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![Immunocytochemistry/Immunofluorescence: Goat anti-Human IgM Heavy Chain Secondary Antibody [NBP2-60670] - Immunocytochemistry/Immunofluorescence: Goat anti-Human IgM Secondary Antibody [DyLight 488] [NBP1-72860]](http://images.novusbio.com/fullsize/Goat-anti-Human-IgM-Heavy-Chain-Secondary-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-60670-img0002.jpg "Immunocytochemistry/Immunofluorescence: Goat anti-Human IgM Heavy Chain Secondary Antibody [NBP2-60670] - Immunocytochemistry/Immunofluorescence: Goat anti-Human IgM Secondary Antibody [DyLight 488] [NBP1-72860]")
![Fluorophore-linked immunosorbent assay: Goat anti-Human IgM Heavy Chain Secondary Antibody [NBP2-60670] - Image using the DyLight 405 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Human-IgM-Heavy-Chain-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NBP2-60670-img0003.jpg)
![Fluorophore-linked immunosorbent assay: Goat anti-Human IgM Heavy Chain Secondary Antibody [NBP2-60670] - Image using the DyLight 488 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Human-IgM-Heavy-Chain-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NBP2-60670-img0004.jpg)
![ELISA: Goat anti-Human IgM Heavy Chain Secondary Antibody [NBP2-60670] - Image using the DyLight 680 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Human-IgM-Heavy-Chain-Secondary-Antibody-ELISA-NBP2-60670-img0005.jpg)
![Fluorophore-linked immunosorbent assay: Goat anti-Human IgM Heavy Chain Secondary Antibody [NBP2-60670] - Image using the DyLight 800 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Human-IgM-Heavy-Chain-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NBP2-60670-img0008.jpg)
![ELISA: Goat anti-Human IgM Heavy Chain Secondary Antibody [NBP2-60670] - ELISA results of purified Goat anti-Human IgM Heavy Chain Secondary antibody tested against purified human IgM [RED line], human IgG F(ab')2 {PURPLE line], human IgG Fab [BLUE line], human IgG F(c) [GREEN line]. Each well was coated in duplicate with 1.0 ug of IgM, IgG F(ab')2, IgG Fab, IgG F(c). The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer, Donkey-anti-Goat IgG HRP conjugate 1:12, 000, and TMB substrate.](http://images.novusbio.com/fullsize/Goat-anti-Human-IgM-Heavy-Chain-Secondary-Antibody-ELISA-NBP2-60670-img0009.jpg)
![Dot Blot: Rabbit anti-Mouse IgM Heavy Chain Secondary Antibody [NBP1-72789] - Mouse IgM. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng.](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgM-Heavy-Chain-Secondary-Antibody-Dot-Blot-NBP1-72789-img0001.jpg "Dot Blot: Rabbit anti-Mouse IgM Heavy Chain Secondary Antibody [NBP1-72789] - Mouse IgM. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng.")
![ELISA: Rabbit anti-Mouse IgM Heavy Chain Secondary Antibody [NBP1-72789] - ELISA Results of Rabbit anti-Mouse IgM Heavy Chain Secondary antibody tested against purified Mouse IgM. Each well was coated in duplicate with 10 ug of Mouse IgM. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking buffer, Goat Anti-Rabbit IgG mx10 HRP conjugated, and TMB substrate.](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgM-Heavy-Chain-Secondary-Antibody-ELISA-NBP1-72789-img0004.jpg)