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![Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-85358] - Staining in human thyroid gland and skeletal muscle tissues using anti-PPIB antibody. Corresponding PPIB RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-Immunohistochemistry-Paraffin-NBP1-85358-img0013.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-85358] - Staining in human thyroid gland and skeletal muscle tissues using anti-PPIB antibody. Corresponding PPIB RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: Cyclophilin B Antibody [NBP1-85358] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-Western-Blot-NBP1-85358-img0009.jpg)
![Western Blot: Cyclophilin B Antibody [NBP1-85358] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-Western-Blot-NBP1-85358-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: Cyclophilin B Antibody [NBP1-85358] - Staining of human cell line HUVEC TERT2 shows localization to endoplasmic reticulum. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85358-img0007.jpg)
![Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-85358] - Staining of human placenta shows strong granular cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-Immunohistochemistry-Paraffin-NBP1-85358-img0017.jpg)
![Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-85358] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-Immunohistochemistry-Paraffin-NBP1-85358-img0010.jpg)
![Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-85358] - Staining of human thyroid gland shows high expression.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-Immunohistochemistry-Paraffin-NBP1-85358-img0011.jpg)
![Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-85358] - Staining of human prostate shows weak granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-Immunohistochemistry-Paraffin-NBP1-85358-img0015.jpg)
![Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-85358] - Staining of human endometrium shows moderate granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-Immunohistochemistry-Paraffin-NBP1-85358-img0016.jpg)
![Immunohistochemistry-Paraffin: Cyclophilin B Antibody (CL3901) [NBP2-59781] - Analysis in human placenta and skeletal muscle tissues using NBP2-59781 antibody. Corresponding PPIB RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-CL3901-Immunohistochemistry-Paraffin-NBP2-59781-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Cyclophilin B Antibody (CL3901) [NBP2-59781] - Analysis in human placenta and skeletal muscle tissues using NBP2-59781 antibody. Corresponding PPIB RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: Cyclophilin B Antibody (CL3901) [NBP2-59781] - Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-PPIB monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-CL3901-Western-Blot-NBP2-59781-img0009.jpg)
![Immunohistochemistry-Paraffin: Cyclophilin B Antibody (CL3901) [NBP2-59781] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-CL3901-Immunohistochemistry-Paraffin-NBP2-59781-img0014.jpg)
![Western Blot: Cyclophilin B Antibody (CL3901) [NBP2-59781] - Analysis in human cell line HeLa, human cell line HEK 293, human cell line A-431, human cell line HepG2, mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-CL3901-Western-Blot-NBP2-59781-img0010.jpg)
![Immunohistochemistry-Paraffin: Cyclophilin B Antibody (CL3901) [NBP2-59781] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-CL3901-Immunohistochemistry-Paraffin-NBP2-59781-img0012.jpg)
![Immunohistochemistry-Paraffin: Cyclophilin B Antibody (CL3901) [NBP2-59781] - Staining of human prostate shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-CL3901-Immunohistochemistry-Paraffin-NBP2-59781-img0013.jpg)
![Immunohistochemistry-Paraffin: Cyclophilin B Antibody (CL3901) [NBP2-59781] - Staining of human skeletal muscle shows negative to very weak cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-CL3901-Immunohistochemistry-Paraffin-NBP2-59781-img0015.jpg)
![Knockout Validated: Cyclophilin B Antibody [NBP2-24770] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Cyclophilin B knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human Cyclophilin B Polyclonal Antibody (Catalog # NBP2-24770) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Cyclophilin B at approximately 22 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-Knockout-Validated-NBP2-24770-img0002.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: Cyclophilin B Antibody [NBP2-24770] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Cyclophilin B knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human Cyclophilin B Polyclonal Antibody (Catalog # NBP2-24770) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Cyclophilin B at approximately 22 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: Cyclophilin B Antibody [NBP2-24770] - analysis of Cyclophilin B in A) HeLa cell lysate and B) 293 cell lysate using this antibody. 5 and 2 ug/ml, respectively. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-Western-Blot-NBP2-24770-img0001.jpg)
![Western Blot: Cyclophilin B Antibody (5479) [NBP2-59782] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PPIB antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-5479-Western-Blot-NBP2-59782-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Cyclophilin B Antibody (5479) [NBP2-59782] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PPIB antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Western Blot: Cyclophilin B Antibody (5479) [NBP2-59782] - Analysis in human cell line HeLa, human cell line HEK 293, human cell line A-431, human cell line HepG2, mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-5479-Western-Blot-NBP2-59782-img0001.jpg)
![Western Blot: Cyclophilin B Antibody (M019) [NBP3-23271] - Western blot analysis of human LNCaP (lane 1), MCF7 (lane 2), MDA-MB-231 (lane 3), and MeWo (lane 4) cell lysates, as well as human recombinant full-length cyclophilin B (lane 5) and cyclophilin A (lane 6). The blot was probed with mouse monoclonal anti-cyclophilin B (NBP3-23271) at 1:500.](http://images.novusbio.com/fullsize/nbp3-23271_mouse-cyclophilin-b-mab-m019-199202318202910.jpg "Western Blot: Cyclophilin B Antibody (M019) [NBP3-23271] - Western blot analysis of human LNCaP (lane 1), MCF7 (lane 2), MDA-MB-231 (lane 3), and MeWo (lane 4) cell lysates, as well as human recombinant full-length cyclophilin B (lane 5) and cyclophilin A (lane 6). The blot was probed with mouse monoclonal anti-cyclophilin B (NBP3-23271) at 1:500.")
![Immunocytochemistry/Immunofluorescence: Cyclophilin B Antibody (M019) [NBP3-23271] - Immunocytochemical labeling of cyclophilin B in methanol:acetone (1:1) fixed human A549 lung cancer cells. The cells were labeled with mouse monoclonal anti-cyclophilin B (NBP3-23271). The antibody was detected using goat anti-mouse DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23271_mouse-cyclophilin-b-mab-m019-18920231735529.jpg)
![Western Blot: Cyclophilin B Antibody [NBP1-62356] - HepG2 cell lysate, antibody titration 1.25ug/ml. Gel concentration 12%](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-Western-Blot-NBP1-62356-img0004.jpg "Western Blot: Cyclophilin B Antibody [NBP1-62356] - HepG2 cell lysate, antibody titration 1.25ug/ml. Gel concentration 12%")
![Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-62356] - Human Liver Tissue, antibody concentration 4-8ug/ml. Cells with positive label: Hepatocytes (indicated with arrows) 400X magnification.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-Immunohistochemistry-Paraffin-NBP1-62356-img0003.jpg)
![SDS-Page: Recombinant Human Cyclophilin B Protein [NBC1-18424]](http://images.novusbio.com/fullsize/Recombinant-Human-Cyclophilin-B-Protein-SDS-Page-NBC1-18424-img0002.jpg "SDS-Page: Recombinant Human Cyclophilin B Protein [NBC1-18424]")
![Western Blot: Cyclophilin B Antibody [NBP2-98906] - Lane A: Hela whole cell lysateLane B: A431 whole cell lysateLane C: HepG2 whole cell lysateLane D: 293 whole cell lysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:23 kDaObserved band size:19 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-Western-Blot-NBP2-98906-img0006.jpg "Western Blot: Cyclophilin B Antibody [NBP2-98906] - Lane A: Hela whole cell lysateLane B: A431 whole cell lysateLane C: HepG2 whole cell lysateLane D: 293 whole cell lysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:23 kDaObserved band size:19 kDa(We are unsure as to the identity of these extra bands.)")
![Western Blot: Cyclophilin B Antibody [NBP2-98906] - Anti-Cyclophilin B rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: A431 Whole Cell Lysate. Lane C: HepG2 Whole Cell Lysate. Lane D: 293 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 23 kDa. Observed band size: 19 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-Western-Blot-NBP2-98906-img0004.jpg)
![Immunoprecipitation: Cyclophilin B Antibody [NBP2-98906] - Lane A:0.5 mg Hela Whole Cell Lysate2 uL anti-PPIB rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-PPIB rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 19 kDaObserved band size: 19 kDa](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-Immunoprecipitation-NBP2-98906-img0007.jpg)
![Western Blot: Cyclophilin B Antibody (7Z4S7) [NBP3-16494] - Western blot analysis of extracts of various cell lines, using Cyclophilin B Rabbit mAb (NBP3-16494) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-7Z4S7-Western-Blot-NBP3-16494-img0001.jpg "Western Blot: Cyclophilin B Antibody (7Z4S7) [NBP3-16494] - Western blot analysis of extracts of various cell lines, using Cyclophilin B Rabbit mAb (NBP3-16494) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: Cyclophilin B Antibody (S01-2F8) [NBP3-14922] - Western blot detection of Cyclophilin B in Hela, C6, Rat Brain lysates using Cyclophilin B Rabbit mAb (1:500 diluted). Predicted band size: 24kDa. Observed band size: 20kDa.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-S01-2F8-Western-Blot-NBP3-14922-img0001.jpg "Western Blot: Cyclophilin B Antibody (S01-2F8) [NBP3-14922] - Western blot detection of Cyclophilin B in Hela, C6, Rat Brain lysates using Cyclophilin B Rabbit mAb (1:500 diluted). Predicted band size: 24kDa. Observed band size: 20kDa.")