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![Western Blot: Max Antibody (103) [NBP2-90153] - Lane A: Jurkat Whole Cell LysateLane B: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:18 kDaObserved band size:20 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/Max-Antibody-103-Western-Blot-NBP2-90153-img0004.jpg "Western Blot: Max Antibody (103) [NBP2-90153] - Lane A: Jurkat Whole Cell LysateLane B: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:18 kDaObserved band size:20 kDa(We are unsure as to the identity of these extra bands.)")
![Immunocytochemistry/Immunofluorescence: Max Antibody (103) [NBP2-90153] - Staining of Human MAX in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human MAX monoclonal antibody (1:300) at 37? 1 hour. Then cells were stained with the Alexa Fluor® 594-conjugated goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to nucleus.](http://images.novusbio.com/fullsize/Max-Antibody-103-Immunocytochemistry-Immunofluorescence-NBP2-90153-img0001.jpg)
![Flow Cytometry: Max Antibody (103) [NBP2-90153] - Analysis of Human MAX expression on Raji cells stained with purified anti-Human MAX, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/Max-Antibody-103-Flow-Cytometry-NBP2-90153-img0002.jpg)
![Immunoprecipitation: Max Antibody (103) [NBP2-90153] - Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg 293T Whole Cell Lysate0.5 uL anti-MAX rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-MAX rabbit monoclonal antibody,at 1:1000 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 23 kDaObserved band size: 23 kDa](http://images.novusbio.com/fullsize/Max-Antibody-103-Immunoprecipitation-NBP2-90153-img0003.jpg)
![Western Blot: Max Antibody [NB100-793] - Staining of Jurkat lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Max-Antibody-Western-Blot-NB100-793-img0002.jpg "Western Blot: Max Antibody [NB100-793] - Staining of Jurkat lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Max Antibody [NB100-793] - Staining of Prostate. Antibody at 10 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Max-Antibody-Immunohistochemistry-Paraffin-NB100-793-img0003.jpg)
![Immunofluorescence: Max Antibody [NB100-793] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/Max-Antibody-Immunofluorescence-NB100-793-img0004.jpg)
![Immunofluorescence: Max Antibody [NB100-793] - Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/Max-Antibody-Immunofluorescence-NB100-793-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Max Antibody (4E10-1A9) [H00004149-M01] - Analysis of monoclonal antibody to MAX on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Max-Antibody-4E10-1A9-Immunocytochemistry-Immunofluorescence-H00004149-M01-img0006.jpg "Immunocytochemistry/Immunofluorescence: Max Antibody (4E10-1A9) [H00004149-M01] - Analysis of monoclonal antibody to MAX on HeLa cell . Antibody concentration 10 ug/ml.")
![Proximity Ligation Assay: Max Antibody (4E10-1A9) [H00004149-M01] - Analysis of protein-protein interactions between MAPK14 and MAX. HeLa cells were stained with anti-MAPK14 rabbit purified polyclonal 1:1200 and anti-MAX mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Max-Antibody-4E10-1A9-Proximity-Ligation-Assay-H00004149-M01-img0004.jpg)
![ELISA: Max Antibody (4E10-1A9) [H00004149-M01] - Max Antibody (4E10-1A9) [H00004149-M01] - Detection limit for recombinant GST tagged MAX is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Max-Antibody-4E10-1A9-ELISA-H00004149-M01-img0008.jpg)
![Western Blot: Max Antibody [NBP1-49963] - Detection of Human MAX by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MAX antibody used for WB at 0.1 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. For blotting immunoprecipitated MAX was used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).](http://images.novusbio.com/fullsize/Max-Antibody-Western-Blot-NBP1-49963-img0005.jpg "Western Blot: Max Antibody [NBP1-49963] - Detection of Human MAX by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MAX antibody used for WB at 0.1 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. For blotting immunoprecipitated MAX was used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).")
![Immunohistochemistry-Paraffin: Max Antibody [NBP1-49963] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-MAX used at a dilution of 1:1,000 (1ug/ml). Detection: Vector ImmPACT NovaRED](http://images.novusbio.com/fullsize/Max-Antibody-Immunohistochemistry-NBP1-49963-img0009.jpg)
![Western Blot: Max Antibody [NBP2-17233] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with MAX antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Max-Antibody-Western-Blot-NBP2-17233-img0006.jpg "Western Blot: Max Antibody [NBP2-17233] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with MAX antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Western Blot: Max Antibody [NBP2-17233] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with MAX antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Max-Antibody-Western-Blot-NBP2-17233-img0003.jpg)
![Western Blot: Max Antibody [NBP2-17233] - Wild-type (WT) and MAX knockout (KO) 293T cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with MAX antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Max-Antibody-Western-Blot-NBP2-17233-img0004.jpg)
![Immunoprecipitation: Max Antibody [NBP2-17233] - IP Sample: 293T whole cell lysate/extract A. Control with 2 ug of preimmune rabbit IgG B. Immunoprecipitation of MAX protein by 2 ug of MAX antibody 15% SDS-PAGE The immunoprecipitated MAX protein was detected by MAX antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Max-Antibody-Immunoprecipitation-NBP2-17233-img0002.jpg)
![Western Blot: Max Antibody [NBP3-00047] - Anti-Max rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 18 kDa. Observed band size: 23 kDa](http://images.novusbio.com/fullsize/Max-Antibody-Western-Blot-NBP3-00047-img0004.jpg "Western Blot: Max Antibody [NBP3-00047] - Anti-Max rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 18 kDa. Observed band size: 23 kDa")
![Immunoprecipitation: Max Antibody [NBP3-00047] - Max was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. Lane B: 0.5 mg Hela Whole Cell Lysate. Lane C: 0.5 mg 293T Whole Cell Lysate. Lane D:0.5 mg A549 Whole Cell Lysate. 2 ul anti-Max rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Max rabbit polyclonal antibody, at 1:330 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 23 kDa. Observed band size: 23 kDa](http://images.novusbio.com/fullsize/Max-Antibody-Immunoprecipitation-NBP3-00047-img0003.jpg)
![Immunohistochemistry-Paraffin: Max Antibody [NBP1-88624] - Staining of human appendix shows nuclear positivity in lymphoid tissue.](http://images.novusbio.com/fullsize/Max-Antibody-Immunohistochemistry-Paraffin-NBP1-88624-img0005.jpg "Immunohistochemistry-Paraffin: Max Antibody [NBP1-88624] - Staining of human appendix shows nuclear positivity in lymphoid tissue.")
![Western Blot: MAX Overexpression Lysate (Adult Normal) [NBP2-05496] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MAX.](http://images.novusbio.com/fullsize/MAX-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-05496-img0001.jpg "Western Blot: MAX Overexpression Lysate (Adult Normal) [NBP2-05496] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MAX.")
![Western Blot: MAX Overexpression Lysate (Adult Normal) [NBL1-12919] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MAX.](http://images.novusbio.com/fullsize/MAX-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12919-img0002.jpg "Western Blot: MAX Overexpression Lysate (Adult Normal) [NBL1-12919] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MAX.")
![Western Blot: MAX Overexpression Lysate (Adult Normal) [NBL1-12920] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MAX.](http://images.novusbio.com/fullsize/MAX-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12920-img0002.jpg "Western Blot: MAX Overexpression Lysate (Adult Normal) [NBL1-12920] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MAX.")
![Western Blot: MAX Overexpression Lysate (Adult Normal) [NBL1-12921] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MAX.](http://images.novusbio.com/fullsize/MAX-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12921-img0002.jpg "Western Blot: MAX Overexpression Lysate (Adult Normal) [NBL1-12921] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MAX.")