| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2736SS | |||
| Azide and BSA Free | NBP2-80845 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1244-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1244-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05384-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-21092 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-1312-0.025ml | |||
![Western Blot: MDM2/HDM2 Antibody (SMP14) [NB100-2736] - Daudi whole cell protein was separated by SDS-PAGE on a 7.5% gel and transferred to PVDF membrane. The membrane was probed with anti-MDM2 antibody at 2 ug/ml and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-SMP14-Western-Blot-NB100-2736-img0001.jpg "Western Blot: MDM2/HDM2 Antibody (SMP14) [NB100-2736] - Daudi whole cell protein was separated by SDS-PAGE on a 7.5% gel and transferred to PVDF membrane. The membrane was probed with anti-MDM2 antibody at 2 ug/ml and detected with an anti-mouse HRP secondary antibody using chemiluminescence.")
![Immunohistochemistry-Paraffin: MDM2/HDM2 Antibody (SMP14) [NB100-2736] - Analysis of tissue section of human breast cancer xenograft using MDM2/HDM2 antibody (clone SMP14) at 1:100 dilution. Several of the cancer cells developed a strong nuclear with weak cytoplasmic immunostaining of MDM2.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-SMP14-Immunohistochemistry-Paraffin-NB100-2736-img0003.jpg)
![Immunohistochemistry-Paraffin: MDM2/HDM2 Antibody (SMP14) [NB100-2736] - Analysis of tissue section of human breast cancer xenograft using MDM2/HDM2 antibody (clone SMP14) at 1:100 dilution. Several of the cancer cells developed a strong nuclear with weak cytoplasmic immunostaining of MDM2.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-SMP14-Immunohistochemistry-Paraffin-NB100-2736-img0002.jpg)
![Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Lack of HIF-P4H-1 increases the amount of p53. Western blot analysis of p53 and MDM2/HDM2 in wt MEFs treated with 10 uM nutlin-3a for 24 h with or without overexpression of human HIF-P4H-1 (OE). Anti-beta-actin (NB600-501 1:20 000) Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41598-017-17376-0) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-Western-Blot-NBP2-17247-img0027.jpg "Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Lack of HIF-P4H-1 increases the amount of p53. Western blot analysis of p53 and MDM2/HDM2 in wt MEFs treated with 10 uM nutlin-3a for 24 h with or without overexpression of human HIF-P4H-1 (OE). Anti-beta-actin (NB600-501 1:20 000) Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41598-017-17376-0) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: MDM2/HDM2 Antibody [NBP2-17247] - HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: MDM2 stained by MDM2 antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI. Scale bar= 10um.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-17247-img0029.jpg)
![Immunohistochemistry-Paraffin: MDM2/HDM2 Antibody [NBP2-17247] - Human lung cancer. MDM2 stained by MDM2 antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-Immunohistochemistry-Paraffin-NBP2-17247-img0028.jpg)
![Chromatin Immunoprecipitation: MDM2/HDM2 Antibody [NBP2-17247] - Cross-linked ChIP was performed with PC-3 chromatin extract and 5 ug of either control rabbit IgG or anti-MDM2 antibody. The precipitated DNA was detected by PCR with primer set targeting to PSA promoter.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-Chromatin-Immunoprecipitation-NBP2-17247-img0008.jpg)
![Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug GL261 whole cell lysate/extract C. 30 ug C8D30 whole cell lysate/extract D. 30 ug NIH-3T3 whole cell lysate/extract E. 30 ug BCL-1 whole cell lysate/extract F. 30 ug Raw264.7 whole cell lysate/extract G. 30 ug C2C12 whole cell ysate/extract 7.5 % SDS-PAGE MDM2 antibody dilution: 1:1000](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-Western-Blot-NBP2-17247-img0022.jpg)
![Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDM2 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-Western-Blot-NBP2-17247-img0023.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDM2 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Multiple bands were observed 60 ~ 97 kDa. It is possibly due to alternative splicing.Reference:PMID: 9840926](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-Western-Blot-NBP2-17247-img0024.jpg)
![Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDM2 antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-Western-Blot-NBP2-17247-img0026.jpg)
![Immunohistochemistry-Paraffin: MDM2/HDM2 Antibody [NBP2-17247] - Human lung cancer, using MDM2 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-Immunohistochemistry-Paraffin-NBP2-17247-img0003.jpg)
![Immunoprecipitation: MDM2/HDM2 Antibody [NBP2-17247] - IP samples: Jurkat whole cell extract A. 30 ug Jurkat cell whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of MDM2 protein by 4 ug MDM2 antibody 7.5 % SDS-PAGE The immunoprecipitated MDM2 protein was detected by MDM2 antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-Immunoprecipitation-NBP2-17247-img0021.jpg)
![ELISA MDM2/HDM2 [Biotin]](http://images.novusbio.com/fullsize2/dyc1244-2_human-total-mdm2-hdm2-duoset-ic-elisa-2-plate-elisa-1092020101328.jpg "ELISA MDM2/HDM2 [Biotin]")
![Western Blot: MDM2/HDM2 Antibody (1A7) [H00004193-M01] - Analysis of MDM2 expression in transfected 293T cell line by MDM2 monoclonal antibody (M01), clone 1A7.Lane 1: MDM2 transfected lysate(55.2 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-1A7-Western-Blot-H00004193-M01-img0018.jpg "Western Blot: MDM2/HDM2 Antibody (1A7) [H00004193-M01] - Analysis of MDM2 expression in transfected 293T cell line by MDM2 monoclonal antibody (M01), clone 1A7.Lane 1: MDM2 transfected lysate(55.2 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: MDM2/HDM2 Antibody (1A7) [H00004193-M01] - Analysis of monoclonal antibody to MDM2 on formalin-fixed paraffin-embedded human cerebral cortex. Antibody concentration 1.5 ug/ml.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-1A7-Immunohistochemistry-Paraffin-H00004193-M01-img0015.jpg)
![Western Blot: MDM2/HDM2 Antibody (1A7) [H00004193-M01] - Analysis of MDM2 over-expressed 293 cell line, cotransfected with MDM2 Validated Chimera RNAi ( Cat # H00004193-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MDM2 monoclonal antibody (M01), clone 1A7 (Cat # H00004193-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-1A7-Western-Blot-H00004193-M01-img0016.jpg)
![Proximity Ligation Assay: MDM2/HDM2 Antibody (1A7) [H00004193-M01] - Analysis of protein-protein interactions between TP53 and MDM2. HeLa cells were stained with anti-TP53 rabbit purified polyclonal 1:1200 and anti-MDM2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-1A7-Proximity-Ligation-Assay-H00004193-M01-img0013.jpg)
![ELISA: MDM2/HDM2 Antibody (1A7) [H00004193-M01] - Detection limit for recombinant GST tagged MDM2 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-1A7-ELISA-H00004193-M01-img0019.jpg)
![Western Blot: MDM2/HDM2 [p Ser185] Antibody [NB100-1683] - Shown in a 293T whole cell lysate. Cells were serum-starved for 24 hours prior to harvest. Approximately 20 ug of lysate was loaded per lane for SDS-PAGE. Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100 ng/ml) for 20 min prior to harvest. Follow reaction of antibody with a 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization.](http://images.novusbio.com/fullsize/MDM2-HDM2 [p Ser185] Antibody-Western Blot-NB100-1683-img0003.jpg "Western Blot: MDM2/HDM2 [p Ser185] Antibody [NB100-1683] - Shown in a 293T whole cell lysate. Cells were serum-starved for 24 hours prior to harvest. Approximately 20 ug of lysate was loaded per lane for SDS-PAGE. Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100 ng/ml) for 20 min prior to harvest. Follow reaction of antibody with a 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization.")
![Western Blot: MDM2/HDM2 [p Ser166] Antibody [NBP3-05384] - Western blot analysis of extracts of 293 cells, using MDM2/HDM2 antibody (NBP3-05384). 293 cells were treated by Hydroxyurea (4mM) for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.](http://images.novusbio.com/fullsize/MDM2-HDM2-[p-Ser166]-Antibody-Western-Blot-NBP3-05384-img0002.jpg "Western Blot: MDM2/HDM2 [p Ser166] Antibody [NBP3-05384] - Western blot analysis of extracts of 293 cells, using MDM2/HDM2 antibody (NBP3-05384). 293 cells were treated by Hydroxyurea (4mM) for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.")
![Western Blot: MDM2/HDM2 Antibody (HDM2-323) [NB600-1312] - Whole extracts of HEK-293 cells overexpressing human (lane 1) or mouse (lane 2) MDM2 were separated on SDS-PAGE and probed with 2 ug/mL Monoclonal Anti-MDM2 Clone: HDM2-323. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-HDM2-323-Western-Blot-NB600-1312-img0003.jpg "Western Blot: MDM2/HDM2 Antibody (HDM2-323) [NB600-1312] - Whole extracts of HEK-293 cells overexpressing human (lane 1) or mouse (lane 2) MDM2 were separated on SDS-PAGE and probed with 2 ug/mL Monoclonal Anti-MDM2 Clone: HDM2-323. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate.")
![Western Blot: MDM2 Overexpression Lysate (Adult Normal) [NBL1-12970] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MDM2.](http://images.novusbio.com/fullsize/MDM2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12970-img0002.jpg "Western Blot: MDM2 Overexpression Lysate (Adult Normal) [NBL1-12970] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MDM2.")