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![Western Blot: EBAG9/RCAS1 Antibody [NB100-41096] - Staining of A431 lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence. (0.2ug/ml)](http://images.novusbio.com/fullsize/EBAG9-RCAS1-Antibody-Western-Blot-NB100-41096-img0001.jpg "Western Blot: EBAG9/RCAS1 Antibody [NB100-41096] - Staining of A431 lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence. (0.2ug/ml)")
![Immunohistochemistry-Paraffin: EBAG9/RCAS1 Antibody [NB100-41096] - staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/EBAG9-RCAS1-Antibody-Immunohistochemistry-Paraffin-NB100-41096-img0002.jpg)
![Immunohistochemistry-Paraffin: EBAG9/RCAS1 Antibody [NBP1-84922] - Staining of human liver.](http://images.novusbio.com/fullsize/EBAG9-RCAS1-Antibody-Immunohistochemistry-Paraffin-NBP1-84922-img0006.jpg "Immunohistochemistry-Paraffin: EBAG9/RCAS1 Antibody [NBP1-84922] - Staining of human liver.")
![Immunohistochemistry-Paraffin: EBAG9/RCAS1 Antibody [NBP1-84922] - Staining of human rectum shows strong cytoplasmic positivity, with a granular pattern, in glandular cells.](http://images.novusbio.com/fullsize/EBAG9-RCAS1-Antibody-Immunohistochemistry-Paraffin-NBP1-84922-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: EBAG9/RCAS1 Antibody [NBP1-84922] - Staining of human cerebral cortex, colon, liver and lymph node using Anti-EBAG9 antibody NBP1-84922 (A) shows similar protein distribution across tissues to independent antibody NBP1-84923 (B).](http://images.novusbio.com/fullsize/EBAG9-RCAS1-Antibody-Immunohistochemistry-Paraffin-NBP1-84922-img0003.jpg)
![Immunohistochemistry-Paraffin: EBAG9/RCAS1 Antibody [NBP1-84922] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/EBAG9-RCAS1-Antibody-Immunohistochemistry-Paraffin-NBP1-84922-img0004.jpg)
![Immunohistochemistry-Paraffin: EBAG9/RCAS1 Antibody [NBP1-84922] - Staining of human colon.](http://images.novusbio.com/fullsize/EBAG9-RCAS1-Antibody-Immunohistochemistry-Paraffin-NBP1-84922-img0005.jpg)
![Immunohistochemistry-Paraffin: EBAG9/RCAS1 Antibody [NBP1-84922] - Staining of human lymph node.](http://images.novusbio.com/fullsize/EBAG9-RCAS1-Antibody-Immunohistochemistry-Paraffin-NBP1-84922-img0007.jpg)
![Western Blot: EBAG9/RCAS1 Antibody [NBP3-00242] - Anti-EBAG9/RCAS1 rabbit polyclonal antibody at 1:500 dilution. Lane A: A431 Whole Cell Lysate. Lane B: MCF7 Whole Cell Lysate. Lane C: THP1 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 24 kDa. Observed band size: 37 kDa](http://images.novusbio.com/fullsize/EBAG9-RCAS1-Antibody-Western-Blot-NBP3-00242-img0007.jpg "Western Blot: EBAG9/RCAS1 Antibody [NBP3-00242] - Anti-EBAG9/RCAS1 rabbit polyclonal antibody at 1:500 dilution. Lane A: A431 Whole Cell Lysate. Lane B: MCF7 Whole Cell Lysate. Lane C: THP1 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 24 kDa. Observed band size: 37 kDa")
![Immunohistochemistry-Paraffin: EBAG9/RCAS1 Antibody [NBP3-00242] - Immunochemical staining of human EBAG9 in human heart with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/EBAG9-RCAS1-Antibody-Immunohistochemistry-Paraffin-NBP3-00242-img0005.jpg)
![Immunohistochemistry-Paraffin: EBAG9/RCAS1 Antibody [NBP3-00242] - Immunochemical staining of human EBAG9/RCAS1 in human prostate with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/EBAG9-RCAS1-Antibody-Immunohistochemistry-Paraffin-NBP3-00242-img0003.jpg)
![Immunohistochemistry-Paraffin: EBAG9/RCAS1 Antibody [NBP3-00242] - Immunochemical staining of human EBAG9/RCAS1 in human skeletal muscle with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/EBAG9-RCAS1-Antibody-Immunohistochemistry-Paraffin-NBP3-00242-img0004.jpg)
![Immunoprecipitation: EBAG9/RCAS1 Antibody [NBP3-00242] - EBAG9/RCAS1 was immunoprecipitated using: Lane A: 0.5 mg MCF-7 Whole Cell Lysate. 2 ul anti-EBAG9/RCAS1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-EBAG9/RCAS1 rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 37 kDa. Observed band size: 37 kDa](http://images.novusbio.com/fullsize/EBAG9-RCAS1-Antibody-Immunoprecipitation-NBP3-00242-img0006.jpg)
![Immunohistochemistry-Paraffin: EBAG9/RCAS1 Antibody [NBP1-84923] - Staining of human lymph node.](http://images.novusbio.com/fullsize/EBAG9-RCAS1-Antibody-Immunohistochemistry-Paraffin-NBP1-84923-img0005.jpg "Immunohistochemistry-Paraffin: EBAG9/RCAS1 Antibody [NBP1-84923] - Staining of human lymph node.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: EBAG9/RCAS1 Antibody [NBP1-84923] - Staining of human cerebral cortex, colon, liver and lymph node using Anti-EBAG9 antibody NBP1-84923 (A) shows similar protein distribution across tissues to independent antibody NBP1-84922 (B).](http://images.novusbio.com/fullsize/EBAG9-RCAS1-Antibody-Immunohistochemistry-Paraffin-NBP1-84923-img0002.jpg)
![Immunohistochemistry-Paraffin: EBAG9/RCAS1 Antibody [NBP1-84923] - Staining of human liver.](http://images.novusbio.com/fullsize/EBAG9-RCAS1-Antibody-Immunohistochemistry-Paraffin-NBP1-84923-img0003.jpg)
![Immunohistochemistry-Paraffin: EBAG9/RCAS1 Antibody [NBP1-84923] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/EBAG9-RCAS1-Antibody-Immunohistochemistry-Paraffin-NBP1-84923-img0004.jpg)
![Immunohistochemistry-Paraffin: EBAG9/RCAS1 Antibody [NBP1-84923] - Staining of human colon.](http://images.novusbio.com/fullsize/EBAG9-RCAS1-Antibody-Immunohistochemistry-Paraffin-NBP1-84923-img0006.jpg)
![Western Blot: EBAG9/RCAS1 Antibody (4A10) [H00009166-M04] - Analysis of EBAG9 expression in HepG2.](http://images.novusbio.com/fullsize/EBAG9-RCAS1-Antibody-4A10-Western-Blot-H00009166-M04-img0003.jpg "Western Blot: EBAG9/RCAS1 Antibody (4A10) [H00009166-M04] - Analysis of EBAG9 expression in HepG2.")
![Western Blot: EBAG9/RCAS1 Antibody (4A10) [H00009166-M04] - EBAG9 monoclonal antibody (M04), clone 4A10. Western Blot analysis of EBAG9 expression in MCF-7 ( Cat # L046V1 ).](http://images.novusbio.com/fullsize/EBAG9-RCAS1-Antibody-4A10-Western-Blot-H00009166-M04-img0001.jpg)
![Western Blot: EBAG9/RCAS1 Antibody (4A10) [H00009166-M04] - Analysis of EBAG9 expression in transfected 293T cell line by EBAG9 monoclonal antibody (M04), clone 4A10. Lane 1: EBAG9 transfected lysate (Predicted MW: 23.54 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/EBAG9-RCAS1-Antibody-4A10-Western-Blot-H00009166-M04-img0004.jpg)
![Sandwich ELISA: EBAG9/RCAS1 Antibody (4A10) [H00009166-M04] - Detection limit for recombinant GST tagged EBAG9 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/EBAG9-RCAS1-Antibody-4A10-Sandwich-ELISA-H00009166-M04-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: EBAG9/RCAS1 Antibody (CPTC-EBAG9-1) [NBP3-07534] - Immunofluorescence Analysis of Human MCF-7 cells labeling EBAG9/RCAS1 with EBAG9/RCAS1 Mouse Monoclonal Antibody (CPTC-EBAG9-1) followed by goat anti- Mouse IgG-CF488 (Green). The nuclear counterstain is RedDot (Red).](http://images.novusbio.com/fullsize/EBAG9-RCAS1-Antibody-CPTC-EBAG9-1-Immunocytochemistry-Immunofluorescence-NBP3-07534-img0001.jpg "Immunocytochemistry/Immunofluorescence: EBAG9/RCAS1 Antibody (CPTC-EBAG9-1) [NBP3-07534] - Immunofluorescence Analysis of Human MCF-7 cells labeling EBAG9/RCAS1 with EBAG9/RCAS1 Mouse Monoclonal Antibody (CPTC-EBAG9-1) followed by goat anti- Mouse IgG-CF488 (Green). The nuclear counterstain is RedDot (Red).")
![Immunohistochemistry-Paraffin: EBAG9/RCAS1 Antibody (CPTC-EBAG9-1) [NBP3-07534] - Formalin-fixed, paraffin-embedded human Unknown Tumor stained with EBAG9/RCAS1 Mouse Monoclonal Antibody (CPTC-EBAG9-1).](http://images.novusbio.com/fullsize/EBAG9-RCAS1-Antibody-CPTC-EBAG9-1-Immunohistochemistry-Paraffin-NBP3-07534-img0002.jpg)
![Western Blot: EBAG9/RCAS1 Antibody (CPTC-EBAG9-2) [NBP3-07535] - Western Blot Analysis of HEK293 cell lysate using EBAG9/RCAS1 Mouse Monoclonal Antibody (CPTC-EBAG9-1).](http://images.novusbio.com/fullsize/EBAG9-RCAS1-Antibody-CPTC-EBAG9-2-Western-Blot-NBP3-07535-img0002.jpg "Western Blot: EBAG9/RCAS1 Antibody (CPTC-EBAG9-2) [NBP3-07535] - Western Blot Analysis of HEK293 cell lysate using EBAG9/RCAS1 Mouse Monoclonal Antibody (CPTC-EBAG9-1).")
![Immunohistochemistry-Paraffin: EBAG9/RCAS1 Antibody (CPTC-EBAG9-2) [NBP3-07535] - Formalin-fixed, paraffin-embedded human Tonsil stained with EBAG9/RCAS1 Mouse Monoclonal Antibody (CPTC-EBAG9-2).](http://images.novusbio.com/fullsize/EBAG9-RCAS1-Antibody-CPTC-EBAG9-2-Immunohistochemistry-Paraffin-NBP3-07535-img0001.jpg)
![Western Blot: EBAG9/RCAS1 Antibody [NBP1-47292] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RCAS1 antibody NBP1-47292 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. RCAS1 was also immunoprecipitated by rabbit anti-RCAS1 antibody NBP1-47291, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).](http://images.novusbio.com/fullsize/EBAG9-RCAS1-Antibody-Western-Blot-NBP1-47292-img0001.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: EBAG9/RCAS1 Antibody [NBP1-47292] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RCAS1 antibody NBP1-47292 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. RCAS1 was also immunoprecipitated by rabbit anti-RCAS1 antibody NBP1-47291, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).")
![Western Blot: EBAG9/RCAS1 Overexpression Lysate (Adult Normal) [NBP2-04739] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for EBAG9/RCAS1.](http://images.novusbio.com/fullsize/EBAG9-RCAS1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04739-img0001.jpg "Western Blot: EBAG9/RCAS1 Overexpression Lysate (Adult Normal) [NBP2-04739] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for EBAG9/RCAS1.")
![Western Blot: EBAG9/RCAS1 Overexpression Lysate (Adult Normal) [NBL1-10090] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for EBAG9/RCAS1.](http://images.novusbio.com/fullsize/EBAG9-RCAS1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-10090-img0002.jpg "Western Blot: EBAG9/RCAS1 Overexpression Lysate (Adult Normal) [NBL1-10090] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for EBAG9/RCAS1.")
![Western Blot: EBAG9/RCAS1 Antibody (019) [NBP2-90266] - Anti-EBAG9 rabbit monoclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: MCF7 Whole Cell Lysate. Lane C: A431 Whole Cell Lysate. Lysates/proteins at 30 ug per lane.](http://images.novusbio.com/fullsize/EBAG9-RCAS1-Antibody-019-Western-Blot-NBP2-90266-img0002.jpg "Western Blot: EBAG9/RCAS1 Antibody (019) [NBP2-90266] - Anti-EBAG9 rabbit monoclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: MCF7 Whole Cell Lysate. Lane C: A431 Whole Cell Lysate. Lysates/proteins at 30 ug per lane.")
