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![Immunocytochemistry/Immunofluorescence: PLEKHG5 Antibody (5A9) [H00057449-M01] - Analysis of monoclonal antibody to PLEKHG5 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/PLEKHG5-Antibody-5A9-Immunocytochemistry-Immunofluorescence-H00057449-M01-img0006.jpg "Immunocytochemistry/Immunofluorescence: PLEKHG5 Antibody (5A9) [H00057449-M01] - Analysis of monoclonal antibody to PLEKHG5 on HeLa cell . Antibody concentration 10 ug/ml.")
![Immunohistochemistry-Paraffin: PLEKHG5 Antibody (5A9) [H00057449-M01] - Analysis of monoclonal antibody to PLEKHG5 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 1.5 ug/ml.](http://images.novusbio.com/fullsize/PLEKHG5-Antibody-5A9-Immunohistochemistry-Paraffin-H00057449-M01-img0008.jpg)
![ELISA: PLEKHG5 Antibody (5A9) [H00057449-M01] - Detection limit for recombinant GST tagged PLEKHG5 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PLEKHG5-Antibody-5A9-ELISA-H00057449-M01-img0010.jpg)
![Western Blot: Calbindin D-28K Antibody (5A9) [NBP2-50050] - Analysis of different neuronal tissue lysates using mouse mAb to Calbindin D-28K, dilution 1:5,000: [1] protein standard, [2] rat cerebellum, [3] pig hippocampus, and [4] cow cerebellum. Bands at about 26kDa corresponds to the calbindin protein, heavily expressed in the cerebellum but a very minor component of hippocampus.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-5A9-Western-Blot-NBP2-50050-img0004.jpg "Western Blot: Calbindin D-28K Antibody (5A9) [NBP2-50050] - Analysis of different neuronal tissue lysates using mouse mAb to Calbindin D-28K, dilution 1:5,000: [1] protein standard, [2] rat cerebellum, [3] pig hippocampus, and [4] cow cerebellum. Bands at about 26kDa corresponds to the calbindin protein, heavily expressed in the cerebellum but a very minor component of hippocampus.")
![Immunocytochemistry/Immunofluorescence: Calbindin D-28K Antibody (5A9) [NBP2-50050] - Adult mouse cortex (Left) and cerebellum (Right) sections were stained with NBP2-50050 in red, and co-stained with rabbit polyclonal anti-RBFOX3/NeuN antibody (NBP1-92716, green). Calbindin is expressed in a subset of interneurons in the cortex (Left) and prominently expressed in the dendrites of Purkinje cells in the cerebellum molecular layer (Right). RBFOX3/NeuN expresses in most neurons; as a result, cells positive for Calbindin appear to be yellow. Insets are high magnification images of the boxed area. Blue is Dapi nucleus staining that labels DNA.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-5A9-Immunocytochemistry-Immunofluorescence-NBP2-50050-img0003.jpg)
![Immunohistochemistry Free-Floating: Calbindin D-28K Antibody (5A9) [NBP2-50050] - Analysis of rat brain cerebellum section stained with mouse mAb to Calbindin D-28K, dilution 1:2,000 (Red), and costained with rabbit GFAP pAb, dilution 1:5,000 (Green). DAPI staining of nuclear DNA (Blue). Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24hrs, cut to 45uM, and free-floating sections were stained with the above antibodies. The Calbindin D-28K antibody prominently labels the dendrites and perikarya of Purkinje cells in the molecular layer of the cerebellum. The GFAP antibody stains the processes of Bergmann glia in the molecular layer and astroglia in the granular and white matter layers of cerebellum.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-5A9-Immunohistochemistry-Free-Floating-NBP2-50050-img0006.jpg)
![Immunohistochemistry: Cytochrome P450 17A1 Antibody (5A9) [NBP3-26637] - Image of Cytochrome P450 17A1 Antibody (5A9) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26637_rabbit-cytochrome-p450-17a1-mab-5a9-2722024831378.jpg "Immunohistochemistry: Cytochrome P450 17A1 Antibody (5A9) [NBP3-26637] - Image of Cytochrome P450 17A1 Antibody (5A9) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: Cytochrome P450 17A1 Antibody (5A9) [NBP3-26637] - Positive Western Blot detected in: HepG2 whole cell lysate, Rat Heart whole cell lysate, Rat Brain whole cell lysate. All lanes: Cytochrome P450 17A1 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 58 kDa Observed band size: 58 kDa](http://images.novusbio.com/fullsize/nbp3-26637_rabbit-cytochrome-p450-17a1-mab-5a9-28220241140920.jpg)
![Immunocytochemistry/Immunofluorescence: Cytochrome P450 17A1 Antibody (5A9) [NBP3-26637] - Staining of Hela Cells with Cytochrome P450 17A1 Antibody (5A9) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26637_rabbit-cytochrome-p450-17a1-mab-5a9-282202412475149.jpg)
![Western Blot: SPRR2F Antibody (5A9) [H00006705-M03] - Analysis of SPRR2F expression in transfected 293T cell line by SPRR2F monoclonal antibody (M03), clone 5A9. Lane 1: SPRR2F transfected lysatE (7.8 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/SPRR2F-Antibody-5A9-Western-Blot-H00006705-M03-img0002.jpg "Western Blot: SPRR2F Antibody (5A9) [H00006705-M03] - Analysis of SPRR2F expression in transfected 293T cell line by SPRR2F monoclonal antibody (M03), clone 5A9. Lane 1: SPRR2F transfected lysatE (7.8 KDa). Lane 2: Non-transfected lysate.")
![Sandwich ELISA: SPRR2F Antibody (5A9) [H00006705-M03] - Detection limit for recombinant GST tagged SPRR2F is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SPRR2F-Antibody-5A9-Sandwich-ELISA-H00006705-M03-img0001.jpg)
![Immunoprecipitation: PI 3-Kinase p110 beta/PIK3CB Antibody (5A9) [NBP3-26383] - Immunoprecipitating PI 3-Kinase p110 beta/PIK3CB in K562 whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26383 in K562 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26383 (2ug)+ K562 whole cell lysate (500ug). Lane 3: K562 whole cell lysate (10ug).](http://images.novusbio.com/fullsize/nbp3-26383_rabbit-pi-3-kinase-p110-beta-pik3cb-mab-5a9-2622024132375.jpg "Immunoprecipitation: PI 3-Kinase p110 beta/PIK3CB Antibody (5A9) [NBP3-26383] - Immunoprecipitating PI 3-Kinase p110 beta/PIK3CB in K562 whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26383 in K562 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26383 (2ug)+ K562 whole cell lysate (500ug). Lane 3: K562 whole cell lysate (10ug).")
![Western Blot: PI 3-Kinase p110 beta/PIK3CB Antibody (5A9) [NBP3-26383] - Positive Western Blot detected in: Hela whole cell lysate, K562 whole cell lysate. All lanes: PI 3-Kinase p110 beta/PIK3CB Antibody at 1: 1500 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 123 kDa Observed band size: 110 kDa](http://images.novusbio.com/fullsize/nbp3-26383_rabbit-pi-3-kinase-p110-beta-pik3cb-mab-5a9-28220241140948.jpg)
![Western Blot: EphA4 Antibody (5A9) [H00002043-M03] - Detection against Immunogen (36.74 KDa) .](http://images.novusbio.com/fullsize/EphA4-Antibody-5A9-Western-Blot-H00002043-M03-img0001.jpg "Western Blot: EphA4 Antibody (5A9) [H00002043-M03] - Detection against Immunogen (36.74 KDa) .")
![ELISA: Angiopoietin-2 Antibody (5A9) [NBP3-26508] - The Binding Activity of Angiopoietin-2 with Angiopoietin-2 Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized Human Angiopoietin-2 at 2 ug/mL can bind Angiopoietin-2 Antibody, the EC50 is 0.6666-0.8876 ng/mL.](http://images.novusbio.com/fullsize/nbp3-26508_human-angiopoietin-2-mab-5a9-262202412272339.jpg "ELISA: Angiopoietin-2 Antibody (5A9) [NBP3-26508] - The Binding Activity of Angiopoietin-2 with Angiopoietin-2 Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized Human Angiopoietin-2 at 2 ug/mL can bind Angiopoietin-2 Antibody, the EC50 is 0.6666-0.8876 ng/mL.")