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23 Results for "novus"
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AF1614

Hepassocin/FGL1 Antibody [Unconjugated]

(1 Publication)
100 μg
AF1614
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Hepa 1‑6 mouse hepatoma cell line, and H4‑II‑E‑C3 rat hepatoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Hepassocin/FGL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1614) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Hepassocin/FGL1 homodimer at approximately 65 kDa (as indicated). Hepassocin/FGL1 is resistant to reducing conditions (1). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hu, Mu, Rt
Species Glossary
Reactivity: Hu, Mu, Rt
WB, Simple Western, Flow
Applications: WB, Simple Western, Flow
Goat Polyclonal
Host: Goat Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Biotin BAF1614
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free AF1614-SP
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AF1105

Hepassocin/FGL1 Antibody [Unconjugated]

(2 Publications)
100 μg
AF1105
Hepassocin/FGL1 was detected in perfusion fixed frozen sections of rat liver using 15 µg/mL Goat Anti-Rat Hepassocin/FGL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1105) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Rt
Species Glossary
Reactivity: Rt
WB, IHC
Applications: WB, IHC
Goat Polyclonal
Host: Goat Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Biotin BAF1105
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free AF1105-SP
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MAB16141

Hepassocin/FGL1 Antibody (1016010) [Unconjugated]

100 μg
MAB16141
Western blot shows lysates of human liver tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Hepassocin Monoclonal Antibody (Catalog # MAB16141) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Hepassocin at approximately 68 kDa for the dimer and 34 kDa for the monomer (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hu
Species Glossary
Reactivity: Hu
WB, IHC
Applications: WB, IHC
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB16141-SP
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MAB16142

Hepassocin/FGL1 Antibody (1016024) [Unconjugated]

100 μg
MAB16142
Hepassocin/FGL1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line (positive stain) and absent in K562 human leukemia cell line (negative stain) using Mouse Anti-Human Hepassocin/FGL1 Monoclonal Antibody (Catalog # MAB16142) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Hu
Species Glossary
Reactivity: Hu
CyTOF-ready, Flow, ICC/IF
Applications: CyTOF-ready, Flow, ICC/IF
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 IC16142U-100UG
Alexa Fluor 350 FAB16142U
Alexa Fluor 405 IC16142V-100UG
Alexa Fluor 405 FAB16142V
Alexa Fluor 488 IC16142G-100UG
Alexa Fluor 488 FAB16142G
Alexa Fluor 532 FAB16142X
Alexa Fluor 594 IC16142T-100UG
Alexa Fluor 594 FAB16142T
Alexa Fluor 647 FAB16142R
Alexa Fluor 647 IC16142R-100UG
Alexa Fluor 700 FAB16142N
Alexa Fluor 700 IC16142N-100UG
Alexa Fluor 750 FAB16142S
Alexa Fluor 750 IC16142S-100UG
Allophycocyanin FAB16142A
Biotin FAB16142B
DyLight 350 FAB16142D
DyLight 405 FAB16142E
DyLight 488 FAB16142K
DyLight 550 FAB16142L
DyLight 594 FAB16142M
DyLight 650 FAB16142W
DyLight 680 FAB16142Y
DyLight 755 FAB16142Z
FITC FAB16142F
HRP FAB16142H
Janelia Fluor 549 FAB16142I
Janelia Fluor 646 FAB16142J
PE FAB16142P
PerCP FAB16142C
mFluor Violet 450 SE FAB16142MFV450
mFluor Violet 500 SE FAB16142MFV500
mFluor Violet 610 SE FAB16142MFV610
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB16142-SP
Carrier Free IC16142V-100UG
Carrier Free IC16142U-100UG
Carrier Free IC16142T-100UG
Carrier Free IC16142S-100UG
Carrier Free IC16142R-100UG
Carrier Free IC16142N-100UG
Carrier Free IC16142G-100UG
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11176-HE

Recombinant Cynomolgus/Rhesus Hepassocin/FGL1 Fc Protein, CF

50 μg
11176-HE
Measured by its binding ability in a functional ELISA. When Recombinant Human LAG-3 Fc Chimera Protein (2319-L3) is immobilized at 2.50 μg/mL, 100 μL/well, Recombinant Cynomolgus Monkey/Rhesus Macaque Hepassocin/FGL1 Fc Chimera Protein (Catalog # 11176-HE) binds with an ED50 of 0.500-4.00 µg/mL.
Pm-Cm, RM
Species Glossary
Reactivity: Pm-Cm, RM
Bioactivity
Applications: Bioactivity
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MAB1614

Hepassocin/FGL1 Antibody (198401) [Unconjugated]

(1 Publication)
500 μg
Hu
Species Glossary
Reactivity: Hu
WB
Applications: WB
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB1614-SP
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10573-HE

Recombinant Human Hepassocin/FGL1 Fc Chimera Protein, CF

50 μg
10573-HE
When Recombinant Human LAG-3 Fc Chimera (2319-L3) is coated at 2.5 μg/mL (100 μL/well), the concentration of Recombinant Human Hepassocin/FGL1 Fc Chimera (Catalog # 10573-HE) that produces 50% of the optimal binding response is 0.2-2 μg/mL.
Hu
Species Glossary
Reactivity: Hu
Bioactivity
Applications: Bioactivity
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MAB112302

Hepassocin/FGL1 Antibody (1052936) [Unconjugated]

100 μg
MAB112302
Hepassocin/FGL1 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Hepassocin/FGL1 Monoclonal Antibody (Catalog # MAB112302) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Hu
Species Glossary
Reactivity: Hu
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB112302-SP
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10285-HE

Recombinant Human Hepassocin/FGL1 His-tag Protein, CF

50 μg
10285-HE
When Recombinant Human LAG-3 Fc Chimera (Catalog # 2319-L3) is coated at 1 μg/mL (100 μL/well), the concentration of Human Hepassocin/FGL1 His-tag (Catalog # 10285-HE) that produces 50% of the optimal binding response is 0.05‑0.4 μg/mL.
Hu
Species Glossary
Reactivity: Hu
Bioactivity
Applications: Bioactivity
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AVI10573

Recombinant Human Hepassocin/FGL1 Avi Fc Chimera Protein, CF

50 μg
AVI10573
Measured by its binding ability in a functional ELISA. When Recombinant Human LAG-3 Fc Chimera Protein (2319-L3) is immobilized at 2.50 µg/mL (100 µL/ well), Biotinylated Recombinant Human Hepassocin/FGL1 Avi-tag Fc Chimera (Catalog # AVI10573) binds with an ED50 of 0.250-2.50 μg/mL.
Hu
Species Glossary
Reactivity: Hu
Bioactivity
Applications: Bioactivity
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MAB1105

Hepassocin/FGL1 Antibody (160916) [Unconjugated]

500 μg
Rt
Species Glossary
Reactivity: Rt
WB
Applications: WB
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB1105-SP
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MAB11052

Hepassocin/FGL1 Antibody (160902) [Unconjugated]

100 μg
MAB11052
Recombinant Rat Hepassocin/FGL1 protein was serially diluted 2-fold and captured by Mouse Anti-Rat Hepassocin/FGL1 Monoclonal Antibody (Catalog # MAB11052) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Rat Hepassocin/FGL1Monoclonal Antibody (Catalog # MAB11051) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Rt
Species Glossary
Reactivity: Rt
ELISA
Applications: ELISA
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB11052-SP
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