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![Western Blot: Max Antibody (103) [NBP2-90153] - Lane A: Jurkat Whole Cell LysateLane B: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:18 kDaObserved band size:20 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/Max-Antibody-103-Western-Blot-NBP2-90153-img0004.jpg "Western Blot: Max Antibody (103) [NBP2-90153] - Lane A: Jurkat Whole Cell LysateLane B: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:18 kDaObserved band size:20 kDa(We are unsure as to the identity of these extra bands.)")
![Immunocytochemistry/Immunofluorescence: Max Antibody (103) [NBP2-90153] - Staining of Human MAX in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human MAX monoclonal antibody (1:300) at 37? 1 hour. Then cells were stained with the Alexa Fluor® 594-conjugated goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to nucleus.](http://images.novusbio.com/fullsize/Max-Antibody-103-Immunocytochemistry-Immunofluorescence-NBP2-90153-img0001.jpg)
![Flow Cytometry: Max Antibody (103) [NBP2-90153] - Analysis of Human MAX expression on Raji cells stained with purified anti-Human MAX, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/Max-Antibody-103-Flow-Cytometry-NBP2-90153-img0002.jpg)
![Immunoprecipitation: Max Antibody (103) [NBP2-90153] - Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg 293T Whole Cell Lysate0.5 uL anti-MAX rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-MAX rabbit monoclonal antibody,at 1:1000 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 23 kDaObserved band size: 23 kDa](http://images.novusbio.com/fullsize/Max-Antibody-103-Immunoprecipitation-NBP2-90153-img0003.jpg)
![Immunohistochemistry-Paraffin: VAP-B Antibody (103) [NBP2-89625] - Staining of human VAPB in human brain with rabbit monoclonal antibody (1:300).](http://images.novusbio.com/fullsize/VAP-B-Antibody-103-Immunohistochemistry-Paraffin-NBP2-89625-img0004.jpg "Immunohistochemistry-Paraffin: VAP-B Antibody (103) [NBP2-89625] - Staining of human VAPB in human brain with rabbit monoclonal antibody (1:300).")
![Immunohistochemistry-Paraffin: VAP-B Antibody (103) [NBP2-89625] - Staining of human VAPB in human pancreas with rabbit monoclonal antibody (1:300).](http://images.novusbio.com/fullsize/VAP-B-Antibody-103-Immunohistochemistry-Paraffin-NBP2-89625-img0001.jpg)
![Immunohistochemistry-Paraffin: VAP-B Antibody (103) [NBP2-89625] - Staining of human VAPB in human liver with rabbit monoclonal antibody (1:300)](http://images.novusbio.com/fullsize/VAP-B-Antibody-103-Immunohistochemistry-Paraffin-NBP2-89625-img0002.jpg)
![Immunohistochemistry-Paraffin: VAP-B Antibody (103) [NBP2-89625] - Staining of human VAPB in human cirrhosis with rabbit monoclonal antibody (1:300).](http://images.novusbio.com/fullsize/VAP-B-Antibody-103-Immunohistochemistry-Paraffin-NBP2-89625-img0003.jpg)
![Western Blot: RhoA Antibody (103) [NBP2-90107] - Anti-RHOA rabbit monoclonal antibody at 1:500 dilution. Lane A: MCF7 Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:22 kDa. Observed band size:22 kDa.](http://images.novusbio.com/fullsize/RhoA-Antibody-103-Western-Blot-NBP2-90107-img0003.jpg "Western Blot: RhoA Antibody (103) [NBP2-90107] - Anti-RHOA rabbit monoclonal antibody at 1:500 dilution. Lane A: MCF7 Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:22 kDa. Observed band size:22 kDa.")
![Immunocytochemistry/Immunofluorescence: RhoA Antibody (103) [NBP2-90107] - Immunofluorescence staining of RHOA in Hela cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human RHOA monoclonal antibody (dilution ratio 1:60) at 4? overnight. Then cells were stained with the Alexa Fluor-488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/RhoA-Antibody-103-Immunocytochemistry-Immunofluorescence-NBP2-90107-img0002.jpg)
![Western Blot: Purine Nucleoside Phosphorylase/PNP Antibody (103) [NBP2-90053] - Lane A: Jurkat Whole Cell LysateLane B: K562 Whole Cell LysateLane C: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:32 kDaObserved band size:32 kDa](http://images.novusbio.com/fullsize/Purine-Nucleoside-Phosphorylase-PNP-Antibody-103-Western-Blot-NBP2-90053-img0002.jpg "Western Blot: Purine Nucleoside Phosphorylase/PNP Antibody (103) [NBP2-90053] - Lane A: Jurkat Whole Cell LysateLane B: K562 Whole Cell LysateLane C: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:32 kDaObserved band size:32 kDa")
![Immunoprecipitation: Purine Nucleoside Phosphorylase/PNP Antibody (103) [NBP2-90053] - Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg K562 Whole Cell Lysate2 uL anti-PNP-3 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-PNP-3 rabbit monoclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 33 kDaObserved band size: 33 kDa](http://images.novusbio.com/fullsize/Purine-Nucleoside-Phosphorylase-PNP-Antibody-103-Immunoprecipitation-NBP2-90053-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CEACAM1/CD66a Antibody (103) [NBP2-89666] - Staining of CEACAM1 in HT29 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human CEACAM1 monoclonal antibody (1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green).](http://images.novusbio.com/fullsize/CEACAM1-CD66a-Antibody-103-Immunocytochemistry-Immunofluorescence-NBP2-89666-img0002.jpg "Immunocytochemistry/Immunofluorescence: CEACAM1/CD66a Antibody (103) [NBP2-89666] - Staining of CEACAM1 in HT29 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human CEACAM1 monoclonal antibody (1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green).")
![Flow Cytometry: CEACAM1/CD66a Antibody (103) [NBP2-89666] - Analysis of Human CEACAM1(CD66a) expression in HT29 cells. The cells were harvested and stained with purified Rabbit anti-human CEACAM1, then a FITC-conjugated second step antibody. Flow cytometry was performed on a BD (TM) FACSCalibur flow cytometry system.](http://images.novusbio.com/fullsize/CEACAM1-CD66a-Antibody-103-Flow-Cytometry-NBP2-89666-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: B7-2/CD86 Antibody (103) [NBP2-89607] - Staining of B7-2 in Raji cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human B7-2 monoclonal antibody (dilution ratio 1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to .](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-103-Immunocytochemistry-Immunofluorescence-NBP2-89607-img0001.jpg "Immunocytochemistry/Immunofluorescence: B7-2/CD86 Antibody (103) [NBP2-89607] - Staining of B7-2 in Raji cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human B7-2 monoclonal antibody (dilution ratio 1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to .")