Hu,
Mu,
Rt,
Av,
Ch,
In,
Ma,
Pl,
Pm,
YeSpecies Glossary
Reactivity: Hu, Mu, Rt, Av, Ch, In, Ma, Pl, Pm, Ye
WB, ELISA, Flow, ICC/IF, IHC, IP, MS
Applications: WB, ELISA, Flow, ICC/IF, IHC, IP, MS
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
![Western Blot: KDEL Antibody (10C3) [NBP1-97469] - Lane 1: Grp78 (BiP) recombinant protein, Lane 2: RK-13, Lane 3: Mouse liver microsomes, Lane 4: Rat liver microsomes, Lane 5: HeLa Cell Lysate (heat shocked).](http://images.novusbio.com/fullsize/KDEL-Antibody-10C3-Western-Blot-NBP1-97469-img0004.jpg "Western Blot: KDEL Antibody (10C3) [NBP1-97469] - Lane 1: Grp78 (BiP) recombinant protein, Lane 2: RK-13, Lane 3: Mouse liver microsomes, Lane 4: Rat liver microsomes, Lane 5: HeLa Cell Lysate (heat shocked).")
![Immunocytochemistry/Immunofluorescence: KDEL Antibody (10C3) [NBP1-97469] - KDEL in human primary fibroblasts. Antibody at 1:600 in PBST, 0.1% triton + 10% DS. O/N incubation at 4C. Donkey anti-mouse Alexa Fluor 488 at 1:500 in PBST, 0.1% triton + 10% DS 1h RT. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/KDEL-Antibody-10C3-Immunocytochemistry-Immunofluorescence-NBP1-97469-img0006.jpg)
![Immunohistochemistry-Paraffin: KDEL Antibody (10C3) [NBP1-97469] - Analysis of human spleen tissue stained with KDEL, mAb (10C3) at 10 ug/mL.](http://images.novusbio.com/fullsize/KDEL-Antibody-10C3-Immunohistochemistry-Paraffin-NBP1-97469-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: KDEL Antibody (10C3) [NBP1-97469] - Analysis of endoplasmic reticulum staining of mouse C2C12 myoblasts transfected with wild type mouse ADAM12.](http://images.novusbio.com/fullsize/KDEL-Antibody-10C3-Immunocytochemistry-Immunofluorescence-NBP1-97469-img0005.jpg)
![Immunohistochemistry: Serpin E1/PAI-1 Antibody (10C3) [NBP3-26327] - Image of Serpin E1/PAI-1 Antibody (10C3) diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26327_rabbit-serpin-e1-pai-1-mab-10c3-2722024827491.jpg "Immunohistochemistry: Serpin E1/PAI-1 Antibody (10C3) [NBP3-26327] - Image of Serpin E1/PAI-1 Antibody (10C3) diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: Serpin E1/PAI-1 Antibody (10C3) [NBP3-26327] - Positive Western Blot detected in: U-251 whole cell lysate, U-87 whole cell lysate. All lanes: PAI1 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 46, 44 kDa Observed band size: 45 kDa](http://images.novusbio.com/fullsize/nbp3-26327_rabbit-serpin-e1-pai-1-mab-10c3-28220241140947.jpg)
![Western Blot: CDC37 Antibody (10C3) [NBP3-26671] - Positive Western Blot detected in: Hela whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, PC-3 whole cell lysate. All lanes: CDC37 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 45 kDa Observed band size: 50 kDa](http://images.novusbio.com/fullsize/nbp3-26671_rabbit-cdc37-mab-10c3-28220241152525.jpg "Western Blot: CDC37 Antibody (10C3) [NBP3-26671] - Positive Western Blot detected in: Hela whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, PC-3 whole cell lysate. All lanes: CDC37 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 45 kDa Observed band size: 50 kDa")
![Flow Cytometry: CDC37 Antibody (10C3) [NBP3-26671] - Overlay histogram showing Hela cells stained with CDC37 Antibody (10C3) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26671_rabbit-cdc37-mab-10c3-282202414312749.jpg)