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![Immunocytochemistry/Immunofluorescence: L1CAM Antibody (014) [NBP2-89294] - Staining of Human CD171 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-Human CD171 monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor (R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cells membrane.](http://images.novusbio.com/fullsize/L1CAM-Antibody-014-Immunocytochemistry-Immunofluorescence-NBP2-89294-img0003.jpg "Immunocytochemistry/Immunofluorescence: L1CAM Antibody (014) [NBP2-89294] - Staining of Human CD171 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-Human CD171 monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor (R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cells membrane.")
![Immunohistochemistry-Paraffin: L1CAM Antibody (014) [NBP2-89294] - Staining of human CD171 in human appendix with rabbit monoclonal antibody (1:200). Positive staining was localized to myenteric nerve plexus.](http://images.novusbio.com/fullsize/L1CAM-Antibody-014-Immunohistochemistry-Paraffin-NBP2-89294-img0001.jpg)
![Flow Cytometry: L1CAM Antibody (014) [NBP2-89294] - Analysis of human CD171 expression on HeLa cells. Cells were stained with purified anti-Human CD171, then a FITC-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/L1CAM-Antibody-014-Flow-Cytometry-NBP2-89294-img0002.jpg)
![Western Blot: PMVK/phosphomevalonate kinase Antibody (014) [NBP2-90297] - Lane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:21 kDaObserved band size:24 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/PMVK-phosphomevalonate-kinase-Antibody-014-Western-Blot-NBP2-90297-img0001.jpg "Western Blot: PMVK/phosphomevalonate kinase Antibody (014) [NBP2-90297] - Lane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:21 kDaObserved band size:24 kDa(We are unsure as to the identity of these extra bands.)")
![Immunoprecipitation: PMVK/phosphomevalonate kinase Antibody (014) [NBP2-90297] - Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg NIH-3T3 Whole Cell Lysate0.5 uL anti-PMVK rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-PMVK rabbit monoclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 21 kDaObserved band size: 21 kDa](http://images.novusbio.com/fullsize/PMVK-phosphomevalonate-kinase-Antibody-014-Immunoprecipitation-NBP2-90297-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: 4-1BB Ligand/TNFSF9 Antibody (014) [NBP2-90388] - Confocal immunofluorescence analysis of Mouse TNFSF9 in RAW264.7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Mouse TNFSF9 monoclonal antibody (1:100) at 4? overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.](http://images.novusbio.com/fullsize/4-1BB-Ligand-TNFSF9-Antibody-014-Immunocytochemistry-Immunofluorescence-NBP2-90388-img0001.jpg "Immunocytochemistry/Immunofluorescence: 4-1BB Ligand/TNFSF9 Antibody (014) [NBP2-90388] - Confocal immunofluorescence analysis of Mouse TNFSF9 in RAW264.7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Mouse TNFSF9 monoclonal antibody (1:100) at 4? overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.")
![Flow Cytometry: 4-1BB Ligand/TNFSF9 Antibody (014) [NBP2-90388] - Analysis of Mouse TNFSF9 expression on Raw264.7 cells. Cells were stained with purified anti-Mouse TNFSF9, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/4-1BB-Ligand-TNFSF9-Antibody-014-Flow-Cytometry-NBP2-90388-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: TREM2 Antibody (014) [NBP2-90452] - Immunofluorescence staining of TREM2 in RAW264.7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-mouse TREM2 monoclonal antibody (dilution ratio 1:60) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm and cell membrane.](http://images.novusbio.com/fullsize/TREM2-Antibody-014-Immunocytochemistry-Immunofluorescence-NBP2-90452-img0001.jpg "Immunocytochemistry/Immunofluorescence: TREM2 Antibody (014) [NBP2-90452] - Immunofluorescence staining of TREM2 in RAW264.7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-mouse TREM2 monoclonal antibody (dilution ratio 1:60) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm and cell membrane.")
![Western Blot: Fibronectin Antibody (014) [NBP2-89407] - Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:233 kDa Observed band size:250 kDa](http://images.novusbio.com/fullsize/Fibronectin-Antibody-014-Western-Blot-NBP2-89407-img0001.jpg "Western Blot: Fibronectin Antibody (014) [NBP2-89407] - Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:233 kDa Observed band size:250 kDa")
![Immunoprecipitation: Fibronectin Antibody (014) [NBP2-89407] - Lane A:0.5 mg HepG2 Whole Cell Lysate 2 uL anti-FF2 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-FF2 rabbit monoclonal antibody,at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 262 kDa Observed band size: 262 kDa](http://images.novusbio.com/fullsize/Fibronectin-Antibody-014-Immunoprecipitation-NBP2-89407-img0002.jpg)
![Immunoprecipitation: AK3L1 Antibody (014) [NBP2-90094] - Lane A:0.5 mg MCF-7 Whole Cell LysateLane B:0.5 mg HepG2 Whole Cell LysateLane C:0.5 mg 293T Whole Cell LysateLane D:0.5 mg A431 Whole Cell Lysate2 uL anti-AK4 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-AK4 rabbit monoclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 26 kDaObserved band size: 26 kDa](http://images.novusbio.com/fullsize/AK3L1-Antibody-014-Immunoprecipitation-NBP2-90094-img0001.jpg "Immunoprecipitation: AK3L1 Antibody (014) [NBP2-90094] - Lane A:0.5 mg MCF-7 Whole Cell LysateLane B:0.5 mg HepG2 Whole Cell LysateLane C:0.5 mg 293T Whole Cell LysateLane D:0.5 mg A431 Whole Cell Lysate2 uL anti-AK4 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-AK4 rabbit monoclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 26 kDaObserved band size: 26 kDa")