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![ELISA: SARS-CoV-2 Spike RBD Antibody (HL1866) - Omicron Variant - Azide and BSA Free [NBP3-25671] - Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BQ.1 variant, His tag (28.78-0.45 nM). Coated protein was probed with the specified SARS-CoV-2 (COVID-19) Spike antibodies (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) or goat anti-mouse IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.](http://images.novusbio.com/fullsize/nbp3-25671_rabbit-sars-cov-2-spike-rbd-mab-hl1866-omicron-variant-azide-bsa-free-212024939162.jpg "ELISA: SARS-CoV-2 Spike RBD Antibody (HL1866) - Omicron Variant - Azide and BSA Free [NBP3-25671] - Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BQ.1 variant, His tag (28.78-0.45 nM). Coated protein was probed with the specified SARS-CoV-2 (COVID-19) Spike antibodies (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) or goat anti-mouse IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.")
![ELISA: SARS-CoV-2 Spike RBD Antibody (HL1866) - Omicron Variant - Azide and BSA Free [NBP3-25671] - Indirect ELISA analysis was performed by coating the plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.7 Alpha variant; B.1.351 Beta variant; P.1 Gamma variant; B1.617.2 Delta variant; BA.1 Omicron variant; BA.2 Omicron variant; BA.4/BA.5 Omicron variant) (28.78-0.45 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1866] (NBP3-25671) (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.](http://images.novusbio.com/fullsize/nbp3-25671_rabbit-sars-cov-2-spike-rbd-mab-hl1866-omicron-variant-azide-bsa-free-212024934276.jpg)
![Functional (Inhibition): SARS-CoV-2 Spike RBD Antibody (HL1866) - Omicron Variant - Azide and BSA Free [NBP3-25671] - Inhibition analysis of immobilized recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.1 Omicron variant; BA.2 Omicron variant; BA.4/BA.5 Omicron variant) (coated at 2 ug/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (1000 ng/mL) binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1866] (NBP3-25671) (0.09-66.67 nM). Bound was detected by Goat Anti-Mouse IgG antibody (HRP) (1:10000).](http://images.novusbio.com/fullsize/nbp3-25671_rabbit-sars-cov-2-spike-rbd-mab-hl1866-omicron-variant-azide-bsa-free-2120249432894.jpg)
![ELISA: SARS-CoV-2 Spike RBD Antibody (HL1866) - Omicron Variant - Azide and BSA Free [NBP3-25671] - Sandwich ELISA detection of recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.4/ BA.5 Omicron variant; Omicron / XBB variant) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (5 ug/mL) Detection: HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1866] (NBP3-25671) (1 ug/mL) Please notice that NBP3-25671 needs to be conjugated to HRP to function as the detection antibody when paired with HL1014. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/nbp3-25671_rabbit-sars-cov-2-spike-rbd-mab-hl1866-omicron-variant-azide-bsa-free-21202494089.jpg)
![ELISA: SARS-CoV-2 Spike RBD Antibody (HL1866) - Omicron Variant - Azide and BSA Free [NBP3-25671] - Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BF.7 variant, His tag (28.78-0.45 nM). Coated protein was probed with the specified SARS-CoV-2 (COVID-19) Spike antibodies (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) or goat anti-mouse IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.](http://images.novusbio.com/fullsize/nbp3-25671_rabbit-sars-cov-2-spike-rbd-mab-hl1866-omicron-variant-azide-bsa-free-2120249413381.jpg)
![ELISA: SARS-CoV-2 Spike RBD Antibody (HL1866) - Omicron Variant - Azide and BSA Free [NBP3-25671] - Sandwich ELISA detection of recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., BA.4/BA.5 Omicron variant; BF.7 Omicron variant; BQ.1 Omicron variant) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (5 ug/mL) Detection: HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1866] (NBP3-25671) (1 ug/mL) Please notice that NBP3-25671 needs to be conjugated to HRP to function as the detection antibody when paired with HL1014. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/nbp3-25671_rabbit-sars-cov-2-spike-rbd-mab-hl1866-omicron-variant-azide-bsa-free-2120249371599.jpg)
![ELISA: SARS-CoV-2 Spike RBD Antibody (HL1866) - Omicron Variant - Azide and BSA Free [NBP3-25671] - Sandwich ELISA detection of SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.2.75 variant, His tag using ELISA pairs below. Pair 1: Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (5 ug/mL) Detection: SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1866] (NBP3-25671) (1 ug/mL) Pair 2: Capture: SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1867] (NBP3-25672) (5 ug/mL) Detection: SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1866] (NBP3-25671) (1 ug/mL) Pair 3: Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (5 ug/mL) Detection: SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1867] (NBP3-25672) (1 ug/mL) Please notice that NBP3-25671 and NBP3-25672 needs to be conjugated to HRP to function as the detection antibody when paired with HL1014 and NBP3-25672. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/nbp3-25671_rabbit-sars-cov-2-spike-rbd-mab-hl1866-omicron-variant-azide-bsa-free-2120249413317.jpg)
![ELISA: SARS-CoV-2 Spike RBD Antibody (HL1866) - Omicron Variant - Azide and BSA Free [NBP3-25671] - Sandwich ELISA detection of SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.2.75 variant, His tag using ELISA pairs below. Pair 1: Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (5 ug/mL) Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (HRP) (HL1014-01) (1 ug/mL) Pair 2: Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (5 ug/mL) Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (HRP) (HL1014-01) (1 ug/mL) Pair 3: Capture: SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1866] (NBP3-25671) (5 ug/mL) Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (HRP) (HL1014-01) (1 ug/mL)](http://images.novusbio.com/fullsize/nbp3-25671_rabbit-sars-cov-2-spike-rbd-mab-hl1866-omicron-variant-azide-bsa-free-2120249342714.jpg)
![ELISA: SARS-CoV-2 Spike RBD Antibody (HL1866) - Omicron Variant - Azide and BSA Free [NBP3-25671] - Indirect ELISA analysis was performed by coating the plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., BA.2.12.1 Omicron variant; BA.4/BA.5 Omicron variant) (28.78-0.45 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1866] (NBP3-25671) (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.](http://images.novusbio.com/fullsize/nbp3-25671_rabbit-sars-cov-2-spike-rbd-mab-hl1866-omicron-variant-azide-bsa-free-2120249374921.jpg)
![Immunocytochemistry/Immunofluorescence: SARS-CoV-2 Spike RBD Antibody (HL1866) - Omicron Variant - Azide and BSA Free [NBP3-25671] - SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1866] detects SARS-CoV-2 (COVID-19) Spike RBD Omicron protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SARS-CoV-2 (COVID-19) Spike RBD Omicron stained by SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1866] (NBP3-25671) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25671_rabbit-sars-cov-2-spike-rbd-mab-hl1866-omicron-variant-azide-bsa-free-2120249362729.jpg)
![ELISA: SARS-CoV-2 Spike RBD Antibody (HL1867) - Omicron Variant - Azide and BSA Free [NBP3-25672] - Sandwich ELISA detection of SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.2.75 variant, His tag using ELISA pairs below. Pair 1: Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (5 ug/mL) Detection: SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1866] (NBP3-25671) (1 ug/mL) Pair 2: Capture: SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1867] (NBP3-25672) (5 ug/mL) Detection: SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1866] (NBP3-25671) (1 ug/mL) Pair 3: Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (5 ug/mL) Detection: SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1867] (NBP3-25672) (1 ug/mL) Please notice that NBP3-25671 and NBP3-25672 needs to be conjugated to HRP to function as the detection antibody when paired with HL1014 and NBP3-25672. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/nbp3-25672_rabbit-sars-cov-2-spike-rbd-mab-hl1867-omicron-variant-azide-bsa-free-2120249413310.jpg "ELISA: SARS-CoV-2 Spike RBD Antibody (HL1867) - Omicron Variant - Azide and BSA Free [NBP3-25672] - Sandwich ELISA detection of SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.2.75 variant, His tag using ELISA pairs below. Pair 1: Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (5 ug/mL) Detection: SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1866] (NBP3-25671) (1 ug/mL) Pair 2: Capture: SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1867] (NBP3-25672) (5 ug/mL) Detection: SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1866] (NBP3-25671) (1 ug/mL) Pair 3: Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (5 ug/mL) Detection: SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1867] (NBP3-25672) (1 ug/mL) Please notice that NBP3-25671 and NBP3-25672 needs to be conjugated to HRP to function as the detection antibody when paired with HL1014 and NBP3-25672. Please contact us for custom HRP-conjugated antibody.")
![ELISA: SARS-CoV-2 Spike RBD Antibody (HL1867) - Omicron Variant - Azide and BSA Free [NBP3-25672] - Indirect ELISA analysis was performed by coating the plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.7 Alpha variant; B.1.351 Beta variant; P.1 Gamma variant; B1.617.2 Delta variant; BA.1 Omicron variant; BA.2 Omicron variant; BA.4/BA.5 Omicron variant) (28.78-0.45 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1867] (NBP3-25672) (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.](http://images.novusbio.com/fullsize/nbp3-25672_rabbit-sars-cov-2-spike-rbd-mab-hl1867-omicron-variant-azide-bsa-free-212024940381.jpg)
![ELISA: SARS-CoV-2 Spike RBD Antibody (HL1867) - Omicron Variant - Azide and BSA Free [NBP3-25672] - Inhibition analysis of SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1867] (NBP3-25672) (104-13333 pM) using SARS-CoV-2 Neutralizing Antibody ELISA Kit (Omicron BA.1 / BA.2 / BA.4 / BA.5).](http://images.novusbio.com/fullsize/nbp3-25672_rabbit-sars-cov-2-spike-rbd-mab-hl1867-omicron-variant-azide-bsa-free-212024942542.jpg)
![ELISA: SARS-CoV-2 Spike RBD Antibody (HL1867) - Omicron Variant - Azide and BSA Free [NBP3-25672] - Sandwich ELISA detection of SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.2.75 variant, His tag using ELISA pairs below. Pair 1: Capture: SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1867] (NBP3-25672) (5 ug/mL) Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (HRP) (HL1014-01) (1 ug/mL) Pair 2: Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (5 ug/mL) Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (HRP) (1 ug/mL)](http://images.novusbio.com/fullsize/nbp3-25672_rabbit-sars-cov-2-spike-rbd-mab-hl1867-omicron-variant-azide-bsa-free-2120249351385.jpg)
![ELISA: SARS-CoV-2 Spike RBD Antibody (HL1867) - Omicron Variant - Azide and BSA Free [NBP3-25672] - Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BF.7 variant, His tag using antibodies as below. Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (5 ug/mL) Detection: HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1867] (NBP3-25672) (1 ug/mL) Please notice that NBP3-25672 needs to be conjugated to HRP to function as the detection antibody when paired with HL1014. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/nbp3-25672_rabbit-sars-cov-2-spike-rbd-mab-hl1867-omicron-variant-azide-bsa-free-2120249355581.jpg)
![Functional (Inhibition): SARS-CoV-2 Spike RBD Antibody (HL1867) - Omicron Variant - Azide and BSA Free [NBP3-25672] - Inhibition analysis of immobilized recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.1 Omicron variant; BA.2 Omicron variant; BA.4/BA.5 Omicron variant) (coated at 2 ug/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (1000 ng/mL) binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1867] (NBP3-25672) (0.09-66.67 nM). Bound was detected by Goat Anti-Mouse IgG antibody (HRP) (1:10000).](http://images.novusbio.com/fullsize/nbp3-25672_rabbit-sars-cov-2-spike-rbd-mab-hl1867-omicron-variant-azide-bsa-free-2120249374973.jpg)
![ELISA: SARS-CoV-2 Spike RBD Antibody (HL1867) - Omicron Variant - Azide and BSA Free [NBP3-25672] - Sandwich ELISA detection of recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., BA.4/BA.5 Omicron variant; BF.7 Omicron variant; BQ.1 Omicron variant) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (5 ug/mL) Detection: HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1867] (NBP3-25672) (1 ug/mL) Please notice that NBP3-25672 needs to be conjugated to HRP to function as the detection antibody when paired with HL1014. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/nbp3-25672_rabbit-sars-cov-2-spike-rbd-mab-hl1867-omicron-variant-azide-bsa-free-2120249425449.jpg)
![ELISA: SARS-CoV-2 Spike RBD Antibody (HL1867) - Omicron Variant - Azide and BSA Free [NBP3-25672] - Sandwich ELISA detection of recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.4/BA.5 Omicron variant) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (5 ug/mL) Detection: HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1867] (NBP3-25672) (1 ug/mL) Please notice that NBP3-25672 needs to be conjugated to HRP to function as the detection antibody when paired with HL1014. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/nbp3-25672_rabbit-sars-cov-2-spike-rbd-mab-hl1867-omicron-variant-azide-bsa-free-212024941338.jpg)
![ELISA: SARS-CoV-2 Spike RBD Antibody (HL1867) - Omicron Variant - Azide and BSA Free [NBP3-25672] - Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BF.7 variant, His tag (28.78-0.45 nM). Coated protein was probed with the specified SARS-CoV-2 (COVID-19) Spike antibodies (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) or goat anti-mouse IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.](http://images.novusbio.com/fullsize/nbp3-25672_rabbit-sars-cov-2-spike-rbd-mab-hl1867-omicron-variant-azide-bsa-free-2120249415955.jpg)
![Immunocytochemistry/Immunofluorescence: SARS-CoV-2 Spike RBD Antibody (HL1867) - Omicron Variant - Azide and BSA Free [NBP3-25672] - SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1867] detects SARS-CoV-2 (COVID-19) Spike RBD Omicron protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SARS-CoV-2 (COVID-19) Spike RBD Omicron stained by SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1867] (NBP3-25672) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25672_rabbit-sars-cov-2-spike-rbd-mab-hl1867-omicron-variant-azide-bsa-free-2120249374937.jpg)
![ELISA: SARS-CoV-2 Spike RBD Antibody (HL1867) - Omicron Variant - Azide and BSA Free [NBP3-25672] - Indirect ELISA analysis was performed by coating the plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., BA.2.12.1 Omicron variant; BA.4/BA.5 Omicron variant) (28.78-0.45 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1867] (NBP3-25672) (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.](http://images.novusbio.com/fullsize/nbp3-25672_rabbit-sars-cov-2-spike-rbd-mab-hl1867-omicron-variant-azide-bsa-free-2120249393569.jpg)
![ELISA: SARS-CoV-2 Spike RBD Antibody (HL1867) - Omicron Variant - Azide and BSA Free [NBP3-25672] - Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BQ.1 variant, His tag (28.78-0.45 nM). Coated protein was probed with the specified SARS-CoV-2 (COVID-19) Spike antibodies (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) or goat anti-mouse IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.](http://images.novusbio.com/fullsize/nbp3-25672_rabbit-sars-cov-2-spike-rbd-mab-hl1867-omicron-variant-azide-bsa-free-212024940811.jpg)
![Immunohistochemistry: SARS-CoV-2 Spike RBD Antibody (2165) [NBP3-07066] - SARS-CoV-2 Spike RBD Antibody (clone 2165) IHC data using infected cell pellets.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-RBD-Antibody-2165-Immunohistochemistry-NBP3-07066-img0003.jpg "Immunohistochemistry: SARS-CoV-2 Spike RBD Antibody (2165) [NBP3-07066] - SARS-CoV-2 Spike RBD Antibody (clone 2165) IHC data using infected cell pellets.")
![Neutralization: SARS-CoV-2 Spike RBD Antibody (2165) [NBP3-07066] - SARS-CoV-2 Spike RBD Antibody to the Receptor Binding Domain (RBD) (Clone 2165). Antibody binds to RBD and blocks binding to the ACE2 receptor on coated plates using a neutralization ELISA assay.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-RBD-Antibody-2165-Neutralization-NBP3-07066-img0002.jpg)
![Neutralization: SARS-CoV-2 Spike RBD Antibody (2165) [NBP3-07066] - Coating: Purified Recombinant SARS-CoV-2 Spike RBD, concentration of 1 ul/well, 100 ul/well overnight 2-8C. Detection: SARS-CoV-2 Spike RBD (Clone 2165) conjugated to HRP was serially diluted starting at 25 ng/ml down to 0.23 ng/ml, 100 ul/well for 1 hour at 37C. Substrate: TMB, 100 ul/well for 15 min. at room temperature followed by 450 nm stop solution, 50ul/well.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-RBD-Antibody-2165-Neutralization-NBP3-07066-img0001.jpg)
![Western Blot: SARS-CoV-2 Spike RBD Antibody (B001N) - (RBD) [NBP3-05728] - ELISA Coating original concentration: 2 ug/mL, 100 uL/well samples are column 1: SARS-CoV-2 Spike-ECD Recombinant Protein, and column 2: SARS-CoV-2 Spike-RBD Recombinant Protein, 100 uL/well. Stop buffer 50 uL/well.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-RBD-Antibody-B001N-RBD-Western-Blot-NBP3-05728-img0001.jpg "Western Blot: SARS-CoV-2 Spike RBD Antibody (B001N) - (RBD) [NBP3-05728] - ELISA Coating original concentration: 2 ug/mL, 100 uL/well samples are column 1: SARS-CoV-2 Spike-ECD Recombinant Protein, and column 2: SARS-CoV-2 Spike-RBD Recombinant Protein, 100 uL/well. Stop buffer 50 uL/well.")
![ELISA: SARS-CoV-2 Spike RBD Antibody (B001N) - (RBD) [NBP3-05728] - ELISA Test. Coating original concentration: 2 ug/mL, 100 uL/well samples are column 1: SARS-CoV-2 (COVID-19) Spike-ECD Recombinant Protein, and column 2: SARS-CoV-2 (COVID-19) Spike-RBD Recombinant Protein. Antibodies: SARS-CoV-2 Spike RBD Antibody, NBP3-05728. Secondary: Goat anti-human IgG HRP conjugate at 1:10000 dilution. Develop: 15min, 100 uL/well. Stop: Stop buffer 50 uL/well.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-RBD-Antibody-B001N-RBD-ELISA-NBP3-05728-img0002.jpg)
![Western Blot: SARS-CoV-2 Spike RBD Antibody (B003N) - (RBD) [NBP3-05727] - ELISA Coating original concentration: 2 ug/mL, 100 uL/well samples are column 1: SARS-CoV-2 Spike-ECD Recombinant Protein, and column 2: SARS-CoV-2 Spike-RBD Recombinant Protein, 100 uL/well. Stop buffer 50 uL/well.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-RBD-Antibody-B003N-RBD-Western-Blot-NBP3-05727-img0001.jpg "Western Blot: SARS-CoV-2 Spike RBD Antibody (B003N) - (RBD) [NBP3-05727] - ELISA Coating original concentration: 2 ug/mL, 100 uL/well samples are column 1: SARS-CoV-2 Spike-ECD Recombinant Protein, and column 2: SARS-CoV-2 Spike-RBD Recombinant Protein, 100 uL/well. Stop buffer 50 uL/well.")
![ELISA: SARS-CoV-2 Spike RBD Antibody (B003N) - (RBD) [NBP3-05727] - ELISA Test. Coating original concentration: 2 ug/mL, 100 uL/well samples are column 1: SARS-CoV-2 (COVID-19) Spike-ECD Recombinant Protein, and column 2: SARS-CoV-2 (COVID-19) Spike-RBD Recombinant Protein. Antibodies: SARS-CoV-2 Spike RBD Antibody, NBP3-05727. Secondary: Goat anti-human IgG HRP conjugate at 1:10000 dilution. Develop: 15min, 100 uL/well. Stop: Stop buffer 50 uL/well.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-RBD-Antibody-B003N-RBD-ELISA-NBP3-05727-img0002.jpg)
![Western Blot: SARS-CoV-2 Spike RBD Antibody (B001Y) - (RBD) [NBP3-05725] - ELISA Coating original concentration: 2 ug/mL, 100 uL/well samples are column 1: SARS-CoV-2 Spike-ECD Recombinant Protein, and column 2: SARS-CoV-2 Spike-RBD Recombinant Protein, 100 uL/well. Stop buffer 50 uL/well.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-RBD-Antibody-B001Y-RBD-Western-Blot-NBP3-05725-img0001.jpg "Western Blot: SARS-CoV-2 Spike RBD Antibody (B001Y) - (RBD) [NBP3-05725] - ELISA Coating original concentration: 2 ug/mL, 100 uL/well samples are column 1: SARS-CoV-2 Spike-ECD Recombinant Protein, and column 2: SARS-CoV-2 Spike-RBD Recombinant Protein, 100 uL/well. Stop buffer 50 uL/well.")
![ELISA: SARS-CoV-2 Spike RBD Antibody (B001Y) - (RBD) [NBP3-05725] - ELISA Test. Coating original concentration: 2 ug/mL, 100 uL/well samples are column 1: SARS-CoV-2 (COVID-19) Spike-ECD Recombinant Protein and column 2: SARS-CoV-2 (COVID-19) Spike-RBD Recombinant Protein. Antibodies: SARS-CoV-2 Spike RBD Antibody, NBP3-05725. Secondary: Goat anti-human IgG HRP conjugate at 1:10000 dilution. Develop: 15min, 100 uL/well. Stop: Stop buffer 50 uL/well.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-RBD-Antibody-B001Y-RBD-ELISA-NBP3-05725-img0002.jpg)
![Western Blot: SARS-CoV-2 Spike RBD Antibody (B003Y) - (RBD) [NBP3-05726] - ELISA Coating original concentration: 2 ug/mL, 100 uL/well samples are column 1: SARS-CoV-2 Spike-ECD Recombinant Protein, and column 2: SARS-CoV-2 Spike-RBD Recombinant Protein, 100 uL/well. Stop buffer 50 uL/well.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-RBD-Antibody-B003Y-RBD-Western-Blot-NBP3-05726-img0001.jpg "Western Blot: SARS-CoV-2 Spike RBD Antibody (B003Y) - (RBD) [NBP3-05726] - ELISA Coating original concentration: 2 ug/mL, 100 uL/well samples are column 1: SARS-CoV-2 Spike-ECD Recombinant Protein, and column 2: SARS-CoV-2 Spike-RBD Recombinant Protein, 100 uL/well. Stop buffer 50 uL/well.")
![ELISA: SARS-CoV-2 Spike RBD Antibody (B003Y) - (RBD) [NBP3-05726] - ELISA Test. Coating original concentration: 2 ug/mL, 100 uL/well samples are column 1: SARS-CoV-2 (COVID-19) Spike-ECD Recombinant Protein, and column 2: SARS-CoV-2 (COVID-19) Spike-RBD Recombinant Protein. Antibodies: SARS-CoV-2 Spike RBD Antibody, NBP3-05726. Secondary: Goat anti-human IgG HRP conjugate at 1:10000 dilution. Develop: 15min, 100 uL/well. Stop: Stop buffer 50 uL/well.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-RBD-Antibody-B003Y-RBD-ELISA-NBP3-05726-img0002.jpg)
![ELISA: SARS-CoV-2 Spike RBD Antibody (T4P3-B5) - VHH [NBP3-12841] - A direct ELISA was performed using SARS-CoV-2 Spike RBD recombinant protein as coating antigen at 1 ug/mL and the anti-SARS-CoV-2 Spike RBD antibody (NBP3-12841) as the capture antibody, following by anti-cMyc-tag antibody at 1 ug/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL. SARS-CoV-2 Spike RBD Antibody, NBP3-12841 can detect spike RBD protein at 10 ng/mL.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-RBD-Antibody-T4P3-B5-VHH-ELISA-NBP3-12841-img0001.jpg "ELISA: SARS-CoV-2 Spike RBD Antibody (T4P3-B5) - VHH [NBP3-12841] - A direct ELISA was performed using SARS-CoV-2 Spike RBD recombinant protein as coating antigen at 1 ug/mL and the anti-SARS-CoV-2 Spike RBD antibody (NBP3-12841) as the capture antibody, following by anti-cMyc-tag antibody at 1 ug/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL. SARS-CoV-2 Spike RBD Antibody, NBP3-12841 can detect spike RBD protein at 10 ng/mL.")
![ELISA: SARS-CoV-2 Spike RBD Antibody (T5P4-A12) - VHH [NBP3-12844] - A direct ELISA was performed using SARS-CoV-2 Spike recombinant proteins (RBD, S1, ECD) as coating antigen at 1 ug/mL and the anti-SARS-CoV-2 Spike RBD antibody (NBP3-12844) as the capture antibody, following by anti-cMyc-tag antibody at 1 ug/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL. SARS-CoV-2 Spike RBD Antibody, NBP3-12844 can detect spike RBD, S1 and ECD proteins at 2 ng/mL.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-RBD-Antibody-T5P4-A12-VHH-ELISA-NBP3-12844-img0001.jpg "ELISA: SARS-CoV-2 Spike RBD Antibody (T5P4-A12) - VHH [NBP3-12844] - A direct ELISA was performed using SARS-CoV-2 Spike recombinant proteins (RBD, S1, ECD) as coating antigen at 1 ug/mL and the anti-SARS-CoV-2 Spike RBD antibody (NBP3-12844) as the capture antibody, following by anti-cMyc-tag antibody at 1 ug/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL. SARS-CoV-2 Spike RBD Antibody, NBP3-12844 can detect spike RBD, S1 and ECD proteins at 2 ng/mL.")
![ELISA: SARS-CoV-2 Spike RBD Antibody (T5P7-G12) - VHH [NBP3-12847] - A direct ELISA was performed using SARS-CoV-2 Spike RBD recombinant protein as coating antigen at 1 ug/mL and the anti-SARS-CoV-2 Spike RBD antibody (NBP3-12847) as the capture antibody, following by anti-cMyc-tag antibody at 1 ug/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL. SARS-CoV-2 Spike RBD Antibody, NBP3-12847 can detect spike RBD protein at 20 ng/mL.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-RBD-Antibody-T5P7-G12-VHH-ELISA-NBP3-12847-img0001.jpg "ELISA: SARS-CoV-2 Spike RBD Antibody (T5P7-G12) - VHH [NBP3-12847] - A direct ELISA was performed using SARS-CoV-2 Spike RBD recombinant protein as coating antigen at 1 ug/mL and the anti-SARS-CoV-2 Spike RBD antibody (NBP3-12847) as the capture antibody, following by anti-cMyc-tag antibody at 1 ug/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL. SARS-CoV-2 Spike RBD Antibody, NBP3-12847 can detect spike RBD protein at 20 ng/mL.")
![ELISA: SARS-CoV-2 Spike RBD Antibody (T3P1-C8) - VHH [NBP3-12849] - A direct ELISA was performed using SARS-CoV-2 Spike recombinant proteins (RBD, S1, ECD) as coating antigen at 1 ug/mL and the anti-SARS-CoV-2 Spike RBD antibody (NBP3-12849) as the capture antibody, following by anti-cMyc-tag antibody at 1 ug/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL. SARS-CoV-2 Spike RBD Antibody, NBP3-12849 can detect spike RBD, S1 and ECD proteins at 2 ng/mL.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-RBD-Antibody-T3P1-C8-VHH-ELISA-NBP3-12849-img0001.jpg "ELISA: SARS-CoV-2 Spike RBD Antibody (T3P1-C8) - VHH [NBP3-12849] - A direct ELISA was performed using SARS-CoV-2 Spike recombinant proteins (RBD, S1, ECD) as coating antigen at 1 ug/mL and the anti-SARS-CoV-2 Spike RBD antibody (NBP3-12849) as the capture antibody, following by anti-cMyc-tag antibody at 1 ug/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL. SARS-CoV-2 Spike RBD Antibody, NBP3-12849 can detect spike RBD, S1 and ECD proteins at 2 ng/mL.")