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![Western Blot: Coagulation Factor II/Thrombin Antibody [NBP1-58268] - 721_B tissue lysate at a concentration of 1ug/ml.](http://images.novusbio.com/fullsize/Coagulation-Factor-II-Thrombin-Antibody-Western-Blot-NBP1-58268-img0007.jpg "Western Blot: Coagulation Factor II/Thrombin Antibody [NBP1-58268] - 721_B tissue lysate at a concentration of 1ug/ml.")
![Immunohistochemistry: Coagulation Factor II/Thrombin Antibody [NBP1-58268] - Analysis of human liver after heat-induced Antigen retrieval. Antibody concentration 5 ug/ml.](http://images.novusbio.com/fullsize/Coagulation-Factor-II-Thrombin-Antibody-Immunohistochemistry-NBP1-58268-img0008.jpg)
![Immunohistochemistry-Paraffin: Coagulation Factor II/Thrombin Antibody [NBP1-58268] - Human Placenta Tissue](http://images.novusbio.com/fullsize/Coagulation-Factor-II-Thrombin-Antibody-Immunohistochemistry-Paraffin-NBP1-58268-img0005.jpg)
![Immunohistochemistry-Paraffin: Coagulation Factor II/Thrombin Antibody [NBP1-58268] - Human Placenta Tissue, 5.0ug/ml.](http://images.novusbio.com/fullsize/Coagulation-Factor-II-Thrombin-Antibody-Immunohistochemistry-Paraffin-NBP1-58268-img0006.jpg)
![Western Blot: Coagulation Factor II/Thrombin Antibody (1B7) [H00002147-M01] - Analysis of F2 expression in transfected 293T cell line by F2 monoclonal antibody (M01), clone 1B7.Lane 1: F2 transfected lysate(70 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Coagulation-Factor-II-Thrombin-Antibody-1B7-Western-Blot-H00002147-M01-img0008.jpg "Western Blot: Coagulation Factor II/Thrombin Antibody (1B7) [H00002147-M01] - Analysis of F2 expression in transfected 293T cell line by F2 monoclonal antibody (M01), clone 1B7.Lane 1: F2 transfected lysate(70 KDa).Lane 2: Non-transfected lysate.")
![Western Blot: Coagulation Factor II/Thrombin Antibody (1B7) [H00002147-M01] - Analysis of F2 over-expressed 293 cell line, cotransfected with F2 Validated Chimera RNAi ( Cat # H00002147-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with F2 monoclonal antibody (M01), clone 1B7 (Cat # H00002147-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/Coagulation-Factor-II-Thrombin-Antibody-1B7-Western-Blot-H00002147-M01-img0006.jpg)
![Proximity Ligation Assay: Coagulation Factor II/Thrombin Antibody (1B7) [H00002147-M01] - Analysis of protein-protein interactions between FGA and F2. HeLa cells were stained with anti-FGA rabbit purified polyclonal 1:1200 and anti-F2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Coagulation-Factor-II-Thrombin-Antibody-1B7-Proximity-Ligation-Assay-H00002147-M01-img0004.jpg)
![Immunoprecipitation: Coagulation Factor II/Thrombin Antibody (1B7) [H00002147-M01] - Analysis of F2 transfected lysate using anti-F2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with F2 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/Coagulation-Factor-II-Thrombin-Antibody-1B7-Immunoprecipitation-H00002147-M01-img0005.jpg)
![Sandwich ELISA: Coagulation Factor II/Thrombin Antibody (1B7) [H00002147-M01] - Detection limit for recombinant GST tagged F2 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Coagulation-Factor-II-Thrombin-Antibody-1B7-Sandwich-ELISA-H00002147-M01-img0007.jpg)
![Western Blot: F2 Antibody [NBP2-33617] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/F2-Antibody-Western-Blot-NBP2-33617-img0002.jpg "Western Blot: F2 Antibody [NBP2-33617] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: F2 Antibody [NBP2-33617] - Staining in human liver and pancreas tissues using anti-F2 antibody. Corresponding F2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/F2-Antibody-Immunohistochemistry-Paraffin-NBP2-33617-img0005.jpg)
![Immunohistochemistry-Paraffin: F2 Antibody [NBP2-33617] - Staining of human liver shows high expression.](http://images.novusbio.com/fullsize/F2-Antibody-Immunohistochemistry-Paraffin-NBP2-33617-img0003.jpg)
![Immunohistochemistry-Paraffin: F2 Antibody [NBP2-33617] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/F2-Antibody-Immunohistochemistry-Paraffin-NBP2-33617-img0004.jpg)
![Immunohistochemistry-Paraffin: Coagulation Factor II/Thrombin Antibody [NBP2-33728] - F2 Antibody [NBP2-33728] - Staining in human liver and pancreas tissues using anti-F2 antibody. Corresponding F2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/F2-Antibody-Immunohistochemistry-Paraffin-NBP2-33728-img0004.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Coagulation Factor II/Thrombin Antibody [NBP2-33728] - F2 Antibody [NBP2-33728] - Staining in human liver and pancreas tissues using anti-F2 antibody. Corresponding F2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: F2 Antibody [NBP2-33728] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/F2-Antibody-Immunohistochemistry-Paraffin-NBP2-33728-img0003.jpg)
![Immunohistochemistry-Paraffin: F2 Antibody [NBP2-33728] - Staining of human liver shows high expression.](http://images.novusbio.com/fullsize/F2-Antibody-Immunohistochemistry-Paraffin-NBP2-33728-img0002.jpg)
![Western Blot: Prothrombin Antibody [NBP1-74142] - Titration: 1.0 ug/ml Positive Control: Rat Lung.](http://images.novusbio.com/fullsize/Prothrombin-Antibody-Western-Blot-NBP1-74142-img0002.jpg "Western Blot: Prothrombin Antibody [NBP1-74142] - Titration: 1.0 ug/ml Positive Control: Rat Lung.")
![ELISA: Rat Coagulation Factor II/Thrombin ELISA Kit (Colorimetric) [NBP2-82491] - Samples were spiked with high concentrations of Rat Coagulation Factor II/Thrombin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-82491_rat-coagulation-factor-ii-thrombin-elisa-kit-colorimetric-132202416143040.jpg "ELISA: Rat Coagulation Factor II/Thrombin ELISA Kit (Colorimetric) [NBP2-82491] - Samples were spiked with high concentrations of Rat Coagulation Factor II/Thrombin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat Coagulation Factor II/Thrombin ELISA Kit (Colorimetric) [NBP2-82491] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-82491_rat-coagulation-factor-ii-thrombin-elisa-kit-colorimetric-432024855079.jpg)
![Western Blot: Coagulation Factor II/Thrombin Antibody (GMA-020) [NBP2-29813] - 2ug of human thrombin was probed with anti-Thrombin, clone GMA-020 (1ug/ml). Arrow indicates thrombin (non reduced) (~31kDa).](http://images.novusbio.com/fullsize/Coagulation-Factor-II-Thrombin-Antibody-GMA-020-Western-Blot-NBP2-29813-img0002.jpg "Western Blot: Coagulation Factor II/Thrombin Antibody (GMA-020) [NBP2-29813] - 2ug of human thrombin was probed with anti-Thrombin, clone GMA-020 (1ug/ml). Arrow indicates thrombin (non reduced) (~31kDa).")
![Western Blot: Coagulation Factor II/Thrombin Antibody (318) [NBP2-90543] - Lane A: Mouse kidney tissue lysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:70 kDaObserved band size:90 kDa](http://images.novusbio.com/fullsize/Coagulation-Factor-II-Thrombin-Antibody-318-Western-Blot-NBP2-90543-img0002.jpg "Western Blot: Coagulation Factor II/Thrombin Antibody (318) [NBP2-90543] - Lane A: Mouse kidney tissue lysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:70 kDaObserved band size:90 kDa")