| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3227-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61900-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47366-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61901-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76621-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-02995-20ul | |||
![Knockout Validated: 4EBP1 Antibody [NB200-157] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and 4EBP1 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Rabbit Anti-Human 4EBP1 Polyclonal Antibody (Catalog # NB200-157) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for 4EBP1 at approximately 17-20 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/4EBP1-Antibody-Knockout-Validated-NB200-157-img0011.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: 4EBP1 Antibody [NB200-157] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and 4EBP1 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Rabbit Anti-Human 4EBP1 Polyclonal Antibody (Catalog # NB200-157) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for 4EBP1 at approximately 17-20 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: 4EBP1 Antibody [NB200-157] - Whole cell lysate (100 ug) from RINm5F insulinoma exposed to high or low concentration of leucine. Antibody used at 0.25 ug/ml.](http://images.novusbio.com/fullsize/4EBP1-Antibody-Western-Blot-NB200-157-img0009.jpg)
![Immunohistochemistry: 4EBP1 Antibody [NB200-157] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-4EBP1 used at a dilution of 1:20,000 ( 0.05ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit.](http://images.novusbio.com/fullsize/4EBP1-Antibody-Immunohistochemistry-NB200-157-img0010.jpg)
![Western Blot: Rabbit Polyclonal 4EBP1 Antibody [NB200-157] - Whole cell lysates from MDA-MB-231, SUM159 and MCF-7 cells were loaded with 50 ug/lane. 10% SDS-PAGE. 4EBP1 Antibody (NB200-157) was used for primary antibody: 1:2000, 4C, overnight. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nb200-157_rabbit-polyclonal-4ebp1-antibody-western-blot-311202415623..png)
![Western Blot: 4EBP1 Antibody [NBP1-89368] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/4EBP1-Antibody-Western-Blot-NBP1-89368-img0006.jpg "Western Blot: 4EBP1 Antibody [NBP1-89368] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).")
![Immunohistochemistry-Paraffin: 4EBP1 Antibody [NBP1-89368] - Staining of human skin shows moderate cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/4EBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-89368-img0009.jpg)
![Western Blot: 4EBP1 Antibody [NBP1-89368] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-5](http://images.novusbio.com/fullsize/4EBP1-Antibody-Western-Blot-NBP1-89368-img0004.jpg)
![Immunohistochemistry-Paraffin: 4EBP1 Antibody [NBP1-89368] - Staining of human salivary gland shows strong cytoplasmic positivity in cells in serous acini.](http://images.novusbio.com/fullsize/4EBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-89368-img0007.jpg)
![Immunohistochemistry-Paraffin: 4EBP1 Antibody [NBP1-89368] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/4EBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-89368-img0008.jpg)
![Immunohistochemistry-Paraffin: 4EBP1 Antibody [NBP1-89368] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/4EBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-89368-img0010.jpg)
![Knockout Validated: 4EBP1 Antibody [NBP2-16307] - Wild-type (WT) and 4E-BP1 knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 antibody [N1C3]. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/4EBP1-Antibody-Western-Blot-NBP2-16307-img0004.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: 4EBP1 Antibody [NBP2-16307] - Wild-type (WT) and 4E-BP1 knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 antibody [N1C3]. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: 4EBP1 Antibody [NBP2-16307] - 4EBP1 antibody detects 4EBP1 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with 4EBP1 antibody diluted at a dilution of 1:2000.](http://images.novusbio.com/fullsize/4EBP1-Antibody-Western-Blot-NBP2-16307-img0006.jpg)
![Immunohistochemistry-Paraffin: 4EBP1 Antibody [NBP2-16307] - Analysis of paraffin-embedded RT2 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/eIF4EBP1-Antibody-Immunohistochemistry-Paraffin-NBP2-16307-img0003.jpg)
![Immunoprecipitation: 4EBP1 Antibody [NBP2-16307] - Immunoprecipitation of 4EBP1 protein from HCT116 whole cell extracts using 5 ug of 4EBP1 antibody or 4E-BP1 antibody. Western blot analysis was performed using 4EBP1 antibody.](http://images.novusbio.com/fullsize/4EBP1-Antibody-Immunoprecipitation-NBP2-16307-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: 4EBP1 Antibody (1E2E7) [NBP2-61900] - Analysis of Hela cells using Phospho-4E-BP1 (Ser65) mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/4EBP1-Antibody-1E2E7-Immunocytochemistry-Immunofluorescence-NBP2-61900-img0003.jpg "Immunocytochemistry/Immunofluorescence: 4EBP1 Antibody (1E2E7) [NBP2-61900] - Analysis of Hela cells using Phospho-4E-BP1 (Ser65) mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.")
![Immunohistochemistry: 4EBP1 Antibody (1E2E7) [NBP2-61900] - Analysis of paraffin-embedded bladder cancer tissues using Phospho-4E-BP1 (Ser65) mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/4EBP1-Antibody-1E2E7-Immunohistochemistry-NBP2-61900-img0004.jpg)
![Flow Cytometry: 4EBP1 Antibody (1E2E7) [NBP2-61900] - Analysis of Jurkat cells using Phospho-4E-BP1 (Ser65) mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/4EBP1-Antibody-1E2E7-Flow-Cytometry-NBP2-61900-img0002.jpg)
![Immunohistochemistry: 4EBP1 Antibody (1E2E7) [NBP2-61900] - Analysis of paraffin-embedded esophageal cancer tissues using Phospho-4E-BP1 (Ser65) mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/4EBP1-Antibody-1E2E7-Immunohistochemistry-NBP2-61900-img0005.jpg)
![ELISA: 4EBP1 Antibody (1E2E7) [NBP2-61900] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/4EBP1-Antibody-1E2E7-ELISA-NBP2-61900-img0001.jpg)
![Western Blot: 4EBP1 [p Thr46, p Thr37] Antibody [NBP3-13169] - Wild-type (WT) and 4EBP1 (phospho Thr37/Thr46) knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with 4EBP1 (phospho Thr37/Thr46) antibody (NBP3-13169) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/4EBP1-[p-Thr46--p-Thr37]-Antibody-Western-Blot-NBP3-13169-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: 4EBP1 [p Thr46, p Thr37] Antibody [NBP3-13169] - Wild-type (WT) and 4EBP1 (phospho Thr37/Thr46) knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with 4EBP1 (phospho Thr37/Thr46) antibody (NBP3-13169) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: 4EBP1 [p Thr46, p Thr37] Antibody [NBP3-13169] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with 4EBP1 (phospho Thr37/Thr46) antibody (NBP3-13169) diluted at 1:1000.](http://images.novusbio.com/fullsize/4EBP1-[p-Thr46--p-Thr37]-Antibody-Western-Blot-NBP3-13169-img0001.jpg)
![Western Blot: 4EBP1 [p Thr46, p Thr37] Antibody [NBP3-13169] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with 4EBP1 (phospho Thr37/Thr46) antibody (NBP3-13169) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/4EBP1-[p-Thr46--p-Thr37]-Antibody-Western-Blot-NBP3-13169-img0002.jpg)
![Western Blot: 4EBP1 [p Thr46, p Thr37] Antibody [NBP3-13169] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with 4EBP1 (phospho Thr37/Thr46) antibody (NBP3-13169) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/4EBP1-[p-Thr46--p-Thr37]-Antibody-Western-Blot-NBP3-13169-img0003.jpg)
![Western Blot: 4EBP1 [p Thr46, p Thr37] Antibody [NBP3-13169] -Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 (phospho Thr37/Thr46) antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/antibody/nbp3-13169_rabbit-4ebp1-p-thr37-p-thr46-pab-western-blot-273202412343..jpg)
![Western Blot: 4EBP1 [p Thr46, p Thr37] Antibody [NBP3-13170] - Wild-type (WT) and 4EBP1 (phospho Thr37/Thr46) knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with 4EBP1 (phospho Thr37/Thr46) antibody (NBP3-13170) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/4EBP1-[p-Thr46--p-Thr37]-Antibody-Western-Blot-NBP3-13170-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: 4EBP1 [p Thr46, p Thr37] Antibody [NBP3-13170] - Wild-type (WT) and 4EBP1 (phospho Thr37/Thr46) knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with 4EBP1 (phospho Thr37/Thr46) antibody (NBP3-13170) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: 4EBP1 [p Thr46, p Thr37] Antibody [NBP3-13170] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with 4EBP1 (phospho Thr37/Thr46) antibody (NBP3-13170) diluted at 1:1000.](http://images.novusbio.com/fullsize/4EBP1-[p-Thr46--p-Thr37]-Antibody-Western-Blot-NBP3-13170-img0001.jpg)
![Western Blot: 4EBP1 [p Thr46, p Thr37] Antibody [NBP3-13170] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with 4EBP1 (phospho Thr37/Thr46) antibody (NBP3-13170) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/4EBP1-[p-Thr46--p-Thr37]-Antibody-Western-Blot-NBP3-13170-img0002.jpg)
![Western Blot: 4EBP1 [p Thr46, p Thr37] Antibody [NBP3-13170] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with 4EBP1 (phospho Thr37/Thr46) antibody (NBP3-13170) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/4EBP1-[p-Thr46--p-Thr37]-Antibody-Western-Blot-NBP3-13170-img0003.jpg)
![Western Blot: 4EBP1 Antibody (11G12C11) [NBP1-47366] - Analysis using 4E-BP1 mouse mAb against truncated 4E-BP1 recombinant protein (1).](http://images.novusbio.com/fullsize/4EBP1-Antibody-11G12C11-Western-Blot-NBP1-47366-img0005.jpg "Western Blot: 4EBP1 Antibody (11G12C11) [NBP1-47366] - Analysis using 4E-BP1 mouse mAb against truncated 4E-BP1 recombinant protein (1).")
![Immunohistochemistry-Paraffin: 4EBP1 Antibody (11G12C11) [NBP1-47366] - Analysis of human pancreas carcinoma (A), esophagus carcinoma tissue (B) and ovary tumor tissue, showing cytoplasmic and membrane localization using 4E-BP1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/4EBP1-Antibody-11G12C11-Immunohistochemistry-Paraffin-NBP1-47366-img0004.jpg)
![Immunohistochemistry: 4EBP1 Antibody (2D1G11) [NBP2-61901] - Analysis of paraffin-embedded stomach cancer tissues using Phospho-4E-BP1 (Ser65) mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/4EBP1-Antibody-2D1G11-Immunohistochemistry-NBP2-61901-img0003.jpg "Immunohistochemistry: 4EBP1 Antibody (2D1G11) [NBP2-61901] - Analysis of paraffin-embedded stomach cancer tissues using Phospho-4E-BP1 (Ser65) mouse mAb with DAB staining.")
![Flow Cytometry: 4EBP1 Antibody (2D1G11) [NBP2-61901] - Analysis of Jurkat cells using Phospho-4E-BP1 (Ser65) mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/4EBP1-Antibody-2D1G11-Flow-Cytometry-NBP2-61901-img0002.jpg)
![Immunohistochemistry: 4EBP1 Antibody (2D1G11) [NBP2-61901] - Analysis of paraffin-embedded rectum cancer tissues using Phospho-4E-BP1 (Ser65) mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/4EBP1-Antibody-2D1G11-Immunohistochemistry-NBP2-61901-img0004.jpg)
![ELISA: 4EBP1 Antibody (2D1G11) [NBP2-61901] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/4EBP1-Antibody-2D1G11-ELISA-NBP2-61901-img0001.jpg)
![Western Blot: 4EBP1 Antibody [NBP1-76621] - eIF [NBP1-76621] - Analysis of eIF4EBP1 in human brain cell lysate with eIF4EBP1 antibody using NBP1-76621 at (A) 2.5, (B) 5 and (C) 10 ug/ml.](http://images.novusbio.com/fullsize/4EBP1-Antibody-Western-Blot-NBP1-76621-img0004.jpg "Western Blot: 4EBP1 Antibody [NBP1-76621] - eIF [NBP1-76621] - Analysis of eIF4EBP1 in human brain cell lysate with eIF4EBP1 antibody using NBP1-76621 at (A) 2.5, (B) 5 and (C) 10 ug/ml.")
![Immunocytochemistry/Immunofluorescence: 4EBP1 Antibody [NBP1-76621] - Staining of 4E-BP1 in 3T3 cells with 4E-BP1 antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/4EBP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76621-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: 4EBP1 Antibody [NBP1-76621] - 3T3 cells with eIF4EBP1 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/4EBP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76621-img0002.jpg)
![Western Blot: 4EBP1 Antibody [NBP3-02995] - Analysis of extracts of various cell lines, using eIF4EBP1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 300s.](http://images.novusbio.com/fullsize/4EBP1-Antibody-Western-Blot-NBP3-02995-img0004.jpg "Western Blot: 4EBP1 Antibody [NBP3-02995] - Analysis of extracts of various cell lines, using eIF4EBP1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 300s.")
![Immunocytochemistry/Immunofluorescence: 4EBP1 Antibody (007) [NBP2-89207] - Staining of Human 4EBP1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human 4EBP1 monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor (R) 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to nucleus and cytoplasm.](http://images.novusbio.com/fullsize/4EBP1-Antibody-007-Immunocytochemistry-Immunofluorescence-NBP2-89207-img0001.jpg "Immunocytochemistry/Immunofluorescence: 4EBP1 Antibody (007) [NBP2-89207] - Staining of Human 4EBP1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human 4EBP1 monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor (R) 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to nucleus and cytoplasm.")