipitations (IP) were performed using whole cell lysates and a mouse monoclonal antibody to alpha -actinin (MAB1682, Millipore). Following protein separation by electrophoresis, immunoblots (IB) were performed with antibodies to alpha -actinin (sc-7454, Santa Cruz Biotech), ICAM-2 (AF244, R&D Systems), or actin (4968, Cell Signaling). The presence of ICAM-2 WT and variants in each preparation was confirmed by immunoblot analysis of input preparations (a representative blot is shown in Figure 2C) and also by immunoblot analysis of the proteins remaining in the supernatant following precipitation (not shown), to confirm the presence of sufficient/excess ICAM-2 protein or variant in each preparation used for immunoprecipitation experiments. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/23714211), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/af244_human-icam-2-cd102-affinity-purified-polyclonal-ab-8220249503222.jpg "ICAM-2 WT and variants co-precipitated with alpha -actinin. A) IP/IB experiments with control cell lines demonstrate the expected association of ICAM-2, alpha -actinin and actin in lysates from SK-N-ASpIRES.ICAM-2 cells (labeled as WT), but not SK-N-ASpIRESneo cells (neo). Results for these control cell lines were published previously [13]. B) ICAM-2 glycosylation site variants associated simultaneously with alpha -actinin and actin. Immunoprecipitations (IP) were performed using whole cell lysates and a mouse monoclonal antibody to alpha -actinin (MAB1682, Millipore). Following protein separation by electrophoresis, immunoblots (IB) were performed with antibodies to alpha -actinin (sc-7454, Santa Cruz Biotech), ICAM-2 (AF244, R&D Systems), or actin (4968, Cell Signaling). The presence of ICAM-2 WT and variants in each preparation was confirmed by immunoblot analysis of input preparations (a representative blot is shown in Figure 2C) and also by immunoblot analysis of the proteins remaining in the supernatant following precipitation (not shown), to confirm the presence of sufficient/excess ICAM-2 protein or variant in each preparation used for immunoprecipitation experiments. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/23714211), licensed under a CC-BY license. Not internally tested by R&D Systems.")
![ICAM-2 WT and variants co-precipitated with alpha -actinin. A) IP/IB experiments with control cell lines demonstrate the expected association of ICAM-2, alpha -actinin and actin in lysates from SK-N-ASpIRES.ICAM-2 cells (labeled as WT), but not SK-N-ASpIRESneo cells (neo). Results for these control cell lines were published previously [13]. B) ICAM-2 glycosylation site variants associated simultaneously with alpha -actinin and actin. Immunoprecipitations (IP) were performed using whole cell lysates and a mouse monoclonal antibody to alpha -actinin (MAB1682, Millipore). Following protein separation by electrophoresis, immunoblots (IB) were performed with antibodies to alpha -actinin (sc-7454, Santa Cruz Biotech), ICAM-2 (AF244, R&D Systems), or actin (4968, Cell Signaling). The presence of ICAM-2 WT and variants in each preparation was confirmed by immunoblot analysis of input preparations (a representative blot is shown in Figure 2C) and also by immunoblot analysis of the proteins remaining in the supernatant following precipitation (not shown), to confirm the presence of sufficient/excess ICAM-2 protein or variant in each preparation used for immunoprecipitation experiments. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/23714211), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/af244_human-icam-2-cd102-affinity-purified-polyclonal-ab-44202415425087.jpg)
![Western Blot: ICAM-2/CD102 Antibody [NBP1-31612] - Sample(30 ug whole cell lysate)A:HeLa S3 12% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/ICAM-2-CD102-Antibody-Western-Blot-NBP1-31612-img0005.jpg "Western Blot: ICAM-2/CD102 Antibody [NBP1-31612] - Sample(30 ug whole cell lysate)A:HeLa S3 12% SDS PAGE, antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: ICAM-2/CD102 Antibody [NBP1-31612] - Paraffin-embedded breast cancer, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/ICAM-2-CD102-Antibody-Immunohistochemistry-Paraffin-NBP1-31612-img0004.jpg)
![Western Blot: ICAM-2/CD102 Antibody [NBP2-16912] - Sample (30 ug of whole cell lysate) A: K562 7. 5% SDS PAGE gel, diluted at 1:3000.](http://images.novusbio.com/fullsize/ICAM-2-CD102-Antibody-Western-Blot-NBP2-16912-img0003.jpg "Western Blot: ICAM-2/CD102 Antibody [NBP2-16912] - Sample (30 ug of whole cell lysate) A: K562 7. 5% SDS PAGE gel, diluted at 1:3000.")
![Immunocytochemistry/Immunofluorescence: ICAM-2/CD102 Antibody [NBP2-16912] - K562 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ICAM2 protein stained by ICAM2 antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/ICAM-2-CD102-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16912-img0002.jpg)
![Western Blot: ICAM-2/CD102 Antibody [NBP2-32236] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-ICAM-2 antibody NBP2-32236 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/ICAM-2-CD102-Antibody-Western-Blot-NBP2-32236-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: ICAM-2/CD102 Antibody [NBP2-32236] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-ICAM-2 antibody NBP2-32236 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: ICAM2 Overexpression Lysate (Adult Normal) [NBP2-08994] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ICAM2.](http://images.novusbio.com/fullsize/ICAM2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-08994-img0001.jpg "Western Blot: ICAM2 Overexpression Lysate (Adult Normal) [NBP2-08994] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ICAM2.")
![Western Blot: ICAM2 Overexpression Lysate (Adult Normal) [NBP2-09325] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ICAM2.](http://images.novusbio.com/fullsize/ICAM2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09325-img0001.jpg "Western Blot: ICAM2 Overexpression Lysate (Adult Normal) [NBP2-09325] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ICAM2.")
![Western Blot: ICAM2 Overexpression Lysate (Adult Normal) [NBP2-09322] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ICAM2.](http://images.novusbio.com/fullsize/ICAM2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09322-img0001.jpg "Western Blot: ICAM2 Overexpression Lysate (Adult Normal) [NBP2-09322] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ICAM2.")
![Western Blot: ICAM2 Overexpression Lysate (Adult Normal) [NBP2-09324] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ICAM2.](http://images.novusbio.com/fullsize/ICAM2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09324-img0001.jpg "Western Blot: ICAM2 Overexpression Lysate (Adult Normal) [NBP2-09324] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ICAM2.")