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![Western Blot: eIF4E [p Ser209] Antibody [NB100-81898] - Analysis of extracts from SK-BR-3 cells, untreated or insulin and EGF treated, and pretreated with U0126 cells, using NB100-81898](http://images.novusbio.com/fullsize/eIF4E-[p-Ser209]-Antibody-Western-Blot-NB100-81898-img0009.jpg "Western Blot: eIF4E [p Ser209] Antibody [NB100-81898] - Analysis of extracts from SK-BR-3 cells, untreated or insulin and EGF treated, and pretreated with U0126 cells, using NB100-81898")
![Immunocytochemistry/Immunofluorescence: eIF4E [p Ser209] Antibody [NB100-81898] - Staining of methanol-fixed MCF cells using NB100-81898](http://images.novusbio.com/fullsize/eIF4E-[p-Ser209]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-81898-img0005.jpg)
![Immunohistochemistry-Paraffin: eIF4E [p Ser209] Antibody [NB100-81898] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using NB100-81898 (left) or the same antibody preincubated with blocking peptide (right).](http://images.novusbio.com/fullsize/eIF4E-[p-Ser209]-Antibody-Immunohistochemistry-NB100-81898-img0006.jpg)
![Western Blot: eIF4E Antibody [NB100-58833] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-eIF4E antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/eIF4E-Antibody-Western-Blot-NB100-58833-img0002.jpg "Western Blot: eIF4E Antibody [NB100-58833] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-eIF4E antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: eIF4E Antibody [NB100-58833] - Detection of human eIF4E by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-eIF4E antibody NB100-58833 used for IP at 3 ug per reaction. eIF4E was also immunoprecipitated by another rabbit anti-eIF4E antibody. For blotting immunoprecipitated eIF4E, NB100-58833 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/eIF4E-Antibody-Immunoprecipitation-NB100-58833-img0003.jpg)
![Western Blot: eIF4E Antibody [NBP1-57195] - C-terminal region validated by WB using Mouse Brain lysate.](http://images.novusbio.com/fullsize/eIF4E-Antibody-Western-Blot-NBP1-57195-img0005.jpg "Western Blot: eIF4E Antibody [NBP1-57195] - C-terminal region validated by WB using Mouse Brain lysate.")
![Immunohistochemistry-Paraffin: eIF4E Antibody [NBP1-57195] - Human Lung respiratory epithelium tissue, 5ug/ml.](http://images.novusbio.com/fullsize/eIF4E-Antibody-Immunohistochemistry-Paraffin-NBP1-57195-img0004.jpg)
![Western Blot: eIF4E Antibody [NBP1-57195]](http://images.novusbio.com/fullsize/eIF4E-Antibody-Western-Blot-NBP1-57195-img0002.jpg)
![Western Blot: eIF4E Antibody [NBP1-57195] - HEK293 lysate, mouse brain extract, Antibody Titration: 0.2-1 ug/ml](http://images.novusbio.com/fullsize/eIF4E-Antibody-Western-Blot-NBP1-57195-img0003.jpg)
![Western Blot: eIF4E Antibody [NBP2-57098] - Analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize/eIF4E-Antibody-Western-Blot-NBP2-57098-img0002.jpg "Western Blot: eIF4E Antibody [NBP2-57098] - Analysis in human cell line RT-4 and human cell line U-251 MG.")
![Immunocytochemistry/Immunofluorescence: eIF4E Antibody [NBP2-57098] - Staining of human cell line U-2 OS shows localization to cytosol & cytoplasmic bodies. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/eIF4E-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57098-img0001.jpg)
![Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 | Lane 2: RT4 | Lane 3: U-251 MG | Lane 4: Human Plasma | Lane 5: Liver | Lane 6: Tonsil](http://images.novusbio.com/fullsize/nbp3-21317_rabbit-eif4e-pab-4520231553437.jpg)
![Western Blot: eIF4E Antibody [NBP2-98868] - Anti-eIF4E rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lane B: K562 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 25 kDa. Observed band size: 25 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/eIF4E-Antibody-Western-Blot-NBP2-98868-img0001.jpg "Western Blot: eIF4E Antibody [NBP2-98868] - Anti-eIF4E rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lane B: K562 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 25 kDa. Observed band size: 25 kDa (We are unsure as to the identity of these extra bands).")
![Immunohistochemistry-Paraffin: eIF4E Antibody [NBP2-98867] - Immunochemical staining of human eIF4E in human hepatoma with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/eIF4E-Antibody-Immunohistochemistry-Paraffin-NBP2-98867-img0002.jpg "Immunohistochemistry-Paraffin: eIF4E Antibody [NBP2-98867] - Immunochemical staining of human eIF4E in human hepatoma with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: eIF4E Antibody [NBP2-98867] - Immunochemical staining of human eIF4E in human breast carcinoma with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/eIF4E-Antibody-Immunohistochemistry-Paraffin-NBP2-98867-img0001.jpg)