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22 Results for "novus"
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AF956

Gremlin 1 Antibody [Unconjugated]

(1 Review)
(17 Publications)
100 μg
AF956
Gremlin was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human/Mouse Gremlin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF956) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Hu, Mu
Species Glossary
Reactivity: Hu, Mu
IHC, B/N
Applications: IHC, B/N
Goat Polyclonal
Host: Goat Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Biotin BAF956
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free AF956-SP
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5190-GR

Recombinant Human Gremlin Protein, CF

(17 Publications)
50 μg
5190-GR
Recombinant Human Gremlin (Catalog # 5190‑GR) inhibits BMP-4-induced alkaline phosphatase production in the MC3T3-E1 mouse preosteoblast cell line. The ED50 for this effect is 0.025‑0.15 µg/mL in the presence of 30 ng/mL of Recombinant Human BMP-4 (Catalog # 314‑BP).
Hu
Species Glossary
Reactivity: Hu
Bioactivity
Applications: Bioactivity
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NB100-56883

Gremlin 1 Antibody

(5 Publications)
0.1 mg
NB100-56883
Immunohistochemistry-Paraffin: Gremlin 1 Antibody [NB100-56883] - Detection of Gremlin in human colon cancer tissue using antibody at 5 ug/ml.
Hu, Mu, Rt
Species Glossary
Reactivity: Hu, Mu, Rt
IHC
Applications: IHC
Rabbit Polyclonal
Host: Rabbit Polyclonal
IMG-5136A
Alt Catalog# IMG-5136A
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
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DY956

Mouse Gremlin DuoSet ELISA, 15 Plate

(3 Publications)
1 Kit (for 15 Plates)
DY956
N/A Gremlin 1 [Biotin]
Mu
Species Glossary
Reactivity: Mu
ELISA
Applications: ELISA
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Qk015

Recombinant Human/Bovine Gremlin 1, Animal-Free Protein

50 μg
Qk015
Gremlin 1 activity is determined using inhibition of the BMP2 response (Qk007 #010, 52 ng/ml) from a BMP2-responsive firefly luciferase reporter in stably transfected HEK293T cells. Cells are treated (n=4) with a serial dilution of Gremlin 1 in BMP2 for 6 hours. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity.Gremlin-1 inhibits BMP-2 induced luciferase activity with an EC50 = 47.9 ng/ml (2.66 nM).
Hu, Po, Bv
Species Glossary
Reactivity: Hu, Po, Bv
Bioactivity
Applications: Bioactivity
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free Qk015-0500
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MAB956

Gremlin 1 Antibody (155606) [Unconjugated]

100 μg
Mu
Species Glossary
Reactivity: Mu
ELISA(Cap)
Applications: ELISA(Cap)
Rat Monoclonal
Host: Rat Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB956-SP
Carrier Free MAB956-500
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MAB51901

Gremlin 1 Antibody (955323) [Unconjugated]

100 μg
MAB51901
Recombinant Human Gremlin protein was serially diluted 2-fold and captured by Mouse Anti-Human Gremlin Monoclonal Antibody (Catalog # MAB51901) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Gremlin Monoclonal Antibody (Catalog # MAB5190) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Hu
Species Glossary
Reactivity: Hu
ELISA
Applications: ELISA
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB51901-SP
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DY5190-05

Human Gremlin DuoSet ELISA, 5 Plate

1 Kit (for 5 Plates)
DY5190-05
N/A Gremlin 1 [Biotin]
Hu
Species Glossary
Reactivity: Hu
ELISA
Applications: ELISA
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MAB5190

Gremlin 1 Antibody (955320) [Unconjugated]

100 μg
MAB5190
Recombinant Human Gremlin protein was serially diluted 2-fold and captured by Mouse Anti-Human Gremlin Monoclonal Antibody (Catalog # MAB51901) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Gremlin Monoclonal Antibody (Catalog # MAB5190) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Hu
Species Glossary
Reactivity: Hu
ELISA
Applications: ELISA
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB5190-SP
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NB100-56883PEP

Gremlin 1 Antibody Blocking Peptide

0.1 mg
AC
Applications: AC
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NBP2-75362

Human Gremlin 1 ELISA Kit (Chemiluminescence)

(1 Publication)
1 Kit
NBP2-75362
ELISA: Human Gremlin 1 ELISA Kit (Chemiluminescence) [NBP2-75362] - Samples were spiked with high concentrations of Human Gremlin 1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Hu
Species Glossary
Reactivity: Hu
ELISA
Applications: ELISA
31.25-2000 pg/mL
18.75 pg/mL
Serum, plasma and other biological fluids
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NBP2-75364

Rat Gremlin 1 ELISA Kit (Chemiluminescence)

(1 Publication)
1 Kit
NBP2-75364
ELISA: Rat Gremlin 1 ELISA Kit (Chemiluminescence) [NBP2-75364] - Samples were spiked with high concentrations of Rat Gremlin 1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Rt
Species Glossary
Reactivity: Rt
ELISA
Applications: ELISA
31.25-2000 pg/mL
18.75 pg/mL
Serum, plasma and other biological fluids
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