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![Western Blot: Peripheral Node Addressin Antibody (MECA-79R) [NBP2-78792] - Total protein from human Tonsil, Lymph node, Spleen and mouse Spleen was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-PNAd in blocking buffer and detected with an anti-rat HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/Peripheral-Node-Addressin-Antibody-MECA-79R-Western-Blot-NBP2-78792-img0002.jpg "Western Blot: Peripheral Node Addressin Antibody (MECA-79R) [NBP2-78792] - Total protein from human Tonsil, Lymph node, Spleen and mouse Spleen was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-PNAd in blocking buffer and detected with an anti-rat HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.")
![Immunohistochemistry-Paraffin: Peripheral Node Addressin Antibody (MECA-79R) [NBP2-78792] - Analysis of FFPE mouse adipose tissue section (with lymph node areas) using Peripheral Node Addressin antibody (clone MECA-79) at 1:100. The staining was developed with HRP-DAB detection method and the counterstaining was performed using hematoxylin. This Peripheral Node Addressin antibody generated a strong and specific staining of MECA-79 antigen in the the cytoplasm and the membranes of high endothelial venules (HEVs) aka peripheral lymph node addressin (PNAd) in lymph node areas of tested adipose tissue section.](http://images.novusbio.com/fullsize/Peripheral-Node-Addressin-Antibody-MECA-79R-Immunohistochemistry-Paraffin-NBP2-78792-img0003.jpg)
![Immunohistochemistry-Paraffin: Peripheral Node Addressin Antibody (MECA-79R) [NBP2-78792] - Analysis of FFPE human tonsil tissue section using Peripheral Node Addressin antibody (clone MECA-79R) at 1:100. The staining was developed with HRP-DAB detection method and the counterstaining was performed using hematoxylin.](http://images.novusbio.com/fullsize/Peripheral-Node-Addressin-Antibody-MECA-79R-Immunohistochemistry-Paraffin-NBP2-78792-img0001.jpg)
![Immunohistochemistry: Peripheral Node Addressin Antibody (MECA-79R) [NBP2-78792] - Local release of anti-IL-6 from IMB suppresses LN fibrosis. The skin allografts were harvested at 7 days post-transplantation. Lymphatic vessel expansion (Lyve-1+) and HEV elongation (MECA79+) were similar between all groups. Dense staining of collagen I and PDPN was seen in DLNs harvested from control and GelMA group compared to those from GelMA/anti-IL-6 group. (representative images from 4 different mice per group). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-42349-w), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Peripheral-Node-Addressin-Antibody-MECA-79R-Immunohistochemistry-NBP2-78792-img0004.jpg)
![Flow Cytometry: Rat IgG2a Isotype Control (KLH/G2a-1-1) [NBP1-51104] - A cell surface stain was performed on RAW246.7 cells with B7-H1/PD-L1/CD274 (MIH5) antibody NBP1-43262APC (blue) and a matched isotype control NBP1-51104APC (orange). Cells were incubated in an antibody dilution of 0.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.](http://images.novusbio.com/fullsize/Rat-IgG2a-Isotype-Control-KLH-G2a-1-1-Flow-Cytometry-NBP1-51104-img0001.jpg "Flow Cytometry: Rat IgG2a Isotype Control (KLH/G2a-1-1) [NBP1-51104] - A cell surface stain was performed on RAW246.7 cells with B7-H1/PD-L1/CD274 (MIH5) antibody NBP1-43262APC (blue) and a matched isotype control NBP1-51104APC (orange). Cells were incubated in an antibody dilution of 0.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.")
![Immunofluorescence: Rat IgG2a Isotype Control (KLH/G2a-1-1) [NBP1-51104] - Frozen BALB/c mouse intestine tissue was stained with Rat IgG2a-UNLB isotype control (SB Cat. No. 0117-01; left) and Rat Anti-Mouse CD104-UNLB (SB Cat. No. 1855-01; right) followed by Goat Anti-Rat IgG(H+L), Mouse ads-BIOT (SB Cat. No. 3050-08), Streptavidin-FITC (SB Cat. No. 7100-02), and DAPI.](http://images.novusbio.com/fullsize/Rat-IgG2a-Isotype-Control-KLH-G2a-1-1-Immunofluorescence-NBP1-51104-img0002.jpg)
![Flow Cytometry: CD8 beta Antibody (341) - Low Endotoxin and Azide Free [NBP3-05298] - Separation of murine CD8b positive cells (red-filled) from CD8b negative cells (black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension stained using anti-rat CD8b (341) purified antibody (concentration in sample 1 ug/ml, GAM APC).](http://images.novusbio.com/fullsize/CD8-beta-Antibody-341-Low-Endotoxin-and-Azide-Free-Flow-Cytometry-NBP3-05298-img0002.jpg "Flow Cytometry: CD8 beta Antibody (341) - Low Endotoxin and Azide Free [NBP3-05298] - Separation of murine CD8b positive cells (red-filled) from CD8b negative cells (black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension stained using anti-rat CD8b (341) purified antibody (concentration in sample 1 ug/ml, GAM APC).")
![Flow Cytometry: CD8 beta Antibody (341) - Low Endotoxin and Azide Free [NBP3-05298] - Surface staining pattern of rat splenocyte suspension stained using anti-rat CD8b (341) purified antibody (concentration in sample 1 ug/ml, GAM APC).](http://images.novusbio.com/fullsize/CD8-beta-Antibody-341-Low-Endotoxin-and-Azide-Free-Flow-Cytometry-NBP3-05298-img0001.jpg)
![ELISA: Rat IgG1 Kappa Light Chain Isotype Control (KLH/G1-2-2) [NBP1-51817] - FLISA plate was coated with Mouse Anti-Rat IgG1-UNLB (SB Cat. No. 3061-01), Mouse Anti-Rat IgG2a-UNLB (SB Cat. No. 3065-01), Mouse Anti-Rat IgG2b-UNLB (SB Cat. No. 3070-01), Mouse Anti-Rat IgG2c-UNLB (SB Cat. No. 3075-01), and Mouse Anti-Rat IgM-UNLB (SB Cat. No. 3080-01). Serially diluted Rat IgG1-PE (SB Cat. No. 0116-09) was captured and fluorescence intensity quantified.](http://images.novusbio.com/fullsize/Rat-IgG1-Kappa-Light-Chain-Isotype-Control-KLH-G1-2-2-ELISA-NBP1-51817-img0001.jpg "ELISA: Rat IgG1 Kappa Light Chain Isotype Control (KLH/G1-2-2) [NBP1-51817] - FLISA plate was coated with Mouse Anti-Rat IgG1-UNLB (SB Cat. No. 3061-01), Mouse Anti-Rat IgG2a-UNLB (SB Cat. No. 3065-01), Mouse Anti-Rat IgG2b-UNLB (SB Cat. No. 3070-01), Mouse Anti-Rat IgG2c-UNLB (SB Cat. No. 3075-01), and Mouse Anti-Rat IgM-UNLB (SB Cat. No. 3080-01). Serially diluted Rat IgG1-PE (SB Cat. No. 0116-09) was captured and fluorescence intensity quantified.")