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![Immunoprecipitation: p97/VCP Antibody (5H12) [NBP3-26257] - Immunoprecipitating p97/VCP in U251 whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26257 in U251 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26257 (2ug) + U251 whole cell lysate (500ug). Lane 3: U251 whole cell lysate (10ug).](http://images.novusbio.com/fullsize/nbp3-26257_rabbit-p97-vcp-mab-5h12-26220241322156.jpg "Immunoprecipitation: p97/VCP Antibody (5H12) [NBP3-26257] - Immunoprecipitating p97/VCP in U251 whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26257 in U251 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26257 (2ug) + U251 whole cell lysate (500ug). Lane 3: U251 whole cell lysate (10ug).")
![Immunohistochemistry: p97/VCP Antibody (5H12) [NBP3-26257] - Image of p97/VCP Antibody (5H12) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26257_rabbit-p97-vcp-mab-5h12-26220241535573.jpg)
![Immunohistochemistry: p97/VCP Antibody (5H12) [NBP3-26257] - Image of p97/VCP Antibody (5H12) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26257_rabbit-p97-vcp-mab-5h12-27220248471918.jpg)
![Western Blot: p97/VCP Antibody (5H12) [NBP3-26257] - Positive Western Blot detected in: Hela whole cell lysate, MCF-7 whole cell lysate, U251 whole cell lysate, Rat brain tissue, Rat liver tissue. All lanes: p97/VCP Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 90 kDa Observed band size: 90 kDa](http://images.novusbio.com/fullsize/nbp3-26257_rabbit-p97-vcp-mab-5h12-28220241144267.jpg)
![Immunocytochemistry/Immunofluorescence: p97/VCP Antibody (5H12) [NBP3-26257] - Staining of SY5Y Cells with p97/VCP Antibody (5H12) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26257_rabbit-p97-vcp-mab-5h12-282202412493642.jpg)
![Western Blot: MeCP2 Antibody (5H12) [NBP2-60787] - Western blot of mouse whole brain lysate showing specificimmunolabeling of the MeCP2 protein at ~75 kDa.](http://images.novusbio.com/fullsize/MeCP2-Antibody-5H12-Western-Blot-NBP2-60787-img0001.jpg "Western Blot: MeCP2 Antibody (5H12) [NBP2-60787] - Western blot of mouse whole brain lysate showing specificimmunolabeling of the MeCP2 protein at ~75 kDa.")
![Immunocytochemistry/Immunofluorescence: MeCP2 Antibody (5H12) [NBP2-60787] - Immunofluorescence of a section of rat cerebellum labeled with anti-MeCP2 (1:500, red), colabeled with anti-GFAP (1:5000, green), and DAPI staining of nuclear DNA. The anti-GFAP labels the astrocytic cells and the processes of Bergmann glia in the molecular layer.](http://images.novusbio.com/fullsize/MeCP2-Antibody-5H12-Immunocytochemistry-Immunofluorescence-NBP2-60787-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: MeCP2 Antibody (5H12) [NBP2-60787] - Rat hippocampal section showing specific nuclear MeCP2immunolabeling in red and FOX3 in green.](http://images.novusbio.com/fullsize/MeCP2-Antibody-5H12-Immunocytochemistry-Immunofluorescence-NBP2-60787-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: MeCP2 Antibody (5H12) [NBP2-60787] - Immunofluorescence of a section of rat cerebellum showing specific labeling of MeCP2 (1:1000, red) in nuclei of neurons and specific labeling of GFAP (1:5000, green) in the network of astroglial cells and projections of Bergmann glia, and Hoechst staining of nuclear DNA.](http://images.novusbio.com/fullsize/MeCP2-Antibody-5H12-Immunocytochemistry-Immunofluorescence-NBP2-60787-img0003.jpg)
![Immunohistochemistry: SOX9 Antibody (5H12) [NBP3-26304] - Image of SOX9 Antibody (5H12) diluted at 1:100 and staining in paraffin-embedded human colon cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26304_rabbit-sox9-mab-5h12-262202414471536.jpg "Immunohistochemistry: SOX9 Antibody (5H12) [NBP3-26304] - Image of SOX9 Antibody (5H12) diluted at 1:100 and staining in paraffin-embedded human colon cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")