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![Western Blot: Citrulline Antibody (2D3.1) [NBP2-59364] - Western Blot analysis of Citrulline-BSA Conjugate showing detection of 67 kDa Citrulline protein using Mouse Anti-Citrulline Monoclonal Antibody, Clone 2D3-1B9 (NBP2-59364). Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 ug). Lane 3: BSA (2.0 ug). Lane 4: Citrulline-BSA (0.5 ug). Lane 5: Citrulline-BSA (2.0 ug). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Citrulline Monoclonal Antibody (NBP2-59364) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.](http://images.novusbio.com/fullsize/Citrulline-Antibody-2D3-1-Western-Blot-NBP2-59364-img0005.jpg "Western Blot: Citrulline Antibody (2D3.1) [NBP2-59364] - Western Blot analysis of Citrulline-BSA Conjugate showing detection of 67 kDa Citrulline protein using Mouse Anti-Citrulline Monoclonal Antibody, Clone 2D3-1B9 (NBP2-59364). Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 ug). Lane 3: BSA (2.0 ug). Lane 4: Citrulline-BSA (0.5 ug). Lane 5: Citrulline-BSA (2.0 ug). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Citrulline Monoclonal Antibody (NBP2-59364) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.")
![Immunocytochemistry/Immunofluorescence: Citrulline Antibody (2D3.1) [NBP2-59364] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Citrulline Monoclonal Antibody, Clone 2D3.1 (NBP2-59364). Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Citrulline Monoclonal Antibody (NBP2-59364) at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alexa Fluor 633 F-Actin stain. (C) Citrulline Antibody (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.](http://images.novusbio.com/fullsize/Citrulline-Antibody-2D3-1-Immunocytochemistry-Immunofluorescence-NBP2-59364-img0007.jpg)
![Immunohistochemistry: Citrulline Antibody (2D3.1) [NBP2-59364] - Immunohistochemistry analysis using Mouse Anti-Citrulline Monoclonal Antibody, Clone 2D3.1 (NBP2-59364). Tissue: Thyroid Cancer. Species: Human. Primary Antibody: Mouse Anti-Citrulline Monoclonal Antibody (NBP2-59364) at 1:100 for Overnight at 4C, then 30 min at 37C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37C. Counterstain: DAPI for 3 min at RT. Magnification: 5X.](http://images.novusbio.com/fullsize/Citrulline-Antibody-2D3-1-Immunohistochemistry-NBP2-59364-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Citrulline Antibody (2D3.1) [NBP2-59364] - Tissue: Embryonic kidney cells (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Citrulline Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom).Courtesy of: Dr. Robert Burke, University of Victoria.](http://images.novusbio.com/fullsize/Citrulline-Antibody-2D3-1-Immunocytochemistry-Immunofluorescence-NBP2-59364-img0002.jpg)
![Dot Blot: Crotonaldehyde Antibody (2D3.1) [NBP2-61922] - Dot blot analysis using Mouse Anti-Crotonaldehyde Monoclonal Antibody, Clone 2D3.1 (NBP2-61922). Primary Antibody: Mouse Anti-Crotonaldehyde Monoclonal Antibody (NBP2-61922) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:3000 for 1 hour at RT. The quantities of protein spotted on each panel are as shown.](http://images.novusbio.com/fullsize/Crotonaldehyde-Antibody-2D3-1-Dot-Blot-NBP2-61922-img0002.jpg "Dot Blot: Crotonaldehyde Antibody (2D3.1) [NBP2-61922] - Dot blot analysis using Mouse Anti-Crotonaldehyde Monoclonal Antibody, Clone 2D3.1 (NBP2-61922). Primary Antibody: Mouse Anti-Crotonaldehyde Monoclonal Antibody (NBP2-61922) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:3000 for 1 hour at RT. The quantities of protein spotted on each panel are as shown.")