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![Immunohistochemistry: Ferritin Light Chain Antibody (12D12) [NBP3-26566] - Image of Ferritin Light Chain Antibody (12D12) diluted at 1:50 and staining in paraffin-embedded human glioma cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.29% DAB.](http://images.novusbio.com/fullsize/nbp3-26566_rabbit-ferritin-light-chain-mab-12d12-2622024151069.jpg "Immunohistochemistry: Ferritin Light Chain Antibody (12D12) [NBP3-26566] - Image of Ferritin Light Chain Antibody (12D12) diluted at 1:50 and staining in paraffin-embedded human glioma cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.29% DAB.")
![Flow Cytometry: Ferritin Light Chain Antibody (12D12) [NBP3-26566] - Overlay Peak curve showing MCF-7 cells stained with Ferritin Light Chain Antibody (12D12) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26566_rabbit-ferritin-light-chain-mab-12d12-28220241431274.jpg)
![ELISA: VSIG4 Antibody (12D12) [NBP3-26254] - The Binding Activity of VSIG4 with VSIG4 Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized Human VSIG4 at 2 ug/ml can bind VSIG4 Antibody, the EC50 is 51.14-68.73 ng/mL.](http://images.novusbio.com/fullsize/nbp3-26254_human-vsig4-mab-12d12-262202412272312.jpg "ELISA: VSIG4 Antibody (12D12) [NBP3-26254] - The Binding Activity of VSIG4 with VSIG4 Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized Human VSIG4 at 2 ug/ml can bind VSIG4 Antibody, the EC50 is 51.14-68.73 ng/mL.")
![Flow Cytometry: CD39/ENTPD1 Antibody (12D12) [NBP3-26601] - Overlay Peak curve showing PC3 cells stained with CD39/ENTPD1 Antibody (12D12) (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26601_rabbit-cd39-entpd1-mab-12d12-282202414183614.jpg "Flow Cytometry: CD39/ENTPD1 Antibody (12D12) [NBP3-26601] - Overlay Peak curve showing PC3 cells stained with CD39/ENTPD1 Antibody (12D12) (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.")