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![Immunohistochemistry: Importin alpha 2/KPNA2 Antibody [NB100-79807] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- RCH1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-Immunohistochemistry-NB100-79807-img0011.jpg "Immunohistochemistry: Importin alpha 2/KPNA2 Antibody [NB100-79807] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- RCH1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Importin alpha 2/KPNA2 Antibody [NB100-79807] - Detection of human RCH1Importin alpha 2/KPNA2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RCH1 antibody NB100-79807 (lot 2) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. RCH1 was also immunoprecipitated by a previous lot of NB100-79807 (lot 1) and by rabbit anti-RCH1 antibody NB100-79808, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-Immunoprecipitation-NB100-79807-img0012.jpg)
![Western Blot: Importin alpha 2/KPNA2 Antibody [NB100-79808] - Whole cell lysate (10 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RCH1/KPNA2 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-Western-Blot-NB100-79808-img0014.jpg "Western Blot: Importin alpha 2/KPNA2 Antibody [NB100-79808] - Whole cell lysate (10 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RCH1/KPNA2 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
![Immunohistochemistry-Paraffin: Importin alpha 2/KPNA2 Antibody [NB100-79808] - FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-RCH1/KPNA2 used at 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-Immunohistochemistry-Paraffin-NB100-79808-img0012.jpg)
![Western Blot: Importin alpha 2/KPNA2 Antibody [NB100-79808] - Whole cell lysate (10 ug) from HEK293T, HeLa, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RCH1/KPNA2 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-Western-Blot-NB100-79808-img0013.jpg)
![Immunohistochemistry-Paraffin: Importin alpha 2/KPNA2 Antibody [NB100-79808] - FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbitanti-RCH1/KPNA2 used at 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-Immunohistochemistry-Paraffin-NB100-79808-img0011.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Importin alpha 2/KPNA2 Antibody [NB100-79808] - Detection of human RCH1/KPNA2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RCH1/KPNA2 antibody NB100-79808 (lot NB100-79808-2) used for IP at 6 ug per reaction. RCH1/KPNA2 was also immunoprecipitated by a previous lot of this antibody (lot NB100-79808-1) and rabbit anti-RCH1/KPNA2 antibody NB100-79807. For blotting immunoprecipitated RCH1/KPNA2, NB100-79808 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-Immunoprecipitation-NB100-79808-img0010.jpg)
![Western Blot: Importin alpha 2/KPNA2 Antibody [NBP2-38482] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-Western-Blot-NBP2-38482-img0003.jpg "Western Blot: Importin alpha 2/KPNA2 Antibody [NBP2-38482] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG")
![Immunocytochemistry/Immunofluorescence: Importin alpha 2/KPNA2 Antibody [NBP2-38482] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38482-img0004.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Importin alpha 2/KPNA2 Antibody [NBP2-38482] - Staining in human testis and pancreas tissues using anti-KPNA2 antibody. Corresponding KPNA2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-Immunohistochemistry-Paraffin-NBP2-38482-img0007.jpg)
![Immunohistochemistry: Importin alpha 2/KPNA2 Antibody [NBP2-38482] - Staining of human testis shows strong nuclear and cytoplasmic positivity in cells in seminiferus ducts.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-Immunohistochemistry-NBP2-38482-img0002.jpg)
![Immunohistochemistry-Paraffin: Importin alpha 2/KPNA2 Antibody [NBP2-38482] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-Immunohistochemistry-Paraffin-NBP2-38482-img0005.jpg)
![Immunohistochemistry-Paraffin: Importin alpha 2/KPNA2 Antibody [NBP2-38482] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-Immunohistochemistry-Paraffin-NBP2-38482-img0006.jpg)
![Western Blot: Importin alpha 2/KPNA2 Antibody [NBP1-76298] - Western blot analysis of KPNA2 expression in Daudi cell lysate with KPNA2 antibody at (A) 1 and (B) 2 ug/ml.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-Western-Blot-NBP1-76298-img0005.jpg "Western Blot: Importin alpha 2/KPNA2 Antibody [NBP1-76298] - Western blot analysis of KPNA2 expression in Daudi cell lysate with KPNA2 antibody at (A) 1 and (B) 2 ug/ml.")
![Immunocytochemistry/Immunofluorescence: Importin alpha 2/KPNA2 Antibody [NBP1-76298] - Immunofluorescence of KPNA2 in human heart tissue with KPNA2 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76298-img0004.jpg)
![Immunohistochemistry: Importin alpha 2/KPNA2 Antibody [NBP1-76298] - Immunohistochemistry of KPNA2 in human heart tissue with KPNA2 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-Immunohistochemistry-NBP1-76298-img0003.jpg)
![Western Blot: Importin alpha 2/KPNA2 Antibody [NBP1-76298] - Rat heart tissue lysate with KPNA2 antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-Western-Blot-NBP1-76298-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Importin alpha 2/KPNA2 Antibody [NBP1-31098] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: karyopherin alpha 2 protein stained by karyopherin alpha 2 antibody [C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31098-img0010.jpg "Immunocytochemistry/Immunofluorescence: Importin alpha 2/KPNA2 Antibody [NBP1-31098] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: karyopherin alpha 2 protein stained by karyopherin alpha 2 antibody [C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.")
![Immunohistochemistry-Paraffin: Importin alpha 2/KPNA2 Antibody [NBP1-31098] - Human lung adenocarcinoma. karyopherin alpha 2 antibody [C3], C-term diluted at 1:250. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-Immunohistochemistry-Paraffin-NBP1-31098-img0008.jpg)
![Importin alpha 2/KPNA2 Antibody [NBP1-31098] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with KPNA2 antibody [C3], C-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-NBP1-31098-img0011.jpg)
![Western Blot: Importin alpha 2/KPNA2 Antibody (9T3V8) [NBP3-16530] - Western blot analysis of extracts of various cell lines, using Importin alpha 2/KPNA2 Rabbit mAb (NBP3-16530) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-9T3V8-Western-Blot-NBP3-16530-img0001.jpg "Western Blot: Importin alpha 2/KPNA2 Antibody (9T3V8) [NBP3-16530] - Western blot analysis of extracts of various cell lines, using Importin alpha 2/KPNA2 Rabbit mAb (NBP3-16530) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Western Blot: Importin alpha 2/KPNA2 Antibody [NBP1-58067] - Transfected 293T cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-Western-Blot-NBP1-58067-img0003.jpg "Western Blot: Importin alpha 2/KPNA2 Antibody [NBP1-58067] - Transfected 293T cell lysate, concentration 0.2-1 ug/ml.")