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4 Results for "novus"

MAB85501

SerpinB2 Antibody (930119) [Unconjugated]

(1 Publication)

100 μg

MAB85501

Western blot shows lysates of K562 human chronic myelogenous leukemia parental cell line and Serpin B2 knockout K562 cell line (KO) untreated (-) or treated (+) with 300nM PMA for overnight. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Serpin B2 Monoclonal Antibody (Catalog # MAB85501) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Serpin B2 at approximately 40 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. HSP60 (Catalog # MAB1800) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

<b>Genetic Strategies Validation. </b>Western blot shows lysates of HEK001 human epidermal keratinocyte cell line (untreated) and U937 human histiocytic lymphoma cell line untreated (-) or treated (+) with PMA. PVDF membrane was probed with 0.05 µg/mL of Mouse Anti-Human Serpin B2 Monoclonal Antibody (Catalog # MAB85501) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Serpin B2 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of U937 human histiocytic lymphoma cell line untreated (-) or treated (+) with PMA, loaded at 0.2 mg/mL. Specific bands were detected for Serpin B2 at approximately 45 & 50 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Serpin B2 Monoclonal Antibody (Catalog # MAB85501) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB85501-SP

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NBP1-83163

SerpinB2 Antibody

(4 Publications)

0.1 ml

NBP1-83163

Immunohistochemistry-Paraffin: SerpinB2 Antibody [NBP1-83163] - Staining of human esophagus shows cytoplasmic positivity in squamous epithelial cells.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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9206-PI

Recombinant Human Serpin B2/PAI-2 Protein, CF

(1 Publication)

25 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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DY8550-05

Human Serpin B2 DuoSet ELISA, 5 Plate

(1 Publication)

1 Kit (for 5 Plates)

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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