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![Western Blot: APP Antibody (6E10) - Chimeric [NBP2-62566] - Anti-Amyloid beta antibody 6E10. Human brain cerebral cortex (A), frontal cortex (B) and cerebellum (C) lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of 6E10 (NBP2-62566) at 0.3 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Amyloid beta is ~35kDa, though this protein is known to form SDS-resistant low-molecular weight oligomers as seen in this blot (i.e. dimers, c.f. Kumar et al. PMID: 21527912). NBP2-62566 successfully detected aggregate human Amyloid beta in human brain cerebral cortex, frontal cortex and cerebellum lysate samples.](http://images.novusbio.com/fullsize/APP-Antibody-6E10-Chimeric-Western-Blot-NBP2-62566-img0002.jpg "Western Blot: APP Antibody (6E10) - Chimeric [NBP2-62566] - Anti-Amyloid beta antibody 6E10. Human brain cerebral cortex (A), frontal cortex (B) and cerebellum (C) lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of 6E10 (NBP2-62566) at 0.3 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Amyloid beta is ~35kDa, though this protein is known to form SDS-resistant low-molecular weight oligomers as seen in this blot (i.e. dimers, c.f. Kumar et al. PMID: 21527912). NBP2-62566 successfully detected aggregate human Amyloid beta in human brain cerebral cortex, frontal cortex and cerebellum lysate samples.")
![Immunohistochemistry-Paraffin: APP Antibody (6E10) - Chimeric [NBP2-62566] - Staining of human Alzheimer's disease hippocampus tissue using anti-Amyloid Beta antibody. 6E10 Anti-Amyloid Beta staining of paraffin embedded human hippocampus affected by Alzheimer's disease using the rabbit-chimeric version of 6E10 (NBP2-62566). Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP2-62566 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Staining of amyloid plaques in the parahippocampal gyrus may be observed. Recommended concentration, 2-4 ug/ml.](http://images.novusbio.com/fullsize/APP-Antibody-6E10-Chimeric-Immunohistochemistry-Paraffin-NBP2-62566-img0001.jpg)
![Immunohistochemistry-Frozen: APP Antibody (6E10) - Chimeric [NBP2-62566] - 6E10 staining reveals amyloid plaque in frontal cortex of AD mouse model. Image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-62566_rabbit-monoclonal-app-antibody-6e10-chimeric-immunohistochemistry-frozen-284202314243..jpg)
![Immunohistochemistry-Frozen: APP Antibody (6E10) - Chimeric [NBP2-62566] - Plaque staining in AD Mouse model (Hippocampus) - without formic acid pretreatment. Image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-62566_rabbit-monoclonal-app-antibody-6e10-chimeric-immunohistochemistry-frozen-672023103423..jpg)
![Immunocytochemistry/Immunofluorescence: Supervillin Antibody (6E10) [H00006840-M03] - Analysis of monoclonal antibody to SVIL on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/Supervillin-Antibody-6E10-Immunocytochemistry-Immunofluorescence-H00006840-M03-img0002.jpg "Immunocytochemistry/Immunofluorescence: Supervillin Antibody (6E10) [H00006840-M03] - Analysis of monoclonal antibody to SVIL on HeLa cell. Antibody concentration 10 ug/ml")
![Sandwich ELISA: Supervillin Antibody (6E10) [H00006840-M03] - Detection limit for recombinant GST tagged SVIL is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Supervillin-Antibody-6E10-Sandwich-ELISA-H00006840-M03-img0003.jpg)
![Western Blot: ARMCX1 Antibody (6E10) [H00051309-M01] - Analysis of ARMCX1 expression in transfected 293T cell line by ARMCX1 monoclonal antibody (M01), clone 6E10.Lane 1: ARMCX1 transfected lysate (Predicted MW: 49.2 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/ARMCX1-Antibody-6E10-Western-Blot-H00051309-M01-img0003.jpg "Western Blot: ARMCX1 Antibody (6E10) [H00051309-M01] - Analysis of ARMCX1 expression in transfected 293T cell line by ARMCX1 monoclonal antibody (M01), clone 6E10.Lane 1: ARMCX1 transfected lysate (Predicted MW: 49.2 KDa).Lane 2: Non-transfected lysate.")
![Sandwich ELISA: ARMCX1 Antibody (6E10) [H00051309-M01] - Detection limit for recombinant GST tagged ARMCX1 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ARMCX1-Antibody-6E10-Sandwich-ELISA-H00051309-M01-img0002.jpg)
![Western Blot: CLASP1 Antibody (6E10) [H00023332-M01] - Detection against Immunogen (36.08 KDa) .](http://images.novusbio.com/fullsize/CLASP1-Antibody-6E10-Western-Blot-H00023332-M01-img0001.jpg "Western Blot: CLASP1 Antibody (6E10) [H00023332-M01] - Detection against Immunogen (36.08 KDa) .")
![Flow Cytometry: Claudin-4 Antibody (6E10) [NBP3-26654] - Overlay Peak curve showing MCF7 cells stained with Claudin-4 Antibody (6E10) (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45 min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG (H+L) at 1/200 dilution for 35 min at 4C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26654_mouse-claudin-4-mab-6e10-282202414312755.jpg "Flow Cytometry: Claudin-4 Antibody (6E10) [NBP3-26654] - Overlay Peak curve showing MCF7 cells stained with Claudin-4 Antibody (6E10) (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45 min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG (H+L) at 1/200 dilution for 35 min at 4C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.")