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![Immunohistochemistry-Paraffin: TPPP/p25 Antibody [NBP2-34031] - Analysis in human cerebral cortex and liver tissues using NBP2-34031 antibody. Corresponding TPPP RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/TPPP-p25-Antibody-Immunohistochemistry-Paraffin-NBP2-34031-img0016.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: TPPP/p25 Antibody [NBP2-34031] - Analysis in human cerebral cortex and liver tissues using NBP2-34031 antibody. Corresponding TPPP RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: TPPP/p25 Antibody [NBP2-34031] - Staining of human cerebral cortex, liver, lower gastrointestinal and pancreas using Anti-TPPP antibody NBP2-34031 (A) shows similar protein distribution across tissues to independent antibody NBP1-80962 (B).](http://images.novusbio.com/fullsize/TPPP-p25-Antibody-Immunohistochemistry-Paraffin-NBP2-34031-img0015.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: TPPP/p25 Antibody [NBP2-34031] - TPPP/p25, a microtubule-stabilising protein, is a target of linc00899 in regulation of mitosis. Representative western blot of TPPP/p25 levels after depletion of linc00899. beta-Tubulin and p150 were used as two loading controls. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-020-14978-7), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TPPP-p25-Antibody-Western-Blot-NBP2-34031-img0021.jpg)
![Immunocytochemistry/Immunofluorescence: TPPP/p25 Antibody [NBP2-34031] - Staining of human cell line A549 shows localization to cytosol & mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/TPPP-p25-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-34031-img0005.jpg)
![Immunohistochemistry-Paraffin: TPPP/p25 Antibody [NBP2-34031] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/TPPP-p25-Antibody-Immunohistochemistry-Paraffin-NBP2-34031-img0020.jpg)
![Western Blot: TPPP/p25 Antibody [NBP2-34031] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral cortex tissue.](http://images.novusbio.com/fullsize/TPPP-p25-Antibody-Western-Blot-NBP2-34031-img0004.jpg)
![Immunohistochemistry-Paraffin: TPPP/p25 Antibody [NBP2-34031] - Staining of human cerebral cortex shows strong cytoplasmic and nuclear positivity in glial cells.](http://images.novusbio.com/fullsize/TPPP-p25-Antibody-Immunohistochemistry-Paraffin-NBP2-34031-img0017.jpg)
![Immunohistochemistry-Paraffin: TPPP/p25 Antibody [NBP2-34031] - Staining of human lower gastrointestinal shows weak cytoplasmic positivity in peripheral ganglion.](http://images.novusbio.com/fullsize/TPPP-p25-Antibody-Immunohistochemistry-Paraffin-NBP2-34031-img0018.jpg)
![Immunohistochemistry-Paraffin: TPPP/p25 Antibody [NBP2-34031] - Staining of human pancreas shows moderate cytoplasmic positivity in islets of Langerhans.](http://images.novusbio.com/fullsize/TPPP-p25-Antibody-Immunohistochemistry-Paraffin-NBP2-34031-img0019.jpg)
![Western Blot: TPPP/p25 Antibody [NBP1-49833] - (0.001ug/ml) staining of Mouse (A) and Rat (B) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence](http://images.novusbio.com/fullsize/TPPP-p25-Antibody-Western-Blot-NBP1-49833-img0006.jpg "Western Blot: TPPP/p25 Antibody [NBP1-49833] - (0.001ug/ml) staining of Mouse (A) and Rat (B) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence")
![Immunohistochemistry-Paraffin: TPPP/p25 Antibody [NBP1-49833] - Staining of Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/TPPP-p25-Antibody-Immunohistochemistry-Paraffin-NBP1-49833-img0008.jpg)
![Western Blot: TPPP/p25 Antibody [NBP1-49833] - (0.001ug/ml) Analysis of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/TPPP-p25-Antibody-Western-Blot-NBP1-49833-img0001.jpg)
![Immunohistochemistry-Paraffin: TPPP/p25 Antibody [NBP1-49833] - Negative Control showing staining of Human Cerebellum, with no primary antibody.](http://images.novusbio.com/fullsize/TPPP-p25-Antibody-Immunohistochemistry-Paraffin-NBP1-49833-img0005.jpg)
![Immunohistochemistry-Paraffin: TPPP/p25 Antibody [NBP1-49833] - (2ug/ml) staining of Human Cerebellum. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/TPPP-p25-Antibody-Immunohistochemistry-Paraffin-NBP1-49833-img0007.jpg)
![Western Blot: TPPP/p25 Antibody [NBP1-80962] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-592](http://images.novusbio.com/fullsize/TPPP-p25-Antibody-Western-Blot-NBP1-80962-img0005.jpg "Western Blot: TPPP/p25 Antibody [NBP1-80962] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-592")
![Immunohistochemistry-Paraffin: TPPP/p25 Antibody [NBP1-80962] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/TPPP-p25-Antibody-Immunohistochemistry-Paraffin-NBP1-80962-img0023.jpg)
![Immunohistochemistry-Paraffin: TPPP/p25 Antibody [NBP1-80962] - Staining of human cerebral cortex shows nuclear and cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/TPPP-p25-Antibody-Immunohistochemistry-Paraffin-NBP1-80962-img0012.jpg)
![Immunohistochemistry-Paraffin: TPPP/p25 Antibody [NBP1-80962] - Staining of human lymph node.](http://images.novusbio.com/fullsize/TPPP-p25-Antibody-Immunohistochemistry-Paraffin-NBP1-80962-img0013.jpg)
![Immunohistochemistry-Paraffin: TPPP/p25 Antibody [NBP1-80962] - Staining of human placenta shows no positivity in trophoblastic cells and weak cytoplasmic and nuclear staining in Hofbauer cells.](http://images.novusbio.com/fullsize/TPPP-p25-Antibody-Immunohistochemistry-Paraffin-NBP1-80962-img0014.jpg)
![Immunohistochemistry-Paraffin: TPPP/p25 Antibody [NBP1-80962] - Staining of human pancreas shows cytoplasmicand nuclear positivity in islets of Langerhans.](http://images.novusbio.com/fullsize/TPPP-p25-Antibody-Immunohistochemistry-Paraffin-NBP1-80962-img0015.jpg)
![Immunohistochemistry-Paraffin: TPPP/p25 Antibody [NBP1-80962] - Staining of human fallopian tube shows cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/TPPP-p25-Antibody-Immunohistochemistry-Paraffin-NBP1-80962-img0016.jpg)
![Immunohistochemistry-Paraffin: TPPP/p25 Antibody [NBP1-80962] - Staining of human liver.](http://images.novusbio.com/fullsize/TPPP-p25-Antibody-Immunohistochemistry-Paraffin-NBP1-80962-img0017.jpg)
![Immunohistochemistry-Paraffin: TPPP/p25 Antibody [NBP1-80962] - Staining of human colon.](http://images.novusbio.com/fullsize/TPPP-p25-Antibody-Immunohistochemistry-Paraffin-NBP1-80962-img0018.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: TPPP/p25 Antibody [NBP1-80962] - Staining of human cerebral cortex, liver, lower gastrointestinal and pancreas using Anti-TPPP antibody NBP1-80962 (A) shows similar protein distribution across tissues to independent antibody NBP2-34031 (B).](http://images.novusbio.com/fullsize/TPPP-p25-Antibody-Immunohistochemistry-Paraffin-NBP1-80962-img0019.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: TPPP/p25 Antibody [NBP1-80962] - Analysis in human cerebral cortex and liver tissues using NBP1-80962 antibody. Corresponding TPPP RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/TPPP-p25-Antibody-Immunohistochemistry-Paraffin-NBP1-80962-img0020.jpg)
![Immunohistochemistry-Paraffin: TPPP/p25 Antibody [NBP1-80962] - Staining of human cerebral cortex shows strong cytoplasmic and nuclear positivity in glial cells.](http://images.novusbio.com/fullsize/TPPP-p25-Antibody-Immunohistochemistry-Paraffin-NBP1-80962-img0021.jpg)
![Immunohistochemistry-Paraffin: TPPP/p25 Antibody [NBP1-80962] - Staining of human lower gastrointestinal shows moderate cytoplasmic positivity in peripheral ganglion.](http://images.novusbio.com/fullsize/TPPP-p25-Antibody-Immunohistochemistry-Paraffin-NBP1-80962-img0022.jpg)
![Western Blot: TPPP/p25 Antibody (3S5U9) [NBP3-16454] - Western blot analysis of extracts of various cell lines, using TPPP/p25 Rabbit mAb (NBP3-16454) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/TPPP-p25-Antibody-3S5U9-Western-Blot-NBP3-16454-img0003.jpg "Western Blot: TPPP/p25 Antibody (3S5U9) [NBP3-16454] - Western blot analysis of extracts of various cell lines, using TPPP/p25 Rabbit mAb (NBP3-16454) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: TPPP/p25 Antibody (3S5U9) [NBP3-16454] - Immunohistochemistry of paraffin-embedded mouse brain using TPPP/p25 Rabbit mAb (NBP3-16454) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/TPPP-p25-Antibody-3S5U9-Immunohistochemistry-Paraffin-NBP3-16454-img0002.jpg)
![Immunohistochemistry-Paraffin: TPPP/p25 Antibody (3S5U9) [NBP3-16454] - Immunohistochemistry of paraffin-embedded rat brain using TPPP/p25 Rabbit mAb (NBP3-16454) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/TPPP-p25-Antibody-3S5U9-Immunohistochemistry-Paraffin-NBP3-16454-img0001.jpg)
![Western Blot: TPPP/p25 Antibody [NBP2-20688] - A. 50 ug rat brain lysate/extract 15 % SDS-PAGETPPP antibody dilution: 1:1000](http://images.novusbio.com/fullsize/TPPP-p25-Antibody-Western-Blot-NBP2-20688-img0004.jpg "Western Blot: TPPP/p25 Antibody [NBP2-20688] - A. 50 ug rat brain lysate/extract 15 % SDS-PAGETPPP antibody dilution: 1:1000")
![Western Blot: TPPP/p25 Antibody [NBP2-20688] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/TPPP-p25-Antibody-Western-Blot-NBP2-20688-img0001.jpg)
![Western Blot: TPPP/p25 Antibody [NBP2-20688] - A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat2 whole cell lysate/extract 12 % SDS-PAGE TPPP antibody dilution: 1:20000](http://images.novusbio.com/fullsize/TPPP-p25-Antibody-Western-Blot-NBP2-20688-img0003.jpg)
![Western Blot: TPPP/p25 Antibody (SR1385) [NBP3-22044] - Western blot analysis using NBP3-22044 in SHSY5Y cell lysate.](http://images.novusbio.com/fullsize/nbp3-22044_rabbit-tppp-p25-mab-sr1385-672023106279.jpg "Western Blot: TPPP/p25 Antibody (SR1385) [NBP3-22044] - Western blot analysis using NBP3-22044 in SHSY5Y cell lysate.")