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4 Results for "novus"

MAB2616

TLR2 Antibody (383936)

(25 Publications)

100 μg

MAB2616

The synthetic tripalmitoylated lipopeptide Pam3CSK4 stimulates IL‑8 secretion in the HEK293 human embryonic kidney cell line transfected with human TLR2, in a dose-dependent manner (orange line), as measured by the Human CXCL8/IL‑8 Quantikine ELISA Kit (Catalog # D8000C). IL‑8 secretion elicited by Pam3CSK4 (0.5 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human TLR2 Monoclonal Antibody (Catalog # MAB2616). The ND50 is typically 0.03-0.15 µg/mL.

Human whole blood monocytes were stained with Mouse Anti-Human TLR2 Monoclonal Antibody (Catalog # MAB2616, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B).

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB2616U-100UG
Alexa Fluor 405	FAB2616V-100UG
Alexa Fluor 488	FAB2616G-100UG
Alexa Fluor 532	FAB2616X
Alexa Fluor 594	FAB2616T-100UG
Alexa Fluor 647	FAB2616R-100UG
Alexa Fluor 700	FAB2616N
Alexa Fluor 750	FAB2616S-100UG
Allophycocyanin	FAB2616A
Allophycocyanin/Cy7	FAB2616APCCY7
Biotin	FAB2616B
DyLight 350	FAB2616D
DyLight 405	FAB2616E
DyLight 488	FAB2616K
DyLight 550	FAB2616L
DyLight 594	FAB2616M
DyLight 650	FAB2616W
DyLight 680	FAB2616Y
DyLight 755	FAB2616Z
HRP	FAB2616H
Janelia Fluor 549	FAB2616I
Janelia Fluor 646	FAB2616J
PE/Atto594	FAB2616PEATT594
PE/Cy5.5	FAB2616PECY55
PE/Cy7	FAB2616PECY7
PerCP (Peridinin-chlorophyll Protein Complex)	FAB2616C
Phycoerythrin	FAB2616P
mFluor Violet 450 SE	FAB2616MFV450
mFluor Violet 500 SE	FAB2616MFV500
mFluor Violet 610 SE	FAB2616MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB2616C
Carrier Free	FAB2616P
Carrier Free	FAB2616A
Carrier Free	FAB2616N
Carrier Free	MAB2616-SP
Carrier Free	FAB2616R-100UG
Carrier Free	FAB2616G-100UG
Carrier Free	FAB2616V-100UG
Carrier Free	FAB2616U-100UG
Carrier Free	FAB2616T-100UG
Carrier Free	FAB2616S-100UG

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AF2616

TLR2 Antibody [Unconjugated]

(17 Publications)

100 μg

AF2616

Western blot shows lysate of human lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TLR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2616) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TLR2 at approximately 90-100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF2616

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF2616-SP

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MAB1530

TLR2 Antibody (203325) [Unconjugated]

(2 Reviews)

(6 Publications)

500 μg

MAB1530

Raw264.7 cells stimulated with 100 ng/mL LPS overnight were stained with Rat Anti-Mouse TLR2 Monoclonal Antibody (Catalog # MAB1530, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Fluorescein-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0104B). View our protocol for Staining Membrane-associated Proteins.

TLR2Ab suppressed the expression of endogenous TLR2 agonists and interaction of TLR2 with HMGB1.(A) Expression of HMGB1 and Hsp70 were reduced in TLR2Ab-treated mice, but not in TLR4Ab group. (B) TLR2Ab and TLR4Ab suppressed its downstream signaling pathway. Data are shown as mean ± SEM (n = 5/group). (C) TLR2Ab inhibited the interaction of TLR2 with HMGB1 as indicated by co-immunoprecipitation and western blot. Data are representatives of two experiments with identical results. (D) TLR2Ab decreased the co-localization of TLR2 and HMGB1 as shown by confocal microscopy. Data are representatives of two experiments with identical results. #p<0.05, ##p<0.01, ###p<0.001 versus saline-treated group; *p<0.05, **p<0.01, ***p<0.001 versus Dox+IgG-treated group. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22808256), licensed under a CC-BY license. Not internally tested by R&D Systems.

TLR2Ab suppressed the expression of endogenous TLR2 agonists and interaction of TLR2 with HMGB1.(A) Expression of HMGB1 and Hsp70 were reduced in TLR2Ab-treated mice, but not in TLR4Ab group. (B) TLR2Ab and TLR4Ab suppressed its downstream signaling pathway. Data are shown as mean ± SEM (n = 5/group). (C) TLR2Ab inhibited the interaction of TLR2 with HMGB1 as indicated by co-immunoprecipitation and western blot. Data are representatives of two experiments with identical results. (D) TLR2Ab decreased the co-localization of TLR2 and HMGB1 as shown by confocal microscopy. Data are representatives of two experiments with identical results. #p<0.05, ##p<0.01, ###p<0.001 versus saline-treated group; *p<0.05, **p<0.01, ***p<0.001 versus Dox+IgG-treated group. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22808256), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

,

Applications: ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB1530U-100UG
Alexa Fluor 405	FAB1530V-100UG
Alexa Fluor 488	FAB1530G-100UG
Alexa Fluor 532	FAB1530X
Alexa Fluor 594	FAB1530T-100UG
Alexa Fluor 647	FAB1530R-100UG
Alexa Fluor 700	FAB1530N-100UG
Alexa Fluor 750	FAB1530S-100UG
Allophycocyanin/Cy7	FAB1530APCCY7
Biotin	FAB1530B
DyLight 350	FAB1530D
DyLight 405	FAB1530E
DyLight 488	FAB1530K
DyLight 550	FAB1530L
DyLight 594	FAB1530M
DyLight 650	FAB1530W
DyLight 680	FAB1530Y
DyLight 755	FAB1530Z
Fluorescein	FAB1530F
HRP	FAB1530H
Janelia Fluor 549	FAB1530I
Janelia Fluor 646	FAB1530J
PE	FAB1530P
PE/Atto594	FAB1530PEATT594
PE/Cy5.5	FAB1530PECY55
PE/Cy7	FAB1530PECY7
PerCP	FAB1530C

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB1530F
Carrier Free	MAB1530-SP
Carrier Free	FAB1530V-100UG
Carrier Free	FAB1530U-100UG
Carrier Free	FAB1530T-100UG
Carrier Free	FAB1530S-100UG
Carrier Free	FAB1530R-100UG
Carrier Free	FAB1530N-100UG
Carrier Free	FAB1530G-100UG

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NBP2-27165

TLR2 Antibody (11G5) - BSA Free

(2 Publications)

0.1 mg

NBP2-27165

Western Blot: TLR2 Antibody (11G5) [NBP2-27165] - Analysis of TLR2 antibody. Mouse RAW cell lysate was probed using TLR2 antibody at 5 ug/ml. goat anti-rat Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Flow Cytometry: TLR2 Antibody (11G5) [NBP2-27165] - Cell surface analysis using TLR2 antibody. Thyoglycollate-elicited peritoneal macrophages from C57BL/6 mice were tested with 2.5 ug of isotype control antibody (green) and 2.5 ug of TLR2 antibody (red).

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, ,

Applications: , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Alt Catalog#

IMG-6720A

Alt Catalog# IMG-6720A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-27166AF350
Alexa Fluor 405	NBP2-27166AF405
Alexa Fluor 405	NBP2-27165AF405
Alexa Fluor 488	NBP2-27166AF488
Alexa Fluor 488	NBP2-27165AF488
Alexa Fluor 532	NBP2-27166AF532
Alexa Fluor 594	NBP2-27166AF594
Alexa Fluor 647	NBP2-27166AF647
Alexa Fluor 700	NBP2-27166AF700
Alexa Fluor 750	NBP2-27166AF750
Allophycocyanin	NBP2-27166APC
Biotin	NBP2-27166B
DyLight 350	NBP2-27166UV
DyLight 405	NBP2-27166V
DyLight 488	NBP2-27166G
DyLight 550	NBP2-27166R
DyLight 594	NBP2-27165DL594
DyLight 650	NBP2-27166C
DyLight 680	NBP2-27166FR
DyLight 755	NBP2-27166IR
FITC	NBP2-27166F
HRP	NBP2-27166H
Janelia Fluor 549	NBP2-27166JF549
Janelia Fluor 646	NBP2-27166JF646
PE	NBP2-27166PE
PerCP	NBP2-27166PCP

Available Formulations

Formulation	Catalog #	Availability	Price
Azide Free	NBP2-27166
BSA Free	NBP2-27165SS

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