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![Western Blot: QKI/Quaking Antibody [NBP1-87178] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-Western-Blot-NBP1-87178-img0017.jpg "Western Blot: QKI/Quaking Antibody [NBP1-87178] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunocytochemistry/Immunofluorescence: QKI/Quaking Antibody [NBP1-87178] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87178-img0026.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: QKI/Quaking Antibody [NBP1-87178] - Staining in human cerebral cortex and pancreas tissues . Corresponding QKI RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-Immunohistochemistry-Paraffin-NBP1-87178-img0025.jpg)
![Western Blot: QKI/Quaking Antibody [NBP1-87178] - Analysis in human cerebral cortex tissue.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-Western-Blot-NBP1-87178-img0016.jpg)
![Immunohistochemistry: QKI/Quaking Antibody [NBP1-87178] - Staining of mouse brain shows strong positivity in a subset of glial cells in the cerebral cortex.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-Immunohistochemistry-Paraffin-NBP1-87178-img0021.jpg)
![Immunohistochemistry-Paraffin: QKI/Quaking Antibody [NBP1-87178] - Staining of human cerebellum shows moderate to strong nuclear positivity in glial cells.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-Immunohistochemistry-Paraffin-NBP1-87178-img0022.jpg)
![Immunohistochemistry-Paraffin: QKI/Quaking Antibody [NBP1-87178] - Staining of human cerebral cortex shows moderate to strong nuclear positivity in glial cells.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-Immunohistochemistry-Paraffin-NBP1-87178-img0023.jpg)
![Immunohistochemistry-Paraffin: QKI/Quaking Antibody [NBP1-87178] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-Immunohistochemistry-Paraffin-NBP1-87178-img0024.jpg)
![Western Blot: QKI/Quaking Antibody [NBP2-20029] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with QKI antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-Western-Blot-NBP2-20029-img0007.jpg "Western Blot: QKI/Quaking Antibody [NBP2-20029] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with QKI antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: QKI/Quaking Antibody [NBP2-20029] - QKI antibody detects QKI protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: QKI protein stained by QKI antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20029-img0004.jpg)
![Immunohistochemistry-Frozen: QKI/Quaking Antibody [NBP2-20029] - QKI antibody detects QKI protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: QKI protein stained by QKI antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-Immunohistochemistry-Frozen-NBP2-20029-img0006.jpg)
![Western Blot: QKI/Quaking Antibody [NBP2-20029] - Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-Western-Blot-NBP2-20029-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: QKI/Quaking Antibody [NBP2-20029] - Immunofluorescence analysis of paraformaldehyde-fixed A431, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20029-img0003.jpg)
![Immunohistochemistry-Paraffin: QKI/Quaking Antibody [NBP2-20029] - Immunohistochemical analysis of paraffin-embedded MDA-MB157 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-Immunohistochemistry-Paraffin-NBP2-20029-img0002.jpg)
![Immunohistochemistry-Frozen: QKI/Quaking Antibody [NBP2-20029] - QKI antibody detects QKI protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: QKI protein stained by QKI antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-Immunohistochemistry-Frozen-NBP2-20029-img0005.jpg)
![Western Blot: QKI/Quaking Antibody [NB300-240] - Detection of Human and Mouse QKI by Western Blot. Samples: Whole cell lysate from HeLa (15 and 50 ug), 293T (50ug), and mouse NIH3T3 (50ug) cells. Antibodies: Affinity purified rabbit anti-QKI antibody NB300-240 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-Western-Blot-NB300-240-img0008.jpg "<b>Biological Strategies Validation. </b>Western Blot: QKI/Quaking Antibody [NB300-240] - Detection of Human and Mouse QKI by Western Blot. Samples: Whole cell lysate from HeLa (15 and 50 ug), 293T (50ug), and mouse NIH3T3 (50ug) cells. Antibodies: Affinity purified rabbit anti-QKI antibody NB300-240 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: QKI/Quaking Antibody [NB300-240] - Sample: FFPE sections of human testicular seminoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti- QKI used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-Immunohistochemistry-NB300-240-img0006.jpg)
![Immunoprecipitation: QKI/Quaking Antibody [NB300-240] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. antibodies: Affinity purified rabbit anti-QKI antibody used for IP at 6 ug/mg lysate. QKI was also immunoprecipitated by a prior lot of this antibody. For blotting immunoprecipitated QKI, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-Immunoprecipitation-NB300-240-img0011.jpg)
![Western Blot: QKI/Quaking Antibody (1D1N6) [NBP3-15255] - Western blot analysis of extracts of various cell lines, using QKI/Quaking antibody (NBP3-15255) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-1D1N6-Western-Blot-NBP3-15255-img0002.jpg "Western Blot: QKI/Quaking Antibody (1D1N6) [NBP3-15255] - Western blot analysis of extracts of various cell lines, using QKI/Quaking antibody (NBP3-15255) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: QKI/Quaking Antibody (1D1N6) [NBP3-15255] - Immunofluorescence analysis of NIH/3T3 cells using QKI/Quaking Rabbit mAb (NBP3-15255) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-1D1N6-Immunocytochemistry-Immunofluorescence-NBP3-15255-img0001.jpg)
![Immunoprecipitation: QKI/Quaking Antibody (1D1N6) [NBP3-15255] - Immunoprecipitation analysis of 300ug extracts of NIH/3T3 cells using 3ug QKI/Quaking antibody (NBP3-15255). Western blot was performed from the immunoprecipitate using QKI/Quaking antibody (NBP3-15255) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-1D1N6-Immunoprecipitation-NBP3-15255-img0003.jpg)
![Western Blot: QKI/Quaking Antibody [NB300-239] - Detection of Human QKI by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-QKI antibody NB300-239 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-Western-Blot-NB300-239-img0008.jpg "<b>Biological Strategies Validation. </b>Western Blot: QKI/Quaking Antibody [NB300-239] - Detection of Human QKI by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-QKI antibody NB300-239 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Western Blot: QKI/Quaking Antibody [NB300-239] - Nuclear extract (20 ug) from HeLa cells. Antibody used at 0.2 ug/ml.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-Western-Blot-NB300-239-img0002.jpg)
![Western Blot: QKI/Quaking Antibody (S01-3F7) [NBP3-19753] - Western blot detection of QKI/Quaking in K562 cell lysates using NBP3-19753 (1:1000 diluted). Predicted band size: 38kDa. Observed band size: 38kDa.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-S01-3F7-Western-Blot-NBP3-19753-img0001.jpg "Western Blot: QKI/Quaking Antibody (S01-3F7) [NBP3-19753] - Western blot detection of QKI/Quaking in K562 cell lysates using NBP3-19753 (1:1000 diluted). Predicted band size: 38kDa. Observed band size: 38kDa.")