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![Immunohistochemistry-Paraffin: Glucagon Antibody [NBP2-38329] - Analysis in human pancreas and skeletal muscle tissues. Corresponding GCG RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Glucagon-Antibody-Immunohistochemistry-Paraffin-NBP2-38329-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Glucagon Antibody [NBP2-38329] - Analysis in human pancreas and skeletal muscle tissues. Corresponding GCG RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Glucagon Antibody [NBP2-38329] - Staining of human colon, pancreas, skeletal muscle and small intestine using Anti-GCG antibody NBP2-38329 (A) shows similar protein distribution across tissues to independent antibody NBP2-38330 (B).](http://images.novusbio.com/fullsize/Glucagon-Antibody-Immunohistochemistry-Paraffin-NBP2-38329-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Glucagon Antibody [NBP2-38329] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Glucagon-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38329-img0009.jpg)
![Immunohistochemistry-Paraffin: Glucagon Antibody [NBP2-38329] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Glucagon-Antibody-Immunohistochemistry-Paraffin-NBP2-38329-img0014.jpg)
![Immunohistochemistry-Paraffin: Glucagon Antibody [NBP2-38329] - Staining of human pancreas shows strong cytoplasmic positivity in endocrine glandular cells.](http://images.novusbio.com/fullsize/Glucagon-Antibody-Immunohistochemistry-Paraffin-NBP2-38329-img0012.jpg)
![Immunohistochemistry-Paraffin: Glucagon Antibody [NBP2-38329] - Staining of human small intestine shows strong cytoplasmic positivity in enteroendocrine cells.](http://images.novusbio.com/fullsize/Glucagon-Antibody-Immunohistochemistry-Paraffin-NBP2-38329-img0013.jpg)
![Immunohistochemistry-Paraffin: Glucagon Antibody [NBP2-38329] - Staining of human colon shows moderate cytoplasmic positivity in enteroendocrine cells.](http://images.novusbio.com/fullsize/Glucagon-Antibody-Immunohistochemistry-Paraffin-NBP2-38329-img0015.jpg)
![Immunohistochemistry-Paraffin: Glucagon Antibody [NBP2-38330] - Analysis in human pancreas and skeletal muscle tissues. Corresponding GCG RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Glucagon-Antibody-Immunohistochemistry-Paraffin-NBP2-38330-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Glucagon Antibody [NBP2-38330] - Analysis in human pancreas and skeletal muscle tissues. Corresponding GCG RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Glucagon Antibody [NBP2-38330] - Staining of human colon, pancreas, skeletal muscle and small intestine using Anti-GCG antibody NBP2-38330 (A) shows similar protein distribution across tissues to independent antibody NBP2-38329 (B).](http://images.novusbio.com/fullsize/Glucagon-Antibody-Immunohistochemistry-Paraffin-NBP2-38330-img0008.jpg)
![Immunohistochemistry-Paraffin: Glucagon Antibody [NBP2-38330] - Staining of human pancreas shows strong cytoplasmic positivity in endocrine glandular cells.](http://images.novusbio.com/fullsize/Glucagon-Antibody-Immunohistochemistry-Paraffin-NBP2-38330-img0012.jpg)
![Immunohistochemistry-Paraffin: Glucagon Antibody [NBP2-38330] - Staining of human colon shows strong cytoplasmic positivity in enteroendocrine cells.](http://images.novusbio.com/fullsize/Glucagon-Antibody-Immunohistochemistry-Paraffin-NBP2-38330-img0010.jpg)
![Immunohistochemistry-Paraffin: Glucagon Antibody [NBP2-38330] - Staining of human small intestine shows strong cytoplasmic positivity in enteroendocrine cells.](http://images.novusbio.com/fullsize/Glucagon-Antibody-Immunohistochemistry-Paraffin-NBP2-38330-img0011.jpg)
![Immunohistochemistry-Paraffin: Glucagon Antibody [NBP2-38330] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Glucagon-Antibody-Immunohistochemistry-Paraffin-NBP2-38330-img0013.jpg)
![Western Blot: Glucagon Antibody [NBP2-16672] - Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/fullsize/Glucagon-Antibody-Western-Blot-NBP2-16672-img0002.jpg "Western Blot: Glucagon Antibody [NBP2-16672] - Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE gel, diluted at 1:500.")