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NB100-306

ATM [p Ser1981] Antibody (10H11.E12)

(1 Review)

(29 Publications)

0.1 ml

NB100-306

Immunohistochemistry: ATM [p Ser1981] Antibody (10H11.E12) [NB100-306] - ATM [p Ser1981] antibody (10H11.E12) [NB100-306] was tested in mouse spleen using DAB with hematoxylin counterstain.

Western Blot: ATM [p Ser1981] Antibody (10H11.E12) [NB100-306] - Analysis of ATM-kinase, using ATM [p Ser1981] antibody (10H11.E12) [NB100-306]. Sample: Irradiated or peroxidated human fibroblasts. Observed molecular weight 370 kDa. Theoretical molecular weight 351 kDa.

Immunocytochemistry/Immunofluorescence: ATM [p Ser1981] Antibody (10H11.E12) [NB100-306] - Human lymphoblastoid cells stained with ATM [p Ser1981] Antibody (10H11.E12). ICC/IF image submitted by a verified customer review.

<b>Biological Strategies Validation. </b>Western Blot: ATM [p Ser1981] Antibody (10H11.E12) [NB100-306] - MCELNs decreased the DNA damage of H9C2 cells after radiation. Western blot images of p-ATM (NB100-306) and ATM (NB100-309) in H9C2 cells after 48 h of culture with indicated treatment. IR (-/+): 0/16 Gy X-ray; MCELNs (-/+): 0/10 ug/mL. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35509278/) licensed under a CC-BY license.

A. TRF2 or DSP1 pull down by telomere targeting CASID with the condition of MST-312 treatment (left) or hTERT knockdown (right) in MDA-MB-231cells. B. Southern blot of telomere DNA from MDA-MB-231cells treated with MST-312 or with hTERT knockdown. C. Top, Dot blot of telomere DNA repeats from the chromatin pull down by DSP from the cells treated by MST-312 (left) or hTERT knockdown (right). Alu DNA repeats serve as negative control. Bottom, the histogram shows the quantitated results of the dot blots. The triple asterisks (***) indicates a p-value less than 0.01. D. Fluorescence image of DSP-EGFP transfected MDA-MB-231 cells with the conditions of MST-312 treatment or hTERT knockdown. E. Pull down assay of DSP by CASID targeting telomere in WI38 cells with or without ectopically expression of hTERT gene. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31595153), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Ca(-)

Species Glossary

Reactivity: Hu, Mu, Rt, Ca(-)

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-306AF350
Alexa Fluor 405	NB100-306AF405
Alexa Fluor 488	NB100-306AF488
Alexa Fluor 532	NB100-306AF532
Alexa Fluor 594	NB100-306AF594
Alexa Fluor 647	NB100-306AF647
Alexa Fluor 700	NB100-306AF700
Alexa Fluor 750	NB100-306AF750
Biotin	NB100-306B
DyLight 350	NB100-306UV
DyLight 405	NB100-306V
DyLight 488	NB100-306G
DyLight 550	NB100-306R
DyLight 594	NB100-306DL594
DyLight 650	NB100-306C
DyLight 680	NB100-306FR
DyLight 755	NB100-306IR
FITC	NB100-306F
HRP	NB100-306H
Janelia Fluor 549	NB100-306JF549
Janelia Fluor 646	NB100-306JF646
mFluor Violet 450 SE	NB100-306MFV450
mFluor Violet 500 SE	NB100-306MFV500
mFluor Violet 610 SE	NB100-306MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-80586

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AF1655

ATM [p Ser1981] Antibody

(1 Review)

(29 Publications)

100 μg

AF1655

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 1 µM camptothecin (CPT) for 1 hour. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human/Mouse/Rat Phospho-ATM (S1981) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1655) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-ATM (S1981) was detected at approximately 370 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda-phosphatase (lambda-PPase) for 1 hour. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 1 µM Camptothecin (CPT) for 1 hour, loaded at 0.2 mg/mL. A specific band was detected for Phospho-ATM (S1981) at approximately 270 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-ATM (S1981) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1655). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.

Resveratrol activates ATM in transformed human cell lines.(a, b) Human HEK293T cells were treated with 0.01 mM ATM inhibitor (KU-55933) or vehicle (DMSO) for 1 hour, followed by incubation in media also containing 0.1 mM resveratrol (or DMSO), as indicated. After 30 minutes incubation with resveratrol, H2O2 (0.1 mM) or bleomycin (1 µg/ml) was added as indicated for 30 minutes before harvesting. Western blotting was performed with with antibodies directed against ATM, phospho-ATM Ser-1981, p53, and phospho-p53 Ser-15 as indicated. (c, d) Human HEK293T cells were treated with resveratrol, bleomycin, or both as in (a) and probed for gamma -H2AX foci by immunofluorescence. Cell images (291, 267, 216, and 260 cells, respectively) were analyzed for foci using Image J software, and the average number of foci per cell as well as the percentage of cells containing >5 foci were quantitated. (e, f) Human HCT116 cells were incubated with KU-55933, resveratrol, H2O2, and bleomycin as in (a, b) but additional phosphorylation targets were examined using antibodies directed against Smc1, phospho-Smc1 Ser-957, Kap1, phospho-Kap1 Ser-824, Nbs1, phospho-Nbs1 Ser-343, Chk2, and phospho-Chk2 Thr-68 as indicated. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0097969), licensed under a CC-BY license. Not internally tested by R&D Systems.

Resveratrol activates ATM in transformed human cell lines.(a, b) Human HEK293T cells were treated with 0.01 mM ATM inhibitor (KU-55933) or vehicle (DMSO) for 1 hour, followed by incubation in media also containing 0.1 mM resveratrol (or DMSO), as indicated. After 30 minutes incubation with resveratrol, H2O2 (0.1 mM) or bleomycin (1 µg/ml) was added as indicated for 30 minutes before harvesting. Western blotting was performed with with antibodies directed against ATM, phospho-ATM Ser-1981, p53, and phospho-p53 Ser-15 as indicated. (c, d) Human HEK293T cells were treated with resveratrol, bleomycin, or both as in (a) and probed for gamma -H2AX foci by immunofluorescence. Cell images (291, 267, 216, and 260 cells, respectively) were analyzed for foci using Image J software, and the average number of foci per cell as well as the percentage of cells containing >5 foci were quantitated. (e, f) Human HCT116 cells were incubated with KU-55933, resveratrol, H2O2, and bleomycin as in (a, b) but additional phosphorylation targets were examined using antibodies directed against Smc1, phospho-Smc1 Ser-957, Kap1, phospho-Kap1 Ser-824, Nbs1, phospho-Nbs1 Ser-343, Chk2, and phospho-Chk2 Thr-68 as indicated. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0097969), licensed under a CC-BY license. Not internally tested by R&D Systems.

Resveratrol activates ATM in transformed human cell lines.(a, b) Human HEK293T cells were treated with 0.01 mM ATM inhibitor (KU-55933) or vehicle (DMSO) for 1 hour, followed by incubation in media also containing 0.1 mM resveratrol (or DMSO), as indicated. After 30 minutes incubation with resveratrol, H2O2 (0.1 mM) or bleomycin (1 µg/ml) was added as indicated for 30 minutes before harvesting. Western blotting was performed with with antibodies directed against ATM, phospho-ATM Ser-1981, p53, and phospho-p53 Ser-15 as indicated. (c, d) Human HEK293T cells were treated with resveratrol, bleomycin, or both as in (a) and probed for gamma -H2AX foci by immunofluorescence. Cell images (291, 267, 216, and 260 cells, respectively) were analyzed for foci using Image J software, and the average number of foci per cell as well as the percentage of cells containing >5 foci were quantitated. (e, f) Human HCT116 cells were incubated with KU-55933, resveratrol, H2O2, and bleomycin as in (a, b) but additional phosphorylation targets were examined using antibodies directed against Smc1, phospho-Smc1 Ser-957, Kap1, phospho-Kap1 Ser-824, Nbs1, phospho-Nbs1 Ser-343, Chk2, and phospho-Chk2 Thr-68 as indicated. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0097969), licensed under a CC-BY license. Not internally tested by R&D Systems.

Resveratrol activates ATM in transformed human cell lines.(a, b) Human HEK293T cells were treated with 0.01 mM ATM inhibitor (KU-55933) or vehicle (DMSO) for 1 hour, followed by incubation in media also containing 0.1 mM resveratrol (or DMSO), as indicated. After 30 minutes incubation with resveratrol, H2O2 (0.1 mM) or bleomycin (1 µg/ml) was added as indicated for 30 minutes before harvesting. Western blotting was performed with with antibodies directed against ATM, phospho-ATM Ser-1981, p53, and phospho-p53 Ser-15 as indicated. (c, d) Human HEK293T cells were treated with resveratrol, bleomycin, or both as in (a) and probed for gamma -H2AX foci by immunofluorescence. Cell images (291, 267, 216, and 260 cells, respectively) were analyzed for foci using Image J software, and the average number of foci per cell as well as the percentage of cells containing >5 foci were quantitated. (e, f) Human HCT116 cells were incubated with KU-55933, resveratrol, H2O2, and bleomycin as in (a, b) but additional phosphorylation targets were examined using antibodies directed against Smc1, phospho-Smc1 Ser-957, Kap1, phospho-Kap1 Ser-824, Nbs1, phospho-Nbs1 Ser-343, Chk2, and phospho-Chk2 Thr-68 as indicated. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0097969), licensed under a CC-BY license. Not internally tested by R&D Systems.

DEK is necessary for the activation of gamma H2AX after ionizing radiation.(a) MEFs were treated with 10 Gy of gamma ionizing radiation (IR) and stained for gamma H2AX. Representative cross sections from z-stack confocal images are shown. (b) Quantification of cells with >10 gamma H2AX foci in (A). At least 50 cells per time point were counted in each biological replicate, and all foci within the z-stack were quantified. DEK−/− MEFs were unable to activate gamma H2AX above baseline after irradiation. (paired t-test, n = 3, mean ± SEM). (c) Quantification of the average total number of gamma H2AX foci in each cell from (A). (paired t-test, n = 3, mean ± SEM) The number of foci per cell remained unchanged in DEK−/− MEFs after irradiation. (d) Western blot analysis of gamma H2AX in MEFs at 6 hs following irradiation with 10 Gy. (e) Western blot analysis of C33A human cancer cells infected with adenovirus 48 hr prior to receiving 10 Gy IR (see also Supplementary Fig. S1). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28317934), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF1655-SP

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