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![Western Blot: STAT1 Antibody (OTI4H9) [NBP1-48011] - Analysis of extracts (10ug) from 7 Human tissue by using anti-STAT1 monoclonal antibody ( 1: Uterus; 2: Breast; 3: Brain; 4: Liver; 5: Ovary; 6: Thyroid gland; 7: colon) at 1:200 dilution.](http://images.novusbio.com/fullsize/STAT1-Antibody-OTI4H9-Western-Blot-NBP1-48011-img0009.jpg "Western Blot: STAT1 Antibody (OTI4H9) [NBP1-48011] - Analysis of extracts (10ug) from 7 Human tissue by using anti-STAT1 monoclonal antibody ( 1: Uterus; 2: Breast; 3: Brain; 4: Liver; 5: Ovary; 6: Thyroid gland; 7: colon) at 1:200 dilution.")
![Immunocytochemistry/Immunofluorescence: STAT1 Antibody (4H9) [NBP1-48011] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY STAT1.](http://images.novusbio.com/fullsize/STAT1-Antibody-4H9-Immunocytochemistry-Immunofluorescence-NBP1-48011-img0007.jpg)
![Immunohistochemistry-Paraffin: STAT1 Antibody (4H9) [NBP1-48011] - Staining of paraffin-embedded Human Kidney tissue using anti-STAT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/STAT1-Antibody-4H9-Immunohistochemistry-Paraffin-NBP1-48011-img0006.jpg)
![Western Blot: STAT1 Antibody (4H9) [NBP1-48011] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY STAT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STAT1.](http://images.novusbio.com/fullsize/STAT1-Antibody-4H9-Western-Blot-NBP1-48011-img0001.jpg)
![Western Blot: STAT1 Antibody (4H9) [NBP1-48011] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-STAT1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/STAT1-Antibody-4H9-Western-Blot-NBP1-48011-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: STAT1 Antibody (4H9) [NBP1-48011] - Immunofluorescent staining of HT29 cells using anti-STAT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/STAT1-Antibody-4H9-Immunocytochemistry-Immunofluorescence-NBP1-48011-img0002.jpg)
![Immunohistochemistry-Paraffin: STAT1 Antibody (4H9) [NBP1-48011] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-STAT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/STAT1-Antibody-4H9-Immunohistochemistry-Paraffin-NBP1-48011-img0003.jpg)
![Immunohistochemistry-Paraffin: STAT1 Antibody (4H9) [NBP1-48011] - Staining of paraffin-embedded Human breast tissue using anti-STAT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/STAT1-Antibody-4H9-Immunohistochemistry-Paraffin-NBP1-48011-img0004.jpg)
![Immunohistochemistry-Paraffin: STAT1 Antibody (4H9) [NBP1-48011] - Staining of paraffin-embedded Human endometrium tissue using anti-STAT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/STAT1-Antibody-4H9-Immunohistochemistry-Paraffin-NBP1-48011-img0005.jpg)
![Western Blot: STAT1 Antibody [NB100-56314] - Western blot analysis of STAT1 in HeLa cell lysate using NB100-56314.](http://images.novusbio.com/fullsize/STAT1-Antibody-Western-Blot-NB100-56314-img0002.jpg "Western Blot: STAT1 Antibody [NB100-56314] - Western blot analysis of STAT1 in HeLa cell lysate using NB100-56314.")
![Immunocytochemistry/Immunofluorescence: STAT1 Antibody [NB100-56314] - RH30 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-STAT1 Antibody NB100-56314 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/STAT1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56314-img0004.jpg)
![Immunohistochemistry-Paraffin: STAT1 Antibody [NB100-56314] - Analysis of a FFPE tissue section of human lymph node using 1:200 dilution of STAT1 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. Strong nuclear staining in germinal center and surrounding cells along with cytoplasmic reactivity was observed.](http://images.novusbio.com/fullsize/STAT1-Antibody-Immunohistochemistry-Paraffin-NB100-56314-img0003.jpg)
![Western Blot: STAT1 Antibody [NBP1-81578] - Analysis in human cell lines SK-MEL-30 and HEK293. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.](http://images.novusbio.com/fullsize/STAT1-Antibody-Western-Blot-NBP1-81578-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: STAT1 Antibody [NBP1-81578] - Analysis in human cell lines SK-MEL-30 and HEK293. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: STAT1 Antibody [NBP1-81578] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/STAT1-Antibody-Western-Blot-NBP1-81578-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: STAT1 Antibody [NBP1-81578] - Staining of human cell line A-431 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/STAT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81578-img0008.jpg)
![Immunohistochemistry-Paraffin: STAT1 Antibody [NBP1-81578] - Staining of human skeletal muscle shows no positivity in a subset of myocytes as expected.](http://images.novusbio.com/fullsize/STAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-81578-img0017.jpg)
![Immunohistochemistry-Paraffin: STAT1 Antibody [NBP1-81578] - Staining of human lymph node shows cytoplasmic positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/STAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-81578-img0010.jpg)
![Immunohistochemistry-Paraffin: STAT1 Antibody [NBP1-81578] - Staining of human kidney shows weak cytoplasmic positivity in cells in distal tubules.](http://images.novusbio.com/fullsize/STAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-81578-img0015.jpg)
![Immunohistochemistry-Paraffin: STAT1 Antibody [NBP1-81578] - Staining of human rectum shows moderate cytoplasmic positivity in lymphoid cells.](http://images.novusbio.com/fullsize/STAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-81578-img0016.jpg)
![Immunohistochemistry-Paraffin: STAT1 Antibody [NBP1-81579] - Analysis in human urinary bladder and skeletal muscle tissues using NBP1-81579 antibody. Corresponding STAT1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/STAT1-Antibody-NBP1-81579-img0019.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: STAT1 Antibody [NBP1-81579] - Analysis in human urinary bladder and skeletal muscle tissues using NBP1-81579 antibody. Corresponding STAT1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: STAT1 Antibody [NBP1-81579] - Analysis in human cell lines SK-MEL-30 and HEK293 using Anti-STAT1 antibody. Corresponding STAT1 RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.](http://images.novusbio.com/fullsize/STAT1-Antibody-Western-Blot-NBP1-81579-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: STAT1 Antibody [NBP1-81579] - Staining of human cell line A-431 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/STAT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81579-img0013.jpg)
![Immunohistochemistry-Paraffin: STAT1 Antibody [NBP1-81579] - Staining of human skeletal muscle shows very weak positivity in myocytes.](http://images.novusbio.com/fullsize/STAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-81579-img0022.jpg)
![Immunohistochemistry-Paraffin: STAT1 Antibody [NBP1-81579] - Staining of human lymph node shows cytoplasmic positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/STAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-81579-img0017.jpg)
![Immunohistochemistry-Paraffin: STAT1 Antibody [NBP1-81579] - Staining of human urinary bladder shows strong cytoplasmic and membranous positivity in urotheilal cells.](http://images.novusbio.com/fullsize/STAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-81579-img0020.jpg)
![Immunohistochemistry-Paraffin: STAT1 Antibody [NBP1-81579] - Staining of human prostate shows moderate cytoplasmic and membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/STAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-81579-img0021.jpg)
![Western Blot: STAT1 Antibody (15H3) [NBP1-48013] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY STAT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STAT1.](http://images.novusbio.com/fullsize/STAT1-Antibody-15H3-Western-Blot-NBP1-48013-img0001.jpg "Western Blot: STAT1 Antibody (15H3) [NBP1-48013] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY STAT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STAT1.")
![Immunocytochemistry/Immunofluorescence: STAT1 Antibody (15H3) [NBP1-48013] Staining of COS7 cells transiently transfected by pCMV6-ENTRY STAT1.](http://images.novusbio.com/fullsize/STAT1-Antibody-15H3-Immunocytochemistry-Immunofluorescence-NBP1-48013-img0006.jpg)
![Immunohistochemistry-Paraffin: STAT1 Antibody (15H3) [NBP1-48013] - Staining of paraffin-embedded Carcinoma of thyroid tissue using anti-STAT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/STAT1-Antibody-15H3-Immunohistochemistry-Paraffin-NBP1-48013-img0005.jpg)
![Immunohistochemistry-Paraffin: STAT1 Antibody (15H3) [NBP1-48013] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-STAT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/STAT1-Antibody-15H3-Immunohistochemistry-Paraffin-NBP1-48013-img0002.jpg)
![Immunohistochemistry-Paraffin: STAT1 Antibody (15H3) [NBP1-48013] - Staining of paraffin-embedded Carcinoma of bladder tissue using anti-STAT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/STAT1-Antibody-15H3-Immunohistochemistry-Paraffin-NBP1-48013-img0003.jpg)
![Immunohistochemistry-Paraffin: STAT1 Antibody (15H3) [NBP1-48013] - Staining of paraffin-embedded Carcinoma of pancreas tissue using anti-STAT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/STAT1-Antibody-15H3-Immunohistochemistry-Paraffin-NBP1-48013-img0004.jpg)
![Immunoprecipitation: STAT1 Antibody (SM1) [NB500-514] - Fig. 1: Induction of phosphorylation of STAT1 at Ser727 in human malignant melanoma cells (short-term culture derived from a patient) in response to interferons. Subconfluent cells were serum-starved before exposure to activation dosages of IFN-gamma (10 ng/ml) and IFN-alpha (1000 IU/ml and 5000 IU/ml). Western blotting analysis of cell extracts shows detection of phosphorylated STAT1 (Ser727) by the antibody PSM1 (upper panel) and total STAT1 level by the antibody SM1 (lower panel).](http://images.novusbio.com/fullsize/STAT1-Antibody-SM1-Immunoprecipitation-NB500-514-img0001.jpg "Immunoprecipitation: STAT1 Antibody (SM1) [NB500-514] - Fig. 1: Induction of phosphorylation of STAT1 at Ser727 in human malignant melanoma cells (short-term culture derived from a patient) in response to interferons. Subconfluent cells were serum-starved before exposure to activation dosages of IFN-gamma (10 ng/ml) and IFN-alpha (1000 IU/ml and 5000 IU/ml). Western blotting analysis of cell extracts shows detection of phosphorylated STAT1 (Ser727) by the antibody PSM1 (upper panel) and total STAT1 level by the antibody SM1 (lower panel).")
![Western Blot: STAT1 [p Ser727] Antibody [NB100-82209] - Analysis of extracts from Hela cells using STAT1(Phospho-Ser727) antibody](http://images.novusbio.com/fullsize/STAT1-[p-Ser727]-Antibody-Western-Blot-NB100-82209-img0001.jpg "Western Blot: STAT1 [p Ser727] Antibody [NB100-82209] - Analysis of extracts from Hela cells using STAT1(Phospho-Ser727) antibody")
![Immunocytochemistry/Immunofluorescence: STAT1 [p Ser727] Antibody [NB100-82209] - Staining of methanol-fixed Hela cells using STAT1(Phospho-Ser727) antibody.](http://images.novusbio.com/fullsize/STAT1-[p-Ser727]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-82209-img0002.jpg)
![Immunohistochemistry-Paraffin: STAT1 [p Ser727] Antibody [NB100-82209] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NB100-82209 (left) or the same antibody preincubated with blocking peptide(right)](http://images.novusbio.com/fullsize/STAT1-[p-Ser727]-Antibody-Immunohistochemistry-NB100-82209-img0003.jpg)