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![Western Blot: bcl-x Antibody (2H12) [NBP2-32917] - Rat and mouse neonatal cardiomyocytes. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Bcl-xL-Antibody-2H12-Western-Blot-NBP2-32917-img0004.jpg "Western Blot: bcl-x Antibody (2H12) [NBP2-32917] - Rat and mouse neonatal cardiomyocytes. Western blot image submitted by a verified customer review.")
![Immunohistochemistry-Paraffin: bcl-x Antibody (2H12) [NBP2-32917] - FFPE human Hodgkin's lymphoma stained with bcl-x Ab (2H12). Note cytoplasmic and membrane staining.](http://images.novusbio.com/fullsize/Bcl-xL-Antibody-2H12-Immunohistochemistry-Paraffin-NBP2-32917-img0001.jpg)
![Western Blot: bcl-x Antibody (2H12) [NBP2-32917] - Western blot analysis of Jurkat Cell lysate using bcl-X Ab (2H12).](http://images.novusbio.com/fullsize/Bcl-xL-Antibody-2H12-Western-Blot-NBP2-32917-img0003.jpg)
![Immunohistochemistry: RNF20 Antibody (2H12) [NBP3-26341] - Image of RNF20 Antibody (2H12) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26341_rabbit-rnf20-mab-2h12-262202414225224.jpg "Immunohistochemistry: RNF20 Antibody (2H12) [NBP3-26341] - Image of RNF20 Antibody (2H12) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: RNF20 Antibody (2H12) [NBP3-26341] - Image of RNF20 Antibody (2H12) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26341_rabbit-rnf20-mab-2h12-26220241525337.jpg)
![Western Blot: RNF20 Antibody (2H12) [NBP3-26341] - Positive Western Blot detected in: Hela whole cell lysate, 293 whole cell lysate. All lanes: RNF20 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 114 kDa Observed band size: 114 kDa](http://images.novusbio.com/fullsize/nbp3-26341_rabbit-rnf20-mab-2h12-282202411472147.jpg)
![Immunocytochemistry/Immunofluorescence: RNF20 Antibody (2H12) [NBP3-26341] - Staining of Hela Cells with RNF20 Antibody (2H12) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26341_rabbit-rnf20-mab-2h12-282202412475144.jpg)
![Immunoprecipitation: HDAC6 Antibody (2H12) [NBP3-26236] - Immunoprecipitating HDAC6 in HepG2 whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26236 in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26236 (3ug) + HepG2 whole cell lysate (500ug). Lane 3: HepG2 whole cell lysate (20ug).](http://images.novusbio.com/fullsize/nbp3-26236_rabbit-hdac6-mab-2h12-26220241322159.jpg "Immunoprecipitation: HDAC6 Antibody (2H12) [NBP3-26236] - Immunoprecipitating HDAC6 in HepG2 whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26236 in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26236 (3ug) + HepG2 whole cell lysate (500ug). Lane 3: HepG2 whole cell lysate (20ug).")
![Immunohistochemistry: HDAC6 Antibody (2H12) [NBP3-26236] - Image of HDAC6 Antibody (2H12) diluted at 1:112.5 and staining in paraffin-embedded human liver cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26236_rabbit-hdac6-mab-2h12-262202414471573.jpg)
![Western Blot: HDAC6 Antibody (2H12) [NBP3-26236] - Positive Western Blot detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate. All lanes: HDAC6 Antibody at 1.1ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 132, 115 KDa Observed band size: 160 KDa](http://images.novusbio.com/fullsize/nbp3-26236_rabbit-hdac6-mab-2h12-282202411413619.jpg)
![Western Blot: bcl-x Antibody (2H12) [NBP3-11881] - Western blot analysis of thymocytes from human, mouse and rat. Cell lysates from approximately 106 cells were loaded into each lane of a 12% SDS-PAGE gel and blotted onto PVDF membranes. Exalpha's anti-Bcl-XL antibody was used at 1 ug/ml - detection was by chemiluminescence detection using an anti-mouse secondary antibody.](http://images.novusbio.com/fullsize/bcl-x-Antibody-2H12-Western-Blot-NBP3-11881-img0001.jpg "Western Blot: bcl-x Antibody (2H12) [NBP3-11881] - Western blot analysis of thymocytes from human, mouse and rat. Cell lysates from approximately 106 cells were loaded into each lane of a 12% SDS-PAGE gel and blotted onto PVDF membranes. Exalpha's anti-Bcl-XL antibody was used at 1 ug/ml - detection was by chemiluminescence detection using an anti-mouse secondary antibody.")
![Immunohistochemistry: Neutrophil Elastase/ELA2 Antibody (2H12) [NBP3-26603] - Image of Neutrophil Elastase/ELA2 Antibody (2H12) diluted at 1:100 and staining in paraffin-embedded human spleen tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26603_rabbit-neutrophil-elastase-ela2-mab-2h12-272202485634.jpg "Immunohistochemistry: Neutrophil Elastase/ELA2 Antibody (2H12) [NBP3-26603] - Image of Neutrophil Elastase/ELA2 Antibody (2H12) diluted at 1:100 and staining in paraffin-embedded human spleen tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Flow Cytometry: Neutrophil Elastase/ELA2 Antibody (2H12) [NBP3-26603] - Overlay histogram showing Jurkat cells stained with Neutrophil Elastase/ELA2 Antibody (2H12) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26603_rabbit-neutrophil-elastase-ela2-mab-2h12-282202414183686.jpg)