Note: Mouseover a species abbreviation on the product page to display the fullname.
How can we help you?
![Western Blot: Ribosomal Protein S6/RPS6 Antibody [NB100-1595] - Detection of human and mouse RPS6 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPS6 antibody NB100-1595 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.Previous](http://images.novusbio.com/fullsize/Ribosomal-Protein-S6-RPS6-Antibody-Western-Blot-NB100-1595-img0007.jpg "Western Blot: Ribosomal Protein S6/RPS6 Antibody [NB100-1595] - Detection of human and mouse RPS6 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPS6 antibody NB100-1595 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.Previous")
![Immunohistochemistry-Paraffin: Ribosomal Protein S6/RPS6 Antibody [NB100-1595] - Section of human lung carcinoma. Antibody: Affinity purified rabbit anti-RPS6 (NB100-1595). Detection: DAB](http://images.novusbio.com/fullsize/Ribosomal-Protein-S6-RPS6-Antibody-Immunohistochemistry-NB100-1595-img0009.jpg)
![Immunoprecipitation: Ribosomal Protein S6/RPS6 Antibody [NB100-1595] - Detection of human RPS6 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RPS6 antibody NB100-1595 (lot NB100-1595-2) used for IP at 6 ug per reaction. RPS6 was also immunoprecipitated by a previous lot of this antibody (lot NB100-1595-1). For blotting immunoprecipitated RPS6, NB100-1595 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/Ribosomal-Protein-S6-RPS6-Antibody-Immunoprecipitation-NB100-1595-img0008.jpg)
![ALM inhibits HIF-1 alpha translation by down-regulating mTOR pathway. (A) Hep3B and PC3 cells were exposed to vehicle or the hypoxia mimics dimethyloxalylglycine (DMOG), cobalt chloride (CoCl2), desferrioxamine (DFX) or MG132 in the presence of ALM at indicated concentrations or vehicle for 24 h and whole cell lysates were subjected to Western blot. (B) Hep3B cells were pretreated for 4 h with vehicle (Vhc), 25 nM rapamycin (Rapa), 20 μg/mL cycloheximide (Chx), or 200 nM ALM, [35S] methionine/cysteine was added for 1 h followed by cell lysis, HIF-1 alpha immunoprecipitation (IP), SDS/PAGE, and autoradiography. (C–E) PC3 cells were cultured at 20% or 1% O2 for 24 h in the presence of ALM (at the indicated concentrations) and whole cell lysates were subjected to for: (C) p-P70, p-Akt, p-mTOR (2448/2481), mTOR, (D) p-4E-BP1, (E) p-RPS6, HIF-1 alpha , or beta -actin. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31083403), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/af3918_phospho-rps6-s235-s236-affinity-purified-polyclonal-ab-41202412403591.jpg)
![Immunohistochemistry-Paraffin: Ribosomal Protein S6/RPS6 [p Ser235, p Ser236] Antibody (SP50) [NBP2-12478] - Human Thymus stained with anti-S6 Ribosomal (Phospho S235/236) antibody.](http://images.novusbio.com/fullsize/Ribosomal-Protein-S6-RPS6-p-Ser235-p-Ser236-Antibody-SP50-Immunohistochemistry-Paraffin-NBP2-12478-img0011.jpg "Immunohistochemistry-Paraffin: Ribosomal Protein S6/RPS6 [p Ser235, p Ser236] Antibody (SP50) [NBP2-12478] - Human Thymus stained with anti-S6 Ribosomal (Phospho S235/236) antibody.")
![Immunohistochemistry-Paraffin: Ribosomal Protein S6/RPS6 [p Ser235, p Ser236] Antibody (SP50) [NBP2-12478] - Human Breast Carcinoma stained with anti-S6 Ribosomal (Phospho S235/236) antibody.](http://images.novusbio.com/fullsize/Ribosomal-Protein-S6-RPS6-p-Ser235-p-Ser236-Antibody-SP50-Immunohistochemistry-Paraffin-NBP2-12478-img0001.jpg)
![Immunohistochemistry-Paraffin: Ribosomal Protein S6/RPS6 [p Ser235, p Ser236] Antibody (SP50) [NBP2-12478] - Human Breast Ductal Cell Carcinoma stained with anti-S6 Ribosomal (phospho S235/236) antibody.](http://images.novusbio.com/fullsize/Ribosomal-Protein-S6-RPS6-p-Ser235-p-Ser236-Antibody-SP50-Immunohistochemistry-Paraffin-NBP2-12478-img0002.jpg)
![Immunohistochemistry-Paraffin: Ribosomal Protein S6/RPS6 [p Ser235, p Ser236] Antibody (SP50) [NBP2-12478] - Human Bladder Transitional Cell Carcinoma stained with anti-S6 Ribosomal (Phospho S235/236)antibody.](http://images.novusbio.com/fullsize/Ribosomal-Protein-S6-RPS6-p-Ser235-p-Ser236-Antibody-SP50-Immunohistochemistry-Paraffin-NBP2-12478-img0003.jpg)
![Immunohistochemistry-Paraffin: Ribosomal Protein S6/RPS6 [p Ser235, p Ser236] Antibody (SP50) [NBP2-12478] - Human Colon Adenocarcinoma stained with anti-S6 Ribosomal (Phospho S235/236)antibody.](http://images.novusbio.com/fullsize/Ribosomal-Protein-S6-RPS6-p-Ser235-p-Ser236-Antibody-SP50-Immunohistochemistry-Paraffin-NBP2-12478-img0004.jpg)
![Immunohistochemistry-Paraffin: Ribosomal Protein S6/RPS6 [p Ser235, p Ser236] Antibody (SP50) [NBP2-12478] - Human Hepatocellular Cell Carcinoma stained with anti-S6 Ribosomal (Phospho S235/236)antibody.](http://images.novusbio.com/fullsize/Ribosomal-Protein-S6-RPS6-p-Ser235-p-Ser236-Antibody-SP50-Immunohistochemistry-Paraffin-NBP2-12478-img0005.jpg)
![Immunohistochemistry-Paraffin: Ribosomal Protein S6/RPS6 [p Ser235, p Ser236] Antibody (SP50) [NBP2-12478] - Human Lung Adenocarcinoma stained with anti-S6 Ribosomal (Phospho S235/236) antibody.](http://images.novusbio.com/fullsize/Ribosomal-Protein-S6-RPS6-p-Ser235-p-Ser236-Antibody-SP50-Immunohistochemistry-Paraffin-NBP2-12478-img0006.jpg)
![Immunohistochemistry-Paraffin: Ribosomal Protein S6/RPS6 [p Ser235, p Ser236] Antibody (SP50) [NBP2-12478] - Human Lung Squamous Cell Carcinoma stained with anti-S6 Ribosomal (Phospho S235/236) antibody.](http://images.novusbio.com/fullsize/Ribosomal-Protein-S6-RPS6-p-Ser235-p-Ser236-Antibody-SP50-Immunohistochemistry-Paraffin-NBP2-12478-img0007.jpg)
![Immunohistochemistry-Paraffin: Ribosomal Protein S6/RPS6 [p Ser235, p Ser236] Antibody (SP50) [NBP2-12478] - Human Renal Cell Carcinoma stained with anti-S6 Ribosomal (Phospho S235/236) antibody.](http://images.novusbio.com/fullsize/Ribosomal-Protein-S6-RPS6-p-Ser235-p-Ser236-Antibody-SP50-Immunohistochemistry-Paraffin-NBP2-12478-img0008.jpg)
![Immunohistochemistry-Paraffin: Ribosomal Protein S6/RPS6 [p Ser235, p Ser236] Antibody (SP50) [NBP2-12478] - Human Stomach Adenocarcinoma stained with anti-S6 Ribosomal (Phospho S235/236) antibody.](http://images.novusbio.com/fullsize/Ribosomal-Protein-S6-RPS6-p-Ser235-p-Ser236-Antibody-SP50-Immunohistochemistry-Paraffin-NBP2-12478-img0009.jpg)
![Immunohistochemistry-Paraffin: Ribosomal Protein S6/RPS6 [p Ser235, p Ser236] Antibody (SP50) [NBP2-12478] - Human Tonsil stained with anti-S6 Ribosomal (Phospho S235/236) antibody.](http://images.novusbio.com/fullsize/Ribosomal-Protein-S6-RPS6-p-Ser235-p-Ser236-Antibody-SP50-Immunohistochemistry-Paraffin-NBP2-12478-img0010.jpg)
![Western Blot: Ribosomal Protein S6/RPS6 Antibody [NBP1-87099] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: MOLT-4](http://images.novusbio.com/fullsize/Ribosomal-Protein-S6-RPS6-Antibody-Western-Blot-NBP1-87099-img0004.jpg "Western Blot: Ribosomal Protein S6/RPS6 Antibody [NBP1-87099] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: MOLT-4")
![Immunocytochemistry/Immunofluorescence: Ribosomal Protein S6/RPS6 Antibody [NBP1-87099] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol & endoplasmic reticulum.](http://images.novusbio.com/fullsize/Ribosomal-Protein-S6-RPS6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87099-img0006.jpg)
![Immunohistochemistry-Paraffin: Ribosomal Protein S6/RPS6 Antibody [NBP1-87099] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/Ribosomal-Protein-S6-RPS6-Antibody-Immunohistochemistry-Paraffin-NBP1-87099-img0005.jpg)
![Western Blot: Ribosomal Protein S6/RPS6 [p Ser235] Antibody [NBP3-13078] - Untreated (-) and treated (+) 293T whole cell extracts starvation for 16 hrs (40 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ribosomal Protein S6/RPS6 (phospho Ser235) antibody (NBP3-13078) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Ribosomal-Protein-S6-RPS6-[p-Ser235]-Antibody-Western-Blot-NBP3-13078-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: Ribosomal Protein S6/RPS6 [p Ser235] Antibody [NBP3-13078] - Untreated (-) and treated (+) 293T whole cell extracts starvation for 16 hrs (40 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ribosomal Protein S6/RPS6 (phospho Ser235) antibody (NBP3-13078) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Ribosomal Protein S6/RPS6 [p Ser235] Antibody [NBP3-13078] - Ribosomal Protein S6/RPS6 (phospho Ser235) antibody detects Ribosomal Protein S6/RPS6 (phospho Ser235) protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Ribosomal Protein S6/RPS6 (phospho Ser235) antibody (NBP3-13078) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Ribosomal-Protein-S6-RPS6-[p-Ser235]-Antibody-Immunohistochemistry-Paraffin-NBP3-13078-img0001.jpg)
![Western Blot: Ribosomal Protein S6/RPS6 [p Ser235, p Ser236] Antibody [NB100-1553] - Detection of Human Phospho RPS6 (S235/S236) by Western Blot. Samples: Whole cell lysate (35 mcg) from HeLa cells. Treatments consisted of growth in serum containing media (lane 1) or cultured for 24 hours in serum free media (lanes 2 to 5). At 24h, serum was replaced for 1 h (lanes 2 and 3) or serum starvation was continued (lanes 4 and 5) without rapamycin (lanes 3 and 5) with 50nM Rapamcycin (lanes 2 and 4). Antibodies: Affinity purified rabbit anti-phospho RPS6 (S235/S236) antibody NB100-1553 used at 0.04 mcg/ml (upper panel). The blot was stripped and re-blotted for total RPS6 using affinity purified rabbit anti-RPS6 antibody NB100-1595 at 0.04 mcg/ml. Detection: Chemiluminescence with exposure times of 10 seconds (upper panel) or 30 seconds (lower panel).](http://images.novusbio.com/fullsize/Ribosomal-Protein-S6-RPS6-[p-Ser235--p-Ser236]-Antibody-Western-Blot-NB100-1553-img0002.jpg "Western Blot: Ribosomal Protein S6/RPS6 [p Ser235, p Ser236] Antibody [NB100-1553] - Detection of Human Phospho RPS6 (S235/S236) by Western Blot. Samples: Whole cell lysate (35 mcg) from HeLa cells. Treatments consisted of growth in serum containing media (lane 1) or cultured for 24 hours in serum free media (lanes 2 to 5). At 24h, serum was replaced for 1 h (lanes 2 and 3) or serum starvation was continued (lanes 4 and 5) without rapamycin (lanes 3 and 5) with 50nM Rapamcycin (lanes 2 and 4). Antibodies: Affinity purified rabbit anti-phospho RPS6 (S235/S236) antibody NB100-1553 used at 0.04 mcg/ml (upper panel). The blot was stripped and re-blotted for total RPS6 using affinity purified rabbit anti-RPS6 antibody NB100-1595 at 0.04 mcg/ml. Detection: Chemiluminescence with exposure times of 10 seconds (upper panel) or 30 seconds (lower panel).")