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![Immunohistochemistry-Paraffin: Granulin Antibody [NBP1-87324] - Staining in human placenta and skeletal muscle tissues . Corresponding GRN RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Granulin-Antibody-Immunohistochemistry-Paraffin-NBP1-87324-img0015.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Granulin Antibody [NBP1-87324] - Staining in human placenta and skeletal muscle tissues . Corresponding GRN RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Granulin Antibody [NBP1-87324] - Staining of human cell line A-431 shows localization to endoplasmic reticulum & lysosomes. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Granulin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87324-img0009.jpg)
![Immunohistochemistry-Paraffin: Granulin Antibody [NBP1-87324] - Staining of human cerebral cortex shows moderate granular cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/Granulin-Antibody-Immunohistochemistry-Paraffin-NBP1-87324-img0011.jpg)
![Immunohistochemistry-Paraffin: Granulin Antibody [NBP1-87324] - Staining of human kidney shows moderate granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Granulin-Antibody-Immunohistochemistry-Paraffin-NBP1-87324-img0012.jpg)
![Immunohistochemistry-Paraffin: Granulin Antibody [NBP1-87324] - Staining of human placenta shows moderate to strong granular cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Granulin-Antibody-Immunohistochemistry-Paraffin-NBP1-87324-img0013.jpg)
![Immunohistochemistry-Paraffin: Granulin Antibody [NBP1-87324] - Staining of human skeletal muscle shows very weak cytoplasmic positivity in striated muscle fibers.](http://images.novusbio.com/fullsize/Granulin-Antibody-Immunohistochemistry-Paraffin-NBP1-87324-img0014.jpg)
![Western Blot: Granulin Antibody [NBP1-32076] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Granulins antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Granulin-Antibody-Western-Blot-NBP1-32076-img0007.jpg "Western Blot: Granulin Antibody [NBP1-32076] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Granulins antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Granulin Antibody [NBP1-32076] - DIV9 rat E18 primary cortical neurons cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Granulins stained by Granulins antibody diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [1338] diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Granulin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32076-img0009.jpg)
![Immunohistochemistry-Paraffin: Granulin Antibody [NBP1-32076] - GRN protein at cytosol on HBL435 xenograft Sample: Paraffin-embedded HBL435 xenograft. Dilution: 1:500](http://images.novusbio.com/fullsize/Granulin-Antibody-Immunohistochemistry-Paraffin-NBP1-32076-img0004.jpg)
![Western Blot: Granulin Antibody [NBP1-32076] - Sample (30 ug of whole cell lysate) A: HepG2 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Granulin-Antibody-Western-Blot-NBP1-32076-img0002.jpg)
![Western Blot: Granulin Antibody [NBP1-32076] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Granulins antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Granulin-Antibody-Western-Blot-NBP1-32076-img0006.jpg)
![Western Blot: Granulin Antibody (CL5695) [NBP2-61425] - Analysis in human cell line PC-3.](http://images.novusbio.com/fullsize/Granulin-Antibody-CL5695-Western-Blot-NBP2-61425-img0007.jpg "Western Blot: Granulin Antibody (CL5695) [NBP2-61425] - Analysis in human cell line PC-3.")
![Immunohistochemistry-Paraffin: Granulin Antibody (CL5695) [NBP2-61425] - Staining of human skeletal muscle shows absence of positivity in striated muscle fibers as expected (negative control).](http://images.novusbio.com/fullsize/Granulin-Antibody-CL5695-Immunohistochemistry-Paraffin-NBP2-61425-img0004.jpg)
![Immunohistochemistry-Paraffin: Granulin Antibody (CL5695) [NBP2-61425] - Staining of human endometrium shows moderate granular cytoplasmic immunoreactivity in glandular cells.](http://images.novusbio.com/fullsize/Granulin-Antibody-CL5695-Immunohistochemistry-Paraffin-NBP2-61425-img0001.jpg)
![Immunohistochemistry-Paraffin: Granulin Antibody (CL5695) [NBP2-61425] - Staining of human liver shows cytoplasmic immunoreactivity in Kupffer cells.](http://images.novusbio.com/fullsize/Granulin-Antibody-CL5695-Immunohistochemistry-Paraffin-NBP2-61425-img0002.jpg)
![Immunohistochemistry-Paraffin: Granulin Antibody (CL5695) [NBP2-61425] - Staining of human prostate shows moderate granular cytoplasmic immunoreactivity in glandular cells.](http://images.novusbio.com/fullsize/Granulin-Antibody-CL5695-Immunohistochemistry-Paraffin-NBP2-61425-img0003.jpg)
![Western Blot: Granulin Antibody (OTI3H6) [NBP2-45915] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRN.](http://images.novusbio.com/fullsize/Granulin-Antibody-OTI3H6-Western-Blot-NBP2-45915-img0001.jpg "Western Blot: Granulin Antibody (OTI3H6) [NBP2-45915] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRN.")
![Immunohistochemistry-Paraffin: Granulin Antibody (OTI3H6) [NBP2-45915] - Staining of paraffin-embedded Human liver tissue using anti-GRN mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Granulin-Antibody-OTI3H6-Immunohistochemistry-Paraffin-NBP2-45915-img0005.jpg)
![Immunohistochemistry-Paraffin: Granulin Antibody (OTI3H6) [NBP2-45915] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-GRN mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Granulin-Antibody-OTI3H6-Immunohistochemistry-Paraffin-NBP2-45915-img0002.jpg)
![Immunohistochemistry-Paraffin: Granulin Antibody (OTI3H6) [NBP2-45915] - Staining of paraffin-embedded Human colon tissue using anti-GRN mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Granulin-Antibody-OTI3H6-Immunohistochemistry-Paraffin-NBP2-45915-img0003.jpg)
![Immunohistochemistry-Paraffin: Granulin Antibody (OTI3H6) [NBP2-45915] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-GRN mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Granulin-Antibody-OTI3H6-Immunohistochemistry-Paraffin-NBP2-45915-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Granulin Antibody [NBP1-87325] - Staining of human cell line A-431 shows localization to endoplasmic reticulum, endosomes & lysosomes. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Granulin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87325-img0004.jpg "Immunocytochemistry/Immunofluorescence: Granulin Antibody [NBP1-87325] - Staining of human cell line A-431 shows localization to endoplasmic reticulum, endosomes & lysosomes. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Granulin Antibody [NBP1-87325] - Staining of human spleen shows strong cytoplasmic positivity in cells in red pulp.](http://images.novusbio.com/fullsize/Granulin-Antibody-Immunohistochemistry-Paraffin-NBP1-87325-img0005.jpg)
![Immunohistochemistry-Paraffin: Granulin Antibody (CL5692) [NBP2-61424] - Staining of human skeletal muscle shows absence of positivity in striated muscle fibers as expected (negative control).](http://images.novusbio.com/fullsize/Granulin-Antibody-CL5692-Immunohistochemistry-Paraffin-NBP2-61424-img0004.jpg "Immunohistochemistry-Paraffin: Granulin Antibody (CL5692) [NBP2-61424] - Staining of human skeletal muscle shows absence of positivity in striated muscle fibers as expected (negative control).")
![Immunohistochemistry-Paraffin: Granulin Antibody (CL5692) [NBP2-61424] - Staining of human prostate shows moderate granular cytoplasmic immunoreactivity in glandular cells.](http://images.novusbio.com/fullsize/Granulin-Antibody-CL5692-Immunohistochemistry-Paraffin-NBP2-61424-img0001.jpg)
![Immunohistochemistry-Paraffin: Granulin Antibody (CL5692) [NBP2-61424] - Staining of human kidney shows cytoplasmic immunoreactivity in both renal glomeruli and tubules.](http://images.novusbio.com/fullsize/Granulin-Antibody-CL5692-Immunohistochemistry-Paraffin-NBP2-61424-img0002.jpg)
![Immunohistochemistry-Paraffin: Granulin Antibody (CL5692) [NBP2-61424] - Staining of human small intestine shows moderate granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Granulin-Antibody-CL5692-Immunohistochemistry-Paraffin-NBP2-61424-img0003.jpg)
![Western Blot: Granulin Antibody (1F5) [H00002896-M01] - GRN monoclonal antibody (M01), clone 1F5 Analysis of GRN expression in HeLa.](http://images.novusbio.com/fullsize/Granulin-Antibody-1F5-Western-Blot-H00002896-M01-img0007.jpg "Western Blot: Granulin Antibody (1F5) [H00002896-M01] - GRN monoclonal antibody (M01), clone 1F5 Analysis of GRN expression in HeLa.")
![Immunocytochemistry/Immunofluorescence: Granulin Antibody (1F5) [H00002896-M01] - Analysis of monoclonal antibody to GRN on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Granulin-Antibody-1F5-Immunocytochemistry-Immunofluorescence-H00002896-M01-img0005.jpg)
![Sandwich ELISA: Granulin Antibody (1F5) [H00002896-M01] - Detection limit for recombinant GST tagged GRN is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Granulin-Antibody-1F5-Sandwich-ELISA-H00002896-M01-img0006.jpg)
![Western Blot: Granulin Antibody (SR1971) [NBP3-22170] - Western blot analysis using NBP3-22170 in 293T cell lysate.](http://images.novusbio.com/fullsize/nbp3-22170_rabbit-granulin-mab-sr1971-572023152465.jpg "Western Blot: Granulin Antibody (SR1971) [NBP3-22170] - Western blot analysis using NBP3-22170 in 293T cell lysate.")
![Immunocytochemistry/Immunofluorescence: Granulin Antibody [NBP1-26396] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing ER/vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/Granulin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-26396-img0001.jpg "Immunocytochemistry/Immunofluorescence: Granulin Antibody [NBP1-26396] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing ER/vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).")
![Western Blot: Granulin Overexpression Lysate (Adult Normal) [NBL1-11353] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Granulin.](http://images.novusbio.com/fullsize/Granulin-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11353-img0002.jpg "Western Blot: Granulin Overexpression Lysate (Adult Normal) [NBL1-11353] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Granulin.")
![ELISA: Mouse Granulin ELISA Kit (Colorimetric) [NBP2-82199] - Samples were spiked with high concentrations of Mouse Granulin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-82199_mouse-granulin-elisa-kit-colorimetric-13220241625403.jpg "ELISA: Mouse Granulin ELISA Kit (Colorimetric) [NBP2-82199] - Samples were spiked with high concentrations of Mouse Granulin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Granulin ELISA Kit (Colorimetric) [NBP2-82199] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-82199_mouse-granulin-elisa-kit-colorimetric-432024955071.jpg)
![Western Blot: Granulin Antibody (4X3I7) [NBP3-16566] - Western blot analysis of extracts of A-431 cells, using Granulin Rabbit mAb (NBP3-16566) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Granulin-Antibody-4X3I7-Western-Blot-NBP3-16566-img0001.jpg "Western Blot: Granulin Antibody (4X3I7) [NBP3-16566] - Western blot analysis of extracts of A-431 cells, using Granulin Rabbit mAb (NBP3-16566) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")