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![Western Blot: A-RAF Antibody (2G9) [NBP2-46538] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARAF.](http://images.novusbio.com/fullsize/A-RAF-Antibody-2G9-Western-Blot-NBP2-46538-img0014.jpg "Western Blot: A-RAF Antibody (2G9) [NBP2-46538] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARAF.")
![Immunohistochemistry: A-RAF Antibody (2G9) [NBP2-46538] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/A-RAF-Antibody-2G9-Immunohistochemistry-NBP2-46538-img0013.jpg)
![Immunohistochemistry: A-RAF Antibody (2G9) [NBP2-46538] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/A-RAF-Antibody-2G9-Immunohistochemistry-NBP2-46538-img0001.jpg)
![Immunohistochemistry: A-RAF Antibody (2G9) [NBP2-46538] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/A-RAF-Antibody-2G9-Immunohistochemistry-NBP2-46538-img0002.jpg)
![Immunohistochemistry: A-RAF Antibody (2G9) [NBP2-46538] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/A-RAF-Antibody-2G9-Immunohistochemistry-NBP2-46538-img0003.jpg)
![Immunohistochemistry: A-RAF Antibody (2G9) [NBP2-46538] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/A-RAF-Antibody-2G9-Immunohistochemistry-NBP2-46538-img0004.jpg)
![Immunohistochemistry: A-RAF Antibody (2G9) [NBP2-46538] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/A-RAF-Antibody-2G9-Immunohistochemistry-NBP2-46538-img0005.jpg)
![Immunohistochemistry: A-RAF Antibody (2G9) [NBP2-46538] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/A-RAF-Antibody-2G9-Immunohistochemistry-NBP2-46538-img0006.jpg)
![Immunohistochemistry: A-RAF Antibody (2G9) [NBP2-46538] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/A-RAF-Antibody-2G9-Immunohistochemistry-NBP2-46538-img0007.jpg)
![Immunohistochemistry: A-RAF Antibody (2G9) [NBP2-46538] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/A-RAF-Antibody-2G9-Immunohistochemistry-NBP2-46538-img0008.jpg)
![Immunohistochemistry: A-RAF Antibody (2G9) [NBP2-46538] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/A-RAF-Antibody-2G9-Immunohistochemistry-NBP2-46538-img0009.jpg)
![Immunohistochemistry: A-RAF Antibody (2G9) [NBP2-46538] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/A-RAF-Antibody-2G9-Immunohistochemistry-NBP2-46538-img0010.jpg)
![Immunohistochemistry: A-RAF Antibody (2G9) [NBP2-46538] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/A-RAF-Antibody-2G9-Immunohistochemistry-NBP2-46538-img0011.jpg)
![Immunohistochemistry: A-RAF Antibody (2G9) [NBP2-46538] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/A-RAF-Antibody-2G9-Immunohistochemistry-NBP2-46538-img0012.jpg)
![Western Blot: A-RAF Antibody [NBP1-33067] - Sample (30 ug of whole cell lysate) A: Hela 7.5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/A-RAF-Antibody-Western-Blot-NBP1-33067-img0011.jpg "Western Blot: A-RAF Antibody [NBP1-33067] - Sample (30 ug of whole cell lysate) A: Hela 7.5% SDS PAGE; antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: A-RAF Antibody [NBP1-33067] - Analysis of paraformaldehyde-fixed A431, using A-RAF Antibody.](http://images.novusbio.com/fullsize/A-RAF-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33067-img0008.jpg)
![Immunohistochemistry-Paraffin: A-RAF Antibody [NBP1-33067] - Paraffin-embedded Cal27 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/A-RAF-Antibody-Immunohistochemistry-Paraffin-NBP1-33067-img0009.jpg)
![Western Blot: A-RAF Antibody [NBP1-33067] - Sample (30 ug of whole cell lysate) NIH-3T37C 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/A-RAF-Antibody-Western-Blot-NBP1-33067-img0010.jpg)
![Western Blot: A-RAF Antibody (3E2) [H00000369-M05] - Analysis of ARAF expression in transfected 293T cell line by ARAF monoclonal antibody (M05), clone 3E2. Lane 1: ARAF transfected lysatE (67.6 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/A-RAF-Antibody-3E2-Western-Blot-H00000369-M05-img0009.jpg "Western Blot: A-RAF Antibody (3E2) [H00000369-M05] - Analysis of ARAF expression in transfected 293T cell line by ARAF monoclonal antibody (M05), clone 3E2. Lane 1: ARAF transfected lysatE (67.6 KDa). Lane 2: Non-transfected lysate.")
![Western Blot: A-RAF Antibody (3E2) [H00000369-M05] - Analysis of ARAF expression in HeLa (Cat # L013V1).](http://images.novusbio.com/fullsize/A-RAF-Antibody-3E2-Western-Blot-H00000369-M05-img0008.jpg)
![ELISA: A-RAF Antibody (3E2) [H00000369-M05] - Detection limit for recombinant GST tagged ARAF is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/A-RAF-Antibody-3E2-ELISA-H00000369-M05-img0004.jpg)
![Proximity Ligation Assay: A-RAF Antibody (3E2) [H00000369-M05] - Analysis of protein-protein interactions between PKM2 and ARAF. HeLa cells were stained with anti-PKM2 rabbit purified polyclonal 1:1200 and anti-ARAF mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/A-RAF-Antibody-3E2-Proximity-Ligation-Assay-H00000369-M05-img0007.jpg)
![Western Blot: A-RAF Antibody [NBP2-55054] - Analysis using Anti-ARAF antibody NBP2-55054 (A) shows similar pattern to independent antibody NBP2-49479 (B).](http://images.novusbio.com/fullsize/A-RAF-Antibody-Western-Blot-NBP2-55054-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: A-RAF Antibody [NBP2-55054] - Analysis using Anti-ARAF antibody NBP2-55054 (A) shows similar pattern to independent antibody NBP2-49479 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: A-RAF Antibody [NBP2-55054] - Staining of human cell line HEK 293 shows localization to nucleus & nucleoli fibrillar center.](http://images.novusbio.com/fullsize/A-RAF-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55054-img0001.jpg)
![Western Blot: A-RAF Antibody [NBP2-49479] - Analysis using Anti-ARAF antibody NBP2-49479 (A) shows similar pattern to independent antibody NBP2-55054 (B).](http://images.novusbio.com/fullsize/A-RAF-Antibody-Western-Blot-NBP2-49479-img0006.jpg "<b>Independent Antibodies Validation. </b>Western Blot: A-RAF Antibody [NBP2-49479] - Analysis using Anti-ARAF antibody NBP2-49479 (A) shows similar pattern to independent antibody NBP2-55054 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: A-RAF Antibody [NBP2-49479] - Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/A-RAF-Antibody-Immunohistochemistry-Paraffin-NBP2-49479-img0005.jpg)
![Western Blot: A-RAF Antibody (4U9L7) [NBP3-16677] - Western blot analysis of extracts of various cell lines, using A-RAF antibody (NBP3-16677) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/A-RAF-Antibody-4U9L7-Western-Blot-NBP3-16677-img0002.jpg "Western Blot: A-RAF Antibody (4U9L7) [NBP3-16677] - Western blot analysis of extracts of various cell lines, using A-RAF antibody (NBP3-16677) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: A-RAF Antibody (4U9L7) [NBP3-16677] - Immunohistochemistry of paraffin-embedded human liver using A-RAF Rabbit mAb (NBP3-16677) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/A-RAF-Antibody-4U9L7-Immunohistochemistry-Paraffin-NBP3-16677-img0001.jpg)
![SDS-Page: A-RAF Recombinant Protein [H00000369-P01]](http://images.novusbio.com/fullsize/A-RAF-Recombinant-Protein-SDS-Page-H00000369-P01-img0001.jpg "SDS-Page: A-RAF Recombinant Protein [H00000369-P01]")
![Functional: Recombinant Human A-RAF Protein [H00000369-P01]](http://images.novusbio.com/fullsize/Recombinant-Human-A-RAF-Protein-Functional-H00000369-P01-img0003.jpg)
![Western Blot: A-RAF Antibody (M289) [NBP3-23355] - Western blot of human Jurkat cell lysate. The blot was probed with mouse monoclonal anti-A-Raf (N-terminal region) antibody at 1:500 (lane 1) and 1:2000 (lane 2).](http://images.novusbio.com/fullsize/nbp3-23355_mouse-a-raf-mab-m289-19920231856963.jpg "Western Blot: A-RAF Antibody (M289) [NBP3-23355] - Western blot of human Jurkat cell lysate. The blot was probed with mouse monoclonal anti-A-Raf (N-terminal region) antibody at 1:500 (lane 1) and 1:2000 (lane 2).")
![SDS-Page: Recombinant Human A-RAF Protein [H00000369-Q02] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-A-RAF-Protein-SDS-Page-H00000369-Q02-img0001.jpg "SDS-Page: Recombinant Human A-RAF Protein [H00000369-Q02] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![SDS-Page: Recombinant Human A-RAF Protein [H00000369-P02] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-A-RAF-Protein-SDS-Page-H00000369-P02-img0001.jpg "SDS-Page: Recombinant Human A-RAF Protein [H00000369-P02] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![SDS-Page: Recombinant Human A-RAF Protein [H00000369-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-A-RAF-Protein-SDS-Page-H00000369-Q01-img0001.jpg "SDS-Page: Recombinant Human A-RAF Protein [H00000369-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")