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![Immunocytochemistry/Immunofluorescence: Dicer Antibody [NBP1-71691] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Dicer at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Dicer-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-71691-img0008.jpg "Immunocytochemistry/Immunofluorescence: Dicer Antibody [NBP1-71691] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Dicer at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Immunohistochemistry-Paraffin: Dicer Antibody [NBP1-71691] - Analysis of Dicer in paraffin embedded human colorectal cancer tissue using anti-Dicer antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Dicer-Antibody-Immunohistochemistry-Paraffin-NBP1-71691-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Dicer Antibody [NBP1-71691] - Dicer antibody was tested in HeLa cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Dicer-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-71691-img0005.jpg)
![Immunohistochemistry: Dicer Antibody [NBP1-71691] - Staining of Dicer in mouse prostate using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Dicer-Antibody-Immunohistochemistry-NBP1-71691-img0006.jpg)
![Western Blot: Dicer Antibody [NBP1-06520] - Whole cell lysate (15 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiDicer antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Dicer-Antibody-Western-Blot-NBP1-06520-img0003.jpg "Western Blot: Dicer Antibody [NBP1-06520] - Whole cell lysate (15 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiDicer antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunoprecipitation: Dicer Antibody [NBP1-06520] - Detection of human Dicer by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Dicer antibody NBP1-06520 (lot NBP1-06520-5) used for IP at 6 ug per reaction. Dicer was also immunoprecipitated by a previous lot of this antibody (lot NBP1-06520-4). For blotting immunoprecipitated Dicer, NBP1-06520 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/Dicer-Antibody-Immunoprecipitation-NBP1-06520-img0004.jpg)
![Western Blot: Dicer Antibody (CL0378) [NBP2-30699] - Analysis of extracts from RT-4 cells, transfected with either control siRNA (siRNA ctrl) or target specific siRNA probes (siRNA#1, siRNA#2), using Anti-DICER monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.](http://images.novusbio.com/fullsize/Dicer-Antibody-CL0378-Western-Blot-NBP2-30699-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Dicer Antibody (CL0378) [NBP2-30699] - Analysis of extracts from RT-4 cells, transfected with either control siRNA (siRNA ctrl) or target specific siRNA probes (siRNA#1, siRNA#2), using Anti-DICER monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Dicer Antibody (CL0378) [NBP2-30699] - Staining of A-431 cells using the anti-DICER1 monoclonal antibody, showing specific staining in the cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Dicer-Antibody-CL0378-Immunocytochemistry-Immunofluorescence-NBP2-30699-img0018.jpg)
![Immunohistochemistry-Paraffin: Dicer Antibody (CL0378) [NBP2-30699] - Staining of human breast cancer (ductal carcinoma) shows moderate cytoplasmic positivity in cancer cells.](http://images.novusbio.com/fullsize/Dicer-Antibody-CL0378-Immunohistochemistry-Paraffin-NBP2-30699-img0017.jpg)
![Western Blot: Dicer Antibody (CL0378) [NBP2-30699] - Lane 1: Marker [kDa]. Lane 2: Human cell line HEK293T](http://images.novusbio.com/fullsize/Dicer-Antibody-CL0378-Western-Blot-NBP2-30699-img0008.jpg)
![Immunohistochemistry-Paraffin: Dicer Antibody (CL0378) [NBP2-30699] - Staining of human uterus shows cytoplasmic immunoreactivity in the glandular epithelium cells.](http://images.novusbio.com/fullsize/Dicer-Antibody-CL0378-Immunohistochemistry-Paraffin-NBP2-30699-img0011.jpg)
![Immunohistochemistry-Paraffin: Dicer Antibody (CL0378) [NBP2-30699] - Staining of human testis shows cytoplasmic positivity in the seminiferous tubules cells.](http://images.novusbio.com/fullsize/Dicer-Antibody-CL0378-Immunohistochemistry-Paraffin-NBP2-30699-img0012.jpg)
![Immunohistochemistry-Paraffin: Dicer Antibody (CL0378) [NBP2-30699] - Staining of human prostate shows cytoplasmic immunoreactivity in the glandular epithelium cells.](http://images.novusbio.com/fullsize/Dicer-Antibody-CL0378-Immunohistochemistry-Paraffin-NBP2-30699-img0013.jpg)
![Immunohistochemistry-Paraffin: Dicer Antibody (CL0378) [NBP2-30699] - Staining of human cerebral cortex shows cytoplasmic positivity in the glial cells.](http://images.novusbio.com/fullsize/Dicer-Antibody-CL0378-Immunohistochemistry-Paraffin-NBP2-30699-img0014.jpg)
![Immunohistochemistry-Paraffin: Dicer Antibody (CL0378) [NBP2-30699] - Staining of human colorectal cancer (adenocarcinoma) shows cytoplasmic positivity in cancer cells.](http://images.novusbio.com/fullsize/Dicer-Antibody-CL0378-Immunohistochemistry-Paraffin-NBP2-30699-img0015.jpg)
![Immunohistochemistry-Paraffin: Dicer Antibody (CL0378) [NBP2-30699] - Staining of human lung cancer (adenocarcinoma) shows strong cytoplasmic positivity in cancer cells.](http://images.novusbio.com/fullsize/Dicer-Antibody-CL0378-Immunohistochemistry-Paraffin-NBP2-30699-img0016.jpg)
![Knockdown Validated: Dicer Antibody (HL1232) - Azide and BSA Free [NBP3-25439] - Non-transfected (-) and transfected (+) MCF-7 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody [HL1232] (NBP3-25439) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25439_rabbit-dicer-mab-hl1232-azide-and-bsa-free-2120249403838.jpg "<b>Genetic Strategies Validation. </b>Knockdown Validated: Dicer Antibody (HL1232) - Azide and BSA Free [NBP3-25439] - Non-transfected (-) and transfected (+) MCF-7 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody [HL1232] (NBP3-25439) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: Dicer Antibody (HL1232) - Azide and BSA Free [NBP3-25439] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody [HL1232] (NBP3-25439) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25439_rabbit-dicer-mab-hl1232-azide-and-bsa-free-2120249393545.jpg)
![Western Blot: Dicer Antibody (HL1232) - Azide and BSA Free [NBP3-25439] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody [HL1232] (NBP3-25439) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25439_rabbit-dicer-mab-hl1232-azide-and-bsa-free-2120249432868.jpg)
![Immunocytochemistry/Immunofluorescence: Dicer Antibody (HL1232) - Azide and BSA Free [NBP3-25439] - Dicer antibody [HL1232] detects Dicer protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Dicer stained by Dicer antibody [HL1232] (NBP3-25439) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI . Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp3-25439_rabbit-dicer-mab-hl1232-azide-and-bsa-free-2120249362777.jpg)
![Western Blot: Dicer Antibody (HL1232) - Azide and BSA Free [NBP3-25439] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody [HL1232] (NBP3-25439) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25439_rabbit-dicer-mab-hl1232-azide-and-bsa-free-212024939176.jpg)
![Immunohistochemistry-Paraffin: Dicer Antibody (HL1232) - Azide and BSA Free [NBP3-25439] - Dicer antibody [HL1232] detects Dicer protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. Dicer stained by Dicer antibody [HL1232] (NBP3-25439) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25439_rabbit-dicer-mab-hl1232-azide-and-bsa-free-2120249371566.jpg)
![Western Blot: Dicer Antibody (HL1232) - Azide and BSA Free [NBP3-25439] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody [HL1232] (NBP3-25439) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25439_rabbit-dicer-mab-hl1232-azide-and-bsa-free-2120249362730.jpg)
![Immunohistochemistry-Paraffin: Dicer Antibody [NBP1-85213] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Dicer-Antibody-Immunohistochemistry-Paraffin-NBP1-85213-img0007.jpg "Immunohistochemistry-Paraffin: Dicer Antibody [NBP1-85213] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.")
![Immunohistochemistry-Paraffin: Dicer Antibody [NBP1-85213] - Staining of human cerebellum shows moderate positivity in neuropil.](http://images.novusbio.com/fullsize/Dicer-Antibody-Immunohistochemistry-Paraffin-NBP1-85213-img0004.jpg)
![Immunohistochemistry-Paraffin: Dicer Antibody [NBP1-85213] - Staining of human endometrium shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Dicer-Antibody-Immunohistochemistry-Paraffin-NBP1-85213-img0005.jpg)
![Immunohistochemistry-Paraffin: Dicer Antibody [NBP1-85213] - Staining of human pancreas shows weak cytoplasmic positivity in islets of Langerhans, while exocrine cells are mainly negative.](http://images.novusbio.com/fullsize/Dicer-Antibody-Immunohistochemistry-Paraffin-NBP1-85213-img0006.jpg)
![Immunohistochemistry-Paraffin: Dicer Antibody [NBP2-23891] - Analysis of human testis using Dicer antibody at 1:200.](http://images.novusbio.com/fullsize/Dicer-Antibody-Immunohistochemistry-Paraffin-NBP2-23891-img0002.jpg "Immunohistochemistry-Paraffin: Dicer Antibody [NBP2-23891] - Analysis of human testis using Dicer antibody at 1:200.")
![Flow Cytometry: Dicer Antibody [NBP2-23891] - Analysis of HeLa cells transiently transfected with Dicer or control siRNA and analyzed for Dicer expression using this antibody. Purple represents isotype control staining.](http://images.novusbio.com/fullsize/Dicer-Antibody-Flow-Cytometry-NBP2-23891-img0003.jpg)
![ELISA: Dicer Antibody [NBP2-23891] - ELISA assay of HeLa cell lysate transiently transfected with Dicer or control siRNA and analyzed for Dicer expression using Dicer antibody followed by an anti rabbit IgG-HRP antibody.](http://images.novusbio.com/fullsize/Dicer-Antibody-ELISA-NBP2-23891-img0001.jpg)
![Western Blot: Dicer Antibody [NBP2-88949] - Total protein from human HeLa and A431 cells, mouse 3T3 cells and Rat PC12 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Dicer in block buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).](http://images.novusbio.com/fullsize/Dicer-Antibody-Western-Blot-NBP2-88949-img0002.jpg "Western Blot: Dicer Antibody [NBP2-88949] - Total protein from human HeLa and A431 cells, mouse 3T3 cells and Rat PC12 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Dicer in block buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).")
![Immunohistochemistry-Paraffin: Dicer Antibody [NBP2-88949] - Analysis of a FFPE tissue section of the mouse prostate using 1:200 dilution of Dicer antibody (NBP2-88949). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated moderate staining of cell cytoplasm.](http://images.novusbio.com/fullsize/Dicer-Antibody-Immunohistochemistry-Paraffin-NBP2-88949-img0001.jpg)
![Western Blot: Dicer Antibody [NBP1-06521] - Whole cell lysate from 293T and HeLa cells. Dicer was also immunoprecipitated by rabbit anti-Dicer antibodies NBP1-06520 and another Dicer antibody.](http://images.novusbio.com/fullsize/Dicer-Antibody-Western-Blot-NBP1-06521-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Dicer Antibody [NBP1-06521] - Whole cell lysate from 293T and HeLa cells. Dicer was also immunoprecipitated by rabbit anti-Dicer antibodies NBP1-06520 and another Dicer antibody.")
![Western Blot: Dicer Antibody (S167-7) [NBP2-22415] - Western Blot analysis of Rat Brain Membrane showing detection of ~215 kDa Dicer protein using Mouse Anti-Dicer Monoclonal Antibody, Clone S167-7 (NBP2-22415). Lane 1: MW Ladder. Lane 2: Rat Brain Membrane. Load: 10 ug. Block: 5% milk. Primary Antibody: Mouse Anti-Dicer Monoclonal Antibody (NBP2-22415) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~215 kDa.](http://images.novusbio.com/fullsize/Dicer-Antibody-S167-7-Western-Blot-NBP2-22415-img0004.jpg "Western Blot: Dicer Antibody (S167-7) [NBP2-22415] - Western Blot analysis of Rat Brain Membrane showing detection of ~215 kDa Dicer protein using Mouse Anti-Dicer Monoclonal Antibody, Clone S167-7 (NBP2-22415). Lane 1: MW Ladder. Lane 2: Rat Brain Membrane. Load: 10 ug. Block: 5% milk. Primary Antibody: Mouse Anti-Dicer Monoclonal Antibody (NBP2-22415) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~215 kDa.")
![Immunocytochemistry/Immunofluorescence: Dicer Antibody (S167-7) [NBP2-22415] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Dicer Monoclonal Antibody, Clone S167-7 (NBP2-22415). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Dicer Monoclonal Antibody (NBP2-22415) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Dicer Antibody (D) Composite.](http://images.novusbio.com/fullsize/Dicer-Antibody-S167-7-Immunocytochemistry-Immunofluorescence-NBP2-22415-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Dicer Antibody (S167-7) [NBP2-22415] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Dicer Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Dicer Antibody (D) Composite.](http://images.novusbio.com/fullsize/Dicer-Antibody-S167-7-Immunocytochemistry-Immunofluorescence-NBP2-22415-img0003.jpg)
![Western Blot: Dicer Antibody (GT1176) [NBP3-13572] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody [GT1176] (NBP3-13572) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Dicer-Antibody-GT1176-Western-Blot-NBP3-13572-img0001.jpg "Western Blot: Dicer Antibody (GT1176) [NBP3-13572] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody [GT1176] (NBP3-13572) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Dicer Antibody (GT1176) [NBP3-13572] - Dicer antibody [GT1176] detects Dicer protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Dicer stained by Dicer antibody [GT1176] (NBP3-13572) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10um.](http://images.novusbio.com/fullsize/Dicer-Antibody-GT1176-Immunocytochemistry-Immunofluorescence-NBP3-13572-img0002.jpg)
![Recombinant Human Dicer Protein [H00023405-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00023405-Q01-1.jpg "Recombinant Human Dicer Protein [H00023405-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")