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![Immunocytochemistry/Immunofluorescence: Fibrillarin Antibody (38F3) [NB300-269] - High magnification confocal image of HeLa cells stained with fibrillarin antibody, dilution 1:100 from concentrated tissue culture media (Green), and costained with chicken vimentin pAb (Red), dilution 1:1,000. Nuclear DNA is revealed with the DAPI stain (Blue). The fibrillarin antibody shows strong staining of nucleoli in the nucleus, while the vimentin antibody reveals cytoplasmic intermediate filaments.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-38F3-Immunocytochemistry-Immunofluorescence-NB300-269-img0008.jpg "Immunocytochemistry/Immunofluorescence: Fibrillarin Antibody (38F3) [NB300-269] - High magnification confocal image of HeLa cells stained with fibrillarin antibody, dilution 1:100 from concentrated tissue culture media (Green), and costained with chicken vimentin pAb (Red), dilution 1:1,000. Nuclear DNA is revealed with the DAPI stain (Blue). The fibrillarin antibody shows strong staining of nucleoli in the nucleus, while the vimentin antibody reveals cytoplasmic intermediate filaments.")
![Western Blot: Fibrillarin Antibody (38F3) [NB300-269] - Analysis of lysates of cell fractions probed with Fibrillarin antibody, dilution 1:500 (Green): [1] protein standard, [2] C6 cytosol, [3] C6 nuclear, [4] HEK293 cytosol, [5] HEK293 nuclear, [6] NIH-3T3 cytosol and [7] NIH-3T3 nuclear fractions. The band at 37kDa corresponds to the fibrillarin protein detected exclusively in the nuclear fractions.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-38F3-Western-Blot-NB300-269-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Fibrillarin Antibody (38F3) [NB300-269] - The nuclei of hth mutants have dispersed rDNA foci and ectopic nucleoli and produce more rRNA. A) Wild type nuclei stained with anti-Fibrillarin (red) and a specific probe that recognizes the rDNA genomic region (green). B) hth mutant nuclei stained as in A. The mutant nuclei exhibit an increased number of nucleoli with different sizes. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0120662), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-38F3-Immunocytochemistry-Immunofluorescence-NB300-269-img0012.jpg)
![Immunohistochemistry-Paraffin: Fibrillarin Antibody (38F3) [NB300-269] - IHC staining of Fibrillarin in human ovarian cancer using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-38F3-Immunohistochemistry-Paraffin-NB300-269-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Fibrillarin Antibody (38F3) [NB300-269] - Mouse embryonic fibroblast fibrillarin stained with fibrillarin antibody and Alexa-594 secondary antibody. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-38F3-Immunocytochemistry-Immunofluorescence-NB300-269-img0013.jpg)
![Flow Cytometry: Fibrillarin Antibody (38F3) [NB300-269] - Fibrillarin antibody was tested at 1:400 in HEK293 cells using an Alexa Fluor 488 secondary (shown in green) alongside unstained cells (shown in red).](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-38F3-Flow-Cytometry-NB300-269-img0004.jpg)
![Western Blot: Fibrillarin Antibody (38F3) [NB300-269] - Western blot analysis of Fibrillarin expression on yeast protein extracts using NB300-269.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-38F3-Western-Blot-NB300-269-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Fibrillarin Antibody (38F3) [NB300-269] - Fibrillarin immunofluorescence in fibroblasts. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-38F3-Immunocytochemistry-Immunofluorescence-NB300-269-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Fibrillarin Antibody (38F3) [NB300-269] - IF Confocal analysis of MCF7 cells using Fibrillarin antibody (NB300-269, 1:5). An Alexa Fluor 488-conjugated Goat to mouse IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-38F3-Immunocytochemistry-Immunofluorescence-NB300-269-img0006.jpg)
![Western Blot: Fibrillarin Antibody [NBP2-46881] - Western blot analysis of different cell lysates using chicken pAb to fibrillarin NBP2-46881, dilution 1:5,000, in green: [1] protein standard (red), [2] NIH-3T3, [3] HEK293, [4] HeLa, [5] SH-SY5Y, and [6] C6 cells. The single strong band at ~35kDa correspond to the fibrillarin protein.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-Western-Blot-NBP2-46881-img0002.jpg "Western Blot: Fibrillarin Antibody [NBP2-46881] - Western blot analysis of different cell lysates using chicken pAb to fibrillarin NBP2-46881, dilution 1:5,000, in green: [1] protein standard (red), [2] NIH-3T3, [3] HEK293, [4] HeLa, [5] SH-SY5Y, and [6] C6 cells. The single strong band at ~35kDa correspond to the fibrillarin protein.")
![Immunocytochemistry/Immunofluorescence: Fibrillarin Antibody [NBP2-46881] - Confocal immunofluorescent analysis of HeLa cells stained with chicken pAb to fibrillarin, NBP2-46881, dilution 1:10,000 in green and costained with mouse mAb to vimentin, 1:1,000, in red. The blue signal is DAPI staining of nuclear DNA. The fibrillarin antibody stains nucleoli while the vimentin antibody binds to cytoplasmic intermediate filaments.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-Immunofluorescence-NBP2-46881-img0001.jpg)
![Western Blot: Fibrillarin Antibody [NBP2-16492] - Fibrillarin antibody immunoprecipitates FBL protein in IP experiments. IP samples: HeLa nuclear extract. A. 35 ug HeLa nuclear extract. B. Control with 4 ug of preimmune Rabbit IgG. C. Immunoprecipitation of FBL protein by 4 ug Fibrillarin antibody. 10 % SDS-PAGE. The immunoprecipitated FBL protein was detected by Fibrillarin antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-Western-Blot-NBP2-16492-img0005.jpg "Western Blot: Fibrillarin Antibody [NBP2-16492] - Fibrillarin antibody immunoprecipitates FBL protein in IP experiments. IP samples: HeLa nuclear extract. A. 35 ug HeLa nuclear extract. B. Control with 4 ug of preimmune Rabbit IgG. C. Immunoprecipitation of FBL protein by 4 ug Fibrillarin antibody. 10 % SDS-PAGE. The immunoprecipitated FBL protein was detected by Fibrillarin antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Fibrillarin Antibody [NBP2-16492] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Fibrillarin antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16492-img0004.jpg)
![Western Blot: Fibrillarin Antibody [NBP2-16492] - 30 ug Rat whole cell lysate/extract 12 % SDS-PAGE Fibrillarin antibody dilution: 1:1000.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-Western-Blot-NBP2-16492-img0001.jpg)
![Western Blot: Fibrillarin Antibody [NBP2-16492] - Sample (30 ug of whole cell lysate) A: 293T B: H1299 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-Western-Blot-NBP2-16492-img0002.jpg)
![Western Blot: Fibrillarin Antibody [NBP2-16492] - Sample (30 ug of whole cell lysate) A:NIH-3T3 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-Western-Blot-NBP2-16492-img0003.jpg)
![Western Blot: Fibrillarin Antibody [NBP3-05533] - Western blot analysis of different tissue and cell line lysates using Fibrillarin Antibody at a dilution of 1:2,000, in green: [1] protein standard (red), [2] rat whole brain, [3] mouse whole brain, [4] NIH-3T3 cells,[5] HEK293, [6] HeLa, and [7] SH-SY5Y cells. Strong band at ~35kDa corresponds to Fibrillarin protein seen in all cell line lysates. A much weaker band is seen in tissue lysates since Fibrillarin is more heavily expressed in rapidly dividing cells. The blot simultaneously was probed with rabbit pAb to UCHL1 at a dilution of 1:3,000, in red, revealing the ~25kDa UCHL1 protein in lysates of tissues containing neurons or cells with neuronal properties.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-Western-Blot-NBP3-05533-img0002.jpg "Western Blot: Fibrillarin Antibody [NBP3-05533] - Western blot analysis of different tissue and cell line lysates using Fibrillarin Antibody at a dilution of 1:2,000, in green: [1] protein standard (red), [2] rat whole brain, [3] mouse whole brain, [4] NIH-3T3 cells,[5] HEK293, [6] HeLa, and [7] SH-SY5Y cells. Strong band at ~35kDa corresponds to Fibrillarin protein seen in all cell line lysates. A much weaker band is seen in tissue lysates since Fibrillarin is more heavily expressed in rapidly dividing cells. The blot simultaneously was probed with rabbit pAb to UCHL1 at a dilution of 1:3,000, in red, revealing the ~25kDa UCHL1 protein in lysates of tissues containing neurons or cells with neuronal properties.")
![Immunocytochemistry/Immunofluorescence: Fibrillarin Antibody [NBP3-05533] - Immunofluorescent analysis of (A) C6 rat glioma cells and (B) HEK293 human embryonic kidney cells stained with Fibrillarin Antibody at a dilution of 1:1,000 in red, in both cases costained with chicken pAb to vimentin at a dilution of 1:10,000 in green. The blue is DAPI staining of nuclear DNA. The Fibrillarin antibody detects Fibrillarin protein localized in nucleoli while the vimentin antibody produces strong staining of cytoplasmic intermediate filaments.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05533-img0001.jpg)
![Western Blot: Fibrillarin Antibody (9J5O2) [NBP3-15312] - Western blot analysis of extracts of various cell lines, using Fibrillarin Rabbit mAb (NBP3-15312) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-9J5O2-Western-Blot-NBP3-15312-img0004.jpg "Western Blot: Fibrillarin Antibody (9J5O2) [NBP3-15312] - Western blot analysis of extracts of various cell lines, using Fibrillarin Rabbit mAb (NBP3-15312) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: Fibrillarin Antibody (9J5O2) [NBP3-15312] - Immunofluorescence analysis of U-2 OS cells using Fibrillarin Rabbit mAb (NBP3-15312) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-9J5O2-Immunocytochemistry-Immunofluorescence-NBP3-15312-img0003.jpg)
![Western Blot: Fibrillarin Antibody (9J5O2) [NBP3-15312] - Western blot analysis of extracts of various cell lines, using Fibrillarin Rabbit mAb (NBP3-15312) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-9J5O2-Western-Blot-NBP3-15312-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Fibrillarin Antibody (9J5O2) [NBP3-15312] - Immunofluorescence analysis of C6 cells using Fibrillarin Rabbit mAb (NBP3-15312) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-9J5O2-Immunocytochemistry-Immunofluorescence-NBP3-15312-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Fibrillarin Antibody (9J5O2) [NBP3-15312] - Immunofluorescence analysis of NIH-3T3 cells using Fibrillarin Rabbit mAb (NBP3-15312) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-9J5O2-Immunocytochemistry-Immunofluorescence-NBP3-15312-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Fibrillarin Antibody (SR1545) [NBP3-21673] - Immunofluorescent analysis of Hela cells, using NBP3-21673.](http://images.novusbio.com/fullsize/nbp3-21673_rabbit-fibrillarin-mab-sr1545-57202315521843.jpg "Immunocytochemistry/Immunofluorescence: Fibrillarin Antibody (SR1545) [NBP3-21673] - Immunofluorescent analysis of Hela cells, using NBP3-21673.")
![Western Blot: Fibrillarin Antibody (SR1545) [NBP3-21673] - Western blot analysis using NBP3-21673 in HepG2 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21673_rabbit-fibrillarin-mab-sr1545-57202317215153.jpg)
![Immunohistochemistry: Fibrillarin Antibody (SR1545) [NBP3-21673] - Immunohistochemical analysis of paraffin-embedded human testis, using NBP3-21673.](http://images.novusbio.com/fullsize/nbp3-21673_rabbit-fibrillarin-mab-sr1545-5720231754866.jpg)
![Western Blot: Fibrillarin Antibody (28F2) [NBP1-05223] - Western Blot analysis of whole yeast protein extracts detect Fibrillarin/Nop1p. Molecular weight detected is 34.5 kDa.](http://images.novusbio.com/fullsize/p_105223_1.jpg "Western Blot: Fibrillarin Antibody (28F2) [NBP1-05223] - Western Blot analysis of whole yeast protein extracts detect Fibrillarin/Nop1p. Molecular weight detected is 34.5 kDa.")
![Western Blot: Fibrillarin Antibody [NBP2-26151] - NBP2-26151 (0.1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-Western-Blot-NBP2-26151-img0001.jpg "Western Blot: Fibrillarin Antibody [NBP2-26151] - NBP2-26151 (0.1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 | Lane 2: RT4 | Lane 3: U-251 MG | Lane 4: Human Plasma | Lane 5: Liver | Lane 6: Tonsil](http://images.novusbio.com/fullsize/nbp3-21414_rabbit-fibrillarin-pab-45202315524516.jpg "Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 | Lane 2: RT4 | Lane 3: U-251 MG | Lane 4: Human Plasma | Lane 5: Liver | Lane 6: Tonsil")
![Western Blot: Fibrillarin Antibody [NBP1-57272] - Jurkat cell lysate, Antibody Titration: 1.25ug/ml](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-Western-Blot-NBP1-57272-img0004.jpg "Western Blot: Fibrillarin Antibody [NBP1-57272] - Jurkat cell lysate, Antibody Titration: 1.25ug/ml")
![Immunohistochemistry-Paraffin: Fibrillarin Antibody [NBP1-57272] - Human Intestine Tissue, antibody concentration 4-8ug/ml. Cells with positive label: Epithelial cells of intestinal villus (indicated with arrows) 400X magnification.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-Immunohistochemistry-Paraffin-NBP1-57272-img0003.jpg)
![Western Blot: Fibrillarin Antibody [NBP2-26150] - NBP2-26150 (1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-Western-Blot-NBP2-26150-img0001.jpg "Western Blot: Fibrillarin Antibody [NBP2-26150] - NBP2-26150 (1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Fibrillarin Antibody [NBP2-58592] - Staining of human cell line U-251 MG shows localization to nucleus & nucleoli fibrillar center. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58592-img0001.jpg "Immunocytochemistry/Immunofluorescence: Fibrillarin Antibody [NBP2-58592] - Staining of human cell line U-251 MG shows localization to nucleus & nucleoli fibrillar center. Antibody staining is shown in green.")