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![Western Blot: Follistatin-like 1/FSTL1 Antibody [NBP1-83425] - Analysis in human cell lines U2OS and Caco-2 using Anti-FSTL1 antibody. Corresponding FSTL1 RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.](http://images.novusbio.com/fullsize/Follistatin-like-1-FSTL1-Antibody-Western-Blot-NBP1-83425-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Follistatin-like 1/FSTL1 Antibody [NBP1-83425] - Analysis in human cell lines U2OS and Caco-2 using Anti-FSTL1 antibody. Corresponding FSTL1 RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Follistatin-like 1/FSTL1 Antibody [NBP1-83425] - Staining of human cell line U-2 OS shows localization to cytosol & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Follistatin-like-1-FSTL1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83425-img0007.jpg)
![Immunohistochemistry-Paraffin: Follistatin-like 1/FSTL1 Antibody [NBP1-83425] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Follistatin-like-1-FSTL1-Antibody-Immunohistochemistry-Paraffin-NBP1-83425-img0013.jpg)
![Immunohistochemistry-Paraffin: Follistatin-like 1/FSTL1 Antibody [NBP1-83425] - Staining of human testis shows moderate cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/Follistatin-like-1-FSTL1-Antibody-Immunohistochemistry-Paraffin-NBP1-83425-img0010.jpg)
![Immunohistochemistry-Paraffin: Follistatin-like 1/FSTL1 Antibody [NBP1-83425] - Staining of human pancreas shows moderate cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/Follistatin-like-1-FSTL1-Antibody-Immunohistochemistry-Paraffin-NBP1-83425-img0011.jpg)
![Immunohistochemistry-Paraffin: Follistatin-like 1/FSTL1 Antibody [NBP1-83425] - Staining of human urinary bladder shows weak membranous positivity in urothelial cells.](http://images.novusbio.com/fullsize/Follistatin-like-1-FSTL1-Antibody-Immunohistochemistry-Paraffin-NBP1-83425-img0012.jpg)
![Western Blot: FSTL1 Antibody [NBP2-16539] - Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/FSTL1-Antibody-Western-Blot-NBP2-16539-img0001.jpg "Western Blot: FSTL1 Antibody [NBP2-16539] - Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE gel, diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: FSTL1 Antibody [NBP2-16539] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/FSTL1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16539-img0003.jpg)
![Immunohistochemistry-Paraffin: FSTL1 Antibody [NBP2-16539] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/FSTL1-Antibody-Immunohistochemistry-Paraffin-NBP2-16539-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Follistatin-like 1/FSTL1 Antibody (009) [NBP2-89709] - Staining of Human FSTL1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human FSTL1 monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated goat Anti-rabbit IgG secondary antibody (green), countstained with DAPI (blue).Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/Follistatin-like-1-FSTL1-Antibody-009-Immunocytochemistry-Immunofluorescence-NBP2-89709-img0002.jpg "Immunocytochemistry/Immunofluorescence: Follistatin-like 1/FSTL1 Antibody (009) [NBP2-89709] - Staining of Human FSTL1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human FSTL1 monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated goat Anti-rabbit IgG secondary antibody (green), countstained with DAPI (blue).Positive staining was localized to cytoplasm.")
![Flow Cytometry: Follistatin-like 1/FSTL1 Antibody (009) [NBP2-89709] - Analysis of Human FSTL1 expression on HeLa cells . The cells were treated ,stained with purified anti-Human FSTL1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/Follistatin-like-1-FSTL1-Antibody-009-Flow-Cytometry-NBP2-89709-img0001.jpg)