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![Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody [NBP1-87246] - Staining in human testis and pancreas tissues using anti-PPM1G antibody. Corresponding PPM1G RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Immunohistochemistry-Paraffin-NBP1-87246-img0013.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody [NBP1-87246] - Staining in human testis and pancreas tissues using anti-PPM1G antibody. Corresponding PPM1G RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody [NBP1-87246] - Staining of human cerebral cortex, kidney, lymph node and testis using Anti-PPM1G antibody NBP1-87246 (A) shows similar protein distribution across tissues to independent antibody NBP1-87245 (B).](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Immunohistochemistry-Paraffin-NBP1-87246-img0016.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: PP2C gamma/PPM1G Antibody [NBP1-87246] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-PPM1G antibody. Remaining relative intensity is presented.](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Western-Blot-NBP1-87246-img0015.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: PP2C gamma/PPM1G Antibody [NBP1-87246] - Analysis using Anti-PPM1G antibody NBP1-87246 (A) shows similar pattern to independent antibody NBP1-87245 (B).](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Western-Blot-NBP1-87246-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: PP2C gamma/PPM1G Antibody [NBP1-87246] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87246-img0014.jpg)
![Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody [NBP1-87246] - Staining of human lymph node.](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Immunohistochemistry-Paraffin-NBP1-87246-img0019.jpg)
![Western Blot: PP2C gamma/PPM1G Antibody [NBP1-87246] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Western-Blot-NBP1-87246-img0009.jpg)
![Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody [NBP1-87246] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Immunohistochemistry-Paraffin-NBP1-87246-img0011.jpg)
![Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody [NBP1-87246] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Immunohistochemistry-Paraffin-NBP1-87246-img0012.jpg)
![Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody [NBP1-87246] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Immunohistochemistry-Paraffin-NBP1-87246-img0018.jpg)
![Western Blot: PP2C gamma/PPM1G Antibody [NBP1-87245] - Analysis using Anti-PPM1G antibody NBP1-87245 (A) shows similar pattern to independent antibody NBP1-87246 (B).](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Western-Blot-NBP1-87245-img0016.jpg "<b>Independent Antibodies Validation. </b>Western Blot: PP2C gamma/PPM1G Antibody [NBP1-87245] - Analysis using Anti-PPM1G antibody NBP1-87245 (A) shows similar pattern to independent antibody NBP1-87246 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: PP2C gamma/PPM1G Antibody [NBP1-87245] - Staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87245-img0014.jpg)
![Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody [NBP1-87245] - Staining of human lymph node.](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Immunohistochemistry-Paraffin-NBP1-87245-img0018.jpg)
![Western Blot: PP2C gamma/PPM1G Antibody [NBP1-87245] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Western-Blot-NBP1-87245-img0009.jpg)
![Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody [NBP1-87245] - Staining of human testis shows strong nuclear positivity in seminiferous tubules.](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Immunohistochemistry-Paraffin-NBP1-87245-img0008.jpg)
![Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody [NBP1-87245] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Immunohistochemistry-Paraffin-NBP1-87245-img0011.jpg)
![Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody [NBP1-87245] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Immunohistochemistry-Paraffin-NBP1-87245-img0012.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody [NBP1-87245] - Staining in human testis and pancreas tissues using anti-PPM1G antibody. Corresponding PPM1G RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Immunohistochemistry-Paraffin-NBP1-87245-img0013.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody [NBP1-87245] - Staining of human cerebral cortex, kidney, lymph node and testis using Anti-PPM1G antibody NBP1-87245 (A) shows similar protein distribution across tissues to independent antibody NBP1-87246 (B).](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Immunohistochemistry-Paraffin-NBP1-87245-img0015.jpg)
![Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody [NBP1-87245] - Staining of human kidney.](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Immunohistochemistry-Paraffin-NBP1-87245-img0017.jpg)
![Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody [NBP1-87245] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Immunohistochemistry-Paraffin-NBP1-87245-img0019.jpg)
![Western Blot: PP2C gamma/PPM1G Antibody [NB110-38867] - Detection of Human PPM1G on HeLa whole cell lysate (5, 15, and 50 microgram) and 293T (50 microgram) using NB110-38867. PPM1G was also immunoprecipitated by rabbit anti-PPM1G antibody NB110-38869.](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Western-Blot-NB110-38867-img0003.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: PP2C gamma/PPM1G Antibody [NB110-38867] - Detection of Human PPM1G on HeLa whole cell lysate (5, 15, and 50 microgram) and 293T (50 microgram) using NB110-38867. PPM1G was also immunoprecipitated by rabbit anti-PPM1G antibody NB110-38869.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry: PP2C gamma/PPM1G Antibody [NB110-38867] - Sample: FFPE sections of human skin basal cell carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti- PPM1G used at a dilution of 1:1,000 (0.2ug/ml) and 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Immunohistochemistry-NB110-38867-img0005.jpg)
![Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody [NB110-38867] - FFPE section of human breast adenocarcinoma. Affinity purified rabbit anti-PPM1G used at a dilution of 1:500.](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Immunohistochemistry-Paraffin-NB110-38867-img0001.jpg)
![Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody [NB110-38867] - FFPE section of mouse squamous cell carcinoma. Affinity purified rabbit anti-PPM1G used at a dilution of 1:100.](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Immunohistochemistry-Paraffin-NB110-38867-img0002.jpg)
![Immunohistochemistry: PP2C gamma/PPM1G Antibody [NB110-38867] - Sample: FFPE sections of human skin basal cell carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti- PPM1G used at a dilution of 1:1,000 (0.2ug/ml) and 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Immunohistochemistry-NB110-38867-img0004.jpg)
![Western Blot: PP2C gamma/PPM1G Antibody [NBP2-99758] - Anti-PP2C gamma/PPM1G rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: 292T Whole Cell Lysate. Lane C: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 59 kDa. Observed band size: 80 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Western-Blot-NBP2-99758-img0003.jpg "Western Blot: PP2C gamma/PPM1G Antibody [NBP2-99758] - Anti-PP2C gamma/PPM1G rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: 292T Whole Cell Lysate. Lane C: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 59 kDa. Observed band size: 80 kDa (We are unsure as to the identity of these extra bands).")
![Immunoprecipitation: PP2C gamma/PPM1G Antibody [NBP2-99758] - PP2C gamma/PPM1G was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. Lane B: 0.5 mg 293T Whole Cell Lysate 2 ul anti-PP2C gamma/PPM1G rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-PP2C gamma/PPM1G rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 59 kDa. Observed band size: 72 kDa](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Immunoprecipitation-NBP2-99758-img0004.jpg)