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![Western Blot: Chicken anti-Rabbit IgG (H+L) Secondary Antibody [NB120-6831] - Western Blot: Chicken anti-Rabbit IgG (H+L) Secondary Antibody [Rhodamine] [NB120-6826] - Lane 1: Rabbit IgG. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Rhodamine chicken secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 55 kDa for Rabbit IgG.](http://images.novusbio.com/fullsize/Chicken-anti-Rabbit-IgG-H+L-Secondary-Antibody-Western-Blot-NB120-6831-img0002.jpg "Western Blot: Chicken anti-Rabbit IgG (H+L) Secondary Antibody [NB120-6831] - Western Blot: Chicken anti-Rabbit IgG (H+L) Secondary Antibody [Rhodamine] [NB120-6826] - Lane 1: Rabbit IgG. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Rhodamine chicken secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 55 kDa for Rabbit IgG.")
![Western Blot: Chicken anti-Rabbit IgG (H+L) Secondary Antibody [NB120-6831] - Lane 1: Rabbit IgG. Load: 50 ug per lane. Secondary antibody: Texas Red (TM) chicken secondary antibody at 1:1,000 for 60 min at RT. Block for30 min at RT. Predicted/Observed size: 55 kDa for Rabbit IgG.](http://images.novusbio.com/fullsize/Chicken-anti-Rabbit-IgG-H+L-Secondary-Antibody-Western-Blot-NB120-6831-img0006.jpg)
![Western Blot: Chicken anti-Rabbit IgG (H+L) Secondary Antibody [NB120-6831] - Chicken anti-Rabbit IgG (H+L) Secondary Antibody [NB120-6831] - Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment. Lane 4: Rabbit IgM Whole Molecule. Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (CHICKEN) Antibody 1:1,000 for 60 min at RT. Secondary antibody: Anti-Chicken IgG (GOAT) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.](http://images.novusbio.com/fullsize/Chicken-anti-Rabbit-IgG-H+L-Secondary-Antibody-Western-Blot-NB120-6831-img0004.jpg)
![Dot Blot: Chicken anti-Rabbit IgG (H+L) Secondary Antibody [NB120-6831] - Dot Blot of Chicken anti-Rabbit IgG (H+L) Secondary Antibody [Fluorescein] (Pre-absorbed). Antigen: Rabbit IgG Load: Lane 1 - 50 ng Lane 2 - 16.67 ng Lane 3 - 5.56 ng Lane 4 - 1.85 ng Lane 5 - 0.62 ng. Incubated for 1hr at RT; blocked for 60 min at RT. Image from the FITC version of this antibody.](http://images.novusbio.com/fullsize/Chicken-anti-Rabbit-IgG-H+L-Secondary-Antibody-Dot-Blot-NB120-6831-img0005.jpg)
![Western Blot: Chicken anti-Human IgG (H+L) Secondary Antibody [NB120-6713] - Lane 1: Human IgG. Load: 100 ug per lane. Secondary antibody: Human IgG (H&L) Antibody Peroxidase conjugated at 1:1,000 for 60 min at RT. Blocking buffer for 30 min at RT. Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa. Image using the HRP format of this antibody.](http://images.novusbio.com/fullsize/Chicken-anti-Human-IgG-H+L-Secondary-Antibody-Western-Blot-NB120-6713-img0003.jpg "Western Blot: Chicken anti-Human IgG (H+L) Secondary Antibody [NB120-6713] - Lane 1: Human IgG. Load: 100 ug per lane. Secondary antibody: Human IgG (H&L) Antibody Peroxidase conjugated at 1:1,000 for 60 min at RT. Blocking buffer for 30 min at RT. Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa. Image using the HRP format of this antibody.")
![Western Blot: Chicken anti-Human IgG (H+L) Secondary Antibody [NB120-6713] - Lane 1: Human IgG Load: 100 ug per lane Secondary antibody: Human IgG (H&L) Antibody Alkaline Phosphatase conjugated at 1:1,000 for 60 min at RT. Blocking buffer for 30 min at RT Predicted/Observed size: 55 and 28 kDa, 55 kDa. Image using the Alkaline Phosphatase format of this antibody.](http://images.novusbio.com/fullsize/Chicken-anti-Human-IgG-H+L-Secondary-Antibody-Western-Blot-NB120-6713-img0002.jpg)
![Dot Blot: Chicken anti-Human IgG (H+L) Secondary Antibody [NB120-6713] - Dot Blot of Chicken anti-Human IgG (H+L) Secondary Antibody [FITC]. Lane 1: 100ng. Lane 2: 33.3ng. Lane 3: 11.1ng. Lane 4: 3.7ng. Lane 5: 1.23ng. Chicken anti-Human IgG (H+L) Secondary Antibody [FITC] 1ug/ml. Blocked for 30 min at RT. Image from the FITC version of this antibody.](http://images.novusbio.com/fullsize/Chicken-anti-Human-IgG-H+L-Secondary-Antibody-Dot-Blot-NB120-6713-img0001.jpg)
![ELISA: Chicken anti-Human IgG (H+L) Secondary Antibody [NB120-6713] - ELISA Results of Purified Chicken anti-Human IgG (H+L) Secondary antibody tested against purified Human IgG. Each well was coated in duplicate with 10 ug of Human IgG. The working dilution of Anti-Human IgG is 1:110,000. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking buffer, Goat Anti-Chicken IgG mx10 HRP conjugated and TMB substrate.](http://images.novusbio.com/fullsize/Chicken-anti-Human-IgG-H+L-Secondary-Antibody-ELISA-NB120-6713-img0006.jpg)
![Western Blot: PERK Antibody [NBP3-12193] - WB of NBP3-12193 with adrenal cells. Left-Chicken serum antibodies. Right- Total NBP3-12193 IGY. Apparent MW is 135 kDa.](http://images.novusbio.com/fullsize/PERK-Antibody-Western-Blot-NBP3-12193-img0001.jpg "Western Blot: PERK Antibody [NBP3-12193] - WB of NBP3-12193 with adrenal cells. Left-Chicken serum antibodies. Right- Total NBP3-12193 IGY. Apparent MW is 135 kDa.")
![Immunohistochemistry-Paraffin: PERK Antibody [NBP3-12193] - Mouse cerebellum. 1:100 in IHC blobking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification on Leica 40X DM4000B. FFPE section.](http://images.novusbio.com/fullsize/PERK-Antibody-Immunohistochemistry-Paraffin-NBP3-12193-img0002.jpg)
![Western Blot: Chicken anti-Rat IgG (H+L) Secondary Antibody [NB120-6838] - Western Blot of Chicken anti-Rat IgG (H+L) Secondary antibody. Lane M: 3 ul Molecular Ladder. Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment. Lane 3: Rat IgG Fab Fragment. Lane 4: Rat IgM Whole Molecule. Lane 5: Rat Serum. All samples were reduced. Load: 50 ng per lane. Block for 30 min at RT. Primary Antibody: Chicken anti-Rat IgG (H+L) Secondary antibody 1:1,000 for 60 min at RT. Secondary Antibody: Anti-Chicken IgG (GOAT) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Obsevered Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.](http://images.novusbio.com/fullsize/Chicken-anti-Rat-IgG-H+L-Secondary-Antibody-Western-Blot-NB120-6838-img0008.jpg "Western Blot: Chicken anti-Rat IgG (H+L) Secondary Antibody [NB120-6838] - Western Blot of Chicken anti-Rat IgG (H+L) Secondary antibody. Lane M: 3 ul Molecular Ladder. Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment. Lane 3: Rat IgG Fab Fragment. Lane 4: Rat IgM Whole Molecule. Lane 5: Rat Serum. All samples were reduced. Load: 50 ng per lane. Block for 30 min at RT. Primary Antibody: Chicken anti-Rat IgG (H+L) Secondary antibody 1:1,000 for 60 min at RT. Secondary Antibody: Anti-Chicken IgG (GOAT) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Obsevered Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.")
![Dot Blot: Chicken anti-Rat IgG (H+L) Secondary Antibody [NB120-6838] - 0.62 ng.Primary antibody: n/a.Secondary antibody: Chicken anti-Rat IgG (H+L) Secondary Antibody [FITC] at 1:1000 for 60 min at RT.Block: Fluorescent blocking buffer for 1 HR at RT.. Using the FITC format of this antibody.](http://images.novusbio.com/fullsize/Chicken-anti-Rat-IgG-H+L-Secondary-Antibody-Dot-Blot-NB120-6838-img0007.jpg)