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![Flow (Intracellular): Complement C3 Antibody (11H9) [NB200-540] - An intracellular stain was performed on RAW 246.7 cells with Complement C3 (11H9-3-2) antibody NB200-540 (blue) and a matched isotype control NBP2-31382 (orange). Cells were either treated with 3uM Monensin for 3 hours to block the secretion of Complement C3 (B) or grown in normal media (A). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) PE-conjugated secondary antibody (F0102B, R&D Systems).](http://images.novusbio.com/fullsize/Complement-C3-Antibody-11H9-Flow-Intracellular-NB200-540-img0005.jpg "<b>Biological Strategies Validation. </b>Flow (Intracellular): Complement C3 Antibody (11H9) [NB200-540] - An intracellular stain was performed on RAW 246.7 cells with Complement C3 (11H9-3-2) antibody NB200-540 (blue) and a matched isotype control NBP2-31382 (orange). Cells were either treated with 3uM Monensin for 3 hours to block the secretion of Complement C3 (B) or grown in normal media (A). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) PE-conjugated secondary antibody (F0102B, R&D Systems).")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Complement C3 Antibody (11H9) [NB200-540] - C3 protein fragments deposited on kidney cells of MPL-lpr mouse. Staining with antibody 11H9. Glomerular staining pattern. Fixation in 4% paraformaldehyde in PBS pH 7.4. Vibratome sections of 4 um. Pretreated with 3% hydrogen peroxide for 20 min to quench endogenous peroxidases. Microwaved in antigen unmasking solution for 2-5 minutes as antigen retrieval.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-11H9-Immunocytochemistry-Immunofluorescence-NB200-540-img0002.jpg)
![Immunohistochemistry-Paraffin: Complement C3 Antibody (11H9) [NB200-540] - Complement C3 protein in a FFPE tissue section of mouse liver using 1:100 dilution of Complement C3 antibody (clone 11H9) NB200-540. Weak but distinct membrane-cytoplasmic immunopositivity was observed in hepatocytes and few cells developed punctate membrane staining.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-11H9-Immunohistochemistry-Paraffin-NB200-540-img0004.jpg)
![Flow Cytometry: Complement C3 Antibody (11H9) [NB200-540] - Left panel: FMO, Middle panel: No primary antibody control, Right panel: sample. Day 6 murine mammary tumors processed and stained for analysis with flow cytometry. The C3b+ population of CD45+ cells is what the gate in each sample is exhibiting. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-11H9-Flow-Cytometry-NB200-540-img0006.jpg)
![Immunohistochemistry-Paraffin: Complement C3 Antibody (11H9) [NB200-540] - Complement C3 protein in a FFPE tissue section of mouse lymph node using 1:100 dilution of Complement C3 antibody (clone 11H9) NB200-540. This representative photomicrograph shows a membrane-cytoplasmic immunopositivity in non-germinal center cells, and few cells developed an intense staining for this target protein.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-11H9-Immunohistochemistry-Paraffin-NB200-540-img0003.jpg)
![Western Blot: Complement C3 Antibody [NBP1-32080] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with C3 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-Western-Blot-NBP1-32080-img0023.jpg "Western Blot: Complement C3 Antibody [NBP1-32080] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with C3 antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Complement C3 Antibody [NBP1-32080] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: C3 protein stained by C3 antibody [C3], C-term diluted at 1:200. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32080-img0019.jpg)
![Immunohistochemistry-Paraffin: Complement C3 Antibody [NBP1-32080] - Mouse brain. C3 antibody [C3], C-term diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-Immunohistochemistry-Paraffin-NBP1-32080-img0015.jpg)
![Western Blot: Complement C3 Antibody [NBP1-32080] - Human plasma (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with C3 antibody [C3], C-term diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-Western-Blot-NBP1-32080-img0021.jpg)
![Simple Western: Complement C3 Antibody [NBP1-32080] - Simple Western lane view shows a specific band for Complement C3 in 0.05 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-Simple-Western-NBP1-32080-img0009.jpg)
![Immunoprecipitation: Complement C3 Antibody [NBP1-32080] - Immunoprecipitation of C3 protein from HepG2 whole cell extracts using 5 ug of C3 antibody [C3], C-term.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-Immunoprecipitation-NBP1-32080-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: Complement C3 Antibody (JF10-30) [NBP2-66994] - Staining C3 in NIH/3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100, PBS.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-JF10-30-Immunocytochemistry-Immunofluorescence-NBP2-66994-img0002.jpg "Immunocytochemistry/Immunofluorescence: Complement C3 Antibody (JF10-30) [NBP2-66994] - Staining C3 in NIH/3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100, PBS.")
![Flow Cytometry: Complement C3 Antibody (JF10-30) [NBP2-66994] - Analysis of HepG2 cells with C3 antibody at 1:50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/Complement-C3-Antibody-JF10-30-Flow-Cytometry-NBP2-66994-img0001.jpg)
![Flow Cytometry: Complement C3 Antibody (JF10-30) [NBP2-66994] - Cells were fixed and permeabilized. Then stained with the primary antibody (1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4c for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4 c. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp2-66994_rabbit-monoclonal-complement-c3-antibody-jf10-30-flow-cytometry-1542024105623..jpg)
![Western Blot: Complement C3 Antibody (JF10-30) [NBP2-66994] -Western blot analysis of C3 on different lysates with Rabbit anti-C3 antibody at 1/1,000 dilution.Lane 1: Human liver tissue lysateLane 2: HepG2 cell lysateLane 3: HeLa cell lysateLane 4: Mouse liver tissue lysateLane 5: Mouse spleen tissue lysateLane 6: Rat liver tissue lysateLane 7: Rat spleen tissue lysateLysates/proteins at 30 µg/Lane.Predicted band size: 187 kDaObserved band size: 108/37 kDaExposure time: 1 minute;4-20% SDS-PAGE gel.](http://images.novusbio.com/fullsize/nbp2-66994_rabbit-monoclonal-complement-c3-antibody-jf10-30-western-blot-1542024105739..jpg)
![Immunocytochemistry/Immunofluorescence: Complement C3 Antibody (JF10-30) [NBP2-66994] -Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-C3 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4 c. Goat Anti-Mouse IgG H&L (iFluor™ ) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp2-66994_rabbit-monoclonal-complement-c3-antibody-jf10-30-immunocytochemistry-immunofluorescence-15420241102..jpg)
![Immunohistochemistry-Paraffin: Complement C3 Antibody [NBP1-85492] - Staining of human adipose tissue, kidney, lung and testis using Anti-Complement C3 antibody NBP1-85492 (A) shows similar protein distribution across tissues to independent antibody NBP1-85493 (B).](http://images.novusbio.com/fullsize/Complement-C3-Antibody-Immunohistochemistry-Paraffin-NBP1-85492-img0007.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Complement C3 Antibody [NBP1-85492] - Staining of human adipose tissue, kidney, lung and testis using Anti-Complement C3 antibody NBP1-85492 (A) shows similar protein distribution across tissues to independent antibody NBP1-85493 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Complement C3 Antibody [NBP1-85492] - Staining of human adipose tissue shows moderate extracellular space positivity in adipocytes.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-Immunohistochemistry-Paraffin-NBP1-85492-img0001.jpg)
![Immunohistochemistry-Paraffin: Complement C3 Antibody [NBP1-85492] - Staining of human lung shows strong extracellular space positivity in macrophages.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-Immunohistochemistry-Paraffin-NBP1-85492-img0002.jpg)
![Immunohistochemistry-Paraffin: Complement C3 Antibody [NBP1-85492] - Staining of human testis shows strong extracellular space positivity in Leydig cells.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-Immunohistochemistry-Paraffin-NBP1-85492-img0003.jpg)
![Immunohistochemistry-Paraffin: Complement C3 Antibody [NBP1-85492] - Staining of human kidney shows strong extracellular space positivity in cells in tubules.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-Immunohistochemistry-Paraffin-NBP1-85492-img0004.jpg)
![Immunohistochemistry-Paraffin: Complement C3 Antibody [NBP1-85493] - Staining of human adipose tissue, kidney, lung and testis using Anti-Complement C3 antibody NBP1-85493 (A) shows similar protein distribution across tissues to independent antibody NBP1-85492 (B).](http://images.novusbio.com/fullsize/Complement-C3-Antibody-Immunohistochemistry-Paraffin-NBP1-85493-img0006.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Complement C3 Antibody [NBP1-85493] - Staining of human adipose tissue, kidney, lung and testis using Anti-Complement C3 antibody NBP1-85493 (A) shows similar protein distribution across tissues to independent antibody NBP1-85492 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Complement C3 Antibody [NBP1-85493] - Staining of human testis shows strong extracellular space positivity in Leydig cells.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-Immunohistochemistry-Paraffin-NBP1-85493-img0009.jpg)
![Immunohistochemistry-Paraffin: Complement C3 Antibody [NBP1-85493] - Staining of human adipose tissue shows strong extracellular space positivity in adipocytes.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-Immunohistochemistry-Paraffin-NBP1-85493-img0004.jpg)
![Immunohistochemistry-Paraffin: Complement C3 Antibody [NBP1-85493] - Staining of human kidney shows strong extracellular space positivity in cells in tubules.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-Immunohistochemistry-Paraffin-NBP1-85493-img0007.jpg)
![Immunohistochemistry-Paraffin: Complement C3 Antibody [NBP1-85493] - Staining of human lung shows strong extracellular space positivity in macrophages.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-Immunohistochemistry-Paraffin-NBP1-85493-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Complement C3 Antibody (HL1643) - Azide and BSA Free [NBP3-25389] - C3 antibody [HL1643] detects C3 protein by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: C3 stained by C3 antibody [HL1643] (NBP3-25389) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25389_rabbit-complement-c3-mab-hl1643-azide-and-bsa-free-2120249422664.jpg "Immunocytochemistry/Immunofluorescence: Complement C3 Antibody (HL1643) - Azide and BSA Free [NBP3-25389] - C3 antibody [HL1643] detects C3 protein by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: C3 stained by C3 antibody [HL1643] (NBP3-25389) diluted at 1:500. Blue: Fluoroshield with DAPI .")
![Western Blot: Complement C3 Antibody (HL1643) - Azide and BSA Free [NBP3-25389] - Human plasma (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with C3 antibody [HL1643] (NBP3-25389) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25389_rabbit-complement-c3-mab-hl1643-azide-and-bsa-free-2120249435517.jpg)
![Western Blot: Complement C3 Antibody (HL1643) - Azide and BSA Free [NBP3-25389] - Rat plasma (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with C3 antibody [HL1643] (NBP3-25389) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25389_rabbit-complement-c3-mab-hl1643-azide-and-bsa-free-2120249362747.jpg)
![Western Blot: Complement C3 Antibody (5F9) [H00000718-M01] - C3 monoclonal antibody (M01), clone 5F9 Analysis of C3 expression in A-431.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-5F9-Western-Blot-H00000718-M01-img0007.jpg "Western Blot: Complement C3 Antibody (5F9) [H00000718-M01] - C3 monoclonal antibody (M01), clone 5F9 Analysis of C3 expression in A-431.")
![Immunohistochemistry-Paraffin: Complement C3 Antibody (5F9) [H00000718-M01] - Analysis of monoclonal antibody to C3 on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration at 3 ug/mL.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-5F9-Immunohistochemistry-Paraffin-H00000718-M01-img0005.jpg)
![ELISA: Complement C3 Antibody (5F9) [H00000718-M01] - Detection limit for recombinant GST tagged C3 is approximately 1 ng/mL as a capture antibody.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-5F9-ELISA-H00000718-M01-img0008.jpg)
![Immunohistochemistry: Complement C3 Antibody (4D12) [NBP3-26471] - Image of Complement C3 Antibody (4D12) diluted at 1:100 and staining in paraffin-embedded human liver tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26471_rabbit-complement-c3-mab-4d12-262202415475630.jpg "Immunohistochemistry: Complement C3 Antibody (4D12) [NBP3-26471] - Image of Complement C3 Antibody (4D12) diluted at 1:100 and staining in paraffin-embedded human liver tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: Complement C3 Antibody (4D12) [NBP3-26471] - Positive Western Blot detected in: THP-1 whole cell lysate, K562 whole cell lysate. All lanes: Complement C3 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 188 kDa Observed band size: 115 kDa](http://images.novusbio.com/fullsize/nbp3-26471_rabbit-complement-c3-mab-4d12-28220241140917.jpg)
![Flow Cytometry: Complement C3 Antibody (4D12) [NBP3-26471] - Overlay histogram showing HepG2 cells stained with Complement C3 Antibody (4D12) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26471_rabbit-complement-c3-mab-4d12-282202414183687.jpg)
![SDS-Page: Complement C3 Partial Recombinant Protein [H00000718-Q01]](http://images.novusbio.com/fullsize/Complement-C3-Partial-Recombinant-Protein-SDS-Page-H00000718-Q01-img0001.jpg "SDS-Page: Complement C3 Partial Recombinant Protein [H00000718-Q01]")
![SDS-Page: Recombinant Mouse Complement C3 Protein [P3343] - 12.5% SDS-PAGE Stained with Coomassie Blue](http://images.novusbio.com/fullsize/Recombinant-Mouse-Complement-C3-Protein-SDS-Page-P3343-img0002.jpg "SDS-Page: Recombinant Mouse Complement C3 Protein [P3343] - 12.5% SDS-PAGE Stained with Coomassie Blue")
![ELISA: Human Complement C3 ELISA Kit (Colorimetric) [NBP2-60618]](http://images.novusbio.com/fullsize/Human-Complement-C3-ELISA-Kit-Colorimetric-ELISA-NBP2-60618-img0001.jpg "ELISA: Human Complement C3 ELISA Kit (Colorimetric) [NBP2-60618]")
![Western Blot: Complement C3 Antibody (S06-2C2) [NBP3-19586] - Western blot detection of Complement C3 in human serum lysates using NBP3-19586. Predicted band size: 187kDa. Observed band size: 187, 115, 40, 34kDa.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-S06-2C2-Western-Blot-NBP3-19586-img0001.jpg "Western Blot: Complement C3 Antibody (S06-2C2) [NBP3-19586] - Western blot detection of Complement C3 in human serum lysates using NBP3-19586. Predicted band size: 187kDa. Observed band size: 187, 115, 40, 34kDa.")