Hu,
Mu,
Pm,
Rt,
Pm,
Ma-Op,
RMSpecies Glossary
Reactivity: Hu, Mu, Pm, Rt, Pm, Ma-Op, RM
WB, Simple Western, IHC
Applications: WB, Simple Western, IHC
Host: Rabbit Polyclonal
Alt Catalog# IMG-5893A
Sample Size Available
Sizes: Sample Size Available
![Western Blot: CD97 Antibody [NBP2-24552] - Analysis of human Leukocyte Antigen CD97 in K562 cell lysate in the 1) absence and 2) presence of immunizing peptide, and 3) NIH 3T3 cell lysate using NBP2-24552 at 4 ug/ml.](http://images.novusbio.com/fullsize/CD97-Antibody-Western-Blot-NBP2-24552-img0001.jpg "Western Blot: CD97 Antibody [NBP2-24552] - Analysis of human Leukocyte Antigen CD97 in K562 cell lysate in the 1) absence and 2) presence of immunizing peptide, and 3) NIH 3T3 cell lysate using NBP2-24552 at 4 ug/ml.")
![Immunohistochemistry-Paraffin: CD97 Antibody [NBP2-24552] - IHC analysis of human spleen using NBP2-24552 at 10 ug/ml.](http://images.novusbio.com/fullsize/CD97-Antibody-Immunohistochemistry-Paraffin-NBP2-24552-img0002.jpg)
![Simple Western: CD97 Antibody [NBP2-24552] - Simple Western lane view shows a specific band for CD97 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/CD97-Antibody-Simple-Western-NBP2-24552-img0003.jpg)
![Immunohistochemistry-Paraffin: CD97 Antibody [NBP1-86935] - Staining of human spleen shows high expression.](http://images.novusbio.com/fullsize/CD97-Antibody-Immunohistochemistry-Paraffin-NBP1-86935-img0005.jpg "Immunohistochemistry-Paraffin: CD97 Antibody [NBP1-86935] - Staining of human spleen shows high expression.")
![Immunohistochemistry-Paraffin: CD97 Antibody [NBP1-86935] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize/CD97-Antibody-Immunohistochemistry-Paraffin-NBP1-86935-img0004.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD97 Antibody [NBP1-86935] - Staining in human spleen and cerebral cortex tissues using anti-ADGRE5 antibody. Corresponding ADGRE5 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CD97-Antibody-Immunohistochemistry-Paraffin-NBP1-86935-img0006.jpg)
![Western Blot: CD97 Antibody (SR2289) [NBP3-21846] - Western blot analysis using NBP3-21846 in U937 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21846_rabbit-cd97-mab-sr2289-672023106275.jpg "Western Blot: CD97 Antibody (SR2289) [NBP3-21846] - Western blot analysis using NBP3-21846 in U937 cell lysate.")
![Western Blot: CD97 Antibody [NBP2-99765] - Anti-CD97 rabbit polyclonal antibody at 1:500 dilution. Lane A: HL-60 Membrane Lysate. Lane B: K562 Membrane Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 92 kDa. Observed band size: 70 kDa](http://images.novusbio.com/fullsize/CD97-Antibody-Western-Blot-NBP2-99765-img0005.jpg "Western Blot: CD97 Antibody [NBP2-99765] - Anti-CD97 rabbit polyclonal antibody at 1:500 dilution. Lane A: HL-60 Membrane Lysate. Lane B: K562 Membrane Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 92 kDa. Observed band size: 70 kDa")
![Immunocytochemistry/Immunofluorescence: CD97 Antibody [NBP2-99765] - Immunofluorescence staining of CD97 in HeLa cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-human CD97 polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cell membrane.](http://images.novusbio.com/fullsize/CD97-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-99765-img0002.jpg)
![Immunoprecipitation: CD97 Antibody [NBP2-99765] - CD97 was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate 2 ul anti-CD97 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G. Primary antibody: Anti-CD97 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 92 kDa. Observed band size: 92 kDa](http://images.novusbio.com/fullsize/CD97-Antibody-Immunoprecipitation-NBP2-99765-img0004.jpg)
![Immunohistochemistry: CD97 Antibody (3A7) [NBP3-26673] - Image of CD97 Antibody (3A7) diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26673_rabbit-cd97-mab-3a7-262202414471599.jpg "Immunohistochemistry: CD97 Antibody (3A7) [NBP3-26673] - Image of CD97 Antibody (3A7) diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![Immunohistochemistry: CD97 Antibody (3A7) [NBP3-26673] - Image of CD97 Antibody (3A7) diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26673_rabbit-cd97-mab-3a7-262202415475633.jpg)
![Immunocytochemistry/Immunofluorescence: CD97 Antibody (3A7) [NBP3-26673] - Staining of Hela cells with CD97 Antibody (3A7) at 1:87.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26673_rabbit-cd97-mab-3a7-282202412475124.jpg)
![Western Blot: CD97 Antibody (SR1972) [NBP3-22085] - Western blot analysis using NBP3-22085 in human tonsil cell lysate.](http://images.novusbio.com/fullsize/nbp3-22085_rabbit-cd97-mab-sr1972-572023159552.jpg "Western Blot: CD97 Antibody (SR1972) [NBP3-22085] - Western blot analysis using NBP3-22085 in human tonsil cell lysate.")
![Flow Cytometry: CD97 Antibody (MEM-180) [NB500-425] - Using the FITC direct conjugate Surface staining of human peripheral blood with anti-CD97 (MEM-180) FITC.](http://images.novusbio.com/fullsize/CD97-Antibody-MEM-180-Flow-Cytometry-NB500-425-img0002.jpg "Flow Cytometry: CD97 Antibody (MEM-180) [NB500-425] - Using the FITC direct conjugate Surface staining of human peripheral blood with anti-CD97 (MEM-180) FITC.")
![Flow Cytometry: CD97 Antibody (MEM-180) [NB500-425] - Surface staining of human peripheral blood with anti-CD97 (MEM-180) purified, GAM-APC.](http://images.novusbio.com/fullsize/CD97-Antibody-MEM-180-Flow-Cytometry-NB500-425-img0001.jpg)
![Western Blot: CD97 Antibody [NBP2-99507] - Anti-CD97 rabbit polyclonal antibody at 1:500 dilution. Lane A: NIH/3T3 Whole Cell Lysate. Lane B: HepG2 Whole Cell Lysate. Lane C: Raw264.7 Whole Cell Lysate. Lane D: Mouse spleen tissue lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 92 kDa](http://images.novusbio.com/fullsize/CD97-Antibody-Western-Blot-NBP2-99507-img0002.jpg "Western Blot: CD97 Antibody [NBP2-99507] - Anti-CD97 rabbit polyclonal antibody at 1:500 dilution. Lane A: NIH/3T3 Whole Cell Lysate. Lane B: HepG2 Whole Cell Lysate. Lane C: Raw264.7 Whole Cell Lysate. Lane D: Mouse spleen tissue lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 92 kDa")
![Immunoprecipitation: CD97 Antibody [NBP2-99507] - CD97 was immunoprecipitated using: Lane A: 0.5 mg NIH/3T3 Whole Cell Lysate. 4 ul anti-CD97 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-CD97 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 92 kDa. Observed band size: 70 kDa](http://images.novusbio.com/fullsize/CD97-Antibody-Immunoprecipitation-NBP2-99507-img0003.jpg)
![Western Blot: CD97 Antibody [NBP2-99764] - Anti-CD97 rabbit polyclonal antibody at 1:500 dilution. Lane A: HL-60 Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 92 kDa. Observed band size: 92 kDa](http://images.novusbio.com/fullsize/CD97-Antibody-Western-Blot-NBP2-99764-img0004.jpg "Western Blot: CD97 Antibody [NBP2-99764] - Anti-CD97 rabbit polyclonal antibody at 1:500 dilution. Lane A: HL-60 Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 92 kDa. Observed band size: 92 kDa")
![Immunoprecipitation: CD97 Antibody [NBP2-99764] - CD97 was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. Lane B: 0.5 mg SW480 Whole Cell Lysate0.5 ul anti-CD97 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-CD97 rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 92 kDa. Observed band size: 92 kDa](http://images.novusbio.com/fullsize/CD97-Antibody-Immunoprecipitation-NBP2-99764-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: CD97 Antibody (024) [NBP2-89876] - Staining of Human CD97 in JURKAT cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human CD97 monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cells membranes.](http://images.novusbio.com/fullsize/CD97-Antibody-024-Immunocytochemistry-Immunofluorescence-NBP2-89876-img0001.jpg "Immunocytochemistry/Immunofluorescence: CD97 Antibody (024) [NBP2-89876] - Staining of Human CD97 in JURKAT cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human CD97 monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cells membranes.")
![Flow Cytometry: CD97 Antibody (024) [NBP2-89876] - Analysis of Human CD97 expression on K562 cells. Cells were stained with purified anti-Human CD97, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/CD97-Antibody-024-Flow-Cytometry-NBP2-89876-img0002.jpg)
![SDS-Page: Recombinant Human CD97 Protein [H00000976-P02] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00000976-P02-1.jpg "SDS-Page: Recombinant Human CD97 Protein [H00000976-P02] - 12.5% SDS-PAGE Stained with Coomassie Blue.")