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![Western Blot: actin-related protein 2/3 complex subunit 1B Antibody [NBP1-90114] - Analysis in human cell lines SK-MEL-30 and HEK293 using anti-ARPC1B antibody. Corresponding ARPC1B RNA-seq data are presented for the same cell lines. Loading control: anti-HDAC1.](http://images.novusbio.com/fullsize/actin-related-protein-2-3-complex-subunit-1B-Antibody-Western-Blot-NBP1-90114-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: actin-related protein 2/3 complex subunit 1B Antibody [NBP1-90114] - Analysis in human cell lines SK-MEL-30 and HEK293 using anti-ARPC1B antibody. Corresponding ARPC1B RNA-seq data are presented for the same cell lines. Loading control: anti-HDAC1.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: actin-related protein 2/3 complex subunit 1B Antibody [NBP1-90114] - Staining of human lymph node shows strong cytoplasmic positivity in germinal center cells.](http://images.novusbio.com/fullsize/actin-related-protein-2-3-complex-subunit-1B-Antibody-Immunohistochemistry-Paraffin-NBP1-90114-img0016.jpg)
![Western Blot: actin-related protein 2/3 complex subunit 1B Antibody [NBP1-90114] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/actin-related-protein-2-3-complex-subunit-1B-Antibody-Western-Blot-NBP1-90114-img0006.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: actin-related protein 2/3 complex subunit 1B Antibody [NBP1-90114] - Analysis in human lymph node and skeletal muscle tissues. Corresponding ARPC1B RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/actin-related-protein-2-3-complex-subunit-1B-Antibody-Immunohistochemistry-Paraffin-NBP1-90114-img0012.jpg)
![Immunohistochemistry-Paraffin: actin-related protein 2/3 complex subunit 1B Antibody [NBP1-90114] - Staining of human lung shows weak cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/actin-related-protein-2-3-complex-subunit-1B-Antibody-Immunohistochemistry-Paraffin-NBP1-90114-img0013.jpg)
![Immunohistochemistry-Paraffin: actin-related protein 2/3 complex subunit 1B Antibody [NBP1-90114] - Staining of human small intestine shows moderate cytoplasmic positivity in lymphoid cells.](http://images.novusbio.com/fullsize/actin-related-protein-2-3-complex-subunit-1B-Antibody-Immunohistochemistry-Paraffin-NBP1-90114-img0014.jpg)
![Immunohistochemistry-Paraffin: actin-related protein 2/3 complex subunit 1B Antibody [NBP1-90114] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/actin-related-protein-2-3-complex-subunit-1B-Antibody-Immunohistochemistry-Paraffin-NBP1-90114-img0015.jpg)
![Western Blot: actin-related protein 2/3 complex subunit 1B Antibody [NB300-898] - Staining (2 ug/ml) of Human Liver lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/actin-related-protein-2-3-complex-subunit-1B-Antibody-Western-Blot-NB300-898-img0004.jpg "Western Blot: actin-related protein 2/3 complex subunit 1B Antibody [NB300-898] - Staining (2 ug/ml) of Human Liver lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: actin-related protein 2/3 complex subunit 1B Antibody [NB300-898] - Staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/actin-related-protein-2-3-complex-subunit-1B-Antibody-Immunohistochemistry-Paraffin-NB300-898-img0001.jpg)
![Western Blot: actin-related protein 2/3 complex subunit 1B Antibody [NBP1-46219] - Detection of Human and Mouse Arpc1b by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Arpc1b antibody NBP1-46219 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/actin-related-protein-2-3-complex-subunit-1B-Antibody-Western-Blot-NBP1-46219-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: actin-related protein 2/3 complex subunit 1B Antibody [NBP1-46219] - Detection of Human and Mouse Arpc1b by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Arpc1b antibody NBP1-46219 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: actin-related protein 2/3 complex subunit 1B Antibody [NBP1-46219] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-Arpc1b antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. actin-related protein 2/3 complex subunit 1B was also immunoprecipitated by NBP1-46220, which recognizes a downstream epitope.](http://images.novusbio.com/fullsize/actin-related-protein-2-3-complex-subunit-1B-Antibody-Western-Blot-NBP1-46219-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: actin-related protein 2/3 complex subunit 1B Antibody [NBP1-46219] - Detection of human Arpc1b by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Arpc1b antibody NBP1-46219 (lot NBP1-46219-3) used for IP at 3 ug per reaction. Arpc1b was also immunoprecipitated by a previous lot of this antibody (lot NBP1-46219-2) and rabbit anti-Arpc1b antibody NBP1-46220. For blotting immunoprecipitated Arpc1b, A302-780A was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/actin-related-protein-2-3-complex-subunit-1B-Antibody-Immunoprecipitation-NBP1-46219-img0004.jpg)
![Western Blot: actin-related protein 2/3 complex subunit 1B Antibody [NBP2-15263] - Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize/actin-related-protein-2-3-complex-subunit-1B-Antibody-Western-Blot-NBP2-15263-img0001.jpg "Western Blot: actin-related protein 2/3 complex subunit 1B Antibody [NBP2-15263] - Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE, antibody diluted at 1:500.")
![Immunohistochemistry-Paraffin: actin-related protein 2/3 complex subunit 1B Antibody [NBP2-15263] - Paraffin-embedded human lung carcinoma. p41-ARCb antibody [N1], N-term dilution: 1:500](http://images.novusbio.com/fullsize/actin-related-protein-2-3-complex-subunit-1B-Antibody-Immunohistochemistry-Paraffin-NBP2-15263-img0002.jpg)
![Western Blot: actin-related protein 2/3 complex subunit 1B Antibody [NBP3-23370] - Western blot of rat A7r5 (lane 1), human Jurkat (lane 2), rat PC12 (lane 3), human PC3 (lane 4), mouse C2C12 (lane 5), and human A431 cells (lane 6). The blot was probed with rabbit polyclonal anti-Arpc1b (C-terminal region).](http://images.novusbio.com/fullsize/nbp3-23370_rabbit-actin-related-protein-2-3-complex-subunit-1b-pab-1992023199287.jpg "Western Blot: actin-related protein 2/3 complex subunit 1B Antibody [NBP3-23370] - Western blot of rat A7r5 (lane 1), human Jurkat (lane 2), rat PC12 (lane 3), human PC3 (lane 4), mouse C2C12 (lane 5), and human A431 cells (lane 6). The blot was probed with rabbit polyclonal anti-Arpc1b (C-terminal region).")
![Western Blot: actin-related protein 2/3 complex subunit 1B Antibody [NBP2-15262] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize/actin-related-protein-2-3-complex-subunit-1B-Antibody-Western-Blot-NBP2-15262-img0002.jpg "Western Blot: actin-related protein 2/3 complex subunit 1B Antibody [NBP2-15262] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE, antibody diluted at 1:500.")
![Immunocytochemistry/Immunofluorescence: actin-related protein 2/3 complex subunit 1B Antibody [NBP2-15262] - Analysis of methanol-fixed A431, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/actin-related-protein-2-3-complex-subunit-1B-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15262-img0001.jpg)
![Western Blot: actin-related protein 2/3 complex subunit 1B [p Thr21] Antibody [NBP3-23382] - Western blot analysis of Arpc1b phosphorylation in human A431 stimulated with calyculin A (100 nM) for 30 min (lanes 1 & 3). The blots were treated with lambda phosphatase to remove phosphorylation (lanes 2 & 4), then probed with anti-Arpc1b (C-terminal region) (lanes 1 & 2) or anti-Arpc1b (Thr-21) (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23382_rabbit-actin-related-protein-2-3-complex-subunit-1b-p-thr21-pab-199202312523618.jpg "Western Blot: actin-related protein 2/3 complex subunit 1B [p Thr21] Antibody [NBP3-23382] - Western blot analysis of Arpc1b phosphorylation in human A431 stimulated with calyculin A (100 nM) for 30 min (lanes 1 & 3). The blots were treated with lambda phosphatase to remove phosphorylation (lanes 2 & 4), then probed with anti-Arpc1b (C-terminal region) (lanes 1 & 2) or anti-Arpc1b (Thr-21) (lanes 3 & 4).")
![Western Blot: actin-related protein 2/3 complex subunit 1B Antibody [NBP1-46220] - Detection of Human and Mouse Arpc1b by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Arpc1b antibody NBP1-46220 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/actin-related-protein-2-3-complex-subunit-1B-Antibody-Western-Blot-NBP1-46220-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: actin-related protein 2/3 complex subunit 1B Antibody [NBP1-46220] - Detection of Human and Mouse Arpc1b by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Arpc1b antibody NBP1-46220 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunoprecipitation: actin-related protein 2/3 complex subunit 1B Antibody [NBP1-46220] - Detection of human Arpc1b by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Arpc1b antibody NBP1-46220 (lot NBP1-46220-3) used for IP at 6 ug per reaction. Arpc1b was also immunoprecipitated by a previous lot of this antibody (lot NBP1-46220-2) and rabbit anti-Arpc1b antibody NBP1-46219. For blotting immunoprecipitated Arpc1b, NBP1-46220 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/actin-related-protein-2-3-complex-subunit-1B-Antibody-Immunoprecipitation-NBP1-46220-img0004.jpg)
![Western Blot: actin-related protein 2/3 complex subunit 1B Antibody [NBP2-15264] - Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/actin-related-protein-2-3-complex-subunit-1B-Antibody-Western-Blot-NBP2-15264-img0001.jpg "Western Blot: actin-related protein 2/3 complex subunit 1B Antibody [NBP2-15264] - Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE; antibody diluted at 1:1000.")
![Western Blot: actin-related protein 2/3 complex subunit 1B Overexpression Lysate (Adult Normal) [NBL1-07726] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for actin-related protein 2/3 complex subunit 1B.](http://images.novusbio.com/fullsize/actin-related-protein-2-3-complex-subunit-1B-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07726-img0002.jpg "Western Blot: actin-related protein 2/3 complex subunit 1B Overexpression Lysate (Adult Normal) [NBL1-07726] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for actin-related protein 2/3 complex subunit 1B.")
![Western Blot: actin-related protein 2/3 complex subunit 1B Antibody [NBP2-99248] - Anti-actin-related protein 2/3 complex subunit 1B rabbit polyclonal antibody at 1:500 dilution. Lane A: A431 Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lane C: A549 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 41 kDa. Observed band size: 37 kDa](http://images.novusbio.com/fullsize/actin-related-protein-2-3-complex-subunit-1B-Antibody-Western-Blot-NBP2-99248-img0001.jpg "Western Blot: actin-related protein 2/3 complex subunit 1B Antibody [NBP2-99248] - Anti-actin-related protein 2/3 complex subunit 1B rabbit polyclonal antibody at 1:500 dilution. Lane A: A431 Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lane C: A549 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 41 kDa. Observed band size: 37 kDa")