| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76963-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77118SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1325-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-75511-0.025mg | |||
![Western Blot: Bim Antibody [NBP1-76963] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 ug of lysates per lane.Antibodies: BIM NBP1-76963, (0.5 ug/mL), BIM NBP1-77118, (5 ug/mL), beta-actin (1 ug/mL) and GAPDH (0.02 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Bim-Antibody-Western-Blot-NBP1-76963-img0012.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Bim Antibody [NBP1-76963] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 ug of lysates per lane.Antibodies: BIM NBP1-76963, (0.5 ug/mL), BIM NBP1-77118, (5 ug/mL), beta-actin (1 ug/mL) and GAPDH (0.02 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: Bim Antibody [NBP1-76963] - K562 cells with at 20 ug/mL.](http://images.novusbio.com/fullsize/Bim-Antibody-Immunofluorescence-NBP1-76963-img0007.jpg)
![Immunohistochemistry-Paraffin: Bim Antibody [NBP1-76963] - Human skin cancer cells with Bim antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/Bim-Antibody-Immunohistochemistry-Paraffin-NBP1-76963-img0001.jpg)
![Western Blot: Bim Antibody [NBP1-76963] - Western Blot Validation in Rat Myeloma Cell line:Loading: 15 ug of rat myeloma YB2/0 cell lysate per lane.Antibodies: BIM NBP1-76963, (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate.](http://images.novusbio.com/fullsize/Bim-Antibody-Western-Blot-NBP1-76963-img0008.jpg)
![Western Blot: Bim Antibody [NBP1-76963] - Western Blot Validation in HeLa Cells. Loading: 15 ug of lysate per lane. Antibodies: Bim NBP1-76963, (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate. Bim antibody can detect all three human isoforms, including EL, L and S isoforms.](http://images.novusbio.com/fullsize/Bim-Antibody-Western-Blot-NBP1-76963-img0009.jpg)
![Western Blot: Bim Antibody [NBP1-76963] - Western Blot Validation in Human Cell LinesLoading: 15 ug of lysates per lane.Antibodies: BIM NBP1-76963, (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Bim-Antibody-Western-Blot-NBP1-76963-img0010.jpg)
![Western Blot: Bim Antibody [NBP1-76963] - Western Blot Validation in Human TissueLoading: 15 ug of lysates per lane.Antibodies: BIM NBP1-76963, (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: Human thymusLane 2: Human colon](http://images.novusbio.com/fullsize/Bim-Antibody-Western-Blot-NBP1-76963-img0011.jpg)
![Western Blot: Bim Antibody [NBP2-53088] - Total protein from human K562, HeLa, A549, HepG2 cells and mouse Raw264.7 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Bim/BCL2L11 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Bim-Antibody-Western-Blot-NBP2-53088-img0001.jpg "Western Blot: Bim Antibody [NBP2-53088] - Total protein from human K562, HeLa, A549, HepG2 cells and mouse Raw264.7 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Bim/BCL2L11 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Bim Antibody [NBP2-53088] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Bim at a 1:50 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Bim-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-53088-img0003.jpg)
![Immunohistochemistry-Paraffin: Bim Antibody [NBP2-53088] - IHC analysis of a formalin fixed and paraffin embedded tissue section of human endometrial cancer xenograft using Bim antibody at 1:50 dilution. The signal was detected using HRP labeled secondary antibody and DAB staining method which followed counter-staining with hematoxylin. The antibody generated a specific cytoplasmic staining with punctate appearance, signifying Bim/Bcl2L11 presence on mitochondria.](http://images.novusbio.com/fullsize/Bim-Antibody-Immunohistochemistry-Paraffin-NBP2-53088-img0002.jpg)
![Western Blot: Bim Antibody (10B12) - BSA Free [NBP2-80103] - Using Bim, mAb (10B12) (Prod. No. NBP2-80103)](http://images.novusbio.com/fullsize/Bim-Antibody-10B12-BSA-Free-Western-Blot-NBP2-80103-img0001.jpg "Western Blot: Bim Antibody (10B12) - BSA Free [NBP2-80103] - Using Bim, mAb (10B12) (Prod. No. NBP2-80103)")
![Bim Antibody (10B12) - BSA Free [NBP2-80103] - Data in permeabilized and fixed transfected cells using Bim, mAb (10B12) (Prod. No. NBP2-80103)](http://images.novusbio.com/fullsize/Bim-Antibody-10B12-BSA-Free-NBP2-80103-img0002.jpg)
![Western Blot: Bim Antibody (3C5) [NBP2-80102] - Detecting EE mouse BimS, BimL, BimEL and EE human, BimSS, BimS, BimLS, BimL, BimELS, BimEL. Probing with an anti-Hsp-70 mAb was used as a loading control for all blots. B) Western blot using anti-Bim, mAb (3C5) detecting endogenous Bim expression in mouse tissues but not in Bim knockout mice. Bim protein was revealed in lysates from mouse tissues (5 x 106/lane) and detection by ECL. Probing with an anti-Hsp-70 mAb was used as a loading control.](http://images.novusbio.com/fullsize/Bim-Antibody-3C5-Western-Blot-NBP2-80102-img0002.jpg "Western Blot: Bim Antibody (3C5) [NBP2-80102] - Detecting EE mouse BimS, BimL, BimEL and EE human, BimSS, BimS, BimLS, BimL, BimELS, BimEL. Probing with an anti-Hsp-70 mAb was used as a loading control for all blots. B) Western blot using anti-Bim, mAb (3C5) detecting endogenous Bim expression in mouse tissues but not in Bim knockout mice. Bim protein was revealed in lysates from mouse tissues (5 x 106/lane) and detection by ECL. Probing with an anti-Hsp-70 mAb was used as a loading control.")
![Flow Cytometry: Bim Antibody (3C5) [NBP2-80102] - Data in permeabilized and fixed transfected cells using anti-Bim, mAb (3C5) anti-EE (positive control) or secondary antibody alone (negative control). Staining was visualised by FITC-conjugated goat anti-rat or anti-mouse IgG antibodies.](http://images.novusbio.com/fullsize/Bim-Antibody-3C5-Flow-Cytometry-NBP2-80102-img0001.jpg)
![Western Blot: Bim Antibody [NBP1-77118] - Western Blot Validation in Human TissueLoading: 15 ug of lysates per lane.Antibodies: BIM NBP1-77118, (5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: Human urinary bladderLane 2: Human pancreas](http://images.novusbio.com/fullsize/Bim-Antibody-Western-Blot-NBP1-77118-img0007.jpg "Western Blot: Bim Antibody [NBP1-77118] - Western Blot Validation in Human TissueLoading: 15 ug of lysates per lane.Antibodies: BIM NBP1-77118, (5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: Human urinary bladderLane 2: Human pancreas")
![Immunocytochemistry/Immunofluorescence: Bim Antibody [NBP1-77118] - K562 cells with Bim antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/Bim-Antibody-Immunocytochemistry-NBP1-77118-img0002.jpg)
![Western Blot: Bim Antibody [NBP1-77118] - Western Blot Validation in Human Cell LinesLoading: 15 ug of lysates per lane.Antibodies: BIM NBP1-77118, (5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Bim-Antibody-Western-Blot-NBP1-77118-img0005.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: Bim Antibody [NBP1-77118] - IAV via Protein Expression Profile in Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: BIM NBP1-76963 (0.5 ug/ml), BIM NBP1-77118 (5 ug/ml), beta-actin (1 ug/ml) and GAPDH (0.02 ug/ml), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Bim-Antibody-Western-Blot-NBP1-77118-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Bim Antibody [NBP1-77118] - K562 cells at 20 ug/mL.](http://images.novusbio.com/fullsize/Bim-Antibody-Immunofluorescence-NBP1-77118-img0004.jpg)
![Western Blot: Bim Antibody (1C2H4) [NBP1-75511] - Western blot analysis of 5 ng of Bim recombinant protein with Bim antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/Bim-Antibody-1C2H4-Western-Blot-NBP1-75511-img0003.jpg "Western Blot: Bim Antibody (1C2H4) [NBP1-75511] - Western blot analysis of 5 ng of Bim recombinant protein with Bim antibody at 1 ug/ml.")
![Immunocytochemistry/Immunofluorescence: Bim Antibody (1C2H4) [NBP1-75511] - Immunocytochemistry of Bim in K562 cells with Bim antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/Bim-Antibody-1C2H4-Immunocytochemistry-Immunofluorescence-NBP1-75511-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Bim Antibody (1C2H4) [NBP1-75511] - Immunofluorescence of Bim in K562 cells with Bim antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/Bim-Antibody-1C2H4-Immunocytochemistry-Immunofluorescence-NBP1-75511-img0004.jpg)
![Western Blot: Bim Antibody [NB100-1440] - (0.5ug/ml) of K562lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.](http://images.novusbio.com/fullsize/Bim-Antibody-Western-Blot-NB100-1440-img0002.jpg "Western Blot: Bim Antibody [NB100-1440] - (0.5ug/ml) of K562lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.")
![Western Blot: Bim Antibody (3R0T2) [NBP3-15867] - Western blot analysis of extracts of various cell lines, using Bim antibody (NBP3-15867) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Bim-Antibody-3R0T2-Western-Blot-NBP3-15867-img0001.jpg "Western Blot: Bim Antibody (3R0T2) [NBP3-15867] - Western blot analysis of extracts of various cell lines, using Bim antibody (NBP3-15867) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Western Blot: Bim Antibody (3R0T2) [NBP3-15867] - Western blot analysis of extracts of various cell lines, using Bim antibody (NBP3-15867) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Bim-Antibody-3R0T2-Western-Blot-NBP3-15867-img0002.jpg)
![Western Blot: Bim Overexpression Lysate (Adult Normal) [NBP2-10405] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Bim.](http://images.novusbio.com/fullsize/Bim-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10405-img0001.jpg "Western Blot: Bim Overexpression Lysate (Adult Normal) [NBP2-10405] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Bim.")
![ELISA: Human Bim ELISA Kit (Colorimetric) [NBP2-69950] - Samples were spiked with high concentrations of Human Bim and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-69950_human-bim-elisa-kit-colorimetric-132202416225278.jpg "ELISA: Human Bim ELISA Kit (Colorimetric) [NBP2-69950] - Samples were spiked with high concentrations of Human Bim and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Bim ELISA Kit (Colorimetric) [NBP2-69950] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-69950_human-bim-elisa-kit-colorimetric-432024903533.jpg)