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![Western Blot: Peripherin Antibody (7C5) [NB300-220] - Analysis of tissue and cell lysates using Peripherin antibody, dilution 1:1,000 (Green): [1] protein standard, [2] rat spinal cord, [3] rat sciatic nerve, [4] mouse spinal cord, [5] pig spinal cord, [6] cow spinal cord, [7] SH-SY5Y cells, and [8] PC12 cells. The band at ~57kDa corresponds to the peripherin protein detected only in rat and mouse lysates, since the antibody is rodent specific.](http://images.novusbio.com/fullsize/Peripherin-Antibody-7C5-Western-Blot-NB300-220-img0004.jpg "Western Blot: Peripherin Antibody (7C5) [NB300-220] - Analysis of tissue and cell lysates using Peripherin antibody, dilution 1:1,000 (Green): [1] protein standard, [2] rat spinal cord, [3] rat sciatic nerve, [4] mouse spinal cord, [5] pig spinal cord, [6] cow spinal cord, [7] SH-SY5Y cells, and [8] PC12 cells. The band at ~57kDa corresponds to the peripherin protein detected only in rat and mouse lysates, since the antibody is rodent specific.")
![Immunocytochemistry/Immunofluorescence: Peripherin Antibody (7C5) [NB300-220] - Analysis of mixed fibroblast and rat pheochromocytoma PC12 cell culture stained with peripherin antibody, dilution 1:500 (Red), and costained with chicken pAb to vimentin, dilution 1:10,000 (Green). Hoechst staining of nuclear DNA (Blue). The peripherin antibody reveals cytoplasmic filamentous staining in the small PC12 cells, while vimentin antibody stains intermediate filaments in the surrounding fibroblastic cells which do not express peripherin.](http://images.novusbio.com/fullsize/Peripherin-Antibody-7C5-Immunocytochemistry-Immunofluorescence-NB300-220-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Peripherin Antibody (7C5) [NB300-220] - Mixed neuron/glia cultures from newborn rat brain stained with NB300-220 antibody to peripherin (green) and rabbit polyclonal antibody to NF-L NBP1-05218 (red channel). A class of large neurons, like the one in the middle of this image, contain peripherin, while the majority of neurons and their processes contain NF-L and not peripherin. Interestingly, the periperin positive cells often contain a cytoplasmic inclusion next to the nucleus which stains for both peripherin and NF-L, and so appears golden in this kind of image. The blue channel reveals the localization of DNA.](http://images.novusbio.com/fullsize/Peripherin-Antibody-7C5-Immunocytochemistry-Immunofluorescence-NB300-220-img0003.jpg)
![Western Blot: Sulfotyrosine Antibody (7C5) [NBP2-59384] - Western Blot analysis of Sulfotyrosine-BSA Conjugate showing detection of 67 kDa Sulfotyrosine protein using Mouse Anti-Sulfotyrosine Monoclonal Antibody, Clone 7C5 (NBP2-59384). Lane 1: Molecular Weight Ladder (MW). Lane 2: Sulfotyrosine-BSA. Lane 3: Tyrosine-BSA. Lane 4: Phosphotyrosine-BSA. Lane 5: BSA. Load: 2 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Sulfotyrosine Monoclonal Antibody (NBP2-59384) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.](http://images.novusbio.com/fullsize/Sulfotyrosine-Antibody-7C5-Western-Blot-NBP2-59384-img0005.jpg "Western Blot: Sulfotyrosine Antibody (7C5) [NBP2-59384] - Western Blot analysis of Sulfotyrosine-BSA Conjugate showing detection of 67 kDa Sulfotyrosine protein using Mouse Anti-Sulfotyrosine Monoclonal Antibody, Clone 7C5 (NBP2-59384). Lane 1: Molecular Weight Ladder (MW). Lane 2: Sulfotyrosine-BSA. Lane 3: Tyrosine-BSA. Lane 4: Phosphotyrosine-BSA. Lane 5: BSA. Load: 2 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Sulfotyrosine Monoclonal Antibody (NBP2-59384) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.")
![Immunocytochemistry/Immunofluorescence: Sulfotyrosine Antibody (7C5) [NBP2-59384] - Analysis using Mouse Anti-Sulfotyrosine Monoclonal Antibody, Clone 7C5. Tissue: Embryonic kidney cells (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Sulfotyrosine Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Localization: Cytoplasmic. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) Sulfotyrosine Antibody (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.](http://images.novusbio.com/fullsize/Sulfotyrosine-Antibody-7C5-Immunocytochemistry-Immunofluorescence-NBP2-59384-img0004.jpg)
![Immunohistochemistry: Sulfotyrosine Antibody (7C5) [NBP2-59384] - Immunohistochemistry analysis using Mouse Anti-Sulfotyrosine Monoclonal Antibody, Clone 7C5 (NBP2-59384). Tissue: kidney. Species: Human. Fixation: Formalin fixed, paraffin embedded. Primary Antibody: Mouse Anti-Sulfotyrosine Monoclonal Antibody (NBP2-59384) at 1:25 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: Alexa Fluor 488. Counterstain: Actin-binding Phalloidin-Alexa Fluor 633; DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) Sulfotyrosine Antibody (D) Composite](http://images.novusbio.com/fullsize/Sulfotyrosine-Antibody-7C5-Immunohistochemistry-NBP2-59384-img0006.jpg)
![Immunohistochemistry-Paraffin: Paralemmin Antibody (7C5) [H00005064-M09] - Analysis of monoclonal antibody to PALM on formalin-fixed paraffin-embedded human kidney. Antibody concentration 1.5 ug/ml](http://images.novusbio.com/fullsize/Paralemmin-Antibody-7C5-Immunohistochemistry-Paraffin-H00005064-M09-img0003.jpg "Immunohistochemistry-Paraffin: Paralemmin Antibody (7C5) [H00005064-M09] - Analysis of monoclonal antibody to PALM on formalin-fixed paraffin-embedded human kidney. Antibody concentration 1.5 ug/ml")
![ELISA: Paralemmin Antibody (7C5) [H00005064-M09] - Detection limit for recombinant GST tagged PALM is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Paralemmin-Antibody-7C5-ELISA-H00005064-M09-img0002.jpg)