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![Western Blot: HSF1 Antibody (10H4) [NBP2-42205] - Western Blot analysis of Human A431 and HEK293 cell lysates showing detection of HSF1 protein using Rat Anti-HSF1 Monoclonal Antibody, Clone 10H4 (NBP2-42205). Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody (NBP2-42205) at 1:1000.](http://images.novusbio.com/fullsize/HSF1-Antibody-10H4-Western-Blot-NBP2-42205-img0004.jpg "Western Blot: HSF1 Antibody (10H4) [NBP2-42205] - Western Blot analysis of Human A431 and HEK293 cell lysates showing detection of HSF1 protein using Rat Anti-HSF1 Monoclonal Antibody, Clone 10H4 (NBP2-42205). Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody (NBP2-42205) at 1:1000.")
![Immunocytochemistry/Immunofluorescence: HSF1 Antibody (10H4) [NBP2-42205] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: R-PE Goat Anti-Rat (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Localizes to the nucleus upon activation. Magnification: 100x. Heat Shocked at 42C for 1h.](http://images.novusbio.com/fullsize/HSF1-Antibody-10H4-Immunocytochemistry-Immunofluorescence-NBP2-42205-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: HSF1 Antibody (10H4) [NBP2-42205] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Rat (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Localizes to the nucleus upon activation. Magnification: 20x. Heat Shocked at 42C for 1h.](http://images.novusbio.com/fullsize/HSF1-Antibody-10H4-Immunocytochemistry-Immunofluorescence-NBP2-42205-img0003.jpg)
![Western Blot: KNTC1 Antibody (10H4) [H00009735-M01] - KNTC1 monoclonal antibody (M01), clone 10H4 Analysis of KNTC1 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/KNTC1-Antibody-10H4-Western-Blot-H00009735-M01-img0007.jpg "Western Blot: KNTC1 Antibody (10H4) [H00009735-M01] - KNTC1 monoclonal antibody (M01), clone 10H4 Analysis of KNTC1 expression in Hela S3 NE.")
![Immunocytochemistry/Immunofluorescence: KNTC1 Antibody (10H4) [H00009735-M01] - Analysis of monoclonal antibody to KNTC1 on HeLa cell. Antibody concentration 50 ug/ml.](http://images.novusbio.com/fullsize/KNTC1-Antibody-10H4-Immunocytochemistry-Immunofluorescence-H00009735-M01-img0005.jpg)
![Sandwich ELISA: KNTC1 Antibody (10H4) [H00009735-M01] - Detection limit for recombinant GST tagged KNTC1 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/KNTC1-Antibody-10H4-Sandwich-ELISA-H00009735-M01-img0006.jpg)
![Western Blot: IRAK4 Antibody (10H4) [NBP3-15153] - Positive WB detected in: THP-1 whole cell lysate, U251 whole cell lysate. All lanes: IRAK4 antibody at 1:2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 52, 38 KDa. Observed band size: 55 KDa.](http://images.novusbio.com/fullsize/IRAK4-Antibody-10H4-Western-Blot-NBP3-15153-img0002.jpg "Western Blot: IRAK4 Antibody (10H4) [NBP3-15153] - Positive WB detected in: THP-1 whole cell lysate, U251 whole cell lysate. All lanes: IRAK4 antibody at 1:2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 52, 38 KDa. Observed band size: 55 KDa.")
![Flow Cytometry: IRAK4 Antibody (10H4) [NBP3-15153] - Overlay histogram showing Jurkat cells stained with IRAK4 antibody (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1ug/1*106cells) for 1 h at 4C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/IRAK4-Antibody-10H4-Flow-Cytometry-NBP3-15153-img0001.jpg)