| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-105SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-104SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-101SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80687 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12073-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12072-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12073-0.2mg | |||
| Azide and BSA Free | NBP3-12073-0.025mg | |||
![Suppressive effects of Ucn1 on foam cell formation and related protein expression in human monocyte-derived macrophages.Human monocytes were incubated for 7 days with RPMI-1640 supplemented with 10% human serum and the indicated concentrations of Ucn1, followed by a 19 h-incubation with 50 ug/ml oxLDL in the presence of 0.1 mmol/l [3H]oleate. Intracellular CE accumulation was determined from the radioactivity of cholesterol-[3H]oleate. Otherwise, before the addition of oxLDL, cells were harvested and subjected to immunoblotting analyses for CD36, ACAT1, or ABCA1. beta-Actin served as a loading control. Data are expressed as means +/- SEM from 4-6 independent experiments with monocytes from 4-6 different donors. Baseline (1 fold) = 8.4+/-2.2 nmol/mg cell protein. *P<0.05, +P<0.001 vs. 0 nmol/l of Ucn1. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0110866), licensed under a CC-BY licence.](http://images.novusbio.com/fullsize/nb400-105_rabbit-polyclonal-abca1-antibody-291220231147216.jpg)
![Simple Western: SR-BI Antibody [NB400-104] - Image shows a specific band for SR-BI in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/SR-BI-Antibody-Simple-Western-NB400-104-img0014.jpg "Simple Western: SR-BI Antibody [NB400-104] - Image shows a specific band for SR-BI in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Western Blot: SR-BI Antibody [NB400-104] - SR-BI antibody was tested in human adrenal cell lysate.](http://images.novusbio.com/fullsize/SR-BI-Antibody-Western-Blot-NB400-104-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: SR-BI Antibody [NB400-104] - SR-BI antibody was tested in human fibroblast samples fixed in 4% PFA and permeabilized in PBS (0.2% Tween). Primary incubation overnight at 4C using a 1:100 dilution in PBS (0.1% Tween) with 1% BSA. Secondary antibody is anti-rabbit conjugate to Alexa Fluor 488. SR-BI is shown in green and nucleus in blue (Hoescht 33342 stain).](http://images.novusbio.com/fullsize/SR-BI-Antibody-Immunocytochemistry-Immunofluorescence-NB400-104-img0030.jpg)
![Flow Cytometry: SR-BI Antibody [NB400-104] - An intracellular stain was performed on HeLa cells with SR-BI antibody NB400-104AF488 (blue) and a matched isotype control NBP2-24893AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/SR-BI-Antibody-Flow-Cytometry-NB400-104-img0026.jpg)
![Western Blot: SR-BI Antibody [NB400-104] - Detection of SR-BI in rat H4IIE total cell lysates and plasma membrane proteins. Photo courtesy of product review by verified customer.](http://images.novusbio.com/fullsize/SR-BI-Antibody-Western-Blot-NB400-104-img0013.jpg)
![Immunohistochemistry: SR-BI Antibody [NB400-104] - Immunolocalization of SR-BI in adult mink testis using NB400-104. SR-BI labeling is visible at the surface and along the outline of the large vacuole. Photo courtesy of R.M. Pelletier, University of Montreal.](http://images.novusbio.com/fullsize/SR-BI-Antibody-Immunohistochemistry-NB400-104-img0011.jpg)
![Immunohistochemistry-Paraffin: SR-BI Antibody [NB400-104] - FFPE tissue section of mouse liver using SR-BI antibody (Lot 8310) at 1:300 dilution with HRP-DAB detection and hematoxylin counterstaining. The antibody generated a specific membrane signal of SR-BI protein in the murine hepatocytes.](http://images.novusbio.com/fullsize/SR-BI-Antibody-Immunohistochemistry-Paraffin-NB400-104-img0022.jpg)
![<b>Genetic Strategies Validation. </b>Knockdown Validated: SR-BI Antibody [NB400-104] - HCV replication was increased by CD36. Silence of CD36 decreased HCV infection and replication (n = 3). Huh7.5 cells were transfected with 0.2 u g CD36-HA plasmid or with 150 pmol siRNA for CD36 (B) for 48 hrs, and then were washed and infected with HCV (150 IU/cell) for 2 hrs, followed by washing and continuously incubating. Intracellular HCV RNA and proteins were detected in 96 hrs, and the cytotoxicity of siRNAs was measured in 96-wells plate with a MTT assay in B. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep21808) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SR-BI-Antibody-Western-Blot-NB400-104-img0034.jpg)
![Immunohistochemistry-Paraffin: SR-BI Antibody [NB400-104] - Staining of human adrenal cortex.](http://images.novusbio.com/fullsize/SR-BI-Antibody-Immunohistochemistry-Paraffin-NB400-104-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: SR-BI Antibody [NB400-104] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-SR-BI at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/SR-BI-Antibody-Immunocytochemistry-Immunofluorescence-NB400-104-img0029.jpg)
![Immunohistochemistry-Paraffin: SR-BI Antibody [NB400-104] - FFPE tissue section of mouse liver using SR-BI antibody (Lot R-4) at 1:300 dilution with HRP-DAB detection and hematoxylin counterstaining. The antibody generated mainly a membranous signal of SR-BI protein in the murine hepatocytes.](http://images.novusbio.com/fullsize/SR-BI-Antibody-Immunohistochemistry-Paraffin-NB400-104-img0023.jpg)
![Flow Cytometry: SR-BI Antibody [NB400-104] - Analysis of Huh7 and HepG2 cells using SR-B1 antibody NB400-104. Courtesy of Bruno Sainz, Jr., PhD, University of Illinois at Chicago.](http://images.novusbio.com/fullsize/SR-BI-Antibody-Flow-Cytometry-NB400-104-img0004.jpg)
![Knockout Validated: SR-BI Antibody [NB400-101] - Immunofluorescent analysis of SR-BI localization in aorta of Tie2-Scarb1 x Scarb1-KO and Scarb1-KO mice. Mouse aorta sections were stained for SR-BI (a and d, red). In several EC stained for SR-B1 red color was present on both apical and basolateral sides. There is no red signal in aorta from Scarb1-KO mice. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/bmri/2015/607120/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SR-BI-Antibody-Knockout-Validated-NB400-101-img0018.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: SR-BI Antibody [NB400-101] - Immunofluorescent analysis of SR-BI localization in aorta of Tie2-Scarb1 x Scarb1-KO and Scarb1-KO mice. Mouse aorta sections were stained for SR-BI (a and d, red). In several EC stained for SR-B1 red color was present on both apical and basolateral sides. There is no red signal in aorta from Scarb1-KO mice. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/bmri/2015/607120/) licensed under a CC-BY license.")
![<b>Genetic Strategies Validation. </b>Simple Western: SR-BI Antibody [NB400-101] - Lane view shows a specific band for SR-BI in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/SR-BI-Antibody-Simple-Western-NB400-101-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: SR-BI Antibody [NB400-101] - Immunofluorescent analysis of SR-BI localization in liver of normal, Tie2-Scarb1 x Scarb1-KO, and Scarb1-KO mice. Liver sections from normal (b) and Tie2-Scarb1 x Scarb1-KO mice (c) were stained for SR-BI (red) and cytokeratin 8-18 (green).Merge image for normal mouse (b) demonstrates strong presence of SR-BI in hepatocytes (b, yellow signal) and absence of detectable level of SR-BI protein in liver of Tie2-Scarb1 x Scarb1-KO mice (c). In Scarb1-KO mice there was no detectable level of SR-BI protein (d, staining for SR-BI). Blue = DAPI. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/bmri/2015/607120/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SR-BI-Antibody-Knockout-Validated-NB400-101-img0011.jpg)
![Western Blot: SR-BI Antibody [NB400-101] - Detection of SR-BI in mouse liver lysate (20 ug) using NB 400-101. ECL detection 5 seconds.](http://images.novusbio.com/fullsize/SR-BI-Antibody-Western-Blot-NB400-101-img0005.jpg)
![Flow (Intracellular): SR-BI Antibody [NB400-101] - An intracellular stain was performed on HeLa cells with NB400-101AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/SR-BI-Antibody-Flow-Intracellular-NB400-101-img0010.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: SR-BI Antibody [NB400-101] - Western blot and Coomassie stain of membrane fractions isolated from livers of normal C57Bl/6N (lane 1), Scarb1-KO (lane 2), and LIV11-SCARB1 x Scarb1-KO (lane 3) mice. 20 ug of membrane protein was loaded into each lane. (a)Western blot (anti-SR-BI antibody). (b) Coomassie stain (loading control for Western blot) encompassing the same MW region as SR-BI. Aliquots from the same tube were loaded for the Western blot and for the Coomassie-stained gel, which shows comparable loading between the three samples. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/bmri/2015/607120/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SR-BI-Antibody-Knockout-Validated-NB400-101-img0014.jpg)
![Western Blot: SR-BI Antibody [NB400-101] - Western blot analysis of ABCA1 and SR-B1 proteins from WT and Osbpl8KO mouse liver. The blots were probed with anti-beta-actin as a loading control. Densitometric quantification of the Western blot data is shown on the right. The results were normalized against beta-actin. The data represents mean +/- s.e.m. (n = 4). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0058856), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SR-BI-Antibody-Western-Blot-NB400-101-img0020.jpg)
![Immunocytochemistry/Immunofluorescence: SR-BI Antibody [NB400-101] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-SR-BI at 2 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/SR-BI-Antibody-Immunocytochemistry-Immunofluorescence-NB400-101-img0009.jpg)
![Immunohistochemistry-Paraffin: SR-BI Antibody [NB400-101] - SR-B1 was detected in immersion fixed paraffin-embedded sections of human liver using rabbit anti-human antibody (Catalog # NB400-101) at 1:300 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-rabbit HRP polymer detection reagent (Catalog # VC003) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a Bio-techne brand.](http://images.novusbio.com/fullsize/SR-BI-Antibody-Immunohistochemistry-Paraffin-NB400-101-img0008.jpg)
![Western Blot: SR-BI Antibody [NB400-101] - Western blot analysis of ABCA1 and SR-B1 proteins from WT and Osbpl8KO mouse liver. The blots were probed with anti-beta-actin as a loading control. Densitometric quantification of the Western blot data is shown on the right. The results were normalized against beta-actin. The data represents mean +/- s.e.m. (n?=?4). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0058856), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SR-BI-Antibody-Western-Blot-NB400-101-img0015.jpg)
![Western Blot: SR-BI Antibody [NB400-101] - Western blot analysis of ABCA1 and SR-B1 proteins from WT and Osbpl8KO mouse liver. The blots were probed with anti-beta-actin as a loading control. Densitometric quantification of the Western blot data is shown on the right. The results were normalized against beta-actin. The data represents mean +/- s.e.m. (n=4). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0058856), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SR-BI-Antibody-Western-Blot-NB400-101-img0019.jpg)
![Western Blot: SR-BI Antibody [NB400-113] - Detection of SR-BI (80kDa) in mouse testis lysate total protein using NB400-113.](http://images.novusbio.com/fullsize/SR-BI-Antibody-Western-Blot-NB400-113-img0009.jpg "Western Blot: SR-BI Antibody [NB400-113] - Detection of SR-BI (80kDa) in mouse testis lysate total protein using NB400-113.")
![Immunocytochemistry/Immunofluorescence: SR-BI Antibody [NB400-113] - Antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/SR-BI-Antibody-Immunocytochemistry-Immunofluorescence-NB400-113-img0006.jpg)
![Immunohistochemistry-Paraffin: SR-BI Antibody [NB400-113] - Analysis of a FFPE section of human adrenal gland tissue using 1:100 dilution of SR-BI antibody. The staining was developed using HRP-DAB based detection method and the nuclei of the cells were counterstained with hematoxylin. This antibody generated a specific staining of SR-BI/SCARB1 in the glandular cells. The staining was membrane-cytoplasmic in the zona glomerulosa cells while the signal was primarily localized to the membranes of the cells in the zona fasciculata and zona reticularis layers of the adrenal cortex.](http://images.novusbio.com/fullsize/SR-BI-Antibody-Immunohistochemistry-Paraffin-NB400-113-img0008.jpg)
![Immunohistochemistry-Paraffin: SR-BI Antibody [NB400-113] - Analysis of a FFPE section of human adrenal gland tissue using 1:100 dilution of SR-BI antibody. The staining was developed using HRP-DAB based detection method and the nuclei of the cells were counterstained using hematoxylin. The representative section shows SR-BI/SCARB1 positivity in the glandular cells and the staining was mainly localized to the membranes of the cells.](http://images.novusbio.com/fullsize/SR-BI-Antibody-Immunohistochemistry-Paraffin-NB400-113-img0007.jpg)
![Western Blot: SR-BI Antibody [NB400-113] - Cell surface expression of SR-BI in renal carcinoma cells. Western blot analysis of SR-BI in total cell lysates (left) and on the cell surface (right) in 786-O and 786-O-VHL cells. The Western blots were probed with anti-SR-BI (82 kDa), anti-TBP (40 kDa, used as a control for intracellular protein expression), and anti-Na+/K+-ATPase (110 kDa, used as a control for cell surface protein expression). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30173145/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nb400-113_rabbit-polyclonal-sr-bi-antibody-2552023151494.jpg)
![Western Blot: SR-BI/SR-BII Antibody [NB400-134] - Detection of RED-1 in 80 ug of total mouse liver lysates. Lane 1: wild-type mice, Lane 2: SR-BI deficient mice.](http://images.novusbio.com/fullsize/SR-BI-SR-BII-Antibody-Western-Blot-NB400-134-img0005.jpg "Western Blot: SR-BI/SR-BII Antibody [NB400-134] - Detection of RED-1 in 80 ug of total mouse liver lysates. Lane 1: wild-type mice, Lane 2: SR-BI deficient mice.")
![Immunocytochemistry/Immunofluorescence: SR-BI/SR-BII Antibody [NB400-134] - SR-BI/SR-BII antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/SR-BI-SR-BII-Antibody-Immunocytochemistry-Immunofluorescence-NB400-134-img0008.jpg)
![Immunohistochemistry-Paraffin: SR-BI/SR-BII Antibody [NB400-134] - IHC analysis of formalin-fixed paraffin-embedded tissue section of normal human liver using 5 ug/ml concentration of SR-BI/SR-BII antibody. Specific and expected granular membrane-cytoplasmic staining was observed in the hepatocytes. [40X Magnification]](http://images.novusbio.com/fullsize/SR-BI-SR-BII-Antibody-Immunohistochemistry-Paraffin-NB400-134-img0009.jpg)
![Flow Cytometry: SR-BI/SR-BII Antibody [NB400-134] - SR-BI/SR-BII antibody was tested at 1:400 in NIH-3T3 cells using an Alexa Fluor 488 secondary (shown in purple). M1 is defined by unstained cells.](http://images.novusbio.com/fullsize/SR-BI-SR-BII-Antibody-Flow-Cytometry-NB400-134-img0006.jpg)
![Immunoprecipitation: Cytochrome c Antibody (6H2.B4) [NBP2-24872] - Immunoprecitation/analysis using cytochrome c antibodies. Two micrograms of cytochrome c antibody clone 6H2.B4 (lane 1) or control IgG antibody (lane 2) were used to immunoprecipitate cytochrome c from 1 x 10^6 HeLa cells. The immunoprecipitated protein was resolved by SDS-PAGE and probed with cytochrome antibody clone 7H8.2C12 .](http://images.novusbio.com/fullsize/Cytochrome-c-Antibody-6H2-B4-Immunoprecipitation-NBP2-24872-img0004.jpg "Immunoprecipitation: Cytochrome c Antibody (6H2.B4) [NBP2-24872] - Immunoprecitation/analysis using cytochrome c antibodies. Two micrograms of cytochrome c antibody clone 6H2.B4 (lane 1) or control IgG antibody (lane 2) were used to immunoprecipitate cytochrome c from 1 x 10^6 HeLa cells. The immunoprecipitated protein was resolved by SDS-PAGE and probed with cytochrome antibody clone 7H8.2C12 .")
![Immunoprecipitation: Cytochrome c Antibody (6H2.B4) [NBP2-24872] - Cytochrome c was immunoprecipitated from Hela cell extract (1% NP-40) using 2-4 ug 6H2.B4 antibody/1 x107 cell equivalents.](http://images.novusbio.com/fullsize/Cytochrome-c-Antibody-6H2-B4-Immunoprecipitation-NBP2-24872-img0005.jpg)
![Western Blot: SR-BI Antibody (185-1G2 (B467)) - Chimeric [NBP3-12073] - Human peripheral blood monocyte cell lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of 185-1G2 (B467) (NBP3-12073) at 0.03 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/SR-BI-Antibody-185-1G2-B467-Chimeric-Western-Blot-NBP3-12073-img0001.jpg "Western Blot: SR-BI Antibody (185-1G2 (B467)) - Chimeric [NBP3-12073] - Human peripheral blood monocyte cell lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of 185-1G2 (B467) (NBP3-12073) at 0.03 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: SR-BI Antibody (185-1G2 (B467)) - Chimeric [NBP3-12073] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 185-1G2 (B467) (NBP3-12073) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12073, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/SR-BI-Antibody-185-1G2-B467-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12073-img0002.jpg)
![SR-BI Antibody (185-1G2 (B467)) [mFluor Violet 610 SE] [NBP3-12073MFV610] - Vial of mFluor Violet 610 conjugated antibody. mFluor Violet 610 is optimally excited at 421 nm by the Violet laser (405 nm) and has an emission maximum of 613 nm.](http://images.novusbio.com/fullsize/nbp3-12073mfv610_rabbit-sr-bi-mab-185-1g2-b467-mfluor-violet-610-se-6720239175331.png "SR-BI Antibody (185-1G2 (B467)) [mFluor Violet 610 SE] [NBP3-12073MFV610] - Vial of mFluor Violet 610 conjugated antibody. mFluor Violet 610 is optimally excited at 421 nm by the Violet laser (405 nm) and has an emission maximum of 613 nm.")