| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide Free | NBP2-33224 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide Free | NBP2-33220 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2684-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF4365-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76799-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-216SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-215SS | |||
![In vivo assay: IKK gamma Inhibitor Peptide Set [NBP2-26504] - NBD peptide blocks constitutive NF-kB as shown by EMSA. U266 cells were treated with 100 uM of control or NBD peptide for different time periods. Nuclear extracts were isolated and checked for NF-kB-DNA binding activity.](http://images.novusbio.com/fullsize/IKK-gamma-Inhibitor-Peptide-Set-In-vivo-assay-NBP2-26504-img0010.jpg "In vivo assay: IKK gamma Inhibitor Peptide Set [NBP2-26504] - NBD peptide blocks constitutive NF-kB as shown by EMSA. U266 cells were treated with 100 uM of control or NBD peptide for different time periods. Nuclear extracts were isolated and checked for NF-kB-DNA binding activity.")
![In vivo assay: IKK gamma Inhibitor Peptide Set [NBP2-26504] - NBD peptide blocks constitutive NF-kB activation in human multiple myeloma cells. U266 cells were treated with 100 uM of control (A & B) or NBD peptide (C & D) for 12 hr, cytospun, plated on glass slides, air dried for 1 hr at room temperature and fixed with cold acetone. Slides were blocked with 5% normal goat serum for 1 hr and then incubated with rabbit polyclonal anti-human p65 antibody (A & C) followed by Ig-Alexa 594 second step. In control peptide treated cells, p65 translocates to nucleus (A), whereas NBD peptide prevents translocation of p65 into the nucleus (C). B & D: Nuclear staining with DNA binding dye.](http://images.novusbio.com/fullsize/IKK-gamma-Inhibitor-Peptide-Set-In-vivo-assay-NBP2-26504-img0012.jpg)
![IKK gamma Inhibitor Peptide Set [NBP2-26504] - The IKK complex consists of IKKalpha + IKKbeta+ NEMO. NEMO (IKKgamma/NF-kB Essential MOdulator) is a scaffold protein required for the kinase activity of IKKalpha + IKKbeta. NBD (NEMO Binding Domain) inhibits formation of the IKK complex (IKKalpha + IKKbeta+ NEMO) by binding to NEMO and blocking IKK complex formation. In this regard, NBD is considered to be an 'IKKalpha and IKKBeta decoy' Blocking IKK complex formation suppresses downstream events in the NF-kB signaling pathway that rely on the IKK complex including phosphorylation of IkBalpha, and subsequence IkBalpha ubiquination, degradation and release of NF-kB. Hence, NF-kB is retained in the cytoplasm in its inactive state.](http://images.novusbio.com/fullsize/IKK-gamma-Inhibitor-Peptide-Set-NBP2-26504-img0014.jpg)
![Functional (Inhibition): IKK gamma Inhibitor Peptide Set [NBP2-26504] - TLR5/NF-kB/SEAPorter HEK 293 (NBP2-26277) cells were plated in 96-well plates at 5 x 10^4 cells/well for 16 h. Cells were preincubated with different concentrations (0, 1, 5, 10, 25 and 50 uM) of Inhibitory Peptide (NBP2-26504) and Control Peptide (NBP2-29334) for 1 h. Cells were then stimulated with 1 ng/ml Flagellin (NBP2-25289) for 24 h. Secreted alkaline phosphatase (SEAP) was analyzed using SEAPorter Assay Kit (NBP2-25285). *p < 0.05 versus control peptide at the corresponding concentrations (Mann-Whitney U test).](http://images.novusbio.com/fullsize/IKK-gamma-Inhibitor-Peptide-Set-Functional-Inhibition-NBP2-26504-img0016.jpg)
![Western Blot: IKK gamma Antibody (72C627) [NB100-56542] - Analysis using IKK gamma (NEMO) antibody. Lysate from 1) human Jurkat and 2) mouse NIH 3T3 cells probed with IKK gamma antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/IKK-gamma-Antibody-72C627-Western-Blot-NB100-56542-img0005.jpg "Western Blot: IKK gamma Antibody (72C627) [NB100-56542] - Analysis using IKK gamma (NEMO) antibody. Lysate from 1) human Jurkat and 2) mouse NIH 3T3 cells probed with IKK gamma antibody at 2 ug/ml.")
![Simple Western: IKK gamma Antibody (72C627) [NB100-56542] - Simple Western lane view shows a specific band for IKK gamma in 0.05 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/IKK-gamma-Antibody-72C627-Simple-Western-NB100-56542-img0004.jpg)
![Western Blot: IKK gamma Antibody (46B844) [NB100-56532] - Analysis using NEMO (IKKgamma) antibody. Lysate from human Jurkat cells probed with NEMO antibody at 2 ug/ml. The goat anti-mouse IgG1 HRP secondary and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/IKK-gamma-Antibody-46B844-Western-Blot-NB100-56532-img0007.jpg "Western Blot: IKK gamma Antibody (46B844) [NB100-56532] - Analysis using NEMO (IKKgamma) antibody. Lysate from human Jurkat cells probed with NEMO antibody at 2 ug/ml. The goat anti-mouse IgG1 HRP secondary and PicoTect ECL substrate solution were used for this test.")
![Flow Cytometry: IKK gamma Antibody (46B844) [NB100-56532] - Human Jurkat cells were probed using 0.1 ug of NEMO antibody (red) and 0.1 ug of isotype control (green), with shaded histogram representing cells alone and an anti-mouse IgG-FITC secondary antibody.](http://images.novusbio.com/fullsize/IKK-gamma-Antibody-46B844-Flow-Cytometry-NB100-56532-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: IKK gamma Antibody (HL1577) - Azide and BSA Free [NBP3-25521] - IKK gamma antibody [HL1577] detects IKK gamma protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IKK gamma stained by IKK gamma antibody [HL1577] (NBP3-25521) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp3-25521_rabbit-ikk-gamma-mab-hl1577-azide-and-bsa-free-212024939145.jpg "Immunocytochemistry/Immunofluorescence: IKK gamma Antibody (HL1577) - Azide and BSA Free [NBP3-25521] - IKK gamma antibody [HL1577] detects IKK gamma protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IKK gamma stained by IKK gamma antibody [HL1577] (NBP3-25521) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.")
![Immunohistochemistry-Paraffin: IKK gamma Antibody (HL1577) - Azide and BSA Free [NBP3-25521] - IKK gamma antibody [HL1577] detects IKK gamma protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. IKK gamma stained by IKK gamma antibody [HL1577] (NBP3-25521) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25521_rabbit-ikk-gamma-mab-hl1577-azide-and-bsa-free-212024943285.jpg)
![Western Blot: IKK gamma Antibody (HL1577) - Azide and BSA Free [NBP3-25521] - Whole cell extract (10 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with IKK gamma antibody [HL1577] (NBP3-25521) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25521_rabbit-ikk-gamma-mab-hl1577-azide-and-bsa-free-2120249432859.jpg)
![Western Blot: IKK gamma Antibody (HL1577) - Azide and BSA Free [NBP3-25521] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IKK gamma antibody [HL1577] (NBP3-25521) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25521_rabbit-ikk-gamma-mab-hl1577-azide-and-bsa-free-212024941426.jpg)
![Western Blot: IKK gamma Antibody (HL1577) - Azide and BSA Free [NBP3-25521] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with IKK gamma antibody [HL1577] (NBP3-25521) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25521_rabbit-ikk-gamma-mab-hl1577-azide-and-bsa-free-2120249393559.jpg)
![Western Blot: IKK gamma Antibody [NBP1-84681] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/IKK-gamma-Antibody-Western-Blot-NBP1-84681-img0008.jpg "Western Blot: IKK gamma Antibody [NBP1-84681] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunocytochemistry/Immunofluorescence: IKK gamma Antibody [NBP1-84681] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.](http://images.novusbio.com/fullsize/IKK-gamma-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84681-img0009.jpg)
![Immunohistochemistry-Paraffin: IKK gamma Antibody [NBP1-84681] - Staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/IKK-gamma-Antibody-Immunohistochemistry-Paraffin-NBP1-84681-img0002.jpg)
![Western Blot: IKK gamma Antibody [NBP1-84681] - Analysis in human cell line HepG2.](http://images.novusbio.com/fullsize/IKK-gamma-Antibody-Western-Blot-NBP1-84681-img0007.jpg)
![Western Blot: IKK gamma Antibody [NBP1-76799] - Analysis of IKKg in HeLa whole cell lysate with anti-IKKg/NEMO using NBP1-76799 at 1 ug/ml.](http://images.novusbio.com/fullsize/IKK-gamma-Antibody-Western-Blot-NBP1-76799-img0003.jpg "Western Blot: IKK gamma Antibody [NBP1-76799] - Analysis of IKKg in HeLa whole cell lysate with anti-IKKg/NEMO using NBP1-76799 at 1 ug/ml.")
![Immunocytochemistry/Immunofluorescence: IKK gamma Antibody [NBP1-76799] - Hela cells with IKK gamma antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/IKK-gamma-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76799-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: IKK gamma Antibody [NBP1-76799] - HeLa cells with IKK gamma antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/IKK-gamma-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76799-img0002.jpg)
![Western Blot: IKK gamma Antibody (46B844) - Azide Free [NBP2-33220] - Analysis using NEMO (IKKgamma) antibody. Lysate from human Jurkat cells probed with NEMO antibody at 2 ug/ml. The goat anti-mouse IgG1 HRP secondary and PicoTect ECL substrate solution were used for this test. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/IKK-gamma-Antibody-46B844-Azide-Free-Western-Blot-NBP2-33220-img0001.jpg "Western Blot: IKK gamma Antibody (46B844) - Azide Free [NBP2-33220] - Analysis using NEMO (IKKgamma) antibody. Lysate from human Jurkat cells probed with NEMO antibody at 2 ug/ml. The goat anti-mouse IgG1 HRP secondary and PicoTect ECL substrate solution were used for this test. Image from the standard format of this antibody.")
![Flow Cytometry: IKK gamma Antibody (46B844) - Azide Free [NBP2-33220] - Human Jurkat cells were probed using 0.1 ug of NEMO antibody (red) and 0.1 ug of isotype control (green), with shaded histogram representing cells alone and an anti-mouse IgG-FITC secondary antibody. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/IKK-gamma-Antibody-46B844-Azide-Free-Flow-Cytometry-NBP2-33220-img0002.jpg)
![Western Blot: IKK gamma Antibody [NB600-216] - WB detection of IKK-gamma in Interleukin 1 stimulated HEK 293 cells at a dilution of 1:500.](http://images.novusbio.com/fullsize/IKK-gamma-Antibody-Western-Blot-NB600-216-img0002.jpg "Western Blot: IKK gamma Antibody [NB600-216] - WB detection of IKK-gamma in Interleukin 1 stimulated HEK 293 cells at a dilution of 1:500.")
![Western Blot: IKK-gamma Antibody [NB600-215] - WB detection of IKK-gamma in Interleuking 1 stimulated 293 cells at 1:500 dilution.](http://images.novusbio.com/fullsize/IKK-gamma-Antibody-Western-Blot-NB600-215-img0001.jpg "Western Blot: IKK-gamma Antibody [NB600-215] - WB detection of IKK-gamma in Interleuking 1 stimulated 293 cells at 1:500 dilution.")
![Western Blot: IKK gamma [p Ser376] Antibody [NBP3-13396] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IKK gamma (phospho Ser376) antibody (NBP3-13396) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/IKK-gamma-[p-Ser376]-Antibody-Western-Blot-NBP3-13396-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: IKK gamma [p Ser376] Antibody [NBP3-13396] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IKK gamma (phospho Ser376) antibody (NBP3-13396) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: IKK gamma [p Ser376] Antibody [NBP3-13396] - IKK gamma (phospho Ser376) antibody detects IKK gamma (phospho Ser376) protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. IKK gamma (phospho Ser376) stained by IKK gamma (phospho Ser376) antibody (NBP3-13396) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/IKK-gamma-[p-Ser376]-Antibody-Immunohistochemistry-Paraffin-NBP3-13396-img0002.jpg)